Objective To explore the diagnostic value of multi-slice computer tomography (MSCT) in cause and location of agnogenic intestinal obstruction. Methods With light speed 16 multiple slice spiral-CT, 68 patients clinically suggested intestinal obstruction were underwent plain scan and second phase enhanced scan in whole abdomen. The image data was post-processed through GEADW4. 4 station with multi-planar reconstruction (MPR) and maximum intensity projection (MIP). The CT image characters of agnogenic intestinal obstruction were analyzed by comparing CT image suggestions with operation findings. Results MSCT clearly indicate the location, cause and severity of intestinal obstruction, and the diagnose accordance rate with operation findings was 95.6 % (65/68). Conclusions MSCT is a quick, simple and effective means for diagnosis of the location,causes and severity of agnogenic intestinal obstruction. It could provide an important clue for the doctors when making clinical decisions.