[Objective] To discuss the clinical effect of nasal intestinal obstruction(IO) conduct catheterization on IO after ovarian cancer operation. [Method] Choose 25 cases of IO after ovarian cancer operation, randomly divide them into treatment group 10 cases and control one 15 cases. The control group take general nasogastric tube imbedding, the treatment group take nasal IO conduct catheterization;observe their post-operational complications and clinical cure effect. [Result] On complications in control group, 8 cases had electrolyte disturbance, with complication rate 53.33%, the part of clinical symptoms was re-lieved, the IO signs did not disappear or were reduced partly with X-ray;5 cases were cured clinical y, 3 had marked effect, 3 were effective, 4 had no effect, the total effective rate was 73.33%;for the treatment group, al cases were successful y imbedded and made decompression and drainage, there ’s no severe side effects after imbedding tube, the IO symptoms were effectively relieved;1 case was transferred to operation;6 cases were cured clinical y, 3 had marked effect, 1 was effective, 0 had no effect, the total effective rate was 100%;after cathetering, there ’s no severe complications, among which, 2 cases had electrolyte dis-turbance, the post-operation complication rate was 20%. The comparison of complication rate and clinical effective rate of both groups had difference of sta-tistical meaning. [Conclusion] Nasal IO conduct imbedding treating IO after ovarian cancer operation had more effect on relieving IO symptoms and reduc-ing complications than nasogastric tube.

Objective To investigate the expression patterns of osteopontin (OPN) , vascular endothelial growth factor-A (VEGF-A) , and hypoxia-inducible factor-1α (HIF-1α) in primary hepatocellular carcinoma (HCC) and determine the clinical significance of this differential expression profile.Methods Immunohistochemical staining of OPN, VEGF-A, and HIF-1α was carried out on primary HCC tissues from 90 patients, HCC-adjacent cirrhosis tissues from 20 of those patients, and normal liver tissues from 15 healthy controls.Correlations between expression levels and HCC clinicopathological characteristics were assessed by Spearman's correlation coefficient.Results The majority of HCC tissues showed positive immunostaining for OPN (69/90, 76.67%) , VEGF-A (64/90, 71.11%) , and HIF-1α (66/90, 73.33%) .OPN-and VEGF-A-positivity were significantly higher than the results from the cirrhosis tissues and normal tissues.HIF-1α-positivity was similar between the HCC and cirrhosis tissues, but both were significantly different from the normal tissues.The differential expressions of OPN, VEGF-A, and HIF-1α were significantly correlated with tumor thrombus, capsular integrity, tumor differentiation and stage, and metastasis (P<0.05) .Conclusion HCC tissues overexpress OPN, VEGF-A, and HIF-1α and this differential profile may be related to HCC progression.Future investigations of this triad of factors may provide novel insights into the biological characteristics of HCC and reveal important targets of molecular therapy.

Objective To establish a liquid chromatographic-mass spectrometric method ( LC-MS/MS method ) for determination of dextrorphan in human liver microsome. Methods LC-MS/MS was adopted with carbamazepine serving as an internal standard.The separation was performed on Agilent ZORBAX XDB-C18 column (2.1 mm×50 mm, 3.5μm), with mobile phase consisting of 0. 05% formic acid methanol-0. 05% formic acid in gradient elution. Dextrorphan and carbamazepine were detected on multiple reaction monitoring(MRM)mode by transitions from precursor to production(m/z 258.1→199.1, 237.1→194.1). Results The linear range of dextrorphan concentration was 19.22-768 960 ng.L-1(r=0.999 8), and the lowest quantification limit was 19.22 ng.L-1.The relative recoveries were 94.02%-98.74%, and the RSDs of intra-day and inter-day were within 10%.IC50 of psoralen on CYP2D6 was 0.6μmol.L-1. Conclusion The LC-MS/MS method is proved to be rapid, sensitive and reproducible, psoralen is a strong inhibitor of CYP2D6.

Objective To observe the effect of acupuncture on post-stroke belching. Method Ninety-six patients with post-stroke belching were randomized into a control group and a treatment group by random number table, 48 cases in each group. The control group was intervened by conventional Western medication, while the treatment group was by acupuncture in addition to the medication given to the control group. The blood Cl﹣ and Ca2﹢ contents, as well as the symptoms score and occurrence of adverse reactions were compared before and after treatment. Result After intervention, the blood Cl﹣ and Ca2﹢ contents increased and the symptoms score decreased to different extent in both groups; except for the blood Ca2﹢ content, there were significant differences in comparing all the parameters between the two groups after intervention (P<0.05); there was a significant difference in comparing the occurrence of adverse reactions between the two groups (P<0.05), and there were less adverse reactions in the treatment group. Conclusion Acupuncture can effectively control the post-stroke belching and reduce the happening of adverse reactions.

The personality androgyny is an important topic of personality development,but in the last several decades,the research mostly set out from the society,more emphasize education factor,few materialize the child corpus in the growth,few materialize the concrete meanings to children's mind and body health.So these results will be applying realm to dispute rather many,haven't materialized the value it should have.As the master of psychoanalysis,Jung's prototype theories is abundant,anima and animus,personality mask and the subconscious,formated an organic unify with consciousnes.These theories have an important significance to re-examine the content and value of children's personality bisexual in the theories field.The personality bisexual strategy according to Jung's theories will be more ethical because of respecting children's corpus and consideration children's physical and mental health.

This study was carried out to determine the effects of mild iron deficiency anemia (IDA) on physical work capacity (PWC) of 7-year-old children in Beijing. Ninety-three mild IDA children and thirty-seven well-to-do children served as volunteer subjects. Finger blood samples were obtained for Hb, FEP, FEP/Hb, SF determination; PWC data were assessed by measuring PWC 170, R.PWC170, Workload Heart Rates (W.HR), estimated Vo2max before, in the middle, and after treatment, respectively. After the mild IDA children were cured with iron supplement (300mg ferrous sulfate per day) for 60 days, their values of Hb, FEP, FEP/Hb, SF changed significantly from original 10.5g/dl, 41.8ng/dl, 4.6?g/g, 13.2ng/ml to 12.5g/dl, 24.1?g/dl, 2.0?g/g, 75.7ng/ml respectively (P

Thirty two rabbits were divided into 4 groups: (1) Normal diet control group, ( 2 ) Hyperlipid diet control group, ( 3 ) Hyperlipid diet group injected with 30 mg/kg GSL, ( 4 ) Hyperlipid diet group injected with 60 mg/kg GSL, The whole expeiment took about 42 days. On 24d all hyperlipid diet were removed, and only normal diet given. At the end of this experiment all rabbits were killed by bleeding and the results showed that GSL could decrease the serum lipids GSL could increase the HDL - C in the rabbits injected with 60 mg/kg GSL. GSL could protect the walls of aortas from the damage of hyperlipid serum. The contents of lipid and MDA in the Walls of aortas were decreased. GSL had the effects of protection on the heart and liver by decreasing of Total Cholesterol and MDA. So we conclude that GSL has the effects of lipid regulation and lipid antipeoxidation on the chronic hy-perjipidemic rabits.

BACKGROUND:It can be confirmed by the clinical trial that chronic plantar fasciitis can be treated by extracorporeal shock wave.There are some different explains for the mechanism of action.OBJECTIVE:To introduce the mechanism of action and clinical application of extracorporeal shock wave in the treatment of chronic plantar fasciitis.RETRIEVAL STRATEGY:A computer-based search was conducted in PUBMED,HighWire and Springer databases for articles published before August 2007 with the key words of "chronic plantar fasciitis,extracorporeal shock wave application" in English by the first and second authors.Inclusive criteria:articles were selected firstly based on the mechanism of action and clinical application of extracorporeal shock wave for chronic plantar fasciitis.Exclusive criteria:articles out-of-date and repeated were removed.LITERATURE EVALUATION:Sixty-five relevant articles were collected,and twenty-five met the inclusive criteria.Of them,4 articles were review,and others were about the mechanism of action and clinical application of extracorporeal shock wave for chronic plantar fasciitis.DATA SYNTHESIS:①Someone believed that high energy shock wave selectively damaged unmyelinated sensory nerve fibers.Low energy shock wave could inhibit the conduction of sensory nerve ending by releasing calcitonin-gene-related peptide and producing neurogenic inflammation.It also could play a long-term analgesic effect by making the changes in regional flow of the brain,regulating pain memory and reducing local stimulatory response.②There are many reports on short-term and long-term outcomes,different energy levels,local anesthetic drug,the depth of plantar fasciitis and therapeutic place.CONCLUSION:It is effective to treat chronic plantar fasciitis by extracorporeal shock wave.Different approaches can bring us different therapeutic outcomes.

During lymphocyte development antigen receptor genes undergo V(D)J recombination to obtain antigen binding specificity and diversity. This process is not only controlled by genetic factors, such as tissue- and stage- specificity of RAG-1/2 protein, germline transcriptional activity and ACEs, but also regulated at epigenetic level. The chromatin accessibility of recombinase is associated with the chromatin configuration around the targeted gene segments. Thus, activation of V(D)J recombination requires the recruitment of remodeling complexes for changing the accessibility in the localized chromatin. Moreover, docking of remodeling complexes, which serve for creating active chromatin environment, relies on certain patterns of chromatin modification. Some recent findings regarding epigenetic regulation mechanisms in V(D)J recombination, such as CpG methylation, histone modification, nucleosome remodeling and nuclear topology were reviewed.

Objective To investigate the expression of uPAR and VEGF-C in hepatocellular carcinoma (HCC) and its effect on the biological behavior of HCC. Methods The expression of uPAR and VEGF-C in liver tissue specimen of 33 patients with HCC was detected by immunohistochemical staining. Results The positive expression of uPAR and VEGF-C in HCC tissues was located in cytoplasma of cancer cells. The positive rates of uPAR and VEGF-C were 69.7 %(23/33) and 78.8 %(26/33) respectively in 33 cases of HCC. The expression rates of uPAR and VEGF-C were significantly higher in HCC with metastasis than in HCC without metastasis(P<0.05). But there were not significant correlation between the expression of uPAR or VEGF-C and tumor size, tumor grade, AFP level, hepatic cirrhosis. There was a close correlation between uPAR and VEGF-C in HCC(r=0.676, P<0.01). Conclusion The expression of uPAR and VEGF-C is correlated with metastasis, uPAR and VEGF-C may be signs of aggressive phenotype for evaluating treatment and prognosis of HCC. Examination of these two proteins may be helpful to .judge biological behavior of HCC.