目的探讨推拿加腰背肌训练治疗非特异性下腰痛的作用机理。方法200例非特异性下腰痛(NLBP)患者分为4组:空白组、训练组、推拿组、推拿加训练组,各50例。在治疗开始和治疗4周后,对4组患者进行肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量的测定及目测类比定级法(VAS)评定。结果4组患者的CK、LDH、SOD和MDA含量以及VAS指数均有不同程度的改善。组间比较,推拿加训练组的CK、LDH、SOD和MDA含量以及VAS指数均明显优于其他3组(均P<0.01)。结论中医推拿技术结合主动功能训练治疗NLBP具有明显的康复疗效。

Objective To observe effects of acupoint sticking of Migudan on BGP and Hyp in the patients of primary osteoporosis. Methods By computer random number generating method, the patients were randomly divided into a treatment group treated with acupoint sticking of Migudan, and a control group treated with Gaitianli, 48 cases in each group. Changes of their BGP and Hyp after treatment were investigated. Results Acupoint sticking of Migudan could increase significantly BGP of serum in the patients of primary osteoporosis as compared with the control group (P

Objective To observe the effect of modified electroconvulsive therapy combined with venlafaxine on cognitive function and its efficacy in patients with depression.Methods 60 patients with depressive disorder were selected,and they were randomly divided into study group and control group according to the random number table method,30 cases in each group.The patients in the study group accepted modified electroconvulsive therapy for 10 times combined with venlafaxine sustained-release tablets treatment,while the patients in the control group were only treated with venlafaxine.Both two groups were treated for 6 weeks.The cognitive function was assessed by the Repeat able Battery for the Assessment of Neuropsycholgical Status (RBANS) and the therapeutic effect was evaluated by Hamilton Depression(HAMD) Scale before and after treatment.Results The scores of immediate memory,delayed memory,speech function,visual breadth and attention of both two groups after treatment were significantly higher than those before treatment(all P ＜ 0.05),and the attention score of the study group was (109.96 ± 19.01) points,which was higher than (100.32 ± 17.13)points of the control group (t =2.062,P ＜ 0.05).The HAMD scores after treatment in both two groups were significantly lower compared with those before treatment (all P ＜ 0.05),and the HAMD score of the study group was (7.23 ± 4.19)points,which was lower than (10.27 ± 5.99)points of the control group (t =2.273,P ＜ 0.05).The total effective rate of the study group(96.7％) was significantly higher than that of the control group (86.7 ％) (x2 =6.546,P ＜ 0.05),and the cure rate of the study group (60.0％) was also higher than that of the control group (36.7％) (x2 =10.901,P ＜ 0.05).Conclusion Modified electroconvulsive therapy combined with venlafaxine treatment can effectively improve the cognitive function of depressive patients,and its effect is significantly better than monotherapy.

Office blood pressure has been regarded as a gold standard of blood pressure measurements.It is an important indicator of ensurement of correct diagnosis,management of condition and evaluation of prognosis.But limitations of office blood pressure have led to the increasing use of out-of-office blood pressure.Many evidence show that out-of-office blood pressure could more accurately reflex variability of blood pressure and rhythm of blood pressure.Out-of-office blood pressure could diagnose white-coat hypertensives or masked hypertensives without delay,properly determine the need for antihypertensive treatment and decrease risk of developing blood pressure-related complications.

Objective To purify BMSCs by a simple Colony-Forming system and identify BMSCs in vitro.Methods BMSCs were planted at low-density ( 10/cm2 ) in the early stage of isolation,additional cells were scraped until a colony formed,secondly seeded those Colony-Forming cells at low-density.This process was repeat again when passaged cells formed another colony.This so called Colony-Forming selection was repeated several times until highly purified cells were obtained.Biological characters of BMSCs were observed.The surface antigens of BMSCs were identified by flow cytometry.The multipotentiality of BMSCs was assayed for differentiation into either osteoblasts or adipocytes.Results Homogeneous cells were obtained after BMSCs were passaged twice to thrice by Colony-Forming system.These BMSCs highly expressed positive surface antigens of BMSCs ( CD29 in 98.8％,CD90 in 98.4％ ) meanwhile seldom expressed negative surface antigens of BMSCs (CD31 in 2.6％,CD45 in 3％ ).BMSCs efficiently differentiated into osteoblasts and adipocytes.Conclusions Colony-Forming system is a simple and effective way to get highly purified BMSCs in a short time.

Objective:Eighteen inorganic elements were measured from the rhizoma and fibrous root of Polygonatum Cyrtonema Hua. The contents of K, Fe,Mg,Ba,Cu,Mn and Bi of them in rhizoma were higher than that of fibrous root, where as the contents of Na,Al,Ca,Ge,P,Zn and Sr elements in rhizoma were lowere than that of fibrous. Both contained As,Hg,Pb and Cd elements. In the meantime, 16 mino acid were determined. The total content of them was 8.92% for rhizoma and 9.61% for fibrous. Cystine, cysteine, tryptophan and ornithine couldn't be detected.

Objective To explore the expression of livin and survivin and its correlation with caspase-3 and clinicopathology in breast cancer. Methods We examined paraffin-embedded archival specimens from 76 cases of malignant breast tumor and 17 cases of benign breast disease for the expression of livin,survivin and caspase-3 with immunohistochemistry. The relationship of livin and survivin expression with caspase-3 and clinicopathological characteristics was analyzed. Results The positive rates of livin and survivin protein expression were increased gradually in normal breast tissues,livin and survivin proliferating disease,preinvasive carcinoma and invasive breast carcinoma,while the caspase-3 protein expression was significantly decreased. The expression of livin and survivin was negatively correlated with that of caspase-3 in the four groups (P0.05). The expression of survivin was related to lymph node metastasis and tumor size (P0.05). Conclusion Livin and survivin may play an important role in accelerated tumor proliferation by inducing caspase-3 to inhibit apoptosis in breast carcinoma. Livin may be more correlated with carcinogenesis in young patients while survivin may promote metastasis and invasion in breast carcinoma.

Objective To observe the differential gene expression in human nasopharyngeal carcinoma (NPC) cell line with different radiosensitivity by cDNA microarray analysis. Methods A radioresistant cell line, CNE-2R, was established from a human nasopharyngeal carcinoma cells line CNE-2 by repeated X-ray irradiation. The differential gene expression of CNE-2 and CNE-2R was screened with cDNA microarray by BioStarH-141s profile gene chip. Results There were expressed 308 genes to be screened out between CNE-2R cell line with different radiosensitivity and its parental CNE-2 cell line, while 176 up-regulated genes in CNE-2R cells and 132 down-regulated genes were found. In them, there were 40 up-regulated ones and 36 down-regulated ones whose ratios were higher than 6.0 or lower than 0.1. The different genes included DNA damage- and repair-related genes; cell cycle-related and cytoskeleton-related proteins; and apoptosis-related genes. Conclusion The radioresistant CNE-2R cells were isolated from the CNE-2 cell line by repeated X-ray irradiation. The stable radioresistance is the result of co-effect by polygene and multiple factors, which provide several gene targets to sensitize the radioresistant cells for improving the radiocurability of NPC.

Objective To study the influence of oxy matrine combined with salvia mltiorrhiz on the hepatic fibrosis markers.Methods 60 patients with abnomal prameters of fibrosis level were randomly divided into two groups,30 patients of treatment group treated by salvia miltiorrhiz and oxy matrine,the control group was given liver protectiont treatment,then observed the changes of hepatic fibrosis markers:HA,LN,PCⅢ,and ⅣC.Results A significant decrease of HA,LN,PCⅢ,and ⅣC were observed in the patients treated with oxy matrine and salvia miltiorrhiz(P

The simulator testing in vitro and computational simulation of the artificial knee joint wear are important methods to evaluate the wear performance of the prosthesis in vitro and to predict the clinical performance of knee joint products. Based on the method of literature search, this paper compares the mechanical and kinematic loading input curves carried out by Chinese scholars in recent years, standard curves, and Chinese measurement curves of two typical movements of gait. Data of vitro simulator test and computational simulation model are compared, summarized, and analyzed. The results show that the measured data of motion and load cannot be directly used as the loading conditions for the simulator wear test and computational simulation. The mechanics and kinematics data of Chinese people are different from the international standards. The domestic artificial knee joint in vitro simulator wear test methods are similar but the results of different test institutions are somewhat different. The computation wear prediction research is basically synchronized with foreign countries, but the problem that the calculated wear results are lower than that in vitro test is still unsolved. The artificial knee joint wear performance evaluation system based on Chinese knee joint mechanics and kinematics data is the forward direction of the research.