Objective To investigate the effect of bedside comprehensive ability training and critical thinking on the improvement of the personality maturity of pediatric nurses. Methods A total of 102 nurses with pediatric working years within one year were selected,and then they were arranged to receive critical thinking in combination with bedside comprehensive ability training for 6 months. After the training, pediatric nurses′ critical thinking ability, bedside comprehensive ability and personality maturity were evaluated. Results Before training, the total score of Critical Thinking Disposition Inventory-Chinese Version (CTDI-CV) , total score of bedside comprehensive ability, personality maturity score were 288.21±25.44, 75.24±2.51, 84.01±32.26, after training, they were respectively 324.18±25.93, 92.65±3.17, 125.57±35.47. Comparison of the scores before training and after training showed statistically significant difference(t=7.071, 30.750, 6.190,P < 0.05 or 0.01). Setting personality maturity as the dependent variable, and and CTDI-CV and bedside comprehensive ability as the independent variables, regression analysis showed that CTDI-CV and bedside comprehensive ability had a positive impact on the personality maturity. Conclusions The ability of pediatric nurses' critical thinking ability and bedside comprehensive ability training can promote the improvement of personality maturity of pediatric nurses.

Objective To explore the potential mechanism of recombinant ficolin 3(FCN3)in regulating non-small cell lung cancer cell line A549.Methods Bioinformatics analysis of FCN3 was performed using online software,and its overexpression vector and siRNA interference system were constructed.CCK8 assay and flow cytometry were used to detect the effects of overexpression and knockdown of FCN3 on the proliferation and apoptosis of A549 cells.Real-time fluorescent quantitative polymerase chain reaction was used to detect the influence of FCN3 on the expression of lung adenocar-cinoma-related genes[Chromobox Homolog 5(CBX3),Cyclin-Dependent Kinase Inhibitor 3(CDKN3)and Baculoviral IAP Repeat-Containing Protein 5(BIRC5)].Results After overexpres-sion of FCN3,the apoptosis level of A549 cells increased,and the proliferation level decreased(P＜0.05).After knockdown of FCN3,the apoptosis level of A549 cells decreased,and the proliferation level increased(P＜0.05).Overexpression of FCN3 inhibited the expression of CBX3,CDKN3 as well as BIRC5 mRNA,and promoted the expression of FABP4 mRNA;knockdown of FCN3 promoted the expression of CBX3,CDKN3 as well as BIRC5 mRNA,and inhibited the expression of FABP4 mRNA.Conclusion FCN3 can inhibit the proliferation of A549 cells and promote their apoptosis.FCN3 may serve as a potential therapeutic target for non-small cell lung cancer.

Objective To explore the potential mechanism of recombinant ficolin 3(FCN3)in regulating non-small cell lung cancer cell line A549.Methods Bioinformatics analysis of FCN3 was performed using online software,and its overexpression vector and siRNA interference system were constructed.CCK8 assay and flow cytometry were used to detect the effects of overexpression and knockdown of FCN3 on the proliferation and apoptosis of A549 cells.Real-time fluorescent quantitative polymerase chain reaction was used to detect the influence of FCN3 on the expression of lung adenocar-cinoma-related genes[Chromobox Homolog 5(CBX3),Cyclin-Dependent Kinase Inhibitor 3(CDKN3)and Baculoviral IAP Repeat-Containing Protein 5(BIRC5)].Results After overexpres-sion of FCN3,the apoptosis level of A549 cells increased,and the proliferation level decreased(P＜0.05).After knockdown of FCN3,the apoptosis level of A549 cells decreased,and the proliferation level increased(P＜0.05).Overexpression of FCN3 inhibited the expression of CBX3,CDKN3 as well as BIRC5 mRNA,and promoted the expression of FABP4 mRNA;knockdown of FCN3 promoted the expression of CBX3,CDKN3 as well as BIRC5 mRNA,and inhibited the expression of FABP4 mRNA.Conclusion FCN3 can inhibit the proliferation of A549 cells and promote their apoptosis.FCN3 may serve as a potential therapeutic target for non-small cell lung cancer.

Objective:To assess a value of dual energy index(DEI)and effective atomic number(Zeff)of dual-energy computed tomography(DECT)combined with slope of energy spectrum curve in identifying the component of urinary tract stones.Methods:The clinical and DECT imaging data of 111 patients with urinary tract stones who admitted to Nantong Haimen People's Hospital from October 2019 to October 2022 were selected,and the diameters of the urinary tract stones of all patients were<5 mm.The components of outside body stones of all patients were analyzed by infrared spectrum.The accuracy,sensitivity and specificity of the DEI,Zeff,slope of energy spectrum curve and the combination of the them were respectively calculated in identifying the components of urinary tract stones.Results:In 111 patients,the patients with calcium oxalate stones were 75(67.57%),and ones with hydroxyapatite were 15 cases(13.51%),and ones with uric acid stones were 21 cases(18.92%),and there were no other type of stone included cysteine.The differences of CT values(F=487.691,P<0.001),DEI values(F=395.553,P<0.001),Zeff values(F=818.689,P<0.001)and the slopes of energy spectrum curves(H=19.615,P<0.001)were statistically significant among the three types of stones.The overall accuracies of DEI,Zeff and slope of energy spectrum curve were 87.39%(97/111),84.68%%(94/111)and 81.98%(91/111)in identifying the components of urinary tract stone.The range of diagnostic accuracies of DEI,Zeff and the slope of energy spectrum curve was between 80%and 100%for calcium oxalate stones,hydroxyapatite and uric acid stones.The overall accuracy of DEI,Zeff combined with slope of energy spectrum curve was 94.59%(105/111)in identifying the components of urinary tract stones.The diagnostic accuracies of DEI,Zeff combined with slope of energy spectrum curve were respectively 94.59%,94.59 and 100%for calcium oxalate stones,hydroxyapatite and uric acid stones.Conclusion:Compared with the single indicator of DECT,the DEI and Zeff combined with the slope of the energy spectrum curve showed better diagnostic accuracy in identifying the components urinary track stones.

Objective To investigate the correlation of serum osteoprotegerin(OPG)and calci-um levels with cerebral microbleeds in patients with acute ischemic stroke.Methods A total of 97 patients with acute ischemic stroke were selected as the study subjects and divided into cerebral mi-crobleed(group 31 patients with)and non-cerebral microbleed(group 66 patients)based on the re-sults of susceptibility-weighted imaging.Demographic data and laboratory examination indicators were collected from the two groups,and serum OPG and calcium levels were measured.The levels of serum OPG and calcium were compared between patients with different degrees of lesion and bleeding sites.Spearman rank correlation analysis was used to determine the correlations of serum OPG and calcium with cerebral microbleeds.Multivariate Logistic regression analysis was conducted to explore the influ-encing factors of cerebral microbleeds.Receiver operating characteristic(ROC)curves were plotted to assess the predictive value of serum OPG and calcium for cerebral microbleeds.Results Significant differences were observed in age,proportions of patients with drinking and hypertension as well as diabetes,systolic blood pressure,diastolic blood pressure,and serum OPG and calcium levels be-tween the cerebral microbleed group and the non-cerebral microbleed group(P＜0.05).Multivari-ate Logistic regression analysis revealed that older age,history of alcohol consumption,history of hy-pertension,high systolic blood pressure,and high OPG level were independent risk factors for cere-bral microbleeds(OR=1.480,1.330,1.843,1.632,1.652;P＜0.05),while high calcium level was an independent protective factor for cerebral microbleeds(OR=0.721,P＜0.05).The AUC values for the prediction of cerebral microbleeds in acute ischemic stroke patients using serum OPG or calcium alone,and their combination were 0.853,0.825,and 0.921,respectively,with the combined prediction showing higher value than the individual prediction(Z=2.895,3.138;P＜0.05).Spearman rank correlation analysis revealed a positive correlation between serum OPG and the severity of cerebral microbleeds(rs=0.736,P＜0.05)and a negative correlation between calcium and the severity of cerebral microbleeds(r,=-0.752,P＜0.05).No significant differ-ences were observed in serum OPG and calcium levels between patients with cerebral microbleeds in different locations(P＞0.05).Conclusion Elevated serum OPG and reduced calcium levels are observed in patients with acute ischemic stroke and cerebral microbleeds.The levels of serum OPG and calcium are closely related to the severity of cerebral microbleeds,and early combined detection can effectively predict the risk of cerebral microbleeds.

Objective To investigate the correlation of serum osteoprotegerin(OPG)and calci-um levels with cerebral microbleeds in patients with acute ischemic stroke.Methods A total of 97 patients with acute ischemic stroke were selected as the study subjects and divided into cerebral mi-crobleed(group 31 patients with)and non-cerebral microbleed(group 66 patients)based on the re-sults of susceptibility-weighted imaging.Demographic data and laboratory examination indicators were collected from the two groups,and serum OPG and calcium levels were measured.The levels of serum OPG and calcium were compared between patients with different degrees of lesion and bleeding sites.Spearman rank correlation analysis was used to determine the correlations of serum OPG and calcium with cerebral microbleeds.Multivariate Logistic regression analysis was conducted to explore the influ-encing factors of cerebral microbleeds.Receiver operating characteristic(ROC)curves were plotted to assess the predictive value of serum OPG and calcium for cerebral microbleeds.Results Significant differences were observed in age,proportions of patients with drinking and hypertension as well as diabetes,systolic blood pressure,diastolic blood pressure,and serum OPG and calcium levels be-tween the cerebral microbleed group and the non-cerebral microbleed group(P＜0.05).Multivari-ate Logistic regression analysis revealed that older age,history of alcohol consumption,history of hy-pertension,high systolic blood pressure,and high OPG level were independent risk factors for cere-bral microbleeds(OR=1.480,1.330,1.843,1.632,1.652;P＜0.05),while high calcium level was an independent protective factor for cerebral microbleeds(OR=0.721,P＜0.05).The AUC values for the prediction of cerebral microbleeds in acute ischemic stroke patients using serum OPG or calcium alone,and their combination were 0.853,0.825,and 0.921,respectively,with the combined prediction showing higher value than the individual prediction(Z=2.895,3.138;P＜0.05).Spearman rank correlation analysis revealed a positive correlation between serum OPG and the severity of cerebral microbleeds(rs=0.736,P＜0.05)and a negative correlation between calcium and the severity of cerebral microbleeds(r,=-0.752,P＜0.05).No significant differ-ences were observed in serum OPG and calcium levels between patients with cerebral microbleeds in different locations(P＞0.05).Conclusion Elevated serum OPG and reduced calcium levels are observed in patients with acute ischemic stroke and cerebral microbleeds.The levels of serum OPG and calcium are closely related to the severity of cerebral microbleeds,and early combined detection can effectively predict the risk of cerebral microbleeds.

Objective To explore artificial reconstruction vs artificial intelligence reconstruction in coronary artery volume rendering(VR).Methods A total of 110 coronary heart disease(CHD)patients were enrolled in the study,and underwent computed tomography arteriography.Artificial reconstruction(artificial group)and artificial intelligence reconstruction(intelligent group)were conducted in coronary artery VR.The image quality of VR and curved planar reconstruction(CPR),the diagnosis coincidence rate of arterial stenosis,and vascular reconstruction score were compared between two groups.Results The VR image quality of intelligent group was significantly higher than that of artificial group,with excellence rate of 90.91%vs 80.00%;and intelligent group scored higher than artificial group in CPR image quality(P<0.05).The differences in the diagnostic coincidence rates of arterial vessels(RCA,LAD,LCX)with no stenosis,slight stenosis,moderate stenosis,and vascular occlusion between two groups were trivial(P>0.05),while the two groups differed significantly in the diagnosis coincidence rates of mild stenosis and severe stenosis(P<0.05).The mean total score,the median value of the 3 main branches+posterior descending branch+intermediate branch+diagonal branch+blunt margin branch,and the median value of the other vascular branches were all significantly increased in intelligent group as compared with artificial group(P<0.05).Conclusion Artificial intelligence reconstruction has higher application value than artificial reconstruction in coronary artery VR.