1.Advances in diagnosis and treatment of incidental cholangiocarcinoma
Zeping HUANG ; Xinmin WU ; Yamin GUO
Chinese Journal of Hepatobiliary Surgery 2010;16(1):76-78
Carcinoma of gallbladder is the most frequently encountered malignancy of the biliary system. Early diagnosis is very difficult and the tumour resection rate is low,and the prognosis of carcinoma of gallbladder is very poor. With the increasingly widespread acceptance of laparoscopic cholecystectomy (LC),the number of cases of unsuspected gallbladder carcinoma (UGC) has increased. However,management of UGC is a difficult issue in the absence of established guidelines. High age and history of stones are the risk factors for gallbladder carcinoma and they are related to UGC. Surgical handling and pneumoperitoneum play an important role in the metastasis. The surgical approach used for cholecystectomy would not seem to influence the outcome in patients with UGC. The tumour stage is the most important prognostic factor. To understand UGC better, we review its clinical characteristics,investigation,prognosis and especially the recent advances in the diagnosis and treatment of this disease.
2.Effects of posterior total laminectomy decompression and reduction with pedicle screws for lower cervical fracture and dislocation
Jidong GUO ; Shuxun HOU ; Yamin SHI
Orthopedic Journal of China 2006;0(10):-
[Objective]To evaluate the indications,efficacy and safety of posterior total laminectomy decompression and reduction with pedicle screws for lower cervical fracture and dislocation.[Method]From June 2005 to February 2008,41 patients with lower cervical fracture and dislocation received posterior total laminectomy decompression and reduction with pedicle screws.The patients(M=32,F=9)were 22-47 years old,with an average of 33.5 years old.There were 38 fresh and 3 old injuries.[Result]All the patients were operated on successfully without severe complications during perioperative period.Totally 252 (89%) screws were exactly implanted in the cervical pedicle.The everage surgery time was 3.1 h (2.5-4 h).The average blood loss during the operation was 460 ml (250-950 ml). The average time of follow-up was 27.5 months(24-36 months). All patients had satisfiactory reduction and no internal fixator failure.Thirty-two patients who were followed up for more than 24 months had complete fusion. The motor and sensory score (ASIA92) were improved significantly at 2 years follow-up(P
3.Progress in researches of benzimidazole in treatment of echinococcosis
Wenjun ZHU ; Xiumin HAN ; Yamin GUO
Chinese Journal of Schistosomiasis Control 2017;29(4):530-533
Echinococcosis is a severe zoonosis parasitic disease and mainly caused by the larvae of Echinococcus granulosus and E. multilocularis. Because of the limitation of operation level,such as recurrence and death,the drug treatment is still the most important method in our country. Meanwhile,the annual number of drug treatments is far greater than surgical treatments. Drugs play a significant role in the preoperative reduction of lesions,reducing pain,extending the life of patients .This article re-views the status of animals treatment and clinical treatment of echinococcosis treated by the benzimidazole drugs and their new formulations,provides the basis for exploring the development direction of anti-echinococcosis drug treatment research.
4.Effect of long non-coding RNA ROR on proliferation and apoptosis of pancreatic cancer cells by regulating notch1 protein
Bing LI ; Zhengfang XIONG ; Yamin. GUO
The Journal of Practical Medicine 2017;33(12):1922-1927
Objective To explore the mechanism of long chain noncoding RNA RORin regulating prolifer-ation and apoptosis of pancreatic cancer cell. Methods Pancreatic cancer cell line BxPC-3 was selected. The RNA level of lncRNA ROR and notch1 was detected by RT-PCR.Notch1 protein level was detected by Western blot. The regulating relationship between lncRNA ROR and notch1 was analyzedby RNAhybird and luciferase re-porter assay. At last ,CCK-8 and TUNEL were applied to detectthe proliferation and apoptosis of cell line. Re-sults lncRNA ROR and notch1 were highly expressed in pancreatic cancer tissue ,compared with normal tis-sues. There was positive correlation between them. lncRNA ROR was over-expressed in BxPC-3,cell proliferation activity was increased and the percentagesof DNA damaged positive cells was decreased ,accompanied by in-creased levels of notch1 mRNA and protein. Luciferase assay confirmed that ROR could bind to notch1and inhibit its activity by miR-137. Compared with control group ,the proliferation of pcDNA-ROR + si-notch1 cells reduced and the proportion of TUNEL positive cells increased. The differences were statistically significant. Conclusionl ncRNA ROR regulated the proliferation and apoptosis of pancreatic cancer cells by promoting the expression of notch1.
5.Surgery combined with albendazole liposomes for advanced hepatic alveolar echinococcosis
Shunyun ZHAO ; Yamin GUO ; Bing LI
Chinese Journal of Hepatobiliary Surgery 2015;21(5):321-323
Objective To investigate the outcomes of surgery combined with albendazole liposomes in the treatment of advanced hepatic alveolar echinococcosis.Methods This is a retrospective study on 71 patients with advanced hepatic alveolar echinococcosis treated from January 2002 to November 2013.The patients were divided into 4 groups according to the surgical methods and whether surgery was combined with albendazole liposomes.Results On follow-up from 5 ~ 44 months,the mortality rates for the volume-reduction surgery group was 82.3%,the volume-reduction surgery + albendazole group 52.3%,the radical operation group 28.5%,and the radical operation + albendazole group 21.1%.The mortality rate of the radical operation + albendazole group,when compared with the radical operation group and the volume-reduction surgery + albendazole group was significantly different (P < 0.05).The mortality rate of the radical operation group,when compared with the volume-reduction surgery + albendazole group showed no significant difference (P > 0.05).The mortality rate of the volume-reduction surgery group,when compared with the volume-reduction surgery + albendazole group,the radical operation group,and the radical operation + albendazole group was significantly different (P < 0.05).Conclusions The treatment of radical operation combined with albendazole liposomes for advanced hepatic alveolar echinococcosis reduced recurrence,improved quality of life and prolonged survival.It is a feasible method to treat these patients.
6.Initial Study of Diagnostic Value of Diffusion Weighted Imaging in Pancreatic Cancer
Yamin LI ; Shunlin GUO ; Junqiang LEI
Journal of Practical Radiology 2001;0(08):-
Objective To evaluate the value of diffusion weighted imaging (DWI) and apparent diffusion coefficient(ADC) in diagnosing pancreatic carcinoma. Methods DWI was performed in 25 cases of pancreatic cancer, confirmed by pathology, and 20 normal persons. Five sequences of echo planar diffusion imaging were used, b value (?10-3s/mm2 ) was 50, 100, 300, 500, and 700 respectively. Then the ADC values in tumor area, non-tumor area and normal pancreatic area were calculated.Results ADC values of tumor tissue were significantly different from that of the tissue in the same pancreas, when b value were 100 and 300 s/mm2; there was statistically significantly difference between tumor group and normal group when b value were 100, 300, 500 and 700 s/mm2. ADC values of the tissue except for tumor tissue in the same pancreas were significant different from that of normal group when b value were 500 and 700 s/mm2. Conclusion DWI is a rapid and feasible method in detecting pancreatic cancer.
7.Analysis of deafness-related gene mutations in 88 non-syndromic hearing loss patients in Fuzhou city
Wenjin LIN ; Shunmin GUO ; Xiaomei XU ; Rongqing XU ; Yamin ZHANG
Chinese Archives of Otolaryngology-Head and Neck Surgery 2016;23(6):335-337
OBJECTIVE To investigate the deafness-related gene mutation frequency and hotspots in patients of Fuzhou city with non-syndromic hearing loss (NSHL). METHODS Peripheral blood samples were obtained from 88 cases of patients with hearing loss after clinical history inquiry and clinical examination. Their genomic DNA was extracted from peripheral blood by extraction kits to undergo polymerase chain reaction, traditional capillary electrophoresis sequencing and High-throughput sequencing so as to detect the mutations of deafness-related gene. RESULTS Among the 88 patients with NSHL, the gene mutation frequency was 34.09%.In the patients, 14 cases had mitochondrial 12 S rRNA mutations, six cases had GJB2 gene mutations and three cases had SLC26A4 mutations, two cases had MYO15A mutations, the other five cases had MYO7A, OTOF, TECTA, TMC1 and ILDR1 gene mutation respectively. CONCLUSION Among the 88 patients with NSHL, the most frequent mutation causing hereditary deadness was mutation in mitochondrial 12 S rRNA, followed by GJB2 and SLC26A4, The other genes such as MYO7A, OTOF, TECTA, TMC1 and ILDR1 gene were infrequent. The study could provide theoretical reference in genetic diagnosis, prevention and cure of hearing loss.
8.Control of Hyperglycemia in Patients with Severe Acute Pancreatitis
Yuliang AN ; Hongjian YAN ; Yaqing GUO ; Yamin ZHAO ; Jing DING
China Pharmacy 2005;0(20):-
OBJECTIVE: To discuss the the control of hyperglycemia in patients with severe acute pancreatitis(SAP).METHODS: Patients were treated with NPH(subcutaneous injection) or continuous subcutaneous insulin injection(CSII) or Insulin glargine(subcutaneous injection).Incidences of hypoglycemia and hyperglycemia and the blood sugar level within 5 days of admission were measured.RESULTS: The incidences of blood glucose level being ≤4.0 mmol?L-1 or ≥11.1 mmol?L-1 were significant different in CSII group and insulin glargine group compared with NPH group respectively(P
9.Effect of Lipopolysaccharide on Ski Expression in Rats' Astrocytes
Xin ZHAO ; Yongqiang GUO ; Ming WANG ; Tiege CHEN ; Yamin WU ; Haihong ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2017;23(5):514-519
Objective To explore how and where ski expresses under lipopolysaccharide (LPS) in rats' astrocytes. Methods Astrocytes were obtained from cerebral cortex of a newborn (within 3 days) Sprague-Dawley rat and cultured in vitro. Astrocytes were cultured with LPS in concentration of 0μg/ml, 0.001μg/ml, 0.01μg/ml, 0.1μg/ml, 1μg/ml, 10μg/ml and 100μg/ml for six hours;and cultured with LPS in concentration of 0.1μg/ml for 0 day, 2 days, 4 days, 6 days and 8 days. The level of ski was determined with Western blotting, and the lo-cation of ski was detected with indirect immunofluorescent staining. Results The expression of ski was induced by LPS, especially in the concentration of 0.1μg/ml. The expression of ski induced with 0.1μg/ml LPS peaked at 4 days of inducement and then decreased. Ski was mainly observed in nuclear in the normal astrocytes and the astrocytes induced with 0.1μg/ml LPS for 6 days. However, it was observed in cytoplasm 2 and 4 days of inducement. Conclusion LPS could induce the expression of ski in rats' astrocytes, which may participate in in-flammation.
10.Effect of Knocking Down ski on Migration of Astrocytes in Rats
Xin ZHAO ; Xingwen WANG ; Jiangli KOU ; Zhonghao LI ; Yongqiang GUO ; Yamin WU ; Haihong ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2017;23(8):905-911
Objective To investigate the effect of ski gene in migration process of astrocytes in rats. Methods Astrocytes were obtained from rats' cerebral cortex and cultured in vitro. siRNA targeting ski gene and negative control sequences were prepared. The ski-siRNA group, siRNA negative control group and untreated group were set in this experiment. The specific siRNA targeting ski gene was transfected into astrocytes with Lipofectamine?RNAiMAX Reagent. Then the ski protein levels were determined with Western blotting. After transfec-tion, the changes in migration of astrocytes were measured with wound scratch assay and Transwell migration assay. Results Western blot-ting showed that the expression of ski protein was significantly lower in the ski-siRNA group than in the siRNA negative control group and untreated group (F=132.957, P<0.001). Transwell migration assay showed that the number of astrocytes crossing through chambers was less in the ski-siRNA group than in the siRNA negative control group and untreated group (F>47.197, P<0.05). Wound scratch assay showed that the wound healing rate was lower in the ski-siRNA group than in the control group one, two, three, four and five days after transfection (F>69.187, P<0.001). Conclusion Ski knocked down by siRNA could inhibit the migration ability of astrocytes. It is a reminding that ski may take part in the migration process of astrocytes, and moreover, ski may play an important role in the formation of glial scar.