Anomalous coronary artery origin includes a variety of congenital coronary vascular variations. The left main coronary artery opening in the right coronary sinus is a rare and serious coronary artery malformation. The symptoms of angina, syncope and myocardial ischemia were found in this patient, but some patients showed no clinical symptoms and died suddenly after strenuous exercise. To improve the understanding of the disease early, the early surgery is the key to improve the prognosis.

Objective To study the effect of different personality on procrastination behavior of university students.Methods 126 university students were assessed by the General Procrastination Scale (GPS) and the big five personality inventory short form(NEO-FFI).Results GPS score of the student was(53.26±9.92).There were 84.9％ students respectively had different level of procrastination behavior.There were no significant differences in university students by gender and grades.There were significant negative correlation between procrastination and personality factor of conscientiousness (r=-0.563,P＜0.01) and significant negative correlation with agreeableness (r=-0.236,P＜0.01) and extraversion.The big five personality factors of conscientiousness can effectively predict the procrastination behavior of college students.Conclusion Procrastination is a very common phenomenon in the university students.The students with lower conscientiousness,agreeableness and extraversion of big five personality have more procrastination behavior.

Objective To study the mechanism of tumor necrosis factor(TNF) ? in psoriasis vulgaris. Methods The expressions of 55 kD(p55) and 75 kD(p75) TNF receptors, intercellular adhesion molecule(ICAM) 1, lymphocyte function associated antigen(LFA) 1 in the lesional skin of psoriasis vulgaris were detected by immunohistochemical method. Results Normal skin did not express p55, p75, ICAM 1 and LFA 1, the epidermis of the lesional skin expressed p55, p75 and ICAM 1, the infiltrating mononuclear cells (MNCs) in the dermis expressed p55, p75 and LFA 1 in psoriasis vulgaris. The intensities of staining of the p55 and ICAM 1 on epidermal keratinocytes(EKC) were significantly correlated (r' s=0.704, P0.05). The staining intensity of ICAM 1 on EKC and the number of infiltrating MNCs in the dermis was significantly correlated (r' s=0.724, P

Objective:To study the effect of astragalus polysacchrides on anticardiolipin, antiphosphatidyl choline, antiphosphatidyl serine, antiphospha-tidyl inositol, antiphosphatidic acid and antiphosphatidyl ethanolamine antibodies (aCL, aPC, aPS, aPI, aPA, aPE) in SLE mice. Methods:19 NZB?NZW F1 female mice were divided randomly into 3 groups: group PG2I (25 mg/kg/d), group PG2II (50 mg/kg/d), group NS (0.2 ml/d). Select BXSB and C57BL/6 female mice as control. The level of antiphospholipid antibodies in these mice were examined by ELISA. Results:Compared with group NS, the A value in group PG2II were decreased significantly, however, A value in group PG2I were elevated slightly. There were no difference among group PG2II, BXSB and C57BL/6. The level of antiphospholipid antibodies in group NS and PG2I were increased significantly compared with that of BXSB and C57BL/6 mice. Conclusion:The generation of antiphospholipid antibodies can be improved by low dose of PG2 and inhibited by high dose of PG2.

ObjectiveTo analyze the distribution and density of Langerhans cells and dermal CD68 positive histiocytes in lesions of secondary keloid.MethodsTissue specimens were resected from the lesions of 30 patients with secondary keloid and normal skin of 14 human controls.Immunohistochemistry was performed to observe the expressions of CD68 and CD1a in these specimens.A micrometer was used to count the number of positively stained cells per unit area.The Student's t test was conducted for data analysis by using the SPSS software.ResultsThe density of CD1a+ Langerhans cells was (61 ± 49) cells/mm2 in the epidermis of secondary keloid lesions, (258 ± 61 ) cells/mm2 in the control epidermis,and(40 ± 65) cells/mm2 in the dermis of keloid lesions.CD68+ cells were absent in the epidermis of keloid lesions.Significant differences were observed in the density of CD1a+ Langerhans cells between the lesional and normal control epidermis(t =9.88,P ＜ 0.001 ) and in the percentage of CD68+ cells in nucleated cells between the superficial dermis of lesions and control skin(62％ ± 12％ vs.70％ ± 14％,t =2.66,P ＜ 0.05).The density of dermal CD68+ histiocytes was similar between the lesions and control skin ((287 ± 73) cells/mm2 vs.(290 ± 22) cell/mm2,t =0.02,P ＞ 0.05).Conclusions In keloid lesions,Langerhans cells decrease in the epidermis but increase in the dermis,CD68+ histiocytes are absent in the epidermis,and reduced in the dermis with a declined percentage in nucleated cells.

Objective To study the changes in some important surface markers of Langerhans cell (LC) in the skin of SLE patients. Methods The normal-appearing skin and lesional skin of 9 SLE patients were studied. ABC immunohistochemistry and the monoclonal antibodies against HLA-DR, CD54, CD68, CD1a and CD4 were used. Results ①In SLE skin lesions decreased LC density was shown,and there were also changes in the morphology and surface markers of LC. ②HLA-DR expression on keratinocytes was shown in most lesional specimens and a few on non-lesional specimens. ③Neither CD4 nor CD54 expression was shown on both lesional epidermis and non-lesional epidermis, CD4+cells were only observed in the dermal infiltrates. ④Two types of CD68+dendritic cells were seen in lesional and non-lesional epidermis, and more CD68+dendritic cells were seen in the infiltrates of lesional skin. ⑤Fibrillar CD68+materials around the basal KC were observed, and some of such fibrillar materials were connected with epidermal dendritic cells while others were not. Conclusions There are some differences in LC surface marker expression on lesional skin and non-lesional skin of SLE patients, which need to be further studied.

Objective To observe the effects of different dosages of granulocyte-macrophage colony-stimulating factor (GM-CSF) on generating the routine bone marrow dendritic cells, and supply suitable dosage of GM-CSF on preparation of dendritic cell vaccines used for different purpose. Methods Using low (5 ng/ml) and conventional (20 ng/ml) and high dosage( 50 ng/ml ) of GM-CSF combined interleukin-4 ( IL-4 ) to induce murine bone marrow dendritic cells were performed, The phenotypes (CD_(11c), CD_(80), CD_(86)) and functional properties of the DC were compared by FACS analysis and MLR. Results The DC induced by low dosage of GM-CSF were immature DC, expressing low CD_(11c), CD_(80), and CD_(86). DC induced by conventional dosage were functional mature, expressing higher CD_(11c), CD_(80), CD_(86), which could induce allogeneic T lymphocyte responses. DC induced by the high dosage GM-CSF were the most phetotypicaUy and functional mature cells, expressing the highest CD_(11c), CD_(80) CD_(86), which could induce the strongest allogeneic T lymphocyte responses. Conclusion The dosages of GM-CSF affect the maturation stage of dendritic cells. Low dosage of GM-CSF generated immature dendritic cells, but conventional dosage and high dosage generated mature dendritic cells. DC generated through high dosage of GM-CSF were the most mature in phenotype and function.

Objective To observe the therapeutic effect of rhubarb combined with Probiotics for treatment of patients with severe acute pancreatitis (SAP) accompanied by acute lung injury (ALI). Methods A prospective randomized controlled trial was conducted. Sixty patients with SAP and ALI were randomly divided into a rhubarb group and a combination of rhubarb and Probiotics group (combined group), 30 cases in each group. Both groups were treated with conventional therapy. On that therapeutic base, the patients in rhubarb group were treated with rhubarb 3 g·kg-1·d-1 (Raw rhubarb in boiling water 200 mL was cooked for 30 minutes, then the decoction was divided into two parts to be infused through nasal jejunal tube, retaining for 1 hour);while in the combined group, beside the above rhubarb therapy, the patients were additionally given Probiotics [Clostridium butyric acid:Chang Lekang capsule, once 1 grain (0.42 g), twice a day] through nasal jejunal tube. The therapeutic course was 14 days in the two groups. The plasma endotoxin levels, oxygenation index, and acute physiology and chronic health evaluationⅡ(APACHEⅡ) score were observed before and after treatment for 7 days and 14 days, respectively. After treatment for 30 days, the time of intestine functional recovery and mechanical ventilation, number of damaged organs, number of infectious sites, number of pancreatic pseudocysts, length of intensive care unit (ICU) stay and 30-day fatality rate were observed in the two groups. Results With the prolongation of treatment, the plasma level of endotoxin and APACHEⅡscore in the two groups were gradually reduced, and the oxygenation index was gradually increased after treatment for 7 days and 14 days in the two groups compared with those before treatment, and there were statistically significant differences in above indexes on the 14th day after treatment between combined group and rhubarb group [endotoxin (μg/L): 19.16±1.90 vs. 21.20±1.05, oxygenation index (mmHg, 1 mmHg = 0.133 kPa): 369.50±5.45 vs. 341.50±7.36, APACHE Ⅱscore:11.84±0.60 vs. 13.00±0.86, P<0.05 or P<0.01]. The length of intestine functional recovery in the combined group was longer than that in the rhubarb group (days: 5.63±1.75 vs. 5.02±1.54), the number of damaged organs (2.08±0.74 vs. 2.70±1.11), the number of infectious sites (1.93±0.64 vs. 2.23±0.83), the number of pancreatic pseudocysts (1.22±0.34 vs. 1.63±0.11) and the mortality rate [10.0%(3/30) vs. 13.3%(4/30)] in the combined group were lower than those of the rhubarb group, but there were no statistically significant differences between two groups (all P > 0.05). The times of mechanical ventilation (days: 13.40±1.76 vs. 15.60±1.28) and the length of ICU stay (days:16.13±1.25 vs. 17.63±1.30) were significantly shorter in combined group than those in rhubarb group (both P<0.05). Conclusion Rhubarb combined with Probiotics for treatment of patients with SAP and ALI can promote their recovery of lung function and shorten their length of ICU stay.

Aim To investigate the protective effect of ginsenoside Rb1 against apoptosis induced by H_2O_2. Methods H_2O_2 was used to build an oxidative stress-induced injury model in neonatal rat cardiomyocytes. After treated with gensenoside Rb1(20, 40, 80 mg?L -1),the apoptosis rate, the content of malondialdehyde (MDA), and the activity of superoxide dimutase (SOD) of the cardiomyocytes were examined. The intracellular calcium indicated by the fluorescence in cells were measured by the laser confocal microscope. Results Compared with the model group, the apoptosis rate and the content of MDA of the cardiomyocytes decreased greatly (P

Objective:To observe the distribution,morphology and density of monocytes/macrophages and dendritic cells in the normal human dermis.Methods:Normal skin from 6 locations such as the face,trunk,proximal limbs,distal limbs,and palms and soles of 8 subjects were collected for the study.The horizontal and longitudinal sections of the skin were stained with an ABC immunoperoxidase procedure with anti-CD1a and anti-CD68 monoclonal antibodies.Results:In the superficial dermis CD68 positive monocytes/macrophages form a dense network with a density in a 6-micron section ranging from 361/mm~2 to 562/mm~2.These network of CD68 positive cells continued on to surround the blood vessels and skin appendages.Lower densities of CD68 positive dendritic cells were found in the deep(reticular) dermis,dispersed between collagen bundles.The CD68 positive cells were detected within the superficial dermis with variable densities: distal limbs 562/mm~2,trunk 517/mm~2,face 509/mm~2,palms 507/mm~2,proximal limbs,472/mm~2,and soles 361/mm~2.Conclusion:There exists in the superficial dermis a relatively dense network of CD68 positive monocytes/macrophages.Such a distribution might indicate the clear polarity of the dermal monocytes/macrophages,with their direction of defense towards to the dermal-epidermal junction.