1. Clinical value of prenatal ultrasound in diagnosis of vasa previa
Chinese Journal of Medical Imaging Technology 2019;35(11):1698-1701
Objective: To explore the clinical value of prenatal ultrasound in diagnosis of vasa previa. Methods: The images of 65 230 pregnant women who underwent prenatal ultrasound examination were analyzed retrospectively. The accuracy of prenatal ultrasound in diagnosis of vasa previa was calculated. The delivery modes of all pregnant women and outcomes of all perinatal infants with vasa previa diagnosis were followed up. Results: Fifty-three vasa previa cases were diagnosed during the first examination using prenatal ultrasound and 10 cases were eliminated during reexamination in late pregnancy, so 43 cases were diagnosed. Finally 41 cases (41/65 230, 0.06%) were clinically confirmed. The accuracy of middle pregnancy screening (15-28 weeks) and late pregnancy (28-40 weeks) reexamination was 100%(28/28), and the accuracy of late pregnancy was 86.67%(13/15). Cesarean section surgery was performed in all 41 pregnant women, and all fetuses survived. Conclusion: Prenatal ultrasound has a high value of prenatal ultrasound in diagnosis of vasa previa.
2.Effects of high mechanical index ultrasound of radiation microbubble on the colon cancer cell skeletal
Hua ZHONG ; Rui LI ; Yanli GUO ; Xing HUA ; Xiaohang ZHANG ; Yafang XUE ; Zhaohui CHEN
Chinese Journal of Ultrasonography 2011;20(11):996-999
Objective To Study the effects of high mechanical index ultrasound radiation microbubble on colon cancer cell skeletal.Methods Lovo cell were cultured in vitro,and this study was divided into control group,simple contrast agent group,microbubble plus ultrasound radiation group and simple ultrasound radiation group.Ultrasound contrast agent SonoVue and ultransound (frequency 1.5 MHz and MI 1.7) were used.Results In the control group,the microtubes were densest in dyeing silk,and extended to the edge of a cell.In the ultrasound and microbubble group,the microtubes were weaker and thin,the.network was mainly in the structure of the long axis.The cells arranged in a slight pigmentation to the density cell,to reach out many fine short hair,there were obviously a sense of silk and directional.In the microbubble and ultrasound group,Lovo cells streamed of the central significantly reduced,fluorescent lighting,with short hair.There was no difference in the ultrasound group and the microbubble group with microfilament and microtubule.Conclusions High mechanical index ultrasound radiation can changed into vacant,a trace of the assembly and distributed to the tumor cells are attacking,the transfer of restraining.
3.Effects of high mechanical index ultrasound contrast on the hepatic metastasis of cancer
Hua ZHONG ; Rui LI ; Yingxue HAO ; Yanli GUO ; Xing HUA ; Yafang XUE ; Zhaohui CHEN
Chinese Journal of Ultrasonography 2008;17(5):430-433
Objective To observe the effects of high mechanical index ultrasound contrast on hepatic metastasis of colorectal cancer. Methods Hepatic metastasis models were established by injecting Lovo cells into the spleen of SD rats.This study was divided into control group, microbubble plus ultrasound radiation group simple contrast agent group and simple ultrasound radiation group.Ultrasound contrast agent SonoVue(1 ml/kg) was injected via tail vein, high mechanical index ultrasound contrast (frequency 1.5 MHz,MI 1.7) was performed intermittently for 2 min.After 10 d,the animals were killed and the size and number of hepatic metastasis were compared.Histological pathology and its ultrastructure were observed.Results There were no significant differences between contral group,simple ultrasound radiation group and simple contrast agent group in metastatic rate ( P>0.05).There were no obvious differences between contral group, simple ultrasound radiation group and simple contrast agent group in cells'ultrastructure by transmission electron microscopy (TEM).The number and size of hepatic metastasis decreased obviously in microbabble plus ultrasound radiation group ( P<0.01 ), the mitochondrion was swelling and the gaps of endothelial cells were loose. Conclusions High mechanical index ultrasound contrast can inhibit the hepatic metastasis of colorectal cancer.
4.The value of prenatal ultrasound in diagnosis of fetal meningocele or encephalocele
Fenglian DENG ; Yanli GUO ; Lingmin DUAN ; Yujie FENG ; Hua ZHONG ; Jianjun LIU ; Yafang XUE
Chongqing Medicine 2017;46(4):475-477
Objective To investigate the clinical value of prenatal ultrasound in fetal meningocele and encephalocele.Methods Thirty nine fetuses with meningocele and encephalocele confirmed by prenatal ultrasound and abortion were acquired and their clinical data and sonographic features were retrospectively analyzed.Results Ultrasound screening in 111 620 cases showed 39 fetuses were with meningocele and encephalocele,accounting for 0.35%.Among them,16 cases were diagnosed with meningocele (including 1 case with two bulging parts) and 23 cases with encephalocele.Prenatal ultrasound could clearly detect the location and size fetal skull defect,and bulging features.According to their sonographic features,meningocele or encephalocele was determined.Conclusion Ultrasound could be a reliable prenatal screening method,provide an important basis for clinical intervention and have a significant clinical value in fetal meningocele and encephalocele.
5.Prenatal Ultrasonography in Assessing Limb Body Wall Complex at Gestation
Fenglian DENG ; Lingmin DUAN ; Yanli GUO ; Xiaohang ZHANG ; Rui LI ; Yafang XUE ; Jianjun LIU
Chinese Journal of Medical Imaging 2014;(11):856-859
PurposeTo explore the value of prenatal ultrasound in diagnosis of fetuses with limb body wall complex (LBWC).Material and MethodsThe ultrasound images and follow-up results were studied retrospectively in 20 cases of LBWC, and the ultrasonic features of LBWC were analyzed.ResultsAll 20 cases showed rather severe body wall defect and spinal abnormality, including 16 cases of abdominal wall defect, 4 cases of abdominal thoracoabdominal defect, 8 cases of idiopathic scoliosis, 2 cases of kyphosis, 3 cases of twist into a corner, 1 cases of distortion as S shape, and 6 cases of vertebral body arranged in disorder. Moreover, 16 cases presented abnormal umbilical cord,among which 11 had too short umbilical cord, 1 without umbilical cord, and 7 as single umbilical artery (3 cases with short cord). Five cases showed limb abnormalities, among which 2 cases were left lower limb absence, 1 cases as left upper limb absent, 1 cases as left foot varus and right foot slightly varus, and 1 cases as left foot drop.ConclusionPrenatal ultrasonography can accurately diagnose LBWC in time, so as to provide evidence for early clinical intervention.
6.Effects of subcellular localization of neurotrophin receptor p75 interacting MAGE homologue on the epithelial to mesenchymal transition and radioresistance of esophageal cancer cells
Yafang ZHANG ; Ge ZHANG ; Xiaojing CHANG ; Huandi ZHOU ; Xiaohui GE ; Xiaoying XUE
Chinese Journal of Radiological Medicine and Protection 2016;36(3):186-190
Objective To investigate the role of NRAGE subcellular localization in the EMT and radioresistance of esophageal cancer cells.Methods EMT model cells were established by the treatment of TE13 cells with TGF-β1.To verify the establishment of EMT model and the phenotype of EMT-like TE13R120 cells,EMT marker mRNA and protein were detected by Real-time PCR and Western blot,respectively.Real-time PCR was also used to detect the expression of NRAGE mRNA in three groups.Total NRAGE protein,cytoplasm protein and nuclear protein were measured by Western blot.Results It was found that TGF-β1 could induce morphological alterations of TE13 cells from epithelial to mesenchymal and change the expressions of EMT maker E-cadherin and vimentin (t =13.56,-232.84,P < 0.05),indicating the successful establishment of EMT model cells.Similar expression trends of EMT makers were observed in TE13R120cells (t=15.84,-54.54,P<0.05).NRAGE mRNA (t=-8.73,-5.62,P< 0.05) and total protein in both EMT model cells and TE13R120 cells were higher than that in TE13 cells,especially for the nuclear proteins.However,no differences in NRAGE cytoplasm protein expression were found among the three groups.In addition,there were also no difference of NRAGE mRNA (t =-0.88,P >0.05),cytoplasm and nuclear protein between TE13R120 cells and EMT model cells.Conclusions The radioresistant cell line TE13R120 has the EMT-like phenotype that may cause cell radioresistance by changing the subcelluar localization of NRAGE.
7.Comparative study on fetal ultrasonic diagnosis and pathological results of complex congenital heart diseases
Xiaohang ZHANG ; Xiaodong GE ; Rui LI ; Ping ZHANG ; Lingmin DUAN ; Yanli GUO ; Yafang XUE ; Xiaolin LI ; Shuhong GAO ; Xiuwu BIAN
Chinese Journal of Ultrasonography 2010;19(6):517-520
Objective To probe the diagnostic efficacy of fetal eehocardiography for characterizing complex congenital heart diseases.Methods Fetal echocardiography was performed on 49 cases of fetal complex congenital heart disease, the ultrasonic diagnosis was compared retrospectively with pathological results after autopsy.Results Antenatal sonographic diagnosis was in agreement with the pathological results in 42 cases (85.71 %), 7 cases were disagreed with pathological diagnosis (antenatal sonographic diagnosis was discrepancy in 3 cases, 4 cases were partially mis-classified).Twenty-four cases were combined with extra-cardiac malformations.Nine cases had chromosomal abnormality.Conclusions Fetal echocardiography is highly accurate for antenatal diagnosis of complex congenital heart disease, but it is hard to detect some type of cardiac anomalies.
8.Three dimensional fetal heart structure study in fetal heart by sono-automatic volume count with spatio-temporal image correlation
Xiaohang ZHANG ; Rui LI ; Lingmin DUAN ; Yafang XUE ; Xuemei ZHANG ; Jianjun LIU ; Yan WANG ; Yanli GUO ; Hua ZHONG
Chinese Journal of Ultrasonography 2011;20(1):41-43
Objective To evaluate three dimensional fetal heart structure by sono-automatic volume count (SonoAVC) with spatio-temporal image correlation(STIC). Methods The heart volumes datas were acquired by STIC in 32 fetuses with postnatally confirmed diagnoses(20 cases of normal heart, 12 cases of complex congenital heart disease between 20 - 37 gestional weeks), then the volume datas were analyzed offline. SonoAVC software automaticly searched hypoechoic and anechoic structures, and assigned individual colors,and this can be corrected by manual splitting and/or removing and merging of individual segments.Results Individual segments of fetal heat could be separated,and digital casts were generated. The digital casts were obtained successfully in 4cases of transposition of the great arteries(TGA) ,2 cases of tetralogy of Follot,1 case of Ebstein's anomaly, 1 case of double-outlet right ventricle and 20 cases of normal heart.Conclusions Combination of STIC and SonoAVC can demonstrate the size, shape and connection of fetal cardiac cavities and great arteries in three dimensional spatial context. It has the potential both to help in obtaining strctural diagnostic,and to generate 3D visual displays for consultation and teaching.
9.Clinical and experimental studies on five cases of acute myeloid leukemia with translocation t(16;21)(p11;q22).
Yafang WU ; Yongquan XUE ; Jinlan PAN ; Qinfeng MA
Chinese Journal of Medical Genetics 2003;20(2):171-173
OBJECTIVETo report five cases of acute myeloid leukemia (AML) with t(16;21)(p11;q22) translocation and the result of chromosome painting analysis on one of them.
METHODSChromosome specimens were prepared by short-term culture of bone marrow cells. Karyotype analysis was made by R-banding technique. Chromosome painting was performed using whole chromosome probes 16 and 21 in 1 case.
RESULTSKaryotype analysis showed identical translocation t(16;21)(p11;q22) in all five cases, accounting for 0.3% of 1448 cases of acute myeoid leukemia examined in the past fifteen years. Moreover, chromosome painting distinctly demonstrated t(16;21) in one of them. Leukemia blasts did not show hemophagocytosis in all of them.
CONCLUSIONt(16;21) translocation is a rare and recurring chromosome rearrangement. It represents a specific type of AML. Chromosome painting technique is a more reliable means for detecting it, compared with the conventional karyotype analysis.
Acute Disease ; Adolescent ; Adult ; Cells, Cultured ; Child ; Chromosomes, Human, Pair 16 ; genetics ; Chromosomes, Human, Pair 21 ; genetics ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Karyotyping ; Leukemia, Myeloid ; genetics ; Male ; Translocation, Genetic
10.A preliminary study on the early diagnosis of myelodysplastic syndromes.
Jun QIAN ; Yongquan XUE ; Fei YU ; Yafang WU ; Jinlan PAN ; Dingwei LU
Chinese Journal of Hematology 2002;23(6):307-310
OBJECTIVETo evaluate the four techniques for clonal analysis in the early diagnosis of myelodysplastic syndromes (MDS).
METHODSFour techniques for clonal analysis were performed in bone marrow samples from fifty patients with suspected MDS: (1) Conventional cytogenetics (CC) for clonal chromosomal abnormalities; (2) BrdU-sister chromatid differentiation (BrdU-SCD) for cell cycle analysis; (3) Fluorescence in situ hybridization (FISH) for trisomy 8; (4) PCR-SSCP for N-ras mutation.
RESULTSThe diagnosis of forty-five patients was compatible with FAB criteria of MDS, the other five patients didn't fully meet the FAB criteria. They had either only one lineage dyspoiesis or no any obvious dysplastic features and two of them were diagnosed as suspicious refractory anemia (RA), one as anemia with hypercellular bone marrow and two as chronic aplastic anemia. The results of the four techniques performed in them showed that four patients had clonal karyotype abnormalities, two had prolonged cell cycle, three had trisomy 8 of different proportions, and one had N-ras mutation. Thus, they were all diagnosed as RA.
CONCLUSIONThe untypical MDS patients can be diagnosed early by examination with combining several clonal analysis techniques.
Adolescent ; Adult ; Aged ; Bromodeoxyuridine ; Child ; Child, Preschool ; Chromatids ; Chromosome Aberrations ; Cytogenetic Analysis ; methods ; Female ; Genes, ras ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Male ; Middle Aged ; Mutation ; Myelodysplastic Syndromes ; diagnosis ; genetics