anti p62 2 in patients with cancers All of them were lower than that of autoantibody against full length p62 The frequency of autoantibody to full length p62 was significantly higher than that of anti p62 2 and anti p62 3, but no difference with anti p62 1 Totally positive rates of autoantibody against the three fragments were lower than those of full length p62 in patients with hepatocellular cancer, gastric cancer, colorectal cancer, esophagus cancer and breast cancer but with lung cancer Conclusion:Antigenic sites in p62 were mainly located in p62 1 and the integrity of p62 structure is important for antigenic expression

extensive injury group.The intelligence quotient level of left frontal lobe group was lower than that of the right;PIQ of right temporal lobe group was lower than that of the left,VIQ of right temporal lobe group was higher than that of the left(P

Objective To evaluate the clinical effect of CYPHER TM (rapamycin) stent in treatment of left anterior descending (LAD) long lesion. Methods From October 2002 to April 2003, 65 patients with coronary heart disease (CAD) were treated with CYPHER TM (Cordis) stent and 65 stents were planted in 53 LAD lesions. Among the patients with LAD lesions, 42 cases were long lesions (≥20 mm). The length of lesion was from 20 mm to 50 mm (28.2?8.8 mm), the degree of stenosis before procedure was 88.9%?8.5%, and the vessel diameter before procedure was 3.0?0.9 mm. Three lesions were chronic total occlusion (CTO) lesion. Two lesions were in-stent restenosis. 12 lesions over 35 mm long were treated with two overlapped stents to cover the whole lesion. 30 lesions were treated with only one stent. We observed the procedure success and complication rates. The occurance of angina, myocardial infarction, sudden death, and the revascularization rate during the in-hospital and 6 months follow up. Results Immediate procedure success rate was 100% with residual stenosis of 5.0%?4.8%. All the lesions were covered fully by stents and the coronary flow reached TIMI grade three. No new dissection was observed in the optimal of stents. Only one patient was diagnosed as myocardial infarction after procedure and in-stent thrombolysis was proved by coronary artery angiography (CAG). During the 6 months follow-up, the symptom of angina was disappeared in 38 patients and alleviated in 4 patients. Eight patients received CAG in 3-6 months and no restenosis was observed. Conclusion Drug eluting stent (CYPHER TM) implantation is a safe and efficient therapy in treating patients with LAD long lesion, and short term follow-up results were better than the general coronary stents.

Objective To investigate the expression and its rule of transferrin (Tf) in brain tissue at different time after experimental intracerebral hemorrhage (ICH) in rats. Methods The fresh quantitative autologous blood was infused into the right caudate nucleus of rat sterotaxically to build up experimental ICH model. At 6h, 24h, 72h and 7d after operation, the rats were sacrificed and the brain tissues were made for immunohistochemistry analysis of Tf. The water content of the brains was also assayed.Results Compared with saline group, Tf-immunostaining positive cells in the tissue surrounding hematoma and in the ipsilateral pallium in ICH rats were increased significantly during 7 days, peaked at 72 hours ( P

Objective To investigate the expression of neuron nitric oxide synthase (nNOS) mRNA of brain tissue at early stage of intracerebral hemorrhage (ICH) in rats and the intervention effect of Didangtang.Methods 72 male Sprague-Dawley rats were randomly divided into four groups: intracerebral hemorrhage group, Didangtang treatment group, NOS inhibition group and normal saline group. Quantitate fresh autologous blood was infused into right caudate nucleus of rat sterotaxically to build up experimental ICH model and normal saline was instead in control group. The neurological function deficit scores were observed by Bederson method (3 grades) at 6 h, 24 h and 72 h after operation. At the same time points, the rats were sacrificed and the brain tissues were taken out for the measurement of nNOS mRNA by technique of hybridization in situ.Results Neurological function deficit scores of the rats were significantly improved both in intracerebral hemorrhage group and Didangtang treatment group at 72 h after operation(all P

Objective To evaluate the clinical diagnostic role of dental computed tomography (CT) and three-dimensional reconstruction (3DR) compared with conventional dental periapical radiography (DPR) in the diagnosis of molar furcation involvement (FI). Methods Dental CT and 3DR were performed about a maxillary first molar and its alveolar bone diagnosed as FI II? by conventional probe technique, and the images were compared with that of DPR. Results The images of dental CT scans and 3DR showed not only the area, type and quantity of periodontal destruction, but also the complex stereoscopic anatomical vision and ambient spatial relationship around the molar. Especially, the images displayed the alveolar destructions of the dental buccal and palate site that could not always be demonstrated by DPR. The degree of the molar FI was defined as III? in fact. Conclusion Dental CT and 3DR have many superiorities and value, which can not be replaced by DPR, in the clinical diagnosis and prognostic judgment of molar furcation involvement.

Objective:To study the effect of quercetin given during pregnancy and lactation period of obese rats on weight and expression of insulin-like growth factors-1 ( IGF-1 ) mRNA of the offspring . Methods:In the study , 8 healthy weaning female SD rats were randomly selected to feed the basal diet and as blank control group , and the others were fed with high-fat diet.When the average weight of the high-fat diet rats was 20% as many as the average weight of the control group , we believed the model succeeded .Then the female SD rats were mated and the pregnant rats were randomly divided into 5 groups , 8 rats in each .They were fed with high fat diet during pregnancy and lactation , and the obese pregnant rats were respectively irrigated with 0 mg/kg body weight ( high-fat control ) , 50 mg/kg body weight,100 mg/kg body weight , and 200 mg/kg body weight quercetin .The blank control group was fed with basic diet throughout the experimental period .The birth weight , the weights during the development of d7, d21, d56, and d98, and the expressions of IGF-1 mRNA in livers tissue of the F1 generation were measured.Results:The average birth weight of the F1 generation of high fat diet (HFD) group was sig-nificantly higher than that of the blank control group .Decreased offspring birth weight was observed with the intervention of quercetin , which effect remained to the delectation .The quercetin also reduced the ex-pression of IGF-1 mRNA in livers of the F1 generation , and this effect remained to the adulthood .The 200 mg/kg body weight quercetin was most significantly effective .Conclusion:The averaged birth weight of the F1 generation of obese pregnant rats was significantly increased , and quercetin could effectively in-hibit the expression of IGF-1 mRNA in livers of F1 generation to decrease cell proliferation and cell differentiation.

Objective To investigate the treatment mechanism and effect of platelet-rich plasma(PRP) in treating rabbit knee osteoarthosis(KOA).Methods Thirty-two New Zealand adult clean class rabbits were randomly divided into the blank control group(A),model control group(B),PRP treatment group(C) and sodium hyaluronate treatment group(D).The group A conducted the simulated model construction,while the group B,C and D established the KOA rabbit model by using the Hulth method.After establishing the animal model,the group C was given PRP 0.5mLl by knee articular cavity injection,once every 3 weeks,twice in total;the group D was given sodium hyaluronate by knee articular cavity injection,once per week for 5 continuous weeks.The group A and B were injected with equal amount of normal saline at the same time point in the group C.The histological structure of articular cartilage,cell number,integrity of tidal line,and toluidine blue staining were observed by common optical microscope.The differences of Mankin′s scores were compared among various groups.The proper amount of knee joint fluid was collected for measuring the arachidonic acid expression in each group.Results The structure and morphology of articular cartilage in the group A were normal without obvious damage,while which in the group B,C and D had different degrees of damage,especially the cartilage structure in the group B had greater changes.Although the group B and C had the morphological and structure change of articular cartilage,but which was close to the cartilage structure in the group A.The Mankin′s score in the group A was lowest,while which in the group B was highest,which in the group C and D was significantly decreased after intervention treatment;the difference between the group B with the group C and D had statistical significance(P<0.05).The difference between the group C and D had statistical significance(P<0.05).The arachidonic acid level in the group A was lowest and which in the group B was highest,which in the group C and group D ranged between the group A and B,moreover the group C was lower than the group D (P<0.05).Conclusion PRP has obvious therapeutic and alleviated effect in treating KOA.

OBJECTIVE:To preliminarily study the stability of 3 pieces of Chinese medicinal formula(CMF)after decoction, and provide reference for guaranteeing storage quality of decocted liquid and improving safety of drug use. METHODS:3 represen-tative formulas of Gegen Huangqin Huanglian decoction(A formula),Wuling powder(B formula)and Didang decoction(C for-mula)from Shanghan Zabing Lun were selected,the decocted liquid were stored under ambient temperature(25 ℃)and refrigerat-ed temperature (4 ℃) after decocting by automatic boiling-machine and packing. The microorganism,precipitation,pH and con-tents of total flavonoids,alkaloid,polysaccharide,total protein after 1,7,14,21,28 d were detected. RESULTS:Compared with the first day,contents of total flavonoids,polysaccharide in formula A at ambient temperature group were significantly in-creased on the 28th(P<0.05),content of polysaccharide in refrigerated temperature group was significantly increased(P<0.05). Content of polysaccharide in formula B at ambient temperature group was significantly decreased(P<0.05). The pH and content of total flavonoids in formula C at ambient temperature group and refrigerated temperature group were significantly increased (P<0.05 or P<0.01). Other indexes showed no obvious changes during the trial period. CONCLUSIONS:Under ambient temperature and refrigerated temperature,liquid ingredients of above decocted CMF will change when storing for 4 weeks. It indicates that the storage time of decocted CMF should not be more than 3 weeks.

Objective To identify the specific proteins in two hydrophis (l#and 2#) with a simple differential proteins display method. Method By means of SDS-PAGE and Western blot, combined with analysis, the specific proteins in two hydrophis were obtained and been sequencing.Result The specific proteins of l#are 14.6kD and 8.7kD components and of 2#are 16.7kD. 10.2 kD components. The amino acid sequences of 14.6 kD and 10.2 kD components were assayed respectively. Conclusion Differential proteins can be obtained and used to identify the Chinese traditional medicinal crops by means of one-dimensional electrophoresis combined with antigen analysis .