Objective:To evaluate the protective effect of ?-opioid peptide,[D-Ala~(2)-D-Leu~(5)]-enkephaline(DADLE), on pulmonary ischemia-reperfusion(I-R) injury after cardiopulmonary bypass(CPB),and to discuss the possible mechanism.Methods: Twenty-eight male pigs were evenly randomized divided into 4 groups,namely,the sham-operation group,the CPB group(only subjected to CPB),the DADLE pretreatment group(received DADLE 1 mg?kg~(-1)before CPB),and the Glibenclamide group(received DADLE 1 mg?kg~(-1)and glibenclamide 1 mg?kg~(-1) before CPB).Peak inspiratory pressure(PIP),PaO_(2),and the plasma malondialdehyde(MDA) concentration were measured in each group before CPB and 10 min,30 min,and 60 min after reperfusion.The changes of lung tissue wet weight/dry weight ratio(W/D) were calculated before CPB and 60 min after reperfusion.The animals were sacrificed 60 min after reperfusion to observe the ultrastructural changes of the lungs and to quantitatively assess lung tissue damage(LTD).Results: There were no significant differences among the 4 groups before CPB and the parameters in(sham-operation) group had no changes at all specified time points.One hour after reperfusion,the PIP,MDA,W/D,and LTD values in Glibenclamide and CPB groups were significantly higher than those in sham-operation and DADLE groups while the PaO_(2) was significantly lower(P

Acute traumatic coagulopathy (ATC) attacks suddenly with a rapid progress and has an extremely highly mortality.Of late years more and more relative studies have made a great progress on the pathogenesis,diagnosis and treatment of ATC.However,there are currently no medical guidelines for ATC.There are still some problems such as nonstandard treatment,outdated concept and so on in clinic.Based on the latest research progresses home and abroad,this paper expounds emphatically the first-aid and treatment of ATC in order to bring helps to the clinical doctors.

Objective To discuss the main reason of re -pregnant patients'delivery mode selection after cesarean delivery and to guide the future clinical work.Methods Retrospectively analyzed the 181 cases of re -pregnancy after cesarean delivery and compare the clinical data of 140 cases of reselecting cesarean delivery with 41 cases of selecting vaginal trial production.Results Calculated the risks of vaginal trial production and cesarean delivery of scarred uterus by Smith formula.There was no statistically significant difference in two groups (P >0.05). Reselection of cesarean delivery accounted for 77.0% among the cases of re -pregnancy after cesarean delivery.In the investigation on the reason for re -pregnant patients reselecting cesarean delivery,38 patients chose cesarean delivery for the first time owing to social factors,accounted for 27.1%,taking the first place.While in the investiga-tion on the reason for re -pregnant patients selecting vaginal trial production,10 patients were operated on cesarean section for the first time due to fetal distress,accounted for 24.4%,coming first.Conclusion In the investigation on the reason for re -pregnant patients reselecting cesarean delivery,the result shows that the high possibility of reselect-ing cesarean delivery lies on those who chose cesarean delivery for the first time owing to social factors.While those who were transferred to cesarean section due to abnormal fetal heart rate during the first vaginal trial production,may have high possibility to select vaginal trial production when re -pregnancy.As a result,the key is to decrease the rate of cesarean delivery for the first time.

Adjuvants have been used as critical components of vaccine.They were used to stabilize the antigens and potentiate the immune responses.Dose sparing is also of interests in recent years to be applied in potential pandemic situations or to lower the costs of vaccines.With the emerging nanotechnology in many aspects including the use in biomedical applications,the application of nanomaterials as vaccine adjuvants also attracted a lot of attentions in recent years.With favorable biological properties,such as well-defined and well-formed nanoparticles,biocompatibility,biodegradability and ability to induce humoral immunity and cellular immunity simultaneously,calcium phosphate nanoparticles (CaP) have the potentials to be developed as an immune potentiator to be used as vaccine adjuvants.Here,we reviewed the basic properties of CaP and their applications as vaccine adjuvants with antigens of different modalities such as inactivated virus,protein and naked DNAs.The mechanism of the adjuvanting effects is briefly described.Further the development of CaP as vaccine adjuvants with some designed surface modification could lead to next generation vaccine adjuvants with improved immune potentiating properties and safety profiles.

OBJECTIVE: To discuss the impact of Zero Inventory of Chinese decoction pieces in our hospital. METHODS: Zero Inventory of Chinese decoction pieces was analyzed from aspects of hospital resource utilization, pharmacy management, and quality assurance of decoction pieces. RESULTS: Zero Inventory can help save precious hospital resources, streamline pharmacy management and ensure quality of decoction pieces supply. CONCLUSION: Zero Inventory of Chinese decoction pieces has positive impact on hospital pharmacy management; therefore it deserves to be popularized.

To study the effects of Xuesaitong Injection, an extract from a Chinese herbal medicine, on coagulation function of gastric cancer patients undergoing laparoscopic surgery.

Objective: To study the effect of taurine to resist the impact of lead on learning and memory. Methods: ABC immunohistochemistry method was used to study the quantity change of nNOS positive neuron in hippocampus of the rats which were fed with distinct dosage of lead acetate in drinking water (0.02, 0.2 g/L) and distinct dosage of taurine (5,10 g/kg) in feed. Rusults: Taurine could increase nNOS positive neuron quantity obviously in hippocampus of rats induced by lead lesion. Conclusion: Taurine could resist impact of lead on learning and memory obviously.

Aim To study the effect of silymarin on proliferation and apoptosis of human osteosarcoma saos-2 cells and to explore its possible mechanisms .Meth-ods Control group and different concentration groups of silymarin were set , and Saos-2 cells were treated with silymarin .Celluar morphologic changes were ob-served by inverted phase contrast microscope .Prolifer-ation of cells was tested CCK-8 assay .Apoptosis rate of cells was analyzed by flow cytometry . ERK1/2、p-ERK1/2 and cleaved caspase-3 protein expression were measured by Western blot .Results Silymarin inhibi-ted the proliferation of Saos-2 cells in a time-dependent and concentration-dependent manner . Silymarin in-duced the apoptosis of Saos-2 cells in a concentration-dependent manner . Silymarin decreased p-ERK1/2 protein expression and increased cleaved caspase-3 protein expression in a concentration-dependent man-ner, while ERK1/2 protein expression had no obvious change.Conculsions Silymarin can inhibit the prolif-eration of human osteosarcoma saos-2 cells and induce apoptosis of them , and the mechanism may be related to inhibition of ERK signaling pathway and upregulated caspase-3 protein expression .

Objective To compare the effect of water-infusion colonoscopy and conventional air-infusion colonoscopy on the bowel cleanliness during withdrawing.Methods A single blind randomized controlled trial was conducted. 222 patients were randomly divided into water-infusion group (group A, 112 cases) and air-infusion group (group B, 110 cases). The cleanliness scores, scores improvement, pain scores, time of reaching cecum, depth of colonoscopy, rate of whole colon examination, adenoma detection rate (ADR), operator’s dififculty and complications were compared between the two groups.Results The cleanliness score during withdrawing was higher in group A [M(8)/IQR(1) vs M(8)/IQR(2),P = 0.000], the improvement was higher in group A [(0.53 ± 0.74) vs (0.23 ± 0.55), P = 0.000], the abdominal pain score was signiifcantly lower in group A [M(2)/IQR(1) vs M(4)/IQR(2),P = 0.000] and the ADR was higher in group A (36.61 % vs 23.64 %,P = 0.041). The operator’s dififculty evaluation score was signiifcantly lower in group A [M(1)/IQR(1) vs M(2)/IQR(1),P = 0.005]. There were no signiifcant differences at the time of reaching cecum, the depth of colonoscopy and the whole colon examination rate between the two groups.Conclusion Water-infusion colonoscopy could signiifcantly improve the bowel cleanliness during withdrawing scope, improve the ADR and reduce abdominal pain of patients, without increasing the time to reach cecum.

Objective: To compare the effects of nicardipine and labetalol on hypertension during laparoscopic cholecystectomy.Methods:Thirty patients with hypertension undergoing selective laparoscopic cholecystectomy were randomly divided into 3 groups. The control group was only given anesthetic drugs; nicardipine group was given nicardipine 20 ?g?kg -1 ?min -1 before induction, followed by intravenous continuous infusion of nicardipine at the rate of 0.5 1.5 ?g?kg -1 ?min -1 ; labetalol group received labetalol 0.3 mg?kg -1 ?min -1 before induction followed by intravenous continuous infusion of labetalol at the rate of 10 20 ?g?kg -1 ?min -1 . Results: Statistical differences( P