1.Investigation of relationship between schizophrenia and ten kinds of elements in serum
Lailai YAN ; Chao LV ; Jinyun QIU ; Yaqiong LIU ; Jingyu WANG
Chinese Journal of Biochemical Pharmaceutics 2015;(6):55-58
Objective To study the relationship between the changes of ten elemental contents in serum and schizophrenia.Methods Calcium (Ca), magnesium (Mg), zinc (Zn), iron (Fe), aluminum (Al), arsenic (As), selenium (Se), copper (Cu), lead (Pb), manganese (Mn) in serum in the of schizophrenic patients and normal subjects are measured by inductively coupled plasma mass spectrometer and inductively coupled plasma emission spectrometer, compared the content of elements between the schizophrenia and normal.Results There were significant differences ( P<0.05) in other elements between two groups except lead.The contents of Ca,Fe,Mg,Zn,As,Se,Cu in the patients with schizophrenia were significantly lower than those in control, while Al, Mn were significantly higher than those in control.Most of elements in serum between case and control groups were correlated (P<0.05).Some of element ratios which have physiological significance were significant statistically (P<0.05), such as: Ca/Mg, Ca/Fe and so on.Conclusion These findings suggest that the patients with schizophrenia might have an abnormal elemental metabolism.Given the relationship among the elements, the element ratios are more valuable in reflecting the relationship between schizophrenia and elements.
2.The effects of mild hypothermia on the expression of myeloperoxidase and cyclooxygenase 2 in rats after cerebral ischemia and reperfusion
Zhi-Chao LIU ; Cheng-Yan LI ; Qiu-Xiang CHENG ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(12):-
Objective To investigate the effects of mild hypothermia ( MH ) on the expression of my- eloperoxidase(MPO) and cyclooxygenase 2 (COX 2) in rats after cerebral ischemia and reperfusion (CIR). Methods Forty-eight Wistar rats were randomly divided into four control groups (n=6 in each) and four MH groups (n=6 in each).CIR models were established by suture occlusion of the left middle cerebral artery.The rats in the MH groups,but not in the control groups,were treated with MH.Rats were killed at 4 h,8 h,12 h and 16 h after CIR.MPO expression was measured,along with the expression of COX 2 as measured by Western blot- ting and immunohistochemical methods.Results Compared with the control groups,MPO activity and the COX 2 expression in the cortex and striatum were significantly lower in all the MH groups at 4 h,8 h,12 h and 16 h after CIR.Conclusion MH treatment can protect neurons by decreasing MPO activity and COX 2 expression,allevia- ting inflammation and reducing secondary injuries after CIR.
3.A new research paradigm in modernization of traditional Chinese medicine: single cell pharmacology
Jia-yun CHEN ; Qiu-yan GUO ; Cheng-chao XU ; Ji-gang WANG
Acta Pharmaceutica Sinica 2021;56(12):3300-3312
Single cell "omics" technology enables the capture of genome, transcriptome, proteome and other omics information in a high-throughput and unbiased manner at single-cell resolution, allowing the characterization of the functional state of individual cells to reveal their heterogeneity and differential responses to drug treatment. This technology has wide application in pharmacological research, facilitating drug screening, efficacy evaluation, and mechanistic studies. We envision that, in the field of traditional Chinese medicine (TCM), single cell omics technology can be applied in the identification of active ingredients and drug targets, and elucidation of drug mechanism of action. In this article, we briefly introduce the single cell omics technology - particularly single cell transcriptome sequencing, and review its application in the field of modern drug research. Based on that, we propose the concept of "single cell pharmacology" and articulate how it can be applied to transform the pharmacological research of TCM and promote TCM modernization.
4.Murine model of Graves disease induced by thyroid-stimulating hormone receptor gene transfected via liposome.
Wen LONG ; Li-heng QIU ; Chao MENG ; Yan-song LIN
Acta Academiae Medicinae Sinicae 2012;34(6):550-555
OBJECTIVETo compare the efficacy of different expression vectors, target genes, and immunization procedures in transfecting mice via liposome to construct murine model of Graves disease.
METHODSWe linked pCDNA3.1(+) and pUBC to full-length human TSHR and TSHR A subunit cDNA to yield four plasmids, which were later injected intramascularly or subcutaneously into female Balb/c mice via liposome. The blood anti-TSHR antibody (TRAb) were determined and the body weight were measured after each immunization. Serum thyroid hormone levels were measured after the animals were sacrificed.
RESULTSIn mice immunized with pUBC, no significant variance with control in weight nor serum TRAb concentration was observed. Weight gain in pCDNA3.1(+) group was significantlyly slower than controls (p<0.05), and serum TRAb concentration was also significantly elevated. In pCDNA group, animals immunized with TSHR A subunit (TSHRA subgroup) as the target gene revealed even significantly slower weight gain (p<0.001) and even faster TRAb elevation than those immunized with full length TSHR. Significantly higher FT4 (p=0.023) was observed in TSHRA and TSHR subgroups, which was reversely correlated to weight gain, but no significant difference (p>0.05) in FT3 was observed. Weight gain and TRAb concentration mainly varied in the later period of immunization.
CONCLUSIONSImmunization with pCDNA3.1(+) and TSHR A subunit gene together with higher immunization frequency increases the chance of model induction. Furthermore, FT4 is a better indicator for assessing the thyroid function in this model.
Animals ; Disease Models, Animal ; Female ; Graves Disease ; genetics ; Liposomes ; Mice ; Mice, Inbred BALB C ; Receptors, Thyrotropin ; genetics ; Transfection
5.HIV-1 infection changes miRNA expression profile in the whole blood.
Ling-Yan ZHU ; Chao QIU ; Jian-Xin LV ; Jian-Qing XU
Chinese Journal of Virology 2013;29(3):323-329
To describe the unique miRNA profiles for HIV seropositive individuals and identify significantly differently expressed miRNAs, we determined the expression level of 754 miRNAs of 10 HIV seropositive individuals and 10 HIV seronegative individuals by using the Taqman low density microRNA array. BRB-Array Tool was used to conduct the significance analysis, and the DIANA online tool was used to perform the miRNA target prediction and pathway analysis. A total of 56 significantly differentially expressed miRNAs were identified by microarray between HIV seropositive and seronegative individuals. Among them, 49 miRNAs were down-regulated and 7 were up-regulated, partially overlapped with reported data. Predicted target genes were mainly involved in MAPK, TGF-beta and Wnt pathways. The results shows that miRNA profile changes in HIV-1 seropositive individuals, and the 56 differentially expressed miRNAs may play important role during HIV infection. Further studies on these miRNAs may be helpful for identify key molecules involved in HIV infection and potential diagnostic markers.
Adult
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Female
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Gene Expression Profiling
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HIV Infections
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blood
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genetics
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virology
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HIV-1
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genetics
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physiology
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Humans
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Male
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MicroRNAs
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blood
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genetics
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Young Adult
6.Change in plasma ghrelin level and the relation between ghrelin and insulin resistance in type 2 diabetic patients after rosiglitazone therapy
Yan-Ming GAO ; Gui-Zhi LU ; Qiu-Ming JIANG ; Ai-Mei DONG ; Xiao-Hui GUO ; Yan GAO ; Yong-Zheng PANG ; Chao-shu TANG ;
Chinese Journal of Endocrinology and Metabolism 1985;0(02):-
The change in plasma ghrelin level after 4-and 12-week adjunctive therapy of rosiglitazones in type 2 diabetic patients inadequately controlled by sulphonylurea alone was observed and the relation between ghrelin and insulin resistance was analysed.The results showed that rosiglitazones significantly increased circulating ghrelin level and obviously decreased insulin resistance index after therapy for 4 and 12 weeks in type 2 diabetic patients.
7.Correlation between the expression of JARID1B/KDM5B in breast cancer tissues and the circulating tumor cells
Li-Hua ZHAO ; Jia WEI ; Ying-Li HUAI ; Xiao-Jing ZHANG ; Chao ZHANG ; Qiu-Lan LIN ; Ke-Jie WANG ; Hong-Yan YAN ; Jia-Liang GUO ; Qiu-Ying LI
Basic & Clinical Medicine 2018;38(4):512-515
Objective To investigate the relation between expression of JARID1B/KDM5B in invasive breast cancer tissue and circulating tumor cells of invasive breast patients. Methods The S-P immunohistochemical method was used to observe the expression of JARID1B/KDM5B in lesions.imFISH method was used to detect circulation tumor cells. Results Twenty-seven cases of patients showed CTC positive(the number of CTC was more than or equal to 2) in 35 invasive breast cancer cases,The results of JARID1B/KDM5B expression were as follows:(-) (n=0) and (+) in 1 case,(++) in 3 cases,(+++) in 23 cases,8 cases of negative cases (the number of CTC < 2), JARID1B/KDM5B immune group of results:(-) (n=2),(+) in 2 cases,(++) in 1 case,(+++) in 3 cases, The positive relation was found in expression of JARID1B/KDM5B and circulating tumor cells(P<0.05). Conclu-sions JARID1B/KDM5B positive expression in tumor tissues has a certain significance in predicting the recurrence and metastasis of tumor.
8.Chemical constituents from Perovskia atriplicifolia.
Jun ZHONG ; Chao-guan HUANG ; Yi-Jiang YU ; Zhong-qiu LI ; Wei WANG ; Xiang-zhong HUANG ; Wen-xing LIU ; Yan YUAN ; Zhi-yong JIANG
China Journal of Chinese Materia Medica 2015;40(6):1108-1113
An investigation on the chemical constituents of the 90% EtOH extract of Perovskia atriplicifolia led to the isolation of fifteen compounds from the EtOAc fraction. Based on the detailed spectral analysis (MS, 1D and 2D NMR), as well as comparison with the literatures, the structures of compounds 1-15 were determined as cirsimaritin (1), salvigenin (2), syringaldehyde (3), vinyl caffeate (4), 2α, 3α-dihydroxyolean-12-en-28-oicacid (5), 2α, 3α-dihydroxyurs-12-en-28-oicacid (6), niga-ichigoside F1 (2α, 3β, 19α, 23- tetrahydroxyurs - 12-en-28-oicacid- O-β-D- glucopyranoside, 7), sericoside (8), 4-epi-niga-ichigoside F1 (2α, 3β, 19α, 24-tetrahydroxyurs-12-en-28-oicacid O-β-D-glucopyranoside, 9), 2α, 3β, 24-trihydroxyolean-12-en-28-oicacid O-β-D-glucopyranosyl-(1 --> 2) - β-D-glucopyranoside (10), pruvuloside A (11), asteryunnanoside A [2α, 3β, 23-trihydroxyolean-12-en-28-oicacid O-β-D-glucopyranosyl-(1 --> 2)-β- D- glucopyranoside,12], rosmarinic acid methyl ester (13), β-sitosterol (14), and daucosterol (15), respectively. Compounds 1-13 were isolated from the Perovskia genus for the first time. All the compounds were obtained from P. atriplicifolia for the first time.
Drugs, Chinese Herbal
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chemistry
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isolation & purification
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Lamiaceae
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chemistry
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Spectrometry, Mass, Electrospray Ionization
9.Research progress of the anti-HIV activity of SAMHD1.
Jun HUANG ; Chao QIU ; Xiao-Yan ZHANG
Chinese Journal of Virology 2012;28(4):477-481
The SAM and HD domain containing protein 1 (Sterile alpha motif domain and HD domain-containing protein 1, SAMHD1) is a putative negative regulator of the antiviral innate immune response. It can significantly increase the antiviral immune response, mediates the interferon-induced inflammatory response involved in the host foreign-virus defense system. The early studies have focused on its gene mutations associated with Aicardi-Goutières syndrome (AGS), the latest study found that SAMHD1 as a potent dGTP-stimulated triphosphohydrolase restricts HIV-1 replication by hydrolyzing the majority of cellular dNTPs, thus inhibiting reverse transcription and viral complementary DNA (cDNA) synthesis. Auxiliary gene of HIV-2 and simian immunodeficiency virus (SIVsm / mac) encoding the Vpx protein can eliminate HIV-1 restriction. In recent years, the research on SAMHD1, mores forward rapidly this paper overviews the recent research progression related to the above fields.
Animals
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Cell Line
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HIV
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metabolism
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physiology
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Humans
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Monomeric GTP-Binding Proteins
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genetics
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metabolism
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SAM Domain and HD Domain-Containing Protein 1
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Viral Regulatory and Accessory Proteins
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metabolism
10.Anaplastic myeloma and T-cell lymphoma in the same patient.
Dao-zi JIANG ; Chao-hua DENG ; Yan-han JIA ; Li ZHANG ; Ze-sheng WANG ; Shang-qin LIU ; Qing-ping GAO ; Qiu-ping ZHANG
Chinese Medical Journal 2013;126(5):996-996