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Database of analgesic traditional Chinese prescriptions manages 3 sub-databases of information on disease, herbs, and compound prescription intelligently and relatively. When a key word is input into the database, the associated information of herbs, their compound prescriptions, actions and effects will be displayed immediately. Starting from setting inclusion criteria, the module of database was designed according to application purpose, and the searching strategy was designed according to regularity of prescription compatibility. The compound prescription can be quantitatively estimated as monarch, minister, assistant and guide in order.

In this study, we developed a novel liposome-silica hybrid nano-carrier for tumor combination therapy via oral route, using paclitaxel and cyclosporine as a model drug pair. Optimization of the preparation of the drug-loading formulation and characterization of its physicochemical parameters and drug release profile were performed in vitro. Then in vivo pharmacodynamics and pharmacokinetics studies were performed. The results showed that the obtained formulation has a small particle size (mean diameter of 100.2 +/- 15.2 nm), a homogeneous distribution [the polydispersity index was (0.251 +/- 0.018)] and high encapsulation efficiency (90.15 +/- 2.47) % and (80.64 +/- 3.52) % for paclitaxel and cyclosporine respectively with a mild and easy preparation process. A sequential drug release trend of cyclosporine prior to palictaxel was observed. The liposome-silica hybrid nano-carrier showed good biocompatibility in vivo and co-delivery of cyclosporine and paclitaxel significantly enhanced the oral absorption of paclitaxel with improved anti-tumor efficacy, suggesting a promising approach for multi-drug therapy against tumor and other serious diseases via oral route.

OBJECTIVE To discuss the risk factors of professional exposure of dental health personnel and treatment measures. METHODS The management system of standardization,systematization,science and universal prevention were carried out and realized to maximally prevent the onset of dental hospital infection. RESULTS A set of feasible management system and measures about professional exposure for dental health personnel were drafted to make dental staff master the management standards of dental infection,realize the sterilization procedures of dental device and prevent dental infection maximally in dental hospital by implementing universal preventive measures during dental treatment. CONCLUSIONS The preventive measures and programs about professional exposure for dental health personnel must be structuralized.

Aim To establish ICR animal model with C6 glioma stem cells, to provide the ideal model for the further study of gli-oma stem cells in brain glioma model. Methods C6 glioma stem cell was cultured in vitro by suspension,and was identified with Nestin antibody. C6 stem cells of ICR mouse glioma model were used to investigate survival state and tumor volume in mice after the operation. HE staining and CD133 immunohistochemi-cal study were adopted to investigate the postoperative pathologi-cal changes in mice. Results The expression of Nestin was 96. 01% in C6 glioma stem cells, and Nestin was highly ex-pressed in the cultured C6 glioma stem cells. Mice were inocula-ted with tumor after loss of appetite, weight, behavior and slow, sluggish reaction. Tumor volume at day 21 after modeling was (9. 77 ± 6. 58) mm3 . After HE staining, the model showed the invasive growth, tumor cell shrinkage and derangement. Immu-nohistochemical CD133 staining revealed that tumor cytoplasm color was brown. Conclusion Glioma model can be established based on glioma stem cells into a high rate of tumor, the tumor cycle is short, which can be used as an ideal model for glioma.

Objective: To study the effect of taurine to resist the impact of lead on learning and memory. Methods: ABC immunohistochemistry method was used to study the quantity change of nNOS positive neuron in hippocampus of the rats which were fed with distinct dosage of lead acetate in drinking water (0.02, 0.2 g/L) and distinct dosage of taurine (5,10 g/kg) in feed. Rusults: Taurine could increase nNOS positive neuron quantity obviously in hippocampus of rats induced by lead lesion. Conclusion: Taurine could resist impact of lead on learning and memory obviously.

The ancient books on traditional Chinese medicine are mainly covered in the comprehensive bibliogra-phies and special bibliographies of traditional Chinese medicine in the past ages.Their classification was thus de-scribed in the Seven-Classification System, Four-Classification System, Twelve-Classification System,Bibliogra-phy of medical collection and General Bibliography of ancient books on traditional Chinese medicine, followed by an analysis of the classification systems of ancient books on traditional Chinese medicine in the past ages and their evo-lution, which helps to search the medical literature from the vast ancient books according to their classification.

The effects of melittin on the activities of Na+-K+-ATPase and glucose-6-phosphate dehydrogenase (G-6-PD) which are on the membrane of red blood cell (RBC) are chosen as the index of this study. The possible target sites of these effects through enzyme activity determination by spectrophotometry are investigated, and the hemolytic process and the activity change of these two types of enzymes on the RBC membrane are discussed. The results show that the main mode of melittin inhibition to the activity of enzymes on the RBC membrane is the coexistence of adhesion/insertion form and free-state form, and the effect of the former is more stronger than the latter. The K+ binding site of Na+-K+-ATPase is one of the target sites of melittin. The membrane-insertion process of melittin synchronizes with the action of melittin on this enzyme. Melittin slowly inhibits the catalysis of G-6-PD through the action on G-6-P and NADP, and the extent in which melittin forms tetramers isclosely related to the enzyme activity. EDTA inhibits the aggregation of melittin, and interferes with its action on G-6-P. The biochemical mechanisms of melittin effects on the substrate G-6-P and the coenzyme NADP are similar, and the inhibition of melittin is not related to the structure of G-6-PD.

OBJECTIVE To analyze the etiological distribution and drug resistance characteristics of patients with respiratory failure and lung infection in EICU.METHODS The types of respiratory failure,the incidence condition of ventilator-associated pneumonia(VAP),the mortality rate,and the drug sensitivity of 129 pathogen strains which were isolated and cultured from the 110 cases with respiratory failure and pneumonia between Jan 2006 to Aug 2008 in EICU were analyzed.RESULTS Among the 110 patients,48 cases were died(the mortality rate of 43.6%).There were 28 cases(58.3%) of Ⅱ type respiratory failure,20 cases(41.7%) of Ⅰ type respiratory failure.Of 129 pathogens strains,there were 29 Gram-positive bacteria(22.5%) and 100 Gram-negative bacteria(77.5%).The major Gram-negative bacteria were Pseudomonas aeruginosa,Acinetobacter baumannii,and Klebsiella pneumoniae,and they showed generally higher multidrug resistance.Except minocycline,A.baumannii appeared strong multidrug resistance to the other 16 kinds of common antibiotics.The main Gram-positive bacteria were Staphyococcus haemolyticus,S.aureus and coagulase-negative staphylococci(CNS),and no evidence showed they resisted to vancomycin.CONCLUSIONS There are high mortality rate of patients with respiratory failure and lung infection if they suffered from compound infection.The main pathogens of the lung infection are Gram-negative bactiera,such as P.aeruginosa,A.baumanniiand K.pneumoniae in order,with high multidrug resistance.The main Gram-positive bacteria show no evidence resist to vancomycin.

In order to investigate the inhibitory effect of plasmid-mediated short hairpin RNA targeting vascular endothelial growth factor (VEGF) on the expression of VEGF mRNA in human gastric cancer cells, a plasmid vector for transcribing specific short hairpin RNA targeting VEGF (pU6-VEGF) was constructed, and then transfected into human gastric cancer cells using Lipofectamine2000. The VEGF mRNA expression level was detected by RT-PCR. RPMI1640 was used for blank control, and pSilencer 1.0-U6 empty plasmid for the negative control. Results showed the clone and sequence analysis revealed that the recombinant plasmid vector of pU6-VEGF was successfully constructed. The VEGF mRNA expression levels in blank control group, experimental group (pU6-VEGF) and negative control group (pSilencer1.0-U6) were 100%, 49% and 94%, respectively, indicating VEGF mRNA expression in the cells transfected with pU-VEGF vector was inhibited significantly as compared with blank control group and negative control group. It was concluded that the short hairpin RNA could significantly inhibit the expression of VEGF mRNA, which provided an experimental basis for treating human cancer with anti-angiogenesis.