Objective:To investigate the clinical value of magnetic resonance imaging (MRI) in differential diagnosis for benign and malignant lesions of solitary pulmonary nodules (SPN).Methods: 120 patients with SPN were enrolled in the retrospective analysis, and all of them underwent scan of MR including MRI, DCE-MR and diffusion weighted imaging (DWI) before their surgery. All of patients received detection of pathology or histopathological examination after their surgery, and the ADC values under different b values of dispersion sensitivity factor (300 s/mm2, 500 s/mm2 and 800 s/mm2) were compared.Results:Under the conditions both of 300 s/mm2 and 800 s/mm2, the differences of ADC value between benign nodule and malignant nodule were not statistically significant (t=1.195,P>0.05). And under the condition of 500 s/mm2, the ADC value of benign nodule was significantly higher than that of malignant nodule (t=4.313,P<0.05), and at this time, the sensitivity, specificity, rate of missed diagnosis and misdiagnosis rate of the differential diagnosis for benign and malignant lesions of SPN by using MR were 85.71％, 74％, 14.9％ and 26％, respectively.Conclusion: MR examination has higher sensitivity in differential diagnosis for benign and malignant lesions of SPN, and it has a certain clinical value when it is used as early detecting method.

Objective: To study whether the infection of Schistosomiasis japanicum (S. japanicum) is related to enhanced proliferation and migration of cancer cells, and the molecular mechanism pertains to cancer cell metastasis in human host. Methods: The gene of S. japanicum glutathione transferase (sjGST) cloned from S. japanicum was expressed, purified and applied in a series of assays to explore the effect of sjGST on proliferation and migration of MDA-MB-435S, and the expression of MMP2 and MMP9. Immunofluorescence assay for the binding of sjGST to MDA-MB-435S was also carried out. Results: Results showed that sjGST enhanced proliferation and migration in human breast cancer cell MDA-MB-435S signifycantly at 50-200 nM, but did not enhance them in human lung cancer cell A549. Immunofluorescence assay for the binding of sjGST to MDA-MB-435S and A549 showed that GST was readily bound to the breast cancer cells, but showed almost no binding to human lung cancer cells. The assays for gelatinase activity showed that both MMP2 and MMP9 activities were increased significantly in the presence of sjGST (50-200 nM) in MDA-MB-435S, but they were not significant in A549. Conclusions: Our current results show strongly that S. japanicum GST binds to MDA-MB-435S probably via its receptor, and enhances proliferation and migration of the cancer cells by up-regulatory expression of MMP2 and MMP9.

AIM: To evaluate the main pharmacological effect of Wunzhonganwei Soft Capsule. METHODS: Analgesic effect was investigated by hot plate test and writhing test, anti-inflammation effect by auricular swelling test in mice and toe swelling test in rat, anti-diarrhea effect by diarrhea test induced by rhubarb, effect on gastric emptying by phenolsulfonphthalein empty test in mice and effect on small intestine propulsive test by charcol powder propulsive rate test. RESULTS: Wunzhonganwei Soft Capsule enhanced thermal stimulation threshold in mice, decreased the occurrence of writhing caused by glacial acetic acid in mice, inhabited xylene-induced auricular swelling in mice and carrageenan-induced toe swelling in rat, decreased the number of loose stools induced by rhubarb in mice., inhabited the function of gastric emptying induced by metoclopramide or in normal mice, antagonized the inhibitory effect of gastric emptying induced by atropine and inhabited small intestine propulsive movement induced by neostigmineor or in normal mice. CONCLUSION: Wunzhonganwei Soft Capsule has the effect of anti-inflammation, analgesia, antidiarrhea and adjusting the function of gastrointestinal movement.

AIM: To investigate the action of Xingshen Capsule(Tea, Radix Ginseng, etc.) on anti-centre and body fatigue in mice. METHODS: Mice were divided into five groups and were given different dosage once a day for six days, then, their weight, motor activity, roating bar tolerance and the hypnogenetic effects of sodium pentobarbital were observed on the 1 st and 6 th day. RESULTS: Comparing with the negative control, Xingshen Capsule could notably increase the times of motor activity, prolong the persisting time on the roating bar, shorten the sleeping time caused by sodium pentobarbital while prolong its latence. CONCLUSION: Xingshen Capsule may have outstanding effect on refreshing one's nerve and body.

AIM: To investigate the effect of Salidroside on the proliferation, DNA synthesis, intracellular Ca 2+ content of rabbit PASMC (pulmonary artery smooth muscle cells) under hypoxia. METHODS: Techniques of cell culture, MTT test, -TdR incorporation, fluo-3 and confocal laser scanning microscopy were used. RESULTS: The A value of MTT and -TdR incorporation of PASMC increased significantly by 62% (P

Objectives To compare the difference between multipoint inoculation and routine method for isolation of pathogenic fungi from nail samples of onychomycosis,and to analyze the epidemiology of pathogenic fungi in those patients.Methods The nail clipping samples from each patient were inoculated onto the plates with Sabouraud's agar,Sabouraud's agar without cycloheximide and medium containing rapeseed oil,respectively,by an approach of at least seven inoculating points in each plate (multipoint inoculation),and onto medium slope in tubes with the same media as above mentioned (routine method).In the multipoint inoculation method,plates with more than 3 colonies were taken for further identification of pathogenic fungi based on morphological and biochemical properties.Results Based on the data from 150 samples of onychomycosis,significant differences were found between multipoint inoculation method and routine method (P

AIM: To study the effects of Rhodiola(Rho), nitric oxid e (NO), hem oglobin (HB) on the multiple organ dysfunction syndrome(MODS) in early stage aft er burn in rabbits. METHODS:The rabbits were divided into the sh am burn group (SB), burn group (B), orally taken Rho group (R), burn and Rho therapy group (BR ). The changes of hemodynamics were monitored. The index of pulmonary permeabili ty was calculated. These data reflected separately the functions of heart, live r, lung, kidney and blood coagulation system were also determined. NO contents i n ser um and bronchoalveolar lavage fluid (BALF) were measured by Griess method. The l e vels of serum HB were measured. RESULTS:① The dysfunctions of hear t, liver and kidney achieved the criterion of MODS in group B at 48 h postburns (B 48 h ). The N O content of group B significantly increased in serum and BALF at B 48 h . ② The cardiac index (CI) and creatine phosphokinase (CK), urea nitrogen (BUN) markedly raised or decreased in group BR at 48 h postburns (BR 48 h ) than B 48 h . The NO cont ents in serum and BALF markedly raised. ③ HB contents in serum markedly raised in group B and BR at 0 h postburns (B 0 h , BR 0 h ) than group SB, B 48 h , R, BR 48 h , but NO was reverse. CONCLUTIONS: ① HB contents in serum markedly raise d at 0 h post burns, but NO was reverse. ② Rho promoted the increases of NO synthesis and the blood perfusion of organs, which might be one of mechanisms to prevent the devel opment of MODS.

OBJECTIVE To study the relationship between Gardnerella vaginalis and male infertility. METHODS To test G.vaginalis in semen by nested PCR and to carry out correlation analysis with sperm parameter,volumetric count of leukocytes and AsAb.Totally 383 male cases with infertility,set up into positive group,other 297 cases set up into negative group. RESULTS G.vaginalis in semen was positive in 86 cases in positive group,their semen concentration,motility,a+b grade motile,and the swelling rate of hypoosmotic swelling test were observed to be evidently lower compared to negative group(P0.05). CONCLUSIONS G.vaginalis has an effect on sperm quality.

Objective To summarize and analyse the clinical characteristics,diagnosis and treatment options of neuroendocrine carcinoma of gallbladder (GB-NEC).Methods The data of 10 patients with GB-NEC treated in our hospital between December 2007 and December 2012 were retrospectively analyzed and compared with the data of 377 patients with gallbladder adenocarcinoma.Results The 10 patients with GB-NEC accounted for 2.2％ (10/464) of all gallbladder cancers that were treated during the study period.The mean age was 59.0 ± 10.0 years,the ratio of male to female was 1 ∶ 4.All the 10 patients had low-differentiated small cell NEC,four patients were accompanied with an adenocarcinoma.Immunohistochemical examinations showed that the positive rates of CgA,NSE,Syn,EMA were 100％ (10/10),100％ (10/10),88.9％ (8/9) and 87.5％ (7/8),respectively.One,two and seven patients had a TNM grade Ⅱ,ⅣA,and ⅣB,respectively.Seven patients (70.0％) had N2 lymphatic metastases,which was significantly higher than the percentage in patients with gallbladder adenocarcinoma (128/377,34.0％ ; P ＜ 0.05).Two patients were treated with radical resection,while the other 8 patients were treated with palliative surgery.The 1-,2-,and 3-year survival rates of the patients with GB-NEC were 20.0％,10.0％,and 0,respectively.The median survival time was 92 days.In contrast,the 1-,2-,3-,and 5-year survival rates of the 377 patients with gallbladder adenocarcinoma were 32.7％,25.4％,21.3％,and 11.7％ respectively,and the median survival time was 180 days.Condusions GB-NEC was found mainly in aged females.The clinical presentations were mainly non-specific,and immunohistochemical examinations were needed for a definite diagnosis.GB-NEC is highly malignant,and local invasion and lymphatic metastasis could occur early.The short-term recurrence rate was very high.The prognosis of GB-NEC was poorer than gallbladder adenocarcinoma,while surgical resection combined with radiotherapy,chemotherapy and TACE increased the survival of these patients.

Objective To explore the effect of low tidal volume ventilation on the treatment of children with acute respiratory distress syndrome (ARDS).Methods Forty-four hospitalized children with ARDS from Jan 2008 to Dec 2011 at Kunming Children's Hospital were enrolled in this study.The observation group included 24 patients who received the treatment of low tidal volume ventilation (6 ～ 8 ml/kg),while the other 20 patients were in the control group who were given traditional volume ventilation (10 ～ 12 ml/kg).Oxygenation situations and blood gas analysis at 24,48,72 h after ventilation、mechanical ventilation complications,and the mortality in the first 28 days were observed.Results The ratios of PaO2/FiO2 were 104 ±23.6 in observation group and 112 ±34.7 in control group (P ＞0.05).However,after 24,48 and 72 h of ventilation,the ratios of PaO2/FiO2 showed statistical significance (t ≥2.01,P ＜0.05 ; t ≥2.74,P ＜ 0.01).Barotrauma in observation group was significantly lower than that in control group (x2 =6.97,P ＜ 0.01).The mortality (16.7％) of the observation group was significantly lower than that (50％) of the control group (x2 =5.58,P ＜ 0.05).Conclusions Low tidal volume ventilation can reduce complications and improve oxygenation of ARDS patients,and reduce the mortality in ARDS children.The improved rescue technology should be applied for the treatment of children with ARDS.