Objective To observe the effect of Spleen-kidney Bi-remedial Pellets on the ulcerative colitis model, and provide experimental proof for the efficiency of clinical practices. Methods To induce mouse to diarrhea by Fanxieye, then observe the effect of Spleen-kidney Bi-remedial Pellets. Ulcerative colitis on mouse was caused by partial bowel-enema of TNBS and 50% ethanol, then observe the effect of Spleen-kidney Bi-remedial Pellets on IgG and IgM. Results Spleen-kidney Bi-remedial Pellets was capable to obviously reduce the diarrhea on mouse by Fanxieye, with a remarkable difference to the comparative group (P

Objective: To study the effects of ureolysis on the proliferation and acid-resistance of Actinomyces naeslundii.Methods:The growth of Actinomyces naeslundii cultured in different nitrogen sources were measured, and the acid-resistant ability of Actinomyces naeslundii cultured in acid condition with different urea concentration was compared.Results:In contrast with other nitrogen sources,Actinomyces naeslundii could be cultured by urea and higher final A value of the bacteria was obtained;at the range of pH=4.0-7.0, ureolysis could increase the survival of Actinomyces naeslundii from acid killing to some extend, but at pH=3.0, ureolysis could not protect Actinomyces naeslundii anymore.Conclusion:Ureolysis may stimulate the growth of A. naeslundii and increase the acid-resistant ability of Actinomyces naeslundii.

Based on the analysis of the problems of traditional medical literature retrieval teaching,taking North Sichuan Medical College as an example,the paper discusses the reform practice of medical literature retrieval course teaching from the aspects of enriching teaching contents,constructing literature retrieval excellent resource shared course,online practice and examination platform and controlling teaching quality strictly,etc.

〔Abstract〕 Taking North Sichuan Medical College as an example, the paper analyzes the current situation and problems in computer network basis and application teaching.Combining with teaching practice, according to the characteristics of medical students, it elabo-rates teaching reform which involves teaching idea, plans, contents and methods, etc.

Team-oriented scientific project competition-based ideas and measures in teaching of Medical Information Analysis course were studied by introducing non-medical cases into discussion and analysis , simulating brain storm conference , and supplementing team competition .

Objective:To explore the expression of ROR?t in pulmonary tissue of asthmatic mice and to investigate the association between the expression of ROR?t and the airway inflammation.Methods:Thirty female BLAB/c mice were randomly divided into the control group,asthmatic group and dexamethasone (Dex)-treated group.The asthma model was induced by classical method with ovalbumin(OVA).The concentration of IL-17 in bronchoalveolar lavage fluid (BALF) and serum was measured by enzyme-linked immunosorbent assay(ELISA).Airway inflammation was evaluated by HE staining.The expressions of IL-17,ROR?t mRNA and protein was measured by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot respectively.Results:The level of IL-17,ROR?t mRNA and protein of asthmatic group was significantly higher than those of control group and Dexamethasone treated group (P

AIM: To investigate the influence and mechanism of recombinant macrophage migration inhibitory factor(rMIF) on fibroblasts.METHODS: MRC-5 fibroblasts were divided into two groups: the treated group was treated with rMIF(25-100 ?g/L,12 h,24 h or 48 h) and the control was non-rMIF treatment.The activity of proliferation in both groups was investigated and compared by CCK-8 means.Synthesis of collagen in the culture supernatants was detected by the hydroxyproline.The expression of collagen type I mRNA was examined using RT-PCR analysis.The level of collagen type I protein induced by rMIF was quantified by Western blotting.RESULTS: The production of proliferation ratio of fibroblasts treated with 50 ?g/L and 100 ?g/L rMIF at 24 h or 48 h were increased obviously(P

OBJECTIVE: To evaluate the efficacy of sublingual immunotherapy (SLIT) with Dermatophagoides farinae drops for allergic rhinitis (AR) of different symptom severity. METHOD: This retrospective analysis to receive SLIT treatment of 143 cases of patients with allergic rhinitis, according to the severity of disease symptoms divid- ed into two groups, moderate group (62 patients) and severe group (81 patients). Before SLIT and after SLIT for half year, 1 year and 1. 5-2.0 years, the TNSS, TMS and sign scores of patients with allergic rhinitis were evaluated. RESULT: The TNSS, TMS and sign scores had continuously improved significantly after SLIT for half year, 1 year and 1.5-2.0 years in two groups as compared with baseline (P < 0.05). Before SLIT, TNSS and sign scores of severe group had a significantly higher level than moderate group (Z = 10.40, 2.40, P < 0.05), while TMS of two groups had no significant differences (Z = 0.00, P > 0.05). Half year after SLIT treatment, in two groups for sign scores, there were significant differences (Z = 3.32, P < 0.05), and there were no significant differences for TNSS (Z = 1.58, P > 0.05) and TMS (Z = 0.37, P > 0.05). 1 and 1.5-2.0 years after SLIT, there were no significant differences in two groups for TNSS, TMS and sign scores (P > 0.05). CONCLUSION SLIT with Dermatophagoides farinae drops for 1.5-2.0 years is effective in the patients with allergic rhinitis of different symptom severity. And equivalent efficacy could be achieved for different symptom severity.
Administration, Sublingual ; Animals ; Antigens, Dermatophagoides ; administration & dosage ; Dermatophagoides farinae ; Humans ; Retrospective Studies ; Rhinitis, Allergic, Perennial ; drug therapy ; Sublingual Immunotherapy ; Treatment Outcome

Objectives The cellular repressor of E1A-activated genes (CREG), a novel gene, was recently found to play a role in inhibiting cell growth and promoting cell differentiation. The purpose of this study was to obtain antibody against CREG protein and to study the expression of CREG protein in human internal thoracic artery cells (HITASY) which express different patterns of differentiation markers after serum withdrawal. Methods The open reading frame of CREG gene sequence was amplified by PCR and cloned into the pGEX-4T-1 vector. Glutathione-S-transferase (GST)-CREG fusion protein was expressed in E. Coli BL21 and purified from inclusion bodies by Sephacryl S-200 chromatography. Rabbits were immunized with the purified GST-CREG protein. Western blot examined with immunohistochemistry staining and the protein expression level was analyzed by Western blot in HITASY cells after serum removal. Results It was confirmed by using endonuclease digesting and DNA sequencing that the PCR product of CREG was correctly inserted into the vector. The GST-CREG protein was purified with gel filtration chromatography. Polyclonal antibody against GST-CREG was obtained from rabbits. CREG protein immunohistochemistry staining displayed a perinuclear distribution in the cytoplasm of HITASY cells. Results from Western blot suggested that comparing with the untreated cells upregulation of CREG polyclonal antibody against CREG was comfirmed. Using this antibody, the changes of CREG protein expression was observed in the process of phenotypic modulation of HITASY cells. These results provide basic understanding on the relationship of CREG gene with the cell phenotypic conversion.

Objective To investigate the effect of intervention management of hypertension and related risk factors.Methods One hundred and twenty hypertension patients are intervened in terms of blood uric acid,unbalanced diet,overweight,obesity,smoking,excessive drinking,stress and lack of physical activity.And after the medication,comparative analysis is carried out after 1 years follow-up supervision.Results There were significant differences in terms of systolic blood pressure,diastolic blood pressure,waist circumference,body mass index(BMI),fasting blood glucose,total cholesterol,low density lipoprotein (LDL),triglyceride (TG),high density lipoprotein (HDL),blood uric acid and halophilic,stroke like adaptation and cognitive ability in hypertension before and after intervention (t =10.44,8.93,3.98,2.76,7.82,5.39,3.11,3.88,2.24,2.73,5.31,5.11,6.44,3.60,6.58 respectively ;P ＜ 0.01).There weren't significant differences regarding of smoking habit,unregularly life style and stress (P ＞ 0.05).Conclusion The intervention on blood pressure and related risk factors based on changing lifestyles is proved to be with high efficiency in University Communities.