Objective To identify G?quadruplex structures in the promoter region of MET. Methods CD spectroscopy,UV spectroscopy,non?denatured electrophoresis and PCR stop assay were applied to indicate the G?quadruplex structure and its function. Results The Pu23WT se?quence in the promoter of MET adopted an intramolecular parallel G?quadruplex structure under physiological conditions in vitro,which can stop the extension of Pmet. Conclusion G?quadruplex structure in the promoter might inhibit MET gene expression in vivo.

Objective To study the mechanism of anti-inflammatory effects of licorice flavonoids(LF) isolated from the roots of Glycyrrhiza uralensis(licorice) on lipopolysaccharide(LPS)-induced lung inflammation in mice.Methods Mice were intratracheally instillated with either LPS(4 mg/kg) or saline.At 6 or 24 h after LPS intratracheal instillation,pathological examination and bronchoalveolar lavage were performed and the lung wet/dry weight ratio as an index of acute lung injury was assessed.Then,the numbers of total leukocytes,neutrophils and the activity of superoxide dismutase(SOD) and TNF-? protein in bronchoalveolar lavage fluid(BALF) were measured.LPS-induced myeloporoxidase(MPO) activity and expressions of TNF-? and IL-1? at the mRNA levels and TNF-? at the protein level in lung tissues were determined by RT-PCR and ELISA.Results LPS caused a severe lung inflammation,as indicated by the pathological findings and the lung wet/dry weight ratio.However,oral LF could attenuate these LPS-induced abnormalities.LF could decrease the numbers of total leukocyte cells and neutrophils,and increase the levels of SOD and TNF? BALF.In addition,LF significantly suppressed the MPO activity,TNF-? and IL-1? mRNA expression levels,and TNF-? protein level in the lung tissues.Conclusion Inhibition of inflammatory cytokines expressions and regulation of oxidation/antioxidation of LF may be its anti-inflammatory mechanism in LPS-induced lung inflammation in mice.

Objective To explore the clinical and MDCT features of pediatric irreducible intussusception. Methods 66 patients were divided into irreducible intussusception group (19 cases) and reducible intussusception group (47 cases). Age clinical courses, length of intussusception body (L), neck max diameter (D1), head max diameter (D2) andthe ratio (D2/D1) and MDCT imaging data were compared and analyzed. Results (1) The course time, L and D2/D1 values of irreducible intussusception group were significantly higher than those of reducible group, the D1 was lower than that ofreducible group, and the difference is statistically significant (P<0.05). Clinical course,L and D2/D1 value AUC values were more than 0.7, the threshold values were 33.0 h, 7.5 cm and 1.33. (2) The occurrence rate of non-ileum-colon intussusception, Meckel's diverticulum, appendicitis and intestinal necrotic for irreducible intussusception were 36.8%, 21.1%, 21.1%, 15.8%and 10.5%respectively. Conclusion Whenthe time of course>33.0 mo, D2/D1>1.33 and L>7.5 cm, the irreducible intussusceptioncould be considered, and Meckel??s diverticulum, intestinal necrosis, appendicitis and intestinal obstruction should be judged further.

Adult ; Aspergillosis ; diagnosis ; etiology ; Diagnostic Errors ; Humans ; Lung Diseases, Fungal ; diagnosis ; etiology ; Male ; Silicosis ; diagnosis ; microbiology ; Silicotuberculosis ; diagnosis ; etiology ; Tuberculosis, Pulmonary ; diagnosis ; etiology

AIM: To investigate influence of biomimetic nanoapatite coatings of titanium surface on behavior of osteoblasts-like and provide evidence for surface modification and biological effects of titanium implant.METHODS: Biomimetic nanoapatite coatings were developed by functionally modified methods with a combination of topographic,chemical and biomimetic treatments on the surface of titanium(Ti) substrate.The biological behavior and bioactivity of functionally modified SLA implants with chemical and biomimetic treatments(SCB-treated Ti) were investigated to compare with untreated Ti and SLA Ti plates as controls.The cell attachment,proliferation,alkaline phosphotase(ALP) activity,cell morphology and differentiation were evaluated by using MTT,RT-PCR,scanning electron microscopy(SEM) and confocal laser-scanning microscope(CLSM) analysis system.RESULTS: The cell adhesion and proliferation were enhanced on functionalized titanium surface with nano-scale apatite compared to the controls.SEM micrographs also revealed that the osteoblast-like cells spreadly grew along the surface.Cell morphology and differentiation were further observed distinctly by CLSM graphs.Moreover,mRNA expression of alkaline phosphotase on the SCBtreated Ti increased obviously on the twelfth day compared with the controls.CONCLUSION: The in vitro results demonstrate the remarkable improvement on cell adhesion and proliferation of the biomimetic nanoapatite on SCB-treated Ti,which could improve early bone-implant interface bonding ability and be used for orthopaedic/dental implants.

ObjectiveTo evaluate partial inferior vena cava (IVC) interruption and filter placement in the prevention of pulmonary embolism caused by lower extremity deep vein thrombosis (LEDVT).MethodsAmong 44 cases of LEDVT, 10 cases underwent partial IVC interruption, 34 cases received IVC filter placement.Results7 out of 10 cases undergoing IVC occlusion experienced post operative relief of non-fatal pulmonary embolism symptoms with no perioperative mortality or severe complications.On follow-up of 8 months, 3 cases experienced non-lethal pulmonary embolism. 24 out of 34 filter placement cases had postoperative relief of pulmonary embolism symptoms with no perioperative mortality. 2 cases suffered from inhospital pulmonary embolism with one death. 20 cases were followed up for an average of 10 months without recurrence of pulmonary embolism. Conclusion Both partial IVC interruption and filter placement are safe and effective surgical procedures for the prevention of recurrence of pulmonary embolism. The result of filter placement is more favourable.

Objective To investigate the significance of changes of esophageal peristalsis in hiatal hernia patients. Methods 32 patients with symptom of gastroesophageal reflux(esophageal hernia 10, hiatal hernia with esophagitis 12 and esophagitis 10) and 9 patients without symptom of reflux were selected.Lower esophageal sphincter (LES) pressure ,esophageal peristaltic velocity ,amplitude and duration were detected respectively;the degree of esophagitis was identified with gastroscopy. Results LES pressures in hiatal hernia with esophagitis group and simple esophagitis group were significantly lower than those in control group , there was no significant difference between hiatal hernia group and control group . In hiatal hernia group ,the peristaltic amplitudes of proximal and distal esophagus (51.3?5.4 mmHg and 83.6?8.3 mmHg) were significantly higher than those in the other three groups , while esophagitis group was significantly the lowest . There were no significant difference about esophageal peristltic duration both of proxmial and distal esophagus between these four groups .Furthermore , distal esophageal peristaltic velocity was significantly higher in hiatal hernia group than that in the other three groups , and it was the lowest in esophagitis group .Conclusion The changes of esophageal peristalsis in patient with hiatal hernia play an important role in the pathogenesis of gastroesophageal reflux and mucosal injury.[

Antibody bridging keratinocyte/mononuclear leucocyte adhesion(ABKMA), an experimental model in vitro for psoriasis, has been used in the investigation of cytokine network changes. ABKMA was formed by using normal human epidermal cells incubated with IgG of patients with psoriasis, and then mononuclear leucocytes(ML) of patients with psoriasis were added. Interleukin 2 and interleukin 6 in culture supernate were estimated by ABC amplified ELISA. The results indicated that the synthesis and secretion of interleukin 2 and interleukin 6 were higher than normal IgG and serum free medium controls. Immunohistochemistry staining was used to demonstrate the cell surface interleukin 2 receptor ? chain (IL2R?). The expression of IL2R? +ML/ML and IL2R? +ML/adhesion ML was 4.6% and 40.4% respectively. Both these data were higher than corresponding controls.

BACKGROUND:Host immune rejection is the main problem for nerve alograft in the repair of nerve defects. Therefore, how to avoid and minimize the immune rejection is the key to the success of nerve alografting. OBJECTIVE: To develop a new nerve pretreatment method by which Schwann cels and myelin can be removed from the peripheral nerve of dogs while the basilar membrane can be reserved integraly in order to obtain acelular nerve alografts. METHODS:Bilateral sciatic nerves from healthy adult dogs were taken and pretreated with the combined freeze-thaw and chemical methods folowed by microscopic observation of ultrastructural features, histological staining and western blot analysis of its ingredients. RESULTS AND CONCLUSION:Pretreated acelular nerves with good ductility and excelent epineurium toughness were empty basal lamina tubes with no Schwann cels, myelin and fragments that were al removed thoroughly, but the basilar membrane was fuly retained. These findings indicate that the optimized combination of freeze-thaw and chemical methods can efficiently clear Schwann cels and myelin which are the major antigenic components in the peripheral nerve, while preserve the basilar membrane to promote nerve regeneration. Therefore, this method can be an ideal method for preparation of tissue engineered nerves.

Objective To investigate the differential gene expression in chronic hepatitis B (CHB) patients with typical syndromes of traditional Chinese medicine ( TCM) syndromes, and to explore the relationship between TCM syndromes and gene expression. Methods Peripheral blood samples were collected from CHB patients and healthy volunteers before treatment. After total RNA of leukocytes was isolated, the gene expression profiles were detected by microarray. The expression levels of partial genes were tested by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) . Results Microarray analysis results showed that there were significant differences of gene expression between CHB patients and healthy volunteers, and among CHB patients with the syndrome of liver-qi stagnation and spleen deficiency, syndrome of damp-heat accumulation, and syndrome of liver-kidney yin deficiency. The results of gene ontology ( GO) and signal pathway analysis between the healthy control and CHB patients with various syndromes showed that the specially-regulated genes of CHB patients with liver-qi stagnation and spleen deficiency were mainly related to cytokinetic process, those in patients with dampness-heat accumulation were mainly related to the positive regulation of lipid storage, and those of patients with liver-kidney yin deficiency were mainly related to the activities of nitric oxide synthase regulator. The real time RT-PCR for partial genes presented the similar results with those of gene microarray. Conclusion There are specific expression profiles of differential genes and significant differential genes in CHB patients with the syndrome of liver-qi stagnation and spleen deficiency, syndrome of damp-heat accumulation, and syndrome of liver-kidney yin deficiency, which may be the molecular foundation for the classification of TCM syndromes of CHB patients.