2.Research development of APC gene promoter methylation in gynecological cancer
Cancer Research and Clinic 2011;23(6):428-430
Adenomatous polyposis coli gene is an important anti-oncogene of the Wnt signaling pathway. The promoter hypermethylation of APC gene can effect the expression of APC mRNA and APC proteinum, which can make Wnt signaling pathway anomalous. Recently. the promoter hypermethylation of APC Gene in gynecological tumors is found in many studies, and it is related to the aggravation level of tumor.This paper reviews the influencing factors and progress of promoter methylation of APC gene in Gynecological cancer.
3.The development of military acute stress scale
Chinese Journal of Behavioral Medicine and Brain Science 2012;21(7):658-661
ObjectiveTo develop the military acute stress scale and examine the reliability and validity of this scale.MethodsBased upon the previous acute stress researches and the rule of psychometrics,the military acute stress scale was compiled according to open-ended inventory survey.A total of 630 personnel were chosen by random cluster sampling,and tested with this scale,50 of them were tested by Chinese Military psychosomatic health Scale simultaneously,and 50 of them were retested after two week.The reliability and validity were examined by exploratory factor analysis,confirmatory factor analysis and correlation analysis.ResultsThe military acute stress scale was comprised of 9 factors and one lie sub-scale ; Main fit indexes of model were the following CMIN =1219.476,DF =593,P =0.000,CMIN/DF =2.056,IFI =0.927,TLI =0.902,CFI =0.924,RMSEA =0.059; The conrelation coefficients between subscales and subscales were 0.284-0.572.The correlation coefficients between subscales and total scale were 0.700 ~ 0.846 (P < 0.05 or 0.01 ) ; The correlation coefficients was significant between this scale and Chinese military psychosomatic health scale.The correlation coefficients were 0.338-0.962 (P < 0.01 ).The test-retest correlation coefficients of total scale and subscales were 0.379-0.909 (P < 0.01 ).The Cronbaeh's α coefficients were 0.606-0.926 ; and the split-half correlation coefficients were 0.466-0.855.ConclusionThe military acute stress scale has acceptable psychometrics properties on reliability and validity.
4.Reading therapy in different kinds of library:A comparative analysis
Chinese Journal of Medical Library and Information Science 2014;(1):45-47
Reading therapy in hospital libraries, academic libraries and public libraries was described in terms of its theoretical study and practical study,and its significance in hospital library was elaborated by comparative analysis.
5.Activator protein-1 mRNA expression, cell proliferation and collagen synthesis induced by tumor necrosis factor-alpha in cardiac fibroblasts from rats and the effect of Qiangxin Decoction
Journal of Integrative Medicine 2008;6(9):946-51
OBJECTIVE: To investigate the function of activator protein-1 (AP-1) in proliferation and collagen synthesis of cardiac fibroblasts (CFs) in neonatal rats induced by tumor necrosis factor-alpha (TNF-alpha), and to explore the mechanism of Qiangxin Decoction (QXD), a compound traditional Chinese herbal medicine, in reversing cardiac fibrosis. METHODS: CFs derived from neonatal rats were cultured with enzymatic dissociation, and fibrosis of the CFs was induced by TNF-alpha. The CFs were divided into normal control group, untreated group, 5% QXD-containing serum group, 10% QXD-containing serum group and 20% QXD-containing serum group. After 24-hour culture of QXD-containing serum, AP-1 mRNA was detected by real-time fluorescent quantitative polymerase chain reaction; proliferation and collagen synthesis of CFs were assayed by thiazolyl blue assay (MTT) and measured by hydroxyproline respectively, in order to determine the effect of different dosage decoction on the proliferation and collagen synthesis of CFs. RESULTS: After 24-hour stimulation of TNF-alpha in CFs, compared with those in normal control group, the expression of AP-1 mRNA and cardiac fibroblast proliferation and collagen synthesis increased significantly (P<0.05). However, Qiangxin Decoction could reduce the expression of AP-1 mRNA and decreased the proliferation and collagen synthesis of CFs significantly (P<0.05). CONCLUSION: Qiangxin Decoction can inhibit proliferation and collagen synthesis of CFs induced by TNF-alpha, and reverse cardiac fibrosis, which may be related to its down-regulation of the expression of AP-1.
6.A meta-analysis on the efficacy and safety of autologous drainage blood reinfusion in total knee arthroplasty
The Journal of Practical Medicine 2014;(10):1644-1647
Objective To assess the efficacy and safety of autologous drainage blood reinfusion in total knee arthroplasty(TKA). Methods Computers were used to search the Cochrane Central Register of Controlled Trials, MEDLINE, EMBASE, and VIP. The randomized controlled trials (RCTs) of autologous drainage blood reinfusion and vacuum suction without reinfusion after TKA were collected. And the results were analyzed by using the software RevMan5.1. Results Eight RCTs met the criteria for meta-analysis. Use of autologous drainage blood reinfusion significantly reduced the average amount of units of homologous blood transfusion, the length of hospital stay, and the number of patients requiring homologous blood transfusion. The rates of DVT formation and incision infection did not differ significantly between the two groups. Conclusions Autologous drainage blood reinfusion is a safe, effective procedure with less blood transfusion and medical cost. It can lower volume of allogeneic blood transfusion , shorten length of hospital stay, and avoid occurrence of complications.
7.Study of the effect of recombinant growth hormone on the tumor cell proliferation in the transplantation liver tumor models in rats
Chinese Journal of General Surgery 1993;0(03):-
Objective To study the effect of reconbinant growrh hormone(rGH) on the tumor cell proliferation in the transplantation liver tumor models in rats,to explore the advantages and disadvantages of rGH in the treatment of liver carcinoma.Methods The male weaning rats were used to vaccinate and transgenerate by intraperitoneal injection of abdominal fluid contarning tumor cells from the celiar transplantation tumor rats, the others weaning rats were used to be the solid tumor rats by subcutaneous vaccination with abdominal fluid of tumor cells.The solid tumor were divided into pieces (2 mm?2 mm?3 mm),which were inserted into the liver of the 82 adult male rats to establish the transplantation liver tumor models.and then the models were divided into experimental group and control group randomly.After 3 days,experimental group were given rGH (0.5U/ kg?d)and control group were given via caudal vein NS for 5 days.All the rats were killed in 8d,10d,12d,respectively.The rats, tumors and livers were weighted,the number of hepatocytes,carcinoma cells and mitotic index were measured,the number of 3 H-TdR incorporation in vivo was measured in 8 days after operation. Results There were no significant difference in all the results between the two groups,except the rat's weight of 8days after operation and the tumor weight in different phase.Conclusions The rGH has no significant effect on the proliferation of tumor cell in transplantation liver models in rats.
8.Clinical laboratory tests of venous thromboembolism in obstetrics and gynecology
Chinese Journal of Laboratory Medicine 2017;40(3):149-151
Venous thrombotic disease is a serious disease,which impact on health and life-threatening.Pulmonary embolism and deep-vein thrombosis are the two components of a single disease called venous thromboembolism in obstetrics and gynecology.but it can be difficult to diagnose because clinical symptoms and signs are non-specific or absent in early venous thrombus embolism (VTE).It has great value that how to use the most economic,simple,efficient method for screened high-risk groups,timely and accurate laboratory diagnosis of VTE.
9.Clinical features and ultrasound characteristics of benign and malignant thyroid nodules
Chinese Journal of General Practitioners 2017;16(4):304-306
The clinical data and ultrasound findings of 185 patients with thyroid nodules undergoing thyroidectomy from June to December 2015 were retrospectively reviewed.Postoperative pathology confirmed malignant thyroid nodules in 86 cases and benign nodules in 99 cases.The ultrasonographic findings showed that there were significant differences in obscure boundary [51% (44/86) vs.16% (16/99)],hypoecho [(95% (82/86) vs.27% (27/99)],internal micro-calcification [63% (54/86) vs.21% (21/99)] and lymph node enlargement [33% (28/86) vs.5% (5/99)] between malignant and benign nodules (all P < 0.05).The sensitivity,specificity,positive predictive value,negative predictive value and accuracy of thyroid imaging reporting and data system (TI-RADS) in differentiation of malignant and benign thyroid nodules were 77% (66/86),86% (85/99),83% (66/80),81% (85/105) and 82% (151/185),respectively.The positive rates of thyroid globulin antibody(TGAb) and thyroid peroxydase antibody (TPO-Ab) in malignant nodules [6% (5/79) and 25% (20/79)] were significantly higher than those in benign nodules [0 (0/94) and 12% (11/94)] (both P <0.05).There was no significant difference in free T3 (FT3),free T4 (FT4) and TSH between the two groups [(4.91-± 0.78) vs.(4.93-± 1.98) pmol/L,(13.57 ±2.22) vs.(13.58 ±2.17)pmol/Land(1.78±1.40) vs.(1.88±2.36)mU/L] (allP>0.05).The results indicate that TI-RADS grading and thyroid immunity index are valuable for differential diagnosis of thyroid nodules.
10.WeChat service in hospitals directly under the Yunnan Health and Family Planning Commission
Chinese Journal of Medical Library and Information Science 2016;25(3):73-75
The WeChat service provided at present in 14 hospitals directly under the Yunnan Health and Family Planning Commission was surveyed with suggestions proposed for providing active Wechat service in hospitals in order to alleviate the difficulty to see a doctor and the tense relationship between doctors and patients.