To explore whether the imprinting status of IGF-2 in the malignant epithelial ovarian tumors is different from that in benign tumors, the target sequences (DNA and RNA) which contain a polymorphism site for ApaI restriction endonuclease digestion were amplified with PCR and RT-PCR methods. Then the PCR/RT-PCR products were digested by ApaI. The IGF-2 transcriptional pattern came out from the results of endonucleases digestion. Among the 36 cases of benign epithelial ovarian tumors, 20 were heterozygous for ApaI locus and all showed genomic imprinting. While in the malignant group, 22 were heterozygous for ApaI locus but six were found to lose imprinting. Significant differences existed between the two groups (P < 0.05). Loss of imprinting of IGF-2 may serve as a marker for differentiating the malignant ovarian cancers from the benign ones. In a new field of molecular genetics, our research provides an experimental basis for genetic diagnosis and treatment of the ovarian cancers.
Cystadenocarcinoma, Serous/*genetics ; Cystadenoma/genetics ; *Genomic Imprinting ; Insulin-Like Growth Factor II/*genetics ; Ovarian Neoplasms/*genetics

Surface topography of the Anisakis type I 3rd stage larva (L3), the main pathogen of anisakiasis, was observed by scanning electron microscope under magnifications of 400 to 14000X. The head bears a trapezoid undivided lip mass, with 2 mammillary elevations at the middle of the two lateral sides and a cuticular boring tooth on its ventral side. The mouth leading into the esophagus is situated in the centre of the centrally located cuticular elevation of the lip mass. The excretory pore opens ventrally just behind the boring tooth. The surface of the lip mass shows fine striations, but looks smooth elsewhere. Neither flat papillae nor minute "teeth" or "hairs" described hither to have been observed. The cuticle of the body surface shows shallow and irregular annular grooves and folds and numerous fine longitudinal micro-furrows and cristae. These surface markings appear uniformly from head to tail and to the utmost end of the pagoda like mucrones at the tip of the tail. The ventrally located crescent anus is situated about 90?m from the base of the mucron.

Objective To study the properties of K~ +-ion channel of rat parietal cells, and to approach its effects on physiological and pathological process. Method Parietal cells were isolated. Electrophysiological records of K~ +-ion channels were made through whole cell's patch clamp technique, and the properties of the channels were analyzed. Results 10mM tetraethylammonium chloride may partially block the outwardly depolarized K~ +-ion channel current. While 10mM tetraethylammonium acetate, 10mM barium chloride or 10mM barium acetate may increases the outwardly depolarized K~ +-ion channel current. Conclusion The whole cell K~ +-ion channel current of rat parietal cells showed voltage sensitive. The current-voltage relations of whole cell potassium channels of rat parietal cells are both outwardly and inwardly rectified.

0.05)and the differences of AUC 0～t ,T max and C max were not significant as verified by two-one sided t test.CONCLUSION:The relative bioequivalence of polyferose capsules is101.02%which indicates that polyferose capsule is a bioequivalent product of Niferex capsule.

Objective To investigate the clinical features and drug resistance of neonatal purulent meningitis caused by Escherichia coli. Methods The clinical data of 46 neonates diagnosed with purulent meningitis caused by Escherichia coli were retrospectively analyzed from June 2004 to June 2014. The neonates were divided into community acquired infection group and nosocomial infection group, or early group (from June 2004 to May 2009) and late group (from 2009 June to June 2014). Result Fever, hypothermia, lethargy, poor feeding, tachypnea, and tachycardia were common clinical manifestations in all neonates. The detection rate of ESBLs was signiifcantly higher in nosocomial infection group than that in community acquired infection group (P?

Objective To understand the trend of occurrence,diagnosis,and prognosis of breast cancer in the cases treated in Peking Union Medical College Hospital(PUMCH) between 1975 and 2006.Methods A total of 1092 cases of breast cancer treated in the PUMCH from 1975 to 2006 were classified into six groups by time span and reviewed for a comparative study on the parameters of gender,age,menstruation status,tumor size,mumber of positive axillary nodes,pathologic stage,recurrence and metastasis and disease-free survival rate.Results The average age of breast cancer patient has risen from 50.90 to 53.92,while 40-49 year-old group was the largest age group of breast cancer patients,and the percentage of menopause female was the highest.Tumor size has gradually been smaller especially since 2000.The number of positive axillary nodes showed no significant change during the time periods.In all 6 groups,stage-II cases were more frequent,although the percentage of stage-0 and stage-I cases was on the rise,while the percentage of stage-III declined,and that of stage-IV remained stable.In all 6 groups,the 5-year and 10-year disease free survival rate had steadily increased.Conclusions The early diagnostic rate of breast cancer has been improving and subsequently the prognosis has improved as well.

Objective To evaluate effects of different laparoscopic procedures for hydrosalpinx on ovarian responses to superovulation induction during in vitro fertilization-embryo transfer(IVF-ET).Methods A total of 65 patients with infertility derived from hydrosalpinx underwent 67 cycles of IVF-ET from January 2002 to January 2005.Before the IVF-ET,the patients were given bilateral laparosocpic salpingectomy(Group A,n=47,48 cycles) or laparosocpic proximal tubal ligation and distal tubal salpingostomy(Group B,n=18,19 cycles).Another 58 patients with proximal tubal occlusion without hydrosalpinx were simultaneously included for 58 cycles of IVF-ET(Group C).Results The fertilization rates,cleavage rates,implantation rates,and clinical pregnancy rates were 85%?21%,95%?17%,49%(55/113),and 79%(38/48) in the Group A,83%?17%,98%?7%,47%(21/45),and 68%(13/19) in the Group B,and 81%?22%,92%?16%,41%(57/140),and 66%(38/58) in the Group C,respectively,without statistically significant difference among the 3 groups(F=0.48,P=0.620;F=1.22,(P=0.297);?~2=1.691,P=0.429;?~2=2.471,P=0.291).The doses of gonadotrophin,the duration of stimulation,and the number of retrieved oocytes were 29.4?10.3 vials,9.4?1.7 d,and 11.4?5.6 in the Group A,24.7?7.7 vials,9.0?1.8 d,and 12.8?6.1 in the Group B,and 28.4?8.8 vials,9.1?1.8 d,and 11.4?5.3 in the Group C,respectively,without statistically significant difference among the 3 groups(F=1.77,P=0.174;F=0.52,P=0.593;F=0.51,P=0.599).Conclusions Laparosocpic salpingectomy or laparosocpic proximal tubal ligation with distal tubal salpingostomy for hydrosalpinx does not impair ovarian responses to the superovulation induction.Both of the procedures may be considered for the treatment of hydrosalpinx prior to IVF-ET.

BACKGROUND: How to make growth factor plays a role persistently and efficiently is a key in constructing bone tissue engineered bone. Currently, varied microspheres or scaffolds were used as release carriers, however, the delayed release effects needs elevating.OBJECTIVE: To prepare chitosan microosPheres/nano-hydroxyapatite/poly (lactic-co-glycolic acid) (CMs/nHA/PLGA) scaffolds, and to measure its characteristics of delayed release of bovine serum albumin (BSA).METHODS: CMs were prepared by an emulsifying cross linking method with BSA as a model protein. Using ice particulates as porogen, composite CMs/nHA/PLGA scaffolds were prepared by freeze-drying. The characteristic and morphology of the composite were observed by scanning electron microscope, later particle size analyzer, mercury porosimeter and universal testing machine, and the release behavior of BSA was investigated in vitro.RESULTS AND CONCLUSION: The CMs were spherical shape with a regular surface, with diameters of 20-40 μm. The encapsulation efficiency of the CMs was 86.5%, and the loading capacity was 0.8%. With the increase of initial BSA dosage, the loading capacity increased to 2.6%, while the encapsulation efficiency decreased to 74.1%. The CMs can be uniformly distributed in PLGA scaffolds to form CMs/nHA/PLGA scaffolds, which had 100-400 μm pore diameter and over 80% porosity, with 1.1-2.3 pMPa compressive strength, and 26.5% cumulative degradation at 10 weeks. The cumulative release of BSA from nHA/PLGA scaffolds was above 85% at 36 hours, which from CMs was 33.6% at 10 days, and that from CMs/nHA/PLGA scaffolds was 81.5% at 40 days. The results demonstrated that CMs/nHA/PLGA scaffolds have an excellent releasing efficiency for protein drugs with suitable compressive strength and degradation, which would be used as delivery system and tissue engineering scaffolds.

BACKGROUND: Linxian County of China is one of the areas with the highest incidence of esophageal cancer and gastric cardia cancer in the world, and nutrition-deficiency is widely existing in local people. In recent years, many researches around the world revealed that the cause of Parkinson disease (PD) is related to factors of gene, age, environment, diet, nutrition and smoking. More and more studies confirmed that primary hypertension may be in relation to vascular Parkinsonism (VP) and long-term hypertension was apt to VP.OBJECTIVE: To investigate the relationship between hypertension and clinical probable Parkinson disease (PPD) in nutrition-deficient population of Linxian County and provide a theoretical basis for early prevention and treatment of PD.DESIGN: Cross-sectional study.PARTICIPANTS: A total of 4 335 subjects aged over 55 years were selected. These subjects have taken part in the nutritional intervention study of Linxian County and first entered in the cohort study in 1985. They were enrolled in the nutritional intervention study in Linxian County in 1985.METHODS: A prospective cohort study was conducted. ①Case screening: PD questionnaire (used in American Gebai County) combined with general neurological examination were adopted. ②The diagnosis of PD: Clinical diagnostic criteria of UK Parkinson Disease Society Brain Bank were taken as the criteria for screening PD. Further evaluations were undertaken for clinical PPD and clinical possible PD on subjects who had PD symptoms.The diagnostic criteria of clinical PPD: Subjects were diagnosed as having clinical PPD if they presented any two of the following two cardinal features (resting tremor, hypermyotonia, bradykinesia and impairment of postural reflexes) or presented any one of the following features (resting tremor, hypermyotonia and bradykinesia). Diagnostic criteria of clinical possible PD: Subjects were diagnosed as having clinical possible PD when presented any one of the following four cardinal features (resting tremor, hypermyotonia, bradykinesia, and impairment of postural reflexes).③Definition of hypertension: Hypertension was defined as the systolic blood pressure (SBP) ≥ 140 mm Hg or the diastolic blood pressure (DBP) ≥ 90 mm Hg. Data were processed with linear trend test and nonconditional logistic regression.MAIN OUTCOME MEASURES: Status of final diagnoses on patients and relationship between hypertension and clinical PPD.RESULTS: A total of 4 335 subjects including 2 008 males (46.32%)and 2 327 females (53.68%) participated in the screening of PD.①Results of final diagnosis on patients: Among all the 4 459 survival participants,46 subjects were diagnosed as having PD, 118 as having clinical PPD and 78 as having clinical possible PD. After excluding patients with PD or clinical possible PD, only 118 patients were diagnosed as having clinical PPD. These patients and 4 217 normal controls were analyzed and a total of 2 035 patients had hypertension. ②There were statistical correlation between hypertension and clinical PPD, RR was 1.648 (1.147-2.638), which was 1.668 (1.145-2.432) after being adjusted by possible confounding factors including age, gender, smoking, drinking and so on, the association mentioned above still existed (χ2=7.463,P=0.006). Analysis of gender showed statistically significant differences between female patients with hypertension and clinical PPD(χ2=9.669 P=0.002), and RR before adjust ment was 2.347 (1.347-4.091), which was 2.346 (1.327-4.150) after being adjusted and correlation still existed. While there were no statistical corre lations between male patients with hypertension and clinical PPD (χ2 =0.697 ,P=0.404)but there was also an ascending trend in RR value. ③ With the blood pressure increasing, the RR value correspondingly in creased with the linear trend test (χ2=11.325 ,P=0.003). And there was sta tistical significance in raw and adjusted RR value of hypertension with the BP ≥ 140/90 mm Hg. Respective statistics of SBP and DBP showed a dose-response relationship between SBP and clinical PPD; When the SBP ≥ 140 mm Hg, there were statistical significances in values of raw RR or adjusted RR of hypertension (χ2=8.007 ,P=0.018). While there were no sta tistical significances in RR values before and after adjustment of DBP (χ2 =2.569,P=0.227). CONCLUSION: Hypertension is one of the risk factors of clinical PPD in female residents older than 55 and the incidence of getting clinical PPD is increased with the heightening of BP.

Objective To purify BMSCs by a simple Colony-Forming system and identify BMSCs in vitro.Methods BMSCs were planted at low-density ( 10/cm2 ) in the early stage of isolation,additional cells were scraped until a colony formed,secondly seeded those Colony-Forming cells at low-density.This process was repeat again when passaged cells formed another colony.This so called Colony-Forming selection was repeated several times until highly purified cells were obtained.Biological characters of BMSCs were observed.The surface antigens of BMSCs were identified by flow cytometry.The multipotentiality of BMSCs was assayed for differentiation into either osteoblasts or adipocytes.Results Homogeneous cells were obtained after BMSCs were passaged twice to thrice by Colony-Forming system.These BMSCs highly expressed positive surface antigens of BMSCs ( CD29 in 98.8％,CD90 in 98.4％ ) meanwhile seldom expressed negative surface antigens of BMSCs (CD31 in 2.6％,CD45 in 3％ ).BMSCs efficiently differentiated into osteoblasts and adipocytes.Conclusions Colony-Forming system is a simple and effective way to get highly purified BMSCs in a short time.