OBJECTIVE:To observe clinical efficacy of clomiphene combined with metformin in the treatment of polycys-tic ovary syndrome. METHODS:112 patients with polycystic ovary syndrome admitted in gynaecology and obstetrics depart-ment of our hospital were selected,and randomly divided into control group and observation group,with 56 cases in each group. Control group was treated with clomiphene orally,since fifth day of menstruation,once a day,50 mg/time;observa-tion group was additionally treated with metformin orally,30 min before meal,3 times a day,500 mg/d. Treatment course last-ed for 2 months. Biochemical indicators,control time of disease,actual treatment time,HOMA-IR,HOMA-β and clinical effi-cacy of patients were observed in two groups. RESULTS:After treatment,LH,FSH,T and FINS of patients in observation group were lower than before treatment and control group,with statistical significance(P<0.05);control time of disease and actual treatment time of observation group were significantly shorter control group (P<0.05),with statistical significance;HOMA-IR and HOMA-β of observation group were significantly lower than those of control group,with statistical significance (P<0.05);clinical effective rate of patients in observation group was significantly higher than control group,with statistical significance (P<0.05). CONCLUSIONS:Clomiphene combined with metformin in the treatment of polycystic ovarian syn-drome can improve biochemical indicators,shorten control time of disease and actual treatment time and enhance clinical effi-cacy.

Starting on the research findings from a study on cancer patients decision making,the author tried to further analyze the Doctor-Patient-Relationship in China from the historical perspective.Having the disease re-moved or treat the patient responsibly,represents different meaning and implication on how a society regards the medicine as profession,and how define the role of physicians.Considerations on these issues are hoped to facilitate the improvement of doctor-patient relationship in China.

Objective:To construct a prokaryotic expression plasmid containing HCVF gene,and purify the recombinant fusion protein in E.coli system. Methods: F gene of Hepatitis C virus was amplified by PCR method from plasmid H/FL(containing full length cDNA sequence of HCV 1a subtype),cloned into pET32a(+)vector,and then transformed into E.coli JM109. After identified by restriction diges- tion and DNA sequencing,recombinant plasmid was transformed into E.coli BL21 and induced with IPTG. The fusion protein trxA- F was further confirmed by Western blot analysis and purified by affinity chromatography method. Results: Restriction digestion and PCR screening showed that HCV F gene was cloned into pET32a(+)successfully. After BL21 was transformed with recombinant vector pET32a (+)- HCVF and induced with 0.5mmol/L IPTG,a 35.4kDprotein band was found bySDS- PAGE. And this recombinant protein showed a highly specific and strong reaction with anti- His monoclonal antibody by Western blot analysis. Conclusion: The prokaryotic expression plasmid pET32a(+)- HCVF was successfully constructed,which will be helpful for further research.

Objective:To investigate the changes of reactive oxygen species(ROS)and Glutathione(GSH) and their chronological order in the apoptosis of K562 cells induced by simvastatin to explore the apoptosis mechanism.Methods:K562 cells cultivated in routine method for 24h were exposed in the simvastatin 20?mol/L.Cell morphological analysis were conducted at 48h and the vitalities of K562 cells were cultivated detected with MTT at different times(12,24,48,72 h) respectively.Fluorochrome flow cytometry was performed to detect cell apoptotic ratio and changes of intracellular ROS.The content of intracellular GSH in K562 cells was measured by colorimetric method.Results:K562 cells presented typical apoptosis morphological changes the treatment of simvastatin for 48h.cell inhibition ratio at different times(12,24,48,and 72 h) were 0,(19.02?0.92)%,(56.4?3.20)%,(74.4?5.54)% respectively and cell growth of K562 was inhibited.The apoptotic ratio were(2.55?0.25)%,(6.1?0.35)%,(14.15?0.42)%,and(30.70?0.65)% respectively.Furthermore,the changes of fluorescence intensity of ROS were also significantly increased(P

Based on the peak value of power spectral density (PPSD) and corresponding frequency (CF), an approach that performs electroencephalogram (EEG) feature extraction during imaginary right and left hand movements was proposed. The data were gained from brain computer interface competition in 2003 provided by Graz University of Technology. The EEG signals between 8-16 Hz were decomposed by db3 wavelet packet at three levels. The PPSD and CF of electrodes C3 and C4 were defined as the EEG feature vectors and calculated respectively. The left and right hand motor imaginary tasks were distinguished by the time-variable linear classifier. The proposed method was applied to the test data for 140 trials. The satisfactory results were obtained with the highest classification accuracy 89.29%. The maximum mutual information was 0.622 8 bit, and the signal-to-noise ratio (SNR) was 1.371 3. The PPSD and its CF on electrodes C3 and C4 between 8 and 16 Hz were coincident with event-related desynchronization (ERD) and event-related synchronization (ERS). This method is simple, quick, and promising for on-line brain computer interface system.

Objective To investigate postpartum blood lose of cesarean section (CS) within 24 hrs in Nanjing area Methods Stratified samples were collected from hospitals of different levels and located at different districts The amount of postpartum blood loss was precisely measured by methods of weight, volume and area Results There were 1 125 CS in a total of 4 171 deliveries The main indications for CS were cephalo pelvic disproportion, fetal distress, breech presentation, and there was 10 4% of CS without definite causes The average amount of blood loss was 520 ml in CS, which was far more than that in vaginal delivery ( n = 3 046) If postpartum hemorrhage (PPH) is defined as blood lose of 500ml or more, the incidence of PPH was 53 7%; if it is defined as 700 ml, the incidence was 19 8% Conclusions Cesarean section is an important cause of PPH, and CS without proper indication should be avoided It seems reasonable to defined the criteria of PPH in CS as 700 ml or more

Traced back to the one decade's development of research ethics in China,the author summarizes the current issues,such as: not sufficient implementing of informed consent,relatively loose review of IRB,IRBs lack ability to improve the review level and not provide much education opportunities to researchers,and not yet take account into the continuous review to the protocols approved before.By comparing the current issues of China and the conditions of U.S.in 1998,we can find some similarities exist,so we can learn from the lessons and experiences of the development of research ethics in the United and the other country,such as adding the model of region ethical review committee,instead of only institutional IRB.The author aims to point out some general ideas about research ethics capacity building,enforce training continuously,government pay much attention to IRB regulation and consider much on cultural elements,which are all bases for the road of sustainable development of research ethics in China.

OBJECTIVE:To observe the changes of mitochondrial membrane potential(MMP)at different time in the process of simvastatin-induced apoptosis of K562 cells.METHODS:K562 cells cultivated by routine method for 24h were treated in the 20? mol? L-1 simvastatin for 12,24,48,and 72h,respectively.And theh cell morphological changes were observed.Vitality of K562 cells was detected with MTT;flow cytometry were performed to detect cell apoptotic ratio and changes of mitochondrial membrane potential,and the detection results were compared with those in solvent control group.RESULTS:As compared with control group,the morphology changes of K562 cells such as karyopyconosis,nuclear fragmentation and apoptotic body emerged after treatment with 20? mol? L-1 simvastatin for 48h;the apoptotic ratio at 12,24,48,and 72h increased by(2.55? 0.35)%,(6.1? 0.35)%,(14.15? 0.42)%,and(30.70? 0.65)%,respectively;and the apoptotic ratio of K562 cells increased time-dependently.The cell membrane potential was decreased by(0.7? 0.24)%,(39.6? 4.80)%,(24.4? 2.45)%,and(6.0? 1.62)%,respectively,with that at 24h showing the highest decreasing rate.CONCLUSION:The change of MMP is the early events of apoptosis.The potential mechanism of K562 cells apoptosis induced by simvastatin might be related to the collapse of MMP.

The results of the arterial blood study in 30 patients of cor pulmonale in acute exacerbation were reported.The study included gas analysis, and dominations of serum lactate,pyruvate, potassium,sodium and chlorine.All the items were determined simultaneously.The data demonstrated that when the arterial oxygen tension of the patients with cor pulmonale in acute exacerbation dropped below 60mmHg, the blood lactate concentration was most likely to show a rise, which was related not only to hypoxemia but also to alkalosis.Observations of the changes in concentration of blood lactate and pyruvate in some patients led us to believe that these changes were associated with the seriousness of the disease. Regular measurement of blood lactate and pyruvate concentrations might be helpful to judge the condition of the patient. Persistent rise in blood lactate concentraion suggested poor prognosis.