Objective To investigate the effect of montelukast sodium chewable tablets on pulmonary function in children with allergic asthma . Methods 86 cases of children with allergic asthma were randomLy divided into 2 groups, 43 cases in the control group were treated with routine clinical treatment, 43 cases in the experiment group received more with montelukast sodium chewable tablets.Serum T lymphocyte subsets, IL-6, TNF-α, IL-4 concentration levels, as well as FVC, FEV1, PEF levels and clinical efficacy were compared of two groups pre-and post-treatment.Results Compared with pre-treatment, Serum CD4 +, CD4 +/CD8 +, FVC, FEV1 and PEF levels in 2 groups increased, serum CD8 +, IL-4, IL-6, TNF-a levels decreased, compared with the control group, serum CD4 +, CD4 +/CD8 +, FVC, FEV1, PEF level and the effective rate in the experiment group post-treatment were higher, serum CD8 +, IL-4, IL-6, TNF-αlevels and the ineffective rate were lower, the differences were statistically significant (P<0.05).Conclusion Montelukast sodium chewable tablets can effectively improve the imbalance of immune function in children with allergic asthma , improve lung function, and has good clinical efficacy.

Objective To investigate the prevalences of metabolic disorders of glucose and lipids in the first degree relatives (FDP) of type 2 diabetic patients in Qingdao,China. Methods 1392 subjects were recruited,including 528 T2DM-FDR(parent and/or siblings have T2DM) and 864 NC-FDR(parent and siblings did not have DM). All subjects received both standard 75g oral glucose tolerance test and measurements of body weight,height,waist and hip circumference,percentage of body fat,blood pressure,blood lipid,and plasma uric acid. Results (1) The prevalence of diabetes was higher in DM-FDR than in NC-FDR (24.24% vs 17.01%,P

ObjectiveTo evaluate the relationship between waist to stature ratio (WSR) and hypertension,diabetes,dyslipidemia in Qingdao. MethodsData were collected from a 2001 - 2007 Qingdao area diabetes survey,population-based cross-sectional study,and 30 712 Chinese adults aged ＞ 18 years old were enrolled.Correlation analysis of BMI,WSR,hip circumference,waist circumference,waist to hip ratio (WHR) with blood glucose,blood pressure,blood lipid were conducted.ROC curve analysis in diabetes,bypertension,dyslipidemia and multivariate logistic regression analysis were also conducted.ResultsAnthropometric indicators were related with hypertension,diabetes and dyslipidemia in both men and women.Comparing with other anthropometric indicators,WSR was found to have the largest area under the ROC curve and the best cut-off point of WSR was 0.52.Multivariate logistic regression analysis showed that, after controlling age, disease history, physicalactivity, sex, thediabeteshypertension and dyslipidemia risk OR of WSR≥0.52 were largest.ConclusionsAnthropometric indicators intimately related with cardiovascular risk factors in Qingdao region,and may predict and evaluate the risk of cardiovascular disease.WSR may be the best index for predicting cardiovascular risk factors in Qingdao area.The optimal WSR cut off point for identifying cardiovascular risk factors clustering is 0.52.

This study was aimed to compare the expectorant and antitussive actions ofPlatycodon grandilforum from different production areas in order to provide references for its cultivation and production. The antitussive activities ofP. grandilforum water extract from different areas were investigated through testing the cough times induced by ammonium hydroxide in mice. And the expectorant activities were studied by testing the amount of tracheal phenolsulfonphthalein excretion in mice. The results showed that the minimum effective dose ofP. grandiflorum fromChifeng of Inner Mongolia was 0.2 g·kg-1. Under this dosage,P. grandiflorum fromChifeng of Inner Mongolia can significantly reduce the cough frequency in mice (P < 0.01), and significantly prolong the cough incubation period in mice (P < 0.01).P. grandiflorum fromSichuan province,Shangzhou ofShaanxi province andChongqing can significantly decrease the cough frequency in mice (P < 0.05). P. grandiflorum from Sichuan province,Shangzhou ofShaanxi province andChifeng of Inner Mongolia can significantly increase the phenolsulfonphthalein excretion quantity in mice (P < 0.05). It was concluded thatP. grandilforum was effective for relieving cough and removing sputum. The effect ofP. grandilforum fromChifeng of Inner Mongolia was obviously stronger than that from other areas.

OBJECTIVE:To evaluate the effects and safety of Bencycloquidium bromide nasal spray in the treatment of acute rhinitis after a cold. METHODS:A multicenter,dose parallel controlled,randomized,double-blind,placebo-controlled clinical tri-al was conducted. Two hundred and thirty-eight patients with acute rhinitis after a cold were selected and divided into group A(24 cases),B(24 cases),C(24 cases),D(24 cases),E(23 cases),F(24 cases),G(23 cases),H(24 cases),I(24 cases),J (24 cases). Group A-C were given Bencycloquidium bromide nasal spray 22.5μg,45μg,90μg,respectively,bid,spraying it once for each nostril. Group D-F were given Bencycloquidium bromide nasal spray 22.5 μg,45 μg,90 μg,respectively,tid,spraying it once for each nostril. Group G-I were given Bencycloquidium bromide nasal spray 22.5 μg,45 μg,90 μg,respectively,qid,spray-ing it once for each nostril. Group J was given placebo. All groups were treated for(4±1)d. Rhinorrhea score and continuous rhi-norrhea duration were compared among 10 groups,and the safety was evaluated. RESULTS:The rhinorrhea score and continuous rhinorrhea duration of 10 groups were improved significantly,with statistical significance (P<0.05). The improvement and trend of group I was slightly better than other 9 groups,without statistical significance(P>0.05). There was no statistical significance in the incidence of ADR among 10 groups(P>0.05). CONCLUSIONS:Bencycloquidium bromide nasal spray with 90 μg,qid times significantly improves rhinorrhea score and continuous rhinorrhea duration with good safety.

Objective Using meta-analysis to assess the association between maternal 5,10-methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism and the risk of offspring congenital heart defect (CHD) in Chinese population.Method PubMed,Embase,Web of science,Chinese Biomedical Literature Database,Chinese Science and Technology Academic Journal,Chinese Journal Full-text Database and Wanfang database were searched extensively from since their inception to January 2017 for relevant case-control studies.Odds ratio (OR) of MTHFR C677T genotype distributions in patients with CHD and healthy controls was calculated.Pooled OR calculation was analyzed by revMan 5.3software.Result A meta-analysis of nine case-control studies included 1 221 cases and 1 108 controls.There was association between maternal MTHFR gene C677T and the CHD of offspring and the pooled OR (95 ％ CI) of maternal TT/TC,TT/CC,TC/CC,TT/TC + CC and TT + TC/CC were 1.85 (95 ％ CI 1.50 ～2.28),2.33 (95％CI 1.81 ～3.00),1.24 (95％CI 1.00 ～ 1.54),2.00 (95％ CI 1.64 ～2.44) and 1.55 (95 ％ CI 1.27 ～ 1.89) (P ＜ 0.05),respectively.Conclusion There was association between maternal MTHFR C677T polymorphism and risk of offspring CHD in the Chinese population.

Objective To establish a fluorescent antibody virus neutralization (FAVN) test for hantavirus (HV).Methods With the method of dilution serum and fixed virus,the serum samples were diluted with different concentration,and with virus-liquid were mixed in 96 culture plate hole,37 ℃ for 1 hour.Then the mixture of serum and virus was added to Veto-E6 cells which had grown into a monolayer,and cultivated in the 37 ℃,5％ CO2 incubator for 7 days; the 96-well plate was fixed and stained.The results were observed under a fluorescence microscope.The neutralization titer was calculated according to the formula.Results The suitable cell concentrations is 4 × 104 ～ 4.5 × 104/hole,and the proper temperature for neutralize is 37 ℃.30 serum specimens of HFRS were tested by FAVN and PRNT,the FAVN is more sensitive and stable.Conclusion FAVN is easier,faster and more sensitive than the others.

Dengue viruses(DENV) are seriously affected by cell autophagy in intracellular replication,transmission between host,and the pathogenic mechanism.Here,the latest research progress of complex interplay between DENV and cell autophagy were reviewed in this paper.Autophagy,a cellular procedural protein degradation pathway generally possessed by all eukaryotes plays a key role in response to outside pressure and maintaining cellular homeostasis.This catabolic pathway also promotes cell survival against the invasion of pathogens such as various viruses.However,autophagy plays an opposite role in the replication of DENV.Autophagy not only provides a replication site for viruses to replicate,but also provides a source of energy for this process.Cell autophagy also participates in the pathogenesis of some DENV infection.In short,autophagy contributes to the replication of DENV.

OBJECTIVE To illuminate the adenoid bacteria distribution in children with adenoid hypertrophy. METHODS PubMed, Embash, Medline, CNKI, VIP Information and Wanfang data were searched for studies on the adenoid bacteria distribution and adenoid hypertrophy. Random effects meta-analysis was used to pool data. RESULTS Nine studies were included in this meta analysis. The pooled detection rates of haemophilus influenza, staphylococcus aureus and streptococcus pneumonia were 0.21 (95%CI, 0.09-0.32), 0.14 (95%CI, 0.09-0.20) and 0.15 (95%CI , 0.08-0.22) respectively. CONCLUSION Haemophilus influenzae, staphylococcus aureus, and streptococcus pneumoniae are three main kinds of pathogenic bacteria of adenoid hypertrophy in children.

Objective To evaluate the effect of individualized food composition table on serum phosphorus management for patients undergoing peritoneal dialysis(PD). Methods A total of 83 patients who were treated with PD for over 3 months with serum phosphorus greater than 1.78 mmol/L were included from March to May 2016 in PD Center of the Second Hospital of Hebei Medical University by convenient sampling. They received dietary guidance with individualized food composition table and stable phosphorus binding agent. After 6 months, level of serum phosphorus, biochemical indicators and other PD indicators before and after the intervention were compared. Results After 6 months of diet intervention, the patients' serum phosphorus achieved rate was 71%. Serum phosphorus, potassium, parathyroid hormone decreased, and hemoglobin, serum albumin increased (P<0.05). For the PD indicators, blood pressure control was improved (P<0.05), and the rest of the indicators had no difference compared with those before intervention (P>0.05). Conclusions Dietary intervention based on individual food composition table can effectively reduce the serum phosphorus levels in patients with PD,and improve the nutritional status and simplify education working process of the PD center diet as well.