Objective Along with embedded software and hardware technical development, it has been used more and more extensive in the medical equipments. The cooperation communication can develop their function effectively between the multi service agents. Methods Firstly, the information of communication can be delivered in time and communicated of the service agent; secondly, the service agent can do a comprehension of cooperation information. Results The modern network communication and the computer technique are to meet the cooperation information of credibility in time. Conclusion The use of these advanced technologies, the collaborative framework of structure, coordination and collaboration protocol stack process can realize multi-service communications between the agents for the equipment and lay the foundation for systems development.

Objective To discuss the effect of 5-Aza-CdR on pancreatic carcinoma Capan-2 cell proliferation and PCDH8 gene expression.Methods Pancreatic carcinoma Capan-2 cells were treated with different doses of 5-Aza-CdR with or without gemcitabine,negative control group without drug,0.08μmol/L group with 0.08μmol/L 5-Aza-CdR,0.40 μmol/L group with 0.40 μmol/L 5-Aza-CdR,2.00 μmol/L group with 2.00 μmol/L 5-Aza-CdR,10 μmol/L group with 10 μmol/L 5-Aza-CdR,50 μmol/L group with 50 μmol/L 5-Aza-CdR. The inhibition ratio of Capan-2 cell proliferation were observed by MTT assay and PCDH8 gene and protein expression were detected by RT-PCR and Western blot. Results The inhibition ratio was increased with 5-Aza-CdR dose increasing(P<0.01),but decreased apparently with times extending(P<0.01). Inhibition ratio in 5-Aza-CdR and gemcitabine group was higher than those with only 5-Aza-CdR or gemcitabine groups(P<0.05 or P<0.01).The levels of PCDH8 gene and protein expression were increased significantly in 5-Aza-CdR treatment groups,with dose dependent (P <0.01 ).Conclusion 5-Aza-CdR can inhibit the proliferation of pancreatic carcinoma Capan-2 cell proliferation,and increase PCDH8 gene expression. The inhibition effect is strong when combined with gemcitabine.

Objective To investigate the clinical value of the combined detection of Golgi protein 73 (GP73),alpha-fetoprotein variant-L3 (AFP-L3)and transforming growth factorβ1(TGFβ1)in the early diagnosis of hepatocellular carcinoma (HCC).Meth-ods 48 cases of healthy control,42 cases of hepatitis B,50 cases of posthepatitic cirrhosis and 58 cases of HCC were selected and detected GP73,AFP-L3 and TGFβ1.The detection results were compared among 4 groups.Results (1)The levels of GP73,AFP-L3 and TGFβ1 had statistical differences among 4 groups(P <0.05 );(2 )The positive rate of the 3-marker combined detection reached 96.55%,which was higher than that of the 2-marker combined detection and the single marker detection.Conclusion (1) GP73,AFP-L3 and TGFβ1 may be used as the serum indicators for the early diagnosis of HCC;(2)Compared with the traditional HCC marker AFP,AFP-L3 has relatively high sensitivity and specificity and has the important role in the differential diagnosis of benign and malignant diseases of the liver,which is considered as a better serum marker in the HCC diagnosis;(3)The 3-indicator combined detection can significantly improve the positive detection rate of HCC and has better application value.

The distributioa of Se in Hb and the effects of Se intake and its chemical forms oil the distribution were studied. The results showed that Se in human Hb was all located in globin and divided equally in a and ? chains. However, a part of Se could fall from Hb during the process of isolation when more Se as SeMet was taken and "excess" Se appeared in Hb. It was implicated that there were at least two kinds of linkage between Se and Hb --tight and loose.Regarding the components of amino acid in ? and ? chains, we assumed that a tight linkage between Se and Hb was in the form of SeMet, which was incorporated into Hb by dietary Se during the synthesis of Hb; the loose linkage was formed by a part of "excess" Se and CyS, especially No.93 CyS in ? chain, being unstable sclcnotrisulf ide or selenopersulfide, which broke easily by reduction during isolation. They might be utilized first by human body when Se was required.

Resident doctors participating in clinical teaching is a disputable topic.We analyze the current situation and difficulties in clinical teaching and propose the concept of RATS(residents as teachers) program in china aiming to strengthen the training of clinical teaching skills in residents and improving culture quality of resident doctors.And we also suggest the establishment of "clinical teaching data warehouses for resident doctors"to keep pace with requirements of our resident training program in new times.

Objective To evaluate preventive effect of polysaccharides from Lycium ruthenicum Murray against photodamage in HaCaT cells,and to explore its possible mechanism.Methods Ultrasoundassisted extraction was used to extract polysaccharides from Lycium ruthenicum Murray in Qaidam Basin.In vitro cultured HaCaT cells were randomly divided into 3 groups:control group receiving no treatment,ultraviolet B (UVB) group irradiated with 30 nJ/cm2 UVB alone for 1 hour,experimental group pretreated with 2 g/L Lyeium ruthenicum polysaccharide solution followed 6 hours later by 30 mJ/cm2 UVB radiation for 1 hour.At twelve hours after UVB radiation,an inverted microscope was used to observe cell morphology.Then,MTS assay was performed to estimate cell proliferation,flow cytometry to detect cell apoptosis,an enzyme-labeled antigen method to detect levels of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px),as well as to evaluate the activity of superoxide dismutase (SOD) and catalase (CAT),and enzyme-linked immunosorbent assay (ELISA) to measure levels of intedeukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-α) in HaCaT cells and their culture supernatant.Results Compared with the control group,the UVB group showed obscure cell morphology,cell death and floating phenomenon,while cells became swollen but renained morphologically distinct in the experimental group.MTS assay revealed that the cell proliferative activity significantly differed among the above 3 groups (F =48.88,P ＜ 0.01),and the cell proliferative activity was significantly lower in the UVB group (1.72 ± 0.12) than in the control group (2.34 ± 0.11,P ＜ 0.05) and experimental group (2.11 ± 0.10,P ＜ 0.05).Moreover,the apoptosis rate was significantly higher in the UVB group (82.41％ ± 2.49％) than in the control group (3.98％ ± 0.19％,P ＜ 0.05) and experimental group (22.79％ ± 0.97％,P ＜ 0.05),as well as higher in the experimental group than in the control group (P ＜ 0.05).Compared with the control group,the UVB group showed significantly higher levels of MDA,supernatant levels of IL-1 and TNF-α,and intracellular levels of TNF-α,but significantly lower GSH-Px levels and activity of SOl and CAT (all P ＜ 0.05).However,there was no signifi-cant difference in the intracellular level of TNF-α between the UVB group and control group (P ＞ 0.05).Conclusion Lycium ruthenicum polysaccharide has protective effects against photodamage in HaCaT cells,likely by reducing the synthesis and secretion of inflammatory substances as well as free radicals.

Amyloid ; metabolism ; Humans ; Male ; Middle Aged ; Pituitary Neoplasms ; metabolism ; pathology ; surgery ; Plaque, Amyloid ; pathology ; Prolactin ; secretion ; Prolactinoma ; metabolism ; pathology ; surgery ; Synaptophysin ; metabolism

Objectives: This study was conducted to investigate the relationship between watching Mukbang (eating broadcasts) and dietary and health behavior in adults who watch Mukbang Methods: The questionnaire was administered on a self-written basis through online and offline formats to 800 adults (400 men and 400 women). The contents of the survey consisted of general characteristics, Mukbang viewing time per week, breakfast intake frequency, preference for menus when viewing Mukbang , delivery food intake frequency per week, late meal intake frequency per week, and health behavior. The subjects were divided into three groups according to Mukbang viewing time. Results: The body weight of viewers was significantly higher whenMukbang viewing time was over 14 hours for both men and women. In particular, based on the BMI (body mass index), those who watched Mukbang for more than 14 hours were found to be overweight. People with more than 14 hours of Mukbang viewing time per week were found to prefer mostly carbohydrate-rich food and meat, while those with less than 7 hours of Mukbang viewing time per week showed a higher preference for vegetables and fruits. An analysis of the frequency of breakfast eaten showed that the rate of skipping breakfast was the highest for those who watched Mukbang for more than 14 hours per week, and the rate of eating breakfast daily was the highest in the case of fewer than 7 hours of viewing. In the case of high Mukbang viewing time per week, the frequency of food delivery and night eating was high. When Mukbang viewing time was high, the viewer’s interest in health was low and the frequency of exercising too was low. Conclusions Viewers with high Mukbang viewing time showed undesirable health and eating behavior. Thus, it is believed that proper nutrition education on improving eating habits and raising the awareness of correct eating habits is necessary for such viewers.

Objectives: This study was conducted to investigate the relationship between watching Mukbang (eating broadcasts) and dietary and health behavior in adults who watch Mukbang Methods: The questionnaire was administered on a self-written basis through online and offline formats to 800 adults (400 men and 400 women). The contents of the survey consisted of general characteristics, Mukbang viewing time per week, breakfast intake frequency, preference for menus when viewing Mukbang , delivery food intake frequency per week, late meal intake frequency per week, and health behavior. The subjects were divided into three groups according to Mukbang viewing time. Results: The body weight of viewers was significantly higher whenMukbang viewing time was over 14 hours for both men and women. In particular, based on the BMI (body mass index), those who watched Mukbang for more than 14 hours were found to be overweight. People with more than 14 hours of Mukbang viewing time per week were found to prefer mostly carbohydrate-rich food and meat, while those with less than 7 hours of Mukbang viewing time per week showed a higher preference for vegetables and fruits. An analysis of the frequency of breakfast eaten showed that the rate of skipping breakfast was the highest for those who watched Mukbang for more than 14 hours per week, and the rate of eating breakfast daily was the highest in the case of fewer than 7 hours of viewing. In the case of high Mukbang viewing time per week, the frequency of food delivery and night eating was high. When Mukbang viewing time was high, the viewer’s interest in health was low and the frequency of exercising too was low. Conclusions Viewers with high Mukbang viewing time showed undesirable health and eating behavior. Thus, it is believed that proper nutrition education on improving eating habits and raising the awareness of correct eating habits is necessary for such viewers.