1.Comparison of Work Mode and Effect Analysis in ICU Area of Our Hospital before and after the Applica-tion of Automated Drug Dispensing System
Fang WANG ; Sha LI ; Wang LU ; Yajing SUN ; Lei CHEN
China Pharmacy 2016;27(19):2660-2662
OBJECTIVE:To promote standard management of drugs in ICU area. METHODS:The change of work mode in ICU area of our hospital was analyzed and compared after the application of automated drug dispensing system(ADDS)as well as the change of related indicators 3 months before and after the application of ADDS;the effect of ADDS was evaluated. RESULTS& CONCLUSIONS:After the application of ADDS,the management of base drugs changed from open management to intelligent, closed-off and authority management;drug storage and cost management became more scientific,and staff allocation became more optimal. Under the condition of zero difference in the number of medical orders,initial medication time shortened from 170 min to 131 min (P<0.05),time of daily drug preparation and drug repercussion shortened from (81.20 ± 3.56)min to (30.04 ± 7.55) min,and (9.18 ± 2.79)min to (6.47 ± 4.66) min (P<0.05). The application of ADDS can improve work efficiency,standardize drug management,promote medical safety,and provide reference for initiating a new mode of drug management in wards.
2.The expression and clinical significance of the serum Eotaxin, IL-13 and total IgE in children with asthma
Chunyan MA ; Yajing ZHANG ; Lihua NING ; Hua CHEN ; Fang ZHOU ; Xin WANG
International Journal of Pediatrics 2011;38(2):195-197
Objective To study the nosogenesis and the relationship of different levels of serum Eotaxin,IL-13 and total IgE(TIgE)in asthmatic children. Methods Thirty children with asthma aging from 5 to 12 years were selected as study group (attack period and remission period), and 22 healthy children were selected as control group. Serum Eotaxin and IL-13 were measured with enzyme linked immunosorbent assay, and serum TIgE was determined with enzyme-linked fluoroimmune assay in the study group and control group, and pulmonary function was measured at the same time in the study group. Results (1) There were significant differences in pulmonary function between the attack period and the remission period (P < 0. 001). (2) There were significant differences in the level of serum Eotaxin, IL-13 and IgE between the study group and the control group,especially for the attack period and the control group(P <0. 001). (3) There were significant positive correlations between the level of serum Eotaxin and IL-13, Eotaxin and TIgE, IL-13 and TIgE respectively in the study group (P <0. 05). (4) There were negative correlcations between the level of serum Eotaxin and FEV1 ,PEF,and serum IL-13 and FEV1, PEF in asthmatic children (P < 0. 05), there were no correlations between the level of serum TIgE and FEV1, PEF in the study group (P > 0. 05). Conclusion Serum Eotaxin, IL-13 and TIgE were participated in the nosogenesis of asthma, and the level of serum Eotaxin and IL-13 can be a assessment of asthmatic patients' condition.
3.The expression and clinical significance of the serum IL-17/LTB4/IgE in children with asthma
Chunyan MA ; Xin WANG ; Lihua NING ; Yajing ZHANG ; Hua CHEN ; Fang ZHOU ; Jinli HAO ; Rui ZHOU
International Journal of Pediatrics 2012;39(4):428-431
Objective To explore the impact of levels of serum IL-17,Leukotriene B4 and IgE on pathogenesis of childhood asthma.Methods Totally 60 children with asthma acute exacerbation ( 29 children with mild asthma,31 children with moderate-severe asthma) were selected as study group,24 healthy children were selected as control group.Serum IL-17 and LTB4 were measured with euryzemLinked immunosorbent assay,serum IgE was determined with enzyme-linked fluoroimmuneassay by pharmacia CAP Sytem,PMN was determined with automatic blood analyser,pulmonary function was measured in the study group.Results ( 1 ) The level of serum IL-17 ( 1.15 ± 0.10 μg/L,2.80 ± 2.30 μg/L,0.83 ± 0.10 μg/L),LTB4 (2.22 ± 1.01 μg/L,8.79 ± 9.36 μg/L,1.94 ± 1.13 μg/L) and IgE( 123.70 ±86.94 μg/L,322.27 ±332.28 μg/L,24.27 ±7.64 μg/L) were significantly different among mild asthma group,moderate-severe asthma group and control group( P < 0.001 ).( 2 )The N% of mild asthma group,moderate-severe asthma group and study group were( 55.06 ± 1 1.15 ) %,( 64.44± 11.87)%,(47.96 ± 13.52)%,L% were(42.20 ± 11.04)%,(33.93 ± 10.02)%,(49.65 ± 13.02)%,and there were significant differences in N% and L% between study group and control group( P < 0.05 ).( 3 ) There were significant positive correlations between the serum IL-17 levels and IgE,LTB4 and IgE,IL-17 and LTB4 in asthmatic children( P <0.05).(4) There were significant negative correlcations between the level of serum IL-17,LTB4 and FEVI,PEF( P <0.001 ).There were significant positive correlations between serum IL-17,LTB4 and N% (P <0.001 ).(5)There were not correlations between the level of serum IgE and FEV1,PEF and N%in asthmatic children( P >0.05 ).Conclusion The levels of serum IL-17,LTB4 and IgE participated in pathogenesis on asthmatic children patients.
4.Glutamine regulates the proliferation and survival of small cell lung cancer H446 cells
Pengyu XU ; Jiayin LI ; Yajing MIAO ; Cuicui GAO ; Yao SHEN ; Fang JIN ; Xiaofei QIU
Tianjin Medical Journal 2016;44(12):1423-1427
Objective To investigate the effects of glutamine (Gln) on proliferation and survival of small cell lung cancer H446 cells, and further to explore the potential mechanism. Methods The proliferation of H446 cells was detected at different time points (0, 24, 48, 72 and 96 h) by CCK-8 assay in Gln (+) group and Gln (-) group, and an optimal time was selected. Under the optimal time, Annexin V-FITC/PI staining, CellTiter-Glo? assay kit and flow cytometer were used to detect cell survival, cellular adenosine triphosphate (ATP) and reactive oxygen species (ROS) levels. Gln (-) group was used as the control group, under the condition of Gln deficiency, cellular ATP, cell proliferation and survival were detected after adding oxaloacetic acid (OAA) or dimethyl-α-ketoglutarate (DM-αKG). Gln (-) group was used as the control group, cellular ROS, cell proliferation, colony and survival were detected after treated with ROS scavenger N- acetyl cysteine (NAC). With different concentrations (0, 2, 5, 10 μmol/L) of glutaminase inhibitor BPTES, the optimal concentration was selected through the colony assay. The cellular ATP and ROS levels and cell proliferation were detected under the optimal concentration. H446 cells were treated with bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl) ethyl sulfide (BPTES), ROS inducer hydrogen peroxide (H2O2) or the combination of them, and cell survival ratio was compared between two groups. Results The proliferation levels of H446 cells at 24, 48, which were decreased most significantly in 72 h in Gln (-) group. When 72 h was used as the optimal time, the cell survival ratio and ATP level were decreased, and the ROS level was increased, in Gln (-) group compared with those of Gln (+) group (P<0.05). There was a higher survival ratio in H446 cells in Gln (-)+OAA group and Gln (-)+DM-αKG group than that of Gln (-) group (P<0.05), but there were no significant differences in cell proliferation and ATP levels between Gln (-) group, Gln (-)+OAA group and Gln (-)+DM-αKG group. The ROS level was reduced, the cell proliferation, colony level and survival ratio were increased in Gln (-)+NAC group compared with those of Gln (-) group (P<0.05). Cloning assay showed that 10μmol/L was the optional concentration. Under this concentration, the proliferation and ATP level were decreased in Gln(+)+BPTES group (P<0.05), and cellular ROS level was up-regulated compared with Gln(+) group. The survival ratio was significantly lower in BPTES+H 2O2 group compared with BPTES (+) group or H2O2 (+) group. Conclusion Glutamine deficiency inhibits the proliferation and survival ratio of H446 cells through enhancing ROS level. BPTES and H2O2 show synergistically inhibitory effect on the survival of H446 cells.
5.Clinical study of ulinastatin combined with CRRT in the treatment of multiple organ dysfunction syndrome
Hongshan KANG ; Yan BAI ; Yajing LIU ; Jing WANG ; Shuhong LIU ; Huiqing WANG ; Zhen MA ; Fang DING ; Zhaobo CUI
Chongqing Medicine 2017;46(11):1478-1481
Objective To evaluate the clinical curative effect of ulinastatin combined with CRRT in the treatment of multiple organ dysfunction syndrome(MODS).Methods Sixty eight patients with MODS who were admitted to ICU from July 2013 to July 2015 were randomly divided into three groups:control group,CRRT group,combined group;Patients' APACHE Ⅱ,SOFA scores level of inflammatory markers were recorded before treatment and after treatment of 72 hours and 7 days.The mortality of the three groups in ICU were compared.Results After 72 hours and a week of treatment,the level of IL-10,IL-6,TNF-α,WBC、PCT、CRP in CRRT group and combined group were significantly better than that of control group(P<0.05),and combined group were significantly better than that of CRRT group.Compared with the control group,the oxygen index,lactic acid,ALT significantly im proved in CRRT group and combined group were better than control group,after 72 hours and a week of treatment(P<0.05),and the cornbined group was the most obvious.After a week of treatment,the mortality rate of CRRT group and combined group was significantly better than the control group (P<0.05),while there was no statistical differences between CRRT group and combined group(P>0.05).Conclusion Ulinastatin combined with CRRT is an effective method for the treatment of MODS.
6.Advances in the research on mechanism of tumor metabolism regulated by c-Myc
Yahui LIU ; Lu GAO ; Yajing WANG ; Fang YAN
Journal of China Pharmaceutical University 2021;52(3):379-386
The transcription factor c-Myc regulates the proliferation, differentiation, metabolism and other key processes of normal cells extensively.The unleashed MYC oncogene frequently produces abundant c-Myc protein, which directly regulates the gene expression of key metabolic enzymes, or tumor-related metabolic pathways by inhibiting microRNA, leading to abnormal metabolism characterized by heightened nutrients uptake, enhanced glycolysis and glutaminolysis, and elevated fatty acid and nucleotide synthesis.This paper briefly summarizes how c-Myc regulated metabolism on glycolysis, glutamine metabolism, tricarboxylic acid cycle, lipid metabolism and nucleotide synthesis in cancer cell,which provides some theoretical reference for the development of antitumor targets and drugs involving c-Myc.
7.Research progress on the effects of glucagon-like peptide-1 receptor agonists in stroke
Yajing WANG ; Penghua FANG ; Zhenwen ZHANG
Chinese Journal of Nervous and Mental Diseases 2023;49(9):549-553
Stroke is a prevalent acute cerebrovascular disease associated with significant morbidity and mortality,which highly demand effective prevention and treatment strategies.Glucagon-like peptide-1 receptor agonists(GLP-1RA)is a novel type of antidiabetic drug that exerts hypoglycemic and weight loss effects on GLP-1 receptors.Additionally,GLP-1RA possess the potential to reduce infarction size and promote nerve recovery by inhibiting inflammation,oxidative stress,apoptosis,and improving blood-brain barrier permeability and other pathologies.This paper provides a comprehensive summary of the role of GLP-1 in stroke occurrence while also explores the underlying mechanisms by which GLP-1RA influences stroke pathogenesis.Furthermore,it briefly reviews the therapeutic efficacy of GLP-RA in managing stroke.
8.Effect of salinomycin on proliferation and autophagic flow of human malignant melanoma M21 cells
Yajing LIU ; Guoyu LIU ; Shubin WANG ; Zhengyu FANG
Practical Oncology Journal 2018;32(3):203-207
Objective The objective of this study was to investigate the effect of salinomycin on proliferation and autophagic flux of human melanoma M21 cells. Methods The cell survival rate was determined by MTS assay and IC50values( half inhibitory concentration)were calculated. The morphological changes of cells after salinomycin administration were observed under optical micro-scope. Flow cytometry was used to examine the apoptosis rate of M21 cells. Western blot was used to detect the expression of autophag-ic-related protein LC3B and p62 in M21 cells. The presence of autophagosomes in M21 cells after salinomycin administration was ob-served under transmission electron microscope. Western blot and immunofluorescence were used to detect the level of p62 protein and localizing changes in M21 cells. Results Salinomycin significantly inhibited proliferation of M21 cells, and the IC50values were (1. 38 ± 0. 18)μM. After salinomycin administration,the proliferation rate of M21 cells was slowed down,and obvious vacuoles ap-peared in the cells. Salinomycin could not only induce cell apoptosis,but it also increased the ratio of LC3B-Ⅱ/LC3B-Ⅰ in M21 cells. The increase and accumulation of autophagosomes were directly observed under transmission electron microscope. The level of p62 protein was slightly elevated after salinomycin treatment and gradually aggregated into the cytoplasm,indicating that autophagic flux was inhibited. Conclusion Salinomycin can inhibit the proliferation of human malignant melanoma M21 cells,and its mechanism may be related to the accumulation autophagosomes granules and inhibition of autophagic flux.
9.Visual analysis of critical care research based on Web of Science core set
Wenyan XI ; Xiaoxu ZHANG ; Pengbo YAN ; Kaili SHU ; Yajing QIN ; Aihong FANG ; Yongsheng LI
Chinese Journal of Practical Nursing 2022;38(14):1115-1121
Objective:To analyze the data of critical care related literatures published in Web of Science core set by knowledge map visualizing technology, and summarize the research status and hotspots of critical care.Methods:The core set database of Web of Science from January 2011 to December 2020 was searched, the extracted information was standardized and analyzed by knowledge map visualizing technology.Results:A total of 783 articles in the field of critical care were included, and the number of literatures increased year by year, among which the number of articles published in the American Journal of Critical Care(305) was the most. The United States (437) was the country with the most published literatures, and Australia was the country with the highest centrality (centrality=0.67). The number and centrality of articles published from China were at the lower middle level. There was cooperation among countries, but not close cooperation. The cooperation among authors was in the state of small aggregation and large dispersion. Cluster analysis and keyword emergence analysis showed that many aspects in the field of intensive care had been the research hotspots, among which "post intensive care syndrome" and "children′s intensive care" were the research hotspots in recent years.Conclusions:The research field of intensive care is still in the rising stage of development, and post intensive care syndrome and children's intensive care are the latest hotspots in intensive care research.
10.Burden of disease of colorectal cancer in the Chinese population, in 1990 and 2013
Yajing FENG ; Ning WANG ; Liwen FANG ; Shu CONG ; Peng YIN ; Yichong LI ; Maigeng ZHOU
Chinese Journal of Epidemiology 2016;37(6):768-772
Objective To analyze the disease burden of colorectal cancer in the Chinese people in 1990 and 2013.Methods Data from the estimation of 2013 Chinese Sub-national Burden of Disease Study was used,to analyze the incidence and mortality of colorectal cancer in China,in 1990 and 2013,among all the Chinese people.Data on the years of lifte lost due to premature mortality (YLL),years lived with disability (YLD) and disability-adjusted life years (DALY) of colorectal cancer in China,in 1990 and 2013 was also used.Related death rates,DALY and DALY rates were compared.Results The standardized incidence rate of colorectal cancer among Chinese people increased by 66.83% from 12.15/100 000 in 1990 to 20.27/100 000 in 2013 (72.82% for men and 55.26% for women).The standardized mortality rate of colorectal cancer increased by 4.73% from 10.57/100 000 in 1990 to 11.07/100 000 in 2013 (with 9.03% increase for men but 3.12% decrease for women).A total number of 148 000 people died from colorectal cancer in 2013,with an increase of 90.08% (105.36% for men and 72.14% for women) compared with the number of 77 900,in 1990.Related data on DALY,YLL and YLD due to colorectal cancer appeared as 3 331.6 thousand person years,3 200.7 thousand person years and 130.9 thousand person years,respectively in 2013,with YLL as the vast majority of DALY.Compared with 2 019.1 thousand person years in 1990,DALY showed an increase of 65.01% in 2013.However,the standardized DALY rate reduced by 6.00% from 238.54/100 000 in 1990 to 224.23/100 000 in 2013.Parameters as the mortality rotes,DALY and DALY rates appeared all higher in men than in women,in every age group.Conclusions The disease burden of colorectal cancer in China remained at high levels in 2013.Compared with the data in 1990,the total disease burden on colorectal cancer showed an increase while the DALY rate and YLL rate showed a decline in 2013.