Objective To observe the efficacy and safety of Shuxuetong injection combined with Edaravone in the treatment of acute cerebral infarction. Methods Seventy patients with acute cerebral infarction were randomly divided into two groups, the treatment group was given Shuxuetong injection and Edaravone, and the control group was treated by Shuxuening injection. Two groups were treated with routine therapy. ESS and ADL content changes in two groups were assessed at different point before treatment and two weeks after treatment. Results The score of ESS of two groups increased after treatment (P ＜ 0.05) ,but that in the Shuxuetong group was dramatically increased ,showing significantly different from that in the control group(P ＜0.05). ADL of the two groups improved after treatment(P ＜0.01) ,but the increase in the Shuxuetong group was significantly compared to the control group(P ＜0.05). Conclusion Shuxuetong injection combined with edaravone is an effective and safe medicine in treatment of acute cerebral infarction.

OBJECTIVE To monitor the characteristics of distribution and drug resistance of Acinetobacter baumannii in our hospital. METHODS A. baumannii isolates were collected in our hospital from Jan 2004 to Dec 2005. Antimicrobial susceptibility testing was performed by disk-diffusion method,according to the standards of NCCLS 2004. RESULTS Totally 177 strains of A. baumannii were distributed clinically in the respiratory unit as the most ones (47 strains, 26.6%), and in ICU as the next (38 strains, 21.5%); the older the age, the higher the appearing rate; the highest appearing rate was from the sputum, up to 78.1%; more than 60% of isolates were resistant to all antimicrobial agents tested except imipenem, meropenem and cefoperazone/sulbactam. However,10 pan-resistant strains were found. CONCLUSIONS With the increasing isolation rate of A. baumannii, its drug resistance increases simultaneously.

OBJECTIVE To investigate the epidemiology of fungemia and provide evidence for clinical therapy.METHODS A retrospective survey was done with the 69 cases of fungemia in our hospital from Jan 2004 to Dec 2008.RESULTS More than 65% of the patients suffered from two and more underlying diseases.Over a half of infections were developed following placement of catheters.And all of the patients had a history of antimicrobial agents use before blood culture.53(76.8%) cases were associated with Candida spp.Only 18 strains were C.albicans.The mortality rate of fungemia was 44.9%.Different Candida species had different resistance rates to antifungal agents.CONCLUSIONS Fungemia patients often have serious underlying diseases.Most fungemia cases are candidemia caused by non-C.albicans.Some fungal pathogens are resistant to fluconazole and itraconazole.

Objective To purify BMSCs by a simple Colony-Forming system and identify BMSCs in vitro.Methods BMSCs were planted at low-density ( 10/cm2 ) in the early stage of isolation,additional cells were scraped until a colony formed,secondly seeded those Colony-Forming cells at low-density.This process was repeat again when passaged cells formed another colony.This so called Colony-Forming selection was repeated several times until highly purified cells were obtained.Biological characters of BMSCs were observed.The surface antigens of BMSCs were identified by flow cytometry.The multipotentiality of BMSCs was assayed for differentiation into either osteoblasts or adipocytes.Results Homogeneous cells were obtained after BMSCs were passaged twice to thrice by Colony-Forming system.These BMSCs highly expressed positive surface antigens of BMSCs ( CD29 in 98.8％,CD90 in 98.4％ ) meanwhile seldom expressed negative surface antigens of BMSCs (CD31 in 2.6％,CD45 in 3％ ).BMSCs efficiently differentiated into osteoblasts and adipocytes.Conclusions Colony-Forming system is a simple and effective way to get highly purified BMSCs in a short time.

Objective:To establish the quality standard for Kechuanning granules. Methods: A TLC method was used for the qualitative study on the main ingredients, Radix stemonae,ephedra and bitter almond. An HPLC method was used to determine the con-tents of ephedrine hydrochloride,pseudoephedrine hydrochloride and amygdalin. The separation of targeted compounds was performed on a Tech Mate C18-ST column (250 mm × 4. 6 mm,5 μm) . The mobile phase consisted of methanol(A) and 0. 1% phosphoric acid (B) with gradient elution at a flow rate of 1. 0 ml·min-1. The detection wavelength was 207nm and the column temperature was 35℃. Results:The spots in TLC were quite clear without any interference from negative control. The linear relationship of ephedrine hydrochloride,pseudoephedrine hydrochloride and amygdalin was 1. 826-182. 600μg·ml-1( r=0. 9997),1. 848-184. 815μg·ml-1 (r=0. 9997) and 2. 981-298. 069 μg·ml-1(r=0. 9996),respectively. The average recovery was 97. 00%,97. 40% and 98. 30%(RSD=3.7%,2.5%,1.6%)(n=9),respectively. Conclusion: The method is sensitive,accurate,simple and reproducible,which can be used for the quality control of Kechuanning granules.

Marine medicine is an important part of Chinese medicine and a growth point of marine economy development.Exploration on the exact weight ratios and curative effect to expand marine medicine clinical application is a significant issue in marine medicine research at this stage. This paper presented a research supposition of“documents retrieval, extraction of marine medicine cases, establishment of case database, statistic analysis, data mining, herb pair Game Theory model establishment, herb pair usage under fixed dosage and matching, in order to explore a new idea of researching marine medicine combined with Game Theory. Through the literature retrieval and text extraction, 458 cases with the herb pair ofLong-Gu (LG) andMu-Li(ML) were received to establish the partnership game model. The iterative removals of strictly dominated strategies were adopted. Assuming that LG and ML used with an equal amount of 30 g was the best response, which reached the Nash equilibrium. The results suggested that dosages of LG and ML used equally at the amount of 30 g, 25 g, 24 g, 20 g, 18 g, 15 g were rationalizable strategies. Frequencies of using an equal amount of 30 g LG and ML was the most of mathematical statistics. LG and ML of 30 g were used equivalently to treat symptoms such as insomnia, inappetence, chills, tinnitus, physically and mentally fatigued, spontaneous sweating and night sweating, and so on with the effect of tranquilizing and constricting. The results can be validated and complemented among association rules analysis, mathematical statistics and game model. It implied that it was feasible to apply mathematical statistics and data mining in combination with Game Theory to study the marine medicine herb pair.

This study was aimed to analyze the research status of marine medicine in China. The research hot issues and feature rules were summarized in order to discuss development trends for the clinic application and further development of marine medicine in recent 20 years. The CNKI library was used as a source to retrieve literatures on marine medicine. The bibliometric methods and Cytoscape 3.1.1 were used to analyze. The results showed that 582 papers were included. A total of 260 papers were published during 2010 to 2014. And 52 papers were published annually. 266 papers in this field were funded. The papers were mostly published in theChinese Journal of Marine Drugs. A total of 278 research institutes were engaged in the research. And the institute published the most paper was the Ocean University of China. There were 12 researchers who had published more than 5 articles. This study focused on the keywords with highly frequency, which were marine drugs, chemical composition, marine natural products, fingerprints and antitumor. It was concluded that marine medicine had become hot issues. The core of the group has been formed. However, most researches had focused on natural products and pharmacological aspects. Traditional Chinese medicine (TCM) theory had not been taken seriously and the infrastructure was weak, which obstructed the clinical application of marine medicine. We should expand the discovery and study of knowledge on ancient herbal literature as well as clinic application of marine medicine in order to build the knowledge database of marine medicine and experience mode of clinic application.

OBJECTIVE To analyze three methods with five antifungals susceptibility test.METHODS Eighty(pathogenic) yeasts isolated from various specimens were detected by dish diffusion method for yeast susceptibility testing in three different agars [Shadomy′s modified agar,RPMI 1640 agar,M-H(GMB)agar]with comparison of the Shadomy′s modified agar.RESULTS According to sensibility of five antifungals,RPMI 1640 was the same with Shadomy,but GMB M-H was lower with others.Three different agars had more correlability(P

AIM:To observe the expressions of cathepsin B (CB) and cystatin C (CC) in different stage of diabetic rats and to investigates their potential roles.METHODS:Sixty rats were divided into diabetes mellitus group induced by intravenous injection of streptozotocin (55 mg/kg) and normal group injected with citrate buffer. Ten rats were sacrificed respectively at the end of fourth week,eighth week and sixteenth week in both groups. 24 h urine excretion was collected in rats before sacrifice. The blood and the kidney were also collected. The mRNA and protein expressions of CB and CC in kidney were detected by real time PCR and immunohistochemical staining,respectively.RESULTS:At the end of eighth week,the expression of Ccr,24 h urinary protein excretion,CB,CC in diabetic rats increased significantly,compared to the results at the fourth week (P

Objective To describe X-ray and CT findings of lung in paraquat poisoning.Methods CT and X-ray data of chest in 21 patients with paraquat poisoning were retrospectively analyzed.Results X-ray and CT findings of lung were varied with different time.≤7 d,there were mainly increase of lung marking in 17 cases,ground-grass attenuation in 14 cases and consolidation in 2 cases.7~14 d,increase of lung marking,consolidation and pulmonary fibrosis each could be seen in 4 cases,and 3 cases with ground-grass attenuation.≥14 d,there were pulmonary fibrosis in 4 cases,bronchiectasis in 3 cases,increase of lung marking in 2 cases.Conclusion Chest plain X-ray film and CT are of important value in observing the evolution,evaluating the prognosis and guiding the treatment project for the patients with paraquat poisoning.