3.Atypical extraventricular neurocytoma: report of a case.
Yan LI ; Jing FU ; Yanke GUO ; Zhichao WANG
Chinese Journal of Pathology 2014;43(11):774-775
4.Plasmacytoid-like oncocytic carcinoid of lung: report of a case.
Yongliang TENG ; Yabin ZOU ; Xu YAN ; Dianbo CAO ; Liang GUO
Chinese Journal of Pathology 2015;44(5):344-345
6.Primary intracranial mxyoid liposarcoma: report of a case.
Liang GUO ; Dian-bo CAO ; Xu YAN ; Ya-bin ZOU ; Hong-xi MA
Chinese Journal of Pathology 2013;42(12):843-844
7.Application of negative pressure attraction method of brushing and washing in oral care of patients with orotracheal intubation
E FENG ; Yan-Ling YIN ; Xin-Rong GUO ; Hui-Ling ZHENG
Chinese Journal of Modern Nursing 2012;18(11):1280-1282
Objective To explore the effect of negative pressure attraction method of brushing and washing apply in oral care of patients with orotracheal intubation.Methods Totals of 82 oral intubation patients in ICU were randomly selected and divided into experience group ( n =42 ) and control group ( n =40 ).The traditional method of oral care was used in control group,while the negative pressure attraction method of brushing and washing was used in experience group,then,the incidence of oral infections,ventilator-associated pneumonia( VAP),dental plaque and oral mucosal injury were observed in two groups.Results The incidence of oral infections,ventilator-associated pneumonia(VAP),dental plaque in experience group respectively were 7.14%,2.38%,4.76%,and that in control group were 22.50%,15.00%,32.50%,the differences were statistically significant ( x2 =3.88,4.18,10.55,respectively; P < 0.05 ),while there was no significant difference in the incidence of oral mucosal injury between two groups ( P > 0.05 ).Conclusions The negative pressure attraction method of brushing and washing can effectively keep the oral clean of patients with orotracheal intubation,reduce the incidence of oral infections and ventilator-associated pneumonia and dental plaque,when compared with the traditional method of oral care.
8.Construction and identification of the two lentiviral eukaryotic expression vectors of pEGFP-N1-HIF-lα and pEGFP-N1-muHIF-1α
Shu-Hong WANG ; Xiong ZHANG ; Jin-E ZHANG ; Hua-Yan GUO ; Na GUO ; Yuan-Liang HUANG
Chinese Journal of Stomatology 2012;47(z1):244-247
Objective To construct two lentiviral eukaryotic expression vectors of pEGFP-N1-HIF-1α and pEGFP-N1-muHIF-1α and identificate the gene sequences.Methods Primers were designed according to wild-type human HIF-1 α gene sequence information and determined restriction sites of human HIF-1α point mutant sequence,taking human HIF-1α and constructed HIF-1α gene mutant clone as a template for polymerase chain reaction(PCR) amplification.Then,the target gene PCR product and purpose vector were digested.Adapter-ligated fragments of the target fragment with purpose vector were transformed into DH-5α competent cells using the recombinant PCR methods.Finally,the positive clones were selected and sequenced to verify whether the sequence of insert fragments in recombinant clones was consistent with oligo sequences designed or not.Results Through recombinant clone colony PCR,enzyme digestion of the recombinant clones and plasmid cloning and sequencing,the fragment sequences inserted in recombinant clones were consistent with the designed oligo sequences.Conclusions The two lentiviral eukaryotic expression vectors of pEGFP-N1-HIF-1α and pEGFP-NI-muHIF-1α were successfully constructed and applicable for follow-up experiments on transfection of bone marrow mesenchymal stem cells.
9.Nemaline myopathy: report of a case.
Hong-ran WU ; Xing LIU ; Li-yan SUN ; Yi BU ; Yan-su GUO ; Dong-xia WU ; Xue-qin SONG
Chinese Journal of Pathology 2013;42(6):407-408
10.Effect of intrathecal ketamine injection on protein kinase C expression in the spinal dorsal horn of rats with formalin-induced pain.
Yong YANG ; Qu-lian GUO ; Wang-yuan ZOU ; E WANG ; Jian-qin YAN
Journal of Southern Medical University 2011;31(3):461-464
OBJECTIVETo investigate the expression of protein kinase C (PKC) in the spinal dorsal horn of rats with formalin-induced pain and the effect of intrathecal ketamine on PKC expression.
METHODSThirty-two SD rats were randomly divided into 4 equal groups, namely the control group, intrathecal saline group (NS), 50 µg ketamine group (K1) and 100 µg ketamine group (K2). The rats were anesthetized with 10% chloral hydrate, and a microspinal catheter was inserted intrathecally into the lumbar region. Five days later, the rats in groups, K1 and K2 were subjected to intrathecal administration of 50 and 100 µg ketamine (10 µl), respectively, followed by 10 µl saline, and those in NS group received 20 µl saline only. Thirty minutes later, 5% formalin (50 µl) was subcutaneously injected into the left hindpaw. The pain intensity score (PIS) was utilized to assess antinociceptive behavior within 1 h after formalin injection. Twenty-four hours later, the left hindpaw thickness was measured and the expression of PKC in the spinal dorsal horn in the L5 segment was assayed using immunohistochemistry.
RESULTSCompared to group NS, groups K1 and K2 showed significantly decreased PIS (P<0.01) in the second phase of formalin-induced pain; 24 h later, the left hindpaw thickness of group NS increased obviously in comparison with that in the control group (P<0.01), whereas the thickness was significantly reduced in group K1 and K2 as compared to that in group NS (P<0.05). The number of immunoreactive cells and the immunohistochemical score of PKC in the spinal dorsal horn were significantly higher in group NS than in group C (P<0.01), but significantly lower in groups K1 and K2 than in group NS (P<0.05).
CONCLUSIONIntrathecal ketamine produces obvious antinociception against formalin-induced pain in rats and inhibits the enhanced PKC expression in the spinal dorsal horn in response to formalin-induced pain, suggesting the important role of PKC in nociceptive signal transmission and modulation in the spinal cord.
Animals ; Formaldehyde ; adverse effects ; Injections, Spinal ; Ketamine ; administration & dosage ; pharmacology ; Male ; Pain ; chemically induced ; metabolism ; Pain Measurement ; Posterior Horn Cells ; metabolism ; Protein Kinase C ; metabolism ; Rats ; Rats, Sprague-Dawley ; Spinal Cord ; drug effects ; metabolism