Forty-three cases of malignant tumors with metastases to serous cavites were treated by interventional chemotherapy under ultrasound guidance and monitoring. 43 cases (83 injections) were all successfully operated and followed up. After peritoneoclysis, besides the common side effects of chemotherapyie nausea and vomiting, 3. 8% of the patients had abdominal pain and 75. 5% had abdominal distention. No complication of hemorrhage, infection, drug leakage or organ injury oceurred. It is suggested the method is safe easily to be used especially for the intracavitary chemotherapy of malignaant tumors and deserves to be widely employed.

Objective To investigate the association of serum adiponectin and tumor necrosis factor alpha (TNF-α) with endothelial damages in metabolic syndrome (MS) patients. Methods One-hundred and five patients diagnosed as MS were recruited as cases and 37 subjects without characteristics of MS were recruited as controls. The cases were divided into two groups according to the level of serum von Willebrand factor (vWF) :normal endo-thelial functional MS group (57 patients) and abnormal endothelial functional MS group (48 patients). Serum adi-ponectin concentration was measured by enzyme-linked immanosorbont assay (ELISA). Serum TNF-α concentration was measured by radioimmunoassay (RIA). Results The concentration of serum adiponectin in normal subjects (10.5 mg/L [SD:3.2]) was significantly higher than both that of normal endothelial functional MS group (7.9 mg/L [SD :2.2]) and abnormal endothelial functional MS group (6.5 μg/L [SD :2.5]) (P < 0.05). The concen-tration of serum TNF-α in normal subjects(0.17 μg/L [SD :0.04]) were significantly lower than that of normal en-dothelial functional MS group (0.19 μg/L [SD:0.05]) and abnormal endothelial functional MS group (0.23 ng/mi [SD: 0.06]) (P< 0.05). The level of serum adiponectin were negatively correlated with TNF-α(r=- 0.555, P < 0.01). Logistic regression analysis results showed that high level of TNF-α was a risk factor of endo-thelial damages (OR = 20.649, P = 0.035), whereas high level of serum adiponectin protected against endothelial damages (OR = 0.340,P=0.006). Conclusions Low level of serum adiponectin and high level of serum TNF-α may correlate with endothelial damages in MS patients.

18F-2-fluro-D-deoxy-glucose (18F-FDG) is used by positron emission tomography/computed tomography (PET/CT) examination as an imaging agent,by observing the uptake of the agent of the lesion to determine its metabolism,and make a diagnosis.It can provide anatomical and functional at the same time,not only qualitative but also position and can significantly improve the accuracy of diagnosis.Currently PET/CT has been widely used in the clinical aspects of the differential diagnosis of benign and malignant tumors,prognostic assessment and so on.The property of pleural effusion is important to the treatment and prognosis of the disease.

Objective To explore the clinical characteristics and the levels of interleukin-17 (IL-17) in Mycoplasma pneumoniae (MP) pneumonia patients with atopic sensitization.Methods One hundred and sixty-two patients with MP pneumonia were hospitalized at the Department of Pediatrics,Tianjin Nankai Hospital from October 2015 to December 2016.All children with MP pneumonia were evaluated and divided into 2 groups:the atopic group (atopic MP pneumonia children,n =50) and the non-atopic group (non-atopic MP pneumonia children,n =112).Furthermore,30 healthy children were also included in this study as healthy control group at the Department of Pediatrics,Tianjin Nankai Hospital at the same time.The clinical characteristics and laboratory data of MP pneumonia patients of 2 groups were recorded and analyzed.The levels of serum IL-17 of MP pneumonia patients were measured by using enzyme-linked immunosorbent assays.Results Children with atopic MP pneumonia had more severe pneumonia.At the same time,the number of cases of oxygen therapy and glucocorticoid treatment,the duration of glucocorticoid treatment and the history of previous asthma in the atopic group were higher than those in the non-atopic group,and there was statistical difference between 2 groups(all P ＜ 0.05).The level of serum total immunoglobulin E in the atopic group [230.5 (120.8,421.5) IU/mL] was higher than that in the non-atopic group [79.9 (46.1,125.3) IU/mL],and the level of serum lactate dehydrogenase(LDH) in the atopic group [(319.3 ± 118.9) IU/L] was higher than that in the non-atopic group [(255.5 ± 66.6) IU/L],and there were statistical differences between 2 groups (all P ＜0.01).The levels of serum IL-17 in the healthy control group,atopic group and non-atopic group were (60.2 ± 15.7) ng/L,(382.2 ± 181.7) ng/L and(532.3 ± 169.1) ng/L,respectively,and there were statistical differences among the 3 groups (F =105.668,P =0.000).Whereas,the level of serum IL-17 in the atopic group was lower than that in the non-atopic group,there was statistical difference between 2 groups (t =-3.861,P ＜ 0.01).Conclusion Atopy mav cause adverse effects on the childhood MP pneumonia.It was likely to be relevant to low levels of IL-17.

Objective To investigate the influence of intravenous infusion by double-lumen peripherally inserted central catheter (PICC)on continuous dynamic central venous pressure(CVP)monitoring.Methods In the patient undergoing the double-lemon PICC,continuous dynamic CVP monitoring was performed by a lumen and the intravenous infusion was conducted by another lu-men.Whether the simultaneous venous infusion affecting the values of continuous dynamic CVP monitoring was observed.Results Intravenous infusion through double-lumen PICC had no influence on simultaneously continuous dynamic CVP monitoring (P>0.05),which could influence the values of CVP monitoring when simultaneously using the intravenous infusion pump(P<0.05). The speed of pumping fluid had no influence in a shout time(10 min),with significant difference(P>0.05).The CVP values could recovered to the status before infusion when continuously infusing after stopping using the infusion pump.Compared with before in-fusion,the CVP values after stopping intravenous infusion had no obvious change(P>0.05).Conclusion Intravenous infusion by double-lumen PICC has no influence on continuous dynamic CVP monitoring.But simultaneously adding infusion pump has certain influence on the CVP monitoring values.

Objective To study the repairment of pancreas mesenchymal stem cells(MSCs) on injured pancreas of rats,and find a new source of cells for treatment of diabetes. Methods ① Pancreases were taken out of three-day-old Wistar rats under bioclean condition,and cells were obtained by V-collagenase digestion. Generations were passed by conventional culture,cells were purified by adherence screening method,cell morphous was observed by Giemsa staining. The expressions of surface marker CD34 and CD44 were determined by FCM,and compared with bone marrow MSCs(BM-MSCs),the differences in character and function were observed. ②The experimental rats were divided into two groups randomly. Pancreas ischemic necrosis model was made by deligation,then the purified pancreas MSCs were marked with DAPI and then were transplantated partially. After two weeks,the survival rate was measured and histopathological detection was performed. Results ① The cells had concordant morphous gradually with vigorous generation. There was no significant difference in morphology and surface antigen compared with BM-MSCs.② The survival rate in experimental group was 75% ,the necrotic tissue had basi-rebounded. Blue fluorescent was observed in repaired pancreas tissues. The survival rate in control group was 20%. The survival rate in experimental group was higher than that in control group (P

OBJECTIVE To evaluate the antibacterial activities of cefoperazone combined with sulbactam against Gram negative bacteria,and compare with other antimicrobial agents.METHODS The antibacterial activities of 10 frequently used antibiotics against 1 670 strains of clinical isolated Gram negative bacteria were studied by using agar dilution methods according to the NCCLS 2002.RESULTS The results showed imipenem was the most active tested against Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae,followed by cefoperazone-(sulbactam),which had similar activities as imipenem against the non-fermentative strains such as Pseudononas aeruginosa,and Acinetobacter spp,but cefoperazone-(sulbactam) had higher susceptibility to imipenem-(resistant) Stenotrophomonas maltophilia.CONCLUSIONS Cefoperazone-sulbactam has good and broad spectrum(antibacterial) activities especially against Gram negative(bacteria,) and is expected to have a bright prospect in the treatment of severe hospital infections induced by Gram negative bacteria.

Objective To study the expression of vascular endothelial growth factor(VEGF) in carcinoma of cheek in order to investigate the relation with carcinogenesis and development of carcinoma of cheek.Methods The expression levels of VEGF were determined by S-P method of immunohistochemistry technique in 40 tissues of carcinoma of cheek,30 para-carcinoma and 10 normal mucosa.Results The expression rates of VEGF in the tissues of carcinoma of cheek,para-carcinoma and normal mucosa were 85.0%,26.7%,10.0%,respectively.There was remarkable difference in the statistic(?~2=32.26,P

0.05). Phagocytic index of RPE cells was (28.7?1.9)% in the presence of 10 ?mol?L~ -1 DIDS,10 ?mol?L~ -1 DIDS significantly inhibited the nonspecific phagocytic process of human RPE cells(P

Objective To investigate the features of the cerebrospinal fluid (CSF) in the modified ZeiM-Neelsen (MZN) positive tuberculous mengningitis (TBM).Methods We retrospectively reviewed the clinical data of 170 patients with tuberculous meningitis confirmed by MZN stain from December 2012 to July 2015.The purpose of the present study was to investigate the relationship of MZN staining and CSF cytology.Results Among 170 patients with TBM confirmed by MZN staining,128 cases had first detectable acid-fast bacillus (AFB) in earlier stage.The cytology included 15.5％ mixed cellular cytology,58.5％ lymphoid cytology,19.5％ neutrophilic cytology and 6.5％ normal cytology.Twenty-four cases had first detectable AFB within 1-2 months following disease onset.The cytology included 13.1％ mixed cellular cytology,56.6％ lymphoid cytology,21.7％ neutrophilic cytology and 8.7％ normal cytology.Eighteen cases had first detectable AFB 2 months after disease onset.The cytology included 26.7％ mixed cellular cytology,46.7％ lymphoid cytology,20.0％ neutrophilic cytology,6.6％ normal cytology.There was no significant difference in median time of first detectable AFB among those four types of cytology (P=0.812).There was significant difference in median time of first detectable AFB between patients with and without anti-TB therapy [21.5 (12.3,37.8) days vs.8.5 (6.0,16.3)days,P＜0.001].There was no significant difference in median time MZN stain turning negative between patients with and without anti-TB therapy [11 (5.75,19.25) days vs.6(4.25,10.75)days,P=0.230].Conclusions AFB can be detectable within a month after the onset of TBM in most of cases.(MZN) positive staining is not associated with the major type of cytology.Anti-TB therapy may delay the first detectable time of AFB.