Objective:To explore the cognitive styles and personality characteristics of patients with peptic ulcer.Methods:The personality characteristics,defense styles, and cognitive styles of 30 patients and 30 controls were evaluated.Results:(1)The patients group got higher scores on defense mechanism of sublimation,intercourse trend,consumptive trend and self-making than the control group.(2)Logistic analysis showed that some factors of DSQ were significantly different between the patient group and control group.(3)The interaction between personality and cognitive styles was also found in the analysis.Conclusion:Personality and cognitive styles together contributed to the onset and the course of peptic ulcers.

Atherosclerosis is a chronic inflammatory disease.Atherosclerotic plaque rupture and thrombosis may result in cardio-cerebrovascular events.Inflammatory mediator CD40L widely exists in cells associated with atherosclerosis.They participate in plaque inflammatory response,release proinflammatory cytokines,degrade extracellular matrix,improve procoagulant activity,and promote the progression of atherosclerosis and plaque vulnerability.Intervening CD40/CD40L signaling system may become an effective treatment strategy to slow the progress of atherosclerosis and stabilize atherosclerotic plaques.

This paper reflected the development of modern medical technology from the view of ecology,indicating that the core idea of ecological medical model is that human and nature develop harmoniously. The theory basis,practical background and significance of ecological medical model are also described.

The use of laparoscopic liver resections for benign and malignant diseases has spread worldwide.As in open liver surgery,anatomical orientation and the ability to control intraoperative challenges as bleeding have to be combined with expertise in advanced laparoscopic techniques.We provide an overview regarding the literature on laparoscopic liver resection for benign and malignant liver tumors with the aim to discuss the current standards and define remaining challenges.Laparoscopic liver resection is feasible and safe in selected patients and experienced hands.The minimal invasive approach offers benefits in perioperative shortterm outcome without compromising oncological outcomes compared to open liver resections.Further randomized trials are needed to formally prove these statements and to define the optimal indication and techniques for the individual patient.

Macrophage migration inhibitory factor (MIF) is a cytokine of pluripotent biological behavior. With the in-depth research of MIF on molecular biology and clinic, it has been found that MIF is closely re-lated to the gastrointestinal tumor' s proliferation and invasion. In this review, we mainly discuss the correla-tion between MIF and gastrointestinal tumor.

To investigate whether the calcium channel blocker amlodipine could inhibit macrophage matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expression and secretion. Methods Peritoneal macrophages were isolated from BALB/C mice and incubated with low (5μg/L), middle (15μg/L) and high (305μg/L) concentrations of amlodipine, or in the medium alone (controls) for 24 hours, and the expression and secretion of MMP-2 and MM-9 of the cells were analyzed by RT-PCR and gelatin zymography. Results Compared with controls, amlodipine at low concentration had no significant effects on the expression and secretion of either MMP-2 and MMP-9 (P＞0.05);at middle concentrationit it could inhibited MMP-2 and MMP-9 expressions completely and significantly reduced the secretion of MMP-9 (P＜0.05); but it had no effect on the secretion of MMP-2. At high concentration it also inhibited MMP-2 and MMP-9 expression completely. Conclusion Amlodipine at 15 ìg/L inhibited the expression of MMP-2 and MMP-9 and reduced the secretion of MMP-9, suggesting that amlodipine may stabilize atherosclerotic plaque.

OBJECTIVE: To classify congenital laryngeal cysts in 62 newborn or infants, and to discuss the characteristics of different operation mode. METHOD: The clinical data of 62 infants with laryngeal cysts treated were reviewed retrospectively. After the examination of neck palpation, laryngoscope and imaging (CT or MRI), all patients were under general anesthesia of laryngeal cyst excision. we select operation mode according to the classification. Classification include: 61 cases of cysts were confined to the larynx, 1 case was beyond the larynx. Surgical managements include: an excision through external carotid approach in 1 case beyond the larynx, 61 cases had endoscopic excision under general anesthesia (12 cases with traditional bite exception, 12 cases with powered system and 37 cases with low-temperature radiofrequency ablation). RESULT: The operations was successful. No recurrence was found after 0.5 to 7 years follow-up. CONCLUSION To chose surgical management after classification and preoperative assessment, which could avoid unnecessary opening surgical approaches, reducing the recurrence, and preventing repeated endoscopic treatment or tracheotomy. The low-temperature radiofrequency ablation had the advantages of short operation time, less bleeding, little injury, low recurrence rate and light postoperative reaction. It is worthy of clinical promotion.
Child, Preschool ; Cysts ; congenital ; surgery ; Female ; Follow-Up Studies ; Humans ; Infant ; Infant, Newborn ; Laryngeal Diseases ; congenital ; surgery ; Male ; Retrospective Studies

Objective To introduce the indications and surgical technique of pedicle subtraction osteotomy (PSO) for patients with rigid kyphotic scoliosis. Methods Fifty-eight consecutive patients (21 males, 37 females) with rigid kyphotic scoliosis were operated with PSO at the apical vertebra. The average age was 14.1 years (ranged from 4 to 27 years). Among them, 31 were congenital deformity while 26 with idiopathic scoliosis and 1 with neurofibromatosis scoliosis. 9 had previous surgery history. The average preoperative Cobb's angle of scoliosis and kyphosis was measured as 83.7? and 78.2? on standing films and as 71.1? and 76.3? on the distraction films. On the bending films, scoliosis and kyphosis were corrected by 12.4% and 23.8% respectively. The associated neurologic deficits were observed in 14 patients, bony or fibrous septum in the canal was found in 6 patients on the preoperative CT or MRI. All patients underwent pedicle subtraction osteotomy at the convex side of the apical vertebra with segmental pedicle screw fixation or Luque instrumentation. The level of osteotomy varied from T8 through L1. Results Most patients were improved in terms of pain and radiographic examinations. The average follow-up period were 26.7 months in 49 cases (ranged from 5 to 69 months). The average residual angle of scoliosis and kyphosis was 30.0? and 21.3? respectively. The average correction rate of scoliosis and kyphosis was 64.2% and 63.5% respectively. The complete neurologic recovery was obtained in 11 and partial recovery in 2 at three months postoperatively. One case showed no improvement 12 months after surgery. No patients developed severe complications while 2 had pneumonia(3.4%), 2 had superior mesenteric artery syndrome (3.4%) and 5 had temporary dysfunction of one or both lower extremity (8.6%). The loss of correction was 1.8% at one-year follow-up. Conclusion Pedicle subtraction osteotomy is a reliable technique for severe and rigid kyphotic scoliosis both in adolescents and adults, and for severe congenital deformities and revision surgery. With pedicle subtraction osteotomy at the apical vertebra and segmental pedicle screw fixation, the rigid deformities can be corrected in one-stage, neurovascular complications can be greatly reduced, both the spinal balance and stability can be restored. The patients is able to ambulate with a brace as early as three weeks after surgery.

Objective To determine the effects of tacrolimus (FK506) pretreatment on the liver ischemia reperfusion (IR) injury.Methods 32 mature SD rats were randomly assigned into four groups,which were sham-operated group (S),ischemia reperfusion group (IR),low-dose FK506-treated group (L) and high-dose FK506-treated group (H).After the treatment of liver ischemia for 60 minutes and reperfusion for 6 hours,the levels of serum ALT and AST in rats were tested.The TNF-α and IL-1β levels were evaluated by enzyme linked immunosorbent assay.Liver damage was assessed by paraffin sections stained with H&E.The quantitative real-time PCR,the immunohistochemistry and Western blot were used to detect the expression of HMGB1 mRNA and protein with or without FK506 pretreatment.Results The levels of serum ALT [(424.0 ± 137.4)U/L,(291.0 ±42.0)U/L],AST [(554.2 ± 127.7)U/L,(410.2 ±7.0)U/L],TNF-α [(115.1±49.0)ng/L,120.4±28.5) ng/L] and IL-1β [(424.5 ±105.2) ng/L,(612.1 ± 49.6) rig/L] decreased markedly in the group L and group H compared with the group IR (P ＜ 0.05).The liver in the IR group showed hepatic sinusoids congestion,neutrophil infiltration and necrosis.In contrast,tissue damage of the L group and the H group was significantly decreased.The expressions of HMGB1 mRNA and protein reduced significantly when pretreatment with FK506 after reperfusion (P ＜ 0.01).However,there was no significant difference between the group L and group H (P ＞ 0.05).Conclusion FK506 pretreatment can protect the liver by reducing the expression of HMGB1,inhibiting the release of inflammatory cytokines and alleviating cell necrosis after the liver ischemia reperfusion injury in rats.

BACKGROUND:In the past, the culture and differentiation of bone marrow mesenchymal stem celsin vitrowere mostly reported in the adult or animal rather than in children.
OBJECTIVE: To explore the ability of bone marrow mesenchymal stem cels from children differentiating into neural stem cels and nerve cels.
METHODS: Bone marrow mesenchymal stem cels from children were isolated and cultured, and passage 12 cels were cultured in the pre-induction medium (DMEM culture medium containing 10% fetal bovine serum and 1 mmol/L β-mercapto ethanol) and induction medium (DMEM containing 2% dimethyl sulfoxide and 150 μmol/L butylated hydroxyanisole). Expression of nestin and β-tublin III was detected using immunocytochemistry method at 30 minutes and 7 days after induction, while RT-PCR was used to detect nestin mRNA expression at 0, 5.5, 6 days after induction.
RESULTS AND CONCLUSION: After combined induction, the cels shrank from round shape to tapered, polygonal or oval shape, and cel processes extended gradualy and became filament-like shape. Interconnected cels formed a network at 6 days after combined induction. The expression of nestin antigen was positive at 30 minutes after induction, while the expression of β-tublin was positive at 7 days. RT-PCR findings showed that positive expression of nestin mRNA was detected at 5.5 hours of induction, and then disappeared at 6 days. These findings show that the combined use of dimethyl sulfoxide and butylated hydroxyanisole can induce bone marrow mesenchymal stem cels from children to differentiate into neural stem cels and nerve cels in vitro.