0.05) from that of the cryoprecipitate, but was shorter than that in the FFP and frozen platelet. Conclusion The clinical use of the platelet cryoprecipitate suspension is superior to that of common blood components, because it includes platelets and coagulable factors.

Objective To study the effects of aircraft, railway and highway transportations on the quality of red cell suspension.Methods Red cell suspensions from aircraft, railway and highway transportations were randomly selected, 10 units each, and stored for about 20 days as test groups, Another 10 unites of red cell suspensions were collected and stored for 20 days to serve as the control group. Biochemical indicators, including lactic dehydrogenase(LDH), Na+, K+ and Cl-were tested by automatic biochemistry analyzer. Free hemoglobin (FHb) was measured by chromatometry of 3 wavelengths. PO2 was detected by blood gas analyzer. The rate of red blood anaerobic glycolysis and osmotic fragility were also tested. The microstructure of red blood cells (RBCs) was observed by scanning electron microscope.Results The rates of anaerobic glycolysis of aircraft and railway transported red cell suspensions were (42.9?5.6)% and (42.2?3.1)%, respectively, significantly lower than those of highway transportation and the control group, which were (60.4?5.9)% and (70.1?7.8)%, respectively. FHb concentrations in erythrocyte suspension from aircraft and railway transportations were (1.37?0.31) mg/ml and (1.28?0.41) mg/ml, respectively, significantly higher than those of the highway transportation and the control group, which were (0.37?0.05) mg/ml and (0.31?0.03) mg/ml, respectively. LDH level and Na+ concentrations in erythrocyte suspension from aircraft and railway transportations were significantly higher than those from highway transportation and the control group. PO2 was decreased significantly in red cell suspension from aircraft transportation, and the osmotic fragility was increased. K+ was increased significantly in red cell suspension from railway transportation. Cl-was not changed. The aircraft and railway transported RBCs showed agglutination, spherical changes and ragged edges. Many thorn RBCs could be found in these samples. The morphology of RBCs after highway transportation had normal shape , but a few were spherical and ragged at edges. The RBCs of the control group was in double-faced concave disc shape and equably suspended.Conclusion The quality of red cell suspension is closely related to the different ways of transportation. Under certain circumstances, the quality of red cell suspension from highway transportation is superior to that from aircraft and railway transportations.

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous cell population of myeloid origin with immunosuppressive function. MDSCs can inhibit the normal immune response of the host to tumor by inhibiting the activity of T cells, natural killer cells, macrophage, B cells, and promoting the proliferation of Treg and other mechanisms. Therefore, MDSCs are potential targets for tumor immunotherapy. However, whether immunotherapy or other treatments, the effectiveness of monotherapy is limited, so immunotherapy combined with other treatments is a breakthrough combination. In recent years, immunotherapy combined with other treatments has shown initial results, with better results than monotherapy. It has become a research hotspot in tumor treatment and a new treatment method in the future. This article summarized the research progress on the effects of immunotherapy combined with chemotherapy, radiotherapy, Chinese herbal extract and Immunotherapy combination on MDSCs, in order to provide theoretical support for the clinical application of immunotherapy based combined tumor treatment measures.

Objective To investigate the effect of Angelica polysaccharide(APS)on the differentiation and function of M2 macrophages and underlying molecular mechanism.Methods Mouse bone marrow derived macrophages(BMDM)and M2 macrophages were induced and treated with APS(0,80,160,320 μg/mL);Mouse peritoneal macrophages were isolated and treated with APS(0,160 μg/mL).Flow cytometry(FCM)was used to detect mannose receptor(MR),CD11b,F4/80,CD163,and ARG-1 expression levels,apoptosis,and phagocytic ability of M2 macrophages and peritoneal macrophages.Mice were randomly divided into APS gavage group and control group,APS was intragastrically administered to mice,and macrophage MR expression level in blood and spleen were detected by FCM.Fluorescence microscopy was used to observe the morphology of BMDM-differentiated M2 macrophages.RT-qPCR was employed to detect the mRNA expression levels of MR and ARG-1 in M2 macrophages.Immunofluorescence assay was performed to detect the expression of the proteins related to molecular mechanism of differentiation and function of M2 macrophages.Results Compared with the 0 μg/mL APS group,the MR expression level in the M2 macrophages was decreased with the increase of APS concentration within a certain concentration range(80～320 μg/mL),and the MR expression level in peritoneal macrophages was also decreased in the 160 μg/mL APS treatment group(P＜0.01).The expression level of macrophage MR was also significantly decreased in peripheral blood and spleen in the APS gavage mice than the control group(P＜0.05).Compared with the 0 μg/mL APS group,the expression levels of CD11b,F4/80,and CD163 in the macrophages were increased in the 80～320 μg/mL APS treatment groups(P＜0.01).The morphology of macrophage had changed,from mostly spindle-shaped and pseudopodia to mostly round or irregular,and even a few cells with pseudopodia.APS induced apoptosis in M2 macrophages(P＜0.05).Compared with the 0 μg/mL APS group,M2 macrophages treated with 160 μg/mL APS had an increased ability to phagocytose fluorescent microspheres(P＜0.01),but the expression level of ARG-1 was decreased(P＜0.01).The mRNA expression of MR and ARG-1 in M2 macrophages was decreased(P＜0.05).The mean fluorescence intensity of phosphate acidified-signal transducers and activators of transcription 6(p-STAT6)-positive signals in M2 macrophages was significantly reduced in the 160 μg/mL APS-treated group(P＜0.05).Conclusion APS has bidirectional regulation on the differentiation and function of M2 macrophages,which may be associated with its downregulation of signal transducers and activators of transcription 6(STAT6)signaling pathway.

Autologous ozonized blood transfusion(AOBT) is a therapy of re-transfusion of 100-200 mL of autologous blood after shaking and agitation with appropriate amount of oxygen-ozone in vitro. The oxidation of blood through the strong oxidation of ozone can enhance the non-specific immune response of the body, regulate the internal environment and promote health. This therapy has been increasingly applied in clinical practice, while no unified standard for the operation process in terms of ozone concentration, treatment frequency and treatment course had been established. This operation process of AOBT is primarily explored in order to standardize the operation process and ensure its safety and efficacy.