Objective: To construct a vector encoding short hairpin RNA(shRNA) targeting annexin H and to observe its influence on annexin II expression. Methods: Annexin II-targeted hairpin RNA1 and RNA2 were devised according to the Gen-Bank annexin II sequence. Meanwhile, a nonspecific sequence was taken as negative control. The oligonucleotide strands of DNA fragments encoding the above shRNAs were synthesized. After annealing of the complementary strands, the DNA fragments were cloned into human pGenesil1 plasmid followed by amplification in E. coli and sequencing. The 3 pGenesil1 constructs were then transfected into HEK293 cells and the expression of annexin II was examined by real-time fluorescence quantitative RT-PCR and Western bolt. The non-transfected cells were taken as blank control. Results: Restrictive enzyme digestion and sequencing verified that the 3 recombinants (pGenesil1-annexin II shRNA1, pGenesil1-annexin II shRNA2, and pGenesil1-negative shRNA) were correct. The annexin II expression was significantly lower in HEK293 cells transfected with pGenesil1-annexin II shRNA2 compared with those transfected with pGenesil1-annexin II shRNA1, pGenesil1-negative shRNA, and blank control (P<0.05); and there was no significant difference between the latter 3. Conclusion: We have successfully generated an annexin II-targeted shRNA construct, which can significantly inhibit the expression of annexin II.

Objective: To separate human kidney phosphoproteome by two-dimensional gel electrophoresis. Methods: The phosphorylated proteins from human kidney tissues were enriched with phosphate metal affinity chromatography (PMAC) resin. After being concentrated and desalted, the samples were separated by isoelectric focusing on first dimension and SDS electrophoresis on second dimension. Results: The phosphorylated proteins were successfull y extracted from human kidney tissues and were separated by two-dimensional gel electrophoresis. Conclusion: Phosphoprotein enrichment technique combin ed with two-dimensional gel electrophoresis is an effective approach to study phosphoproteome, laying a foundation for further investigation of human kidney phosphoproteins.

Objective To evaluate the clinical value of multi-slice spiral CT in diagnosing tracheobronchial foreign bodies in chil-dren.Methods 139 patients with airway foreign body confirmed by bronchoscopy were examined with 128-slice spiral CT,and then the CT data was reconstructed by using multiple-planar reconstruction(MPR),curved planar reconstruction (CPR),volume render-ing(VR),minimum intensity projection(MinIP)and virtual endoscopy (VE).The CT results were compared with bronchofibersco-py findings.Results Among the 139 cases,the foreign bodies located at trachea in 13 cases,right bronchia in 75 cases,left bronchia in 48 cases,and bilateral bronchia in 3 cases.137 cases (98.6%)were correctly diagnosed,and 2 cases (1.4%)were missed on CT.Although the difference of the results at trachea and right main stem bronchus was statistically significant (P <0.05),the over-all results was not significant difference between CT and bronchoscopy examination (P =0.340).Associated findings included em-physema in 74 cases,pneumonia in 48 cases,atelectasis in 22 cases,mediastinal displacement in 57 cases,lung interstitial emphyse-ma in 4 cases,pneumomediastinum and pneumoderma in 4 cases,bronchiectasis in 2 cases,and pleural effusion in 4 cases.Conclu-sion MSCT and its postprocessing techniques can accurately and intuitively display the tracheobronchial foreign body and complica-tions,which has important value for clinical application.

Adult ; Autopsy ; methods ; Female ; Humans ; Plasma Exchange ; Purpura, Thrombotic Thrombocytopenic ; pathology

DNA Repair ; genetics ; DNA-Binding Proteins ; genetics ; Drug Resistance, Multiple ; Drug Resistance, Neoplasm ; Endonucleases ; genetics ; Humans ; Neoplasms ; drug therapy ; genetics ; Nuclear Proteins ; genetics ; Organoplatinum Compounds ; therapeutic use ; Platinum Compounds ; therapeutic use ; Polymorphism, Single Nucleotide ; Transcription Factors ; genetics ; X-ray Repair Cross Complementing Protein 1 ; Xeroderma Pigmentosum Group D Protein ; genetics

The tools used for the literature quality evaluation are introduced. The common evaluation tools that are publicly and extensively used for the evaluation of clinical trial literature quality in the world are analyzed, including Jadad scale, Consolidated Standards of Reporting Trials (CONSORT) statement and Grades of Recommendations Assessment, Development and Evaluation (GRADE) system and the others. Additionally, the present development, updates and applications of these tools are involved in analysis.
Biomedical Research ; standards ; Evaluation Studies as Topic ; Humans ; Publications ; standards ; Quality Control

A CDASH-based CRF annotation plays an important role in database setup and data verification. The STDM-based CRF annotation is also one of the essential documents when the package of clinical trial data is submitted to the regulatory authority. This paper describes the contents, procedures and related stipulations used in the CDISC-based CRF annotation.
Clinical Trials as Topic ; Databases, Factual ; Documentation ; standards

Objective To explore the relation ship between expression of survivin gene in leukemia cells and its clinical effects, and to study the mechanism of survivin resist-apoptosis function in leukemia.Methods Survivin expression was detected by Western blots analysis and expressions of Fas and Caspase were examined by immunohistochemistry in 18 leukemia patients.Results Thirteen cases in peripheral blood mononuclear cell survivin positive expression was detected in 18 leukemia patients(72.2%), but no survivin expression in 10 normal persons. There were significant difference of survivin expression in ALL/ANLL patients groups and different WBC groups(P

Objective To study the rationality and possibility of 64 slice spiral CT in the examination of the temporal bone at low dose.Methods The same CT technique and temporal bone mode as those for clinical CT were used,two cranium specimens(four ears)were scanned with Somatom Sensation 64-slice spiral CT at different mA(380,300,200,160,120,80),and muhi-planar reformation was performed.The CT dose index at different mA groups were measured by 10 cm pencil ionization chamber and head dose phantom.Four anatomic structures on axial images(subarcuate fossa,tendon of tensor tympani, facial recess,etc),four anatomic structures on coronal images(scute,crista transversa,fenestra cochleae, etc)and six anatomic structures on double oblique images(malleus,incus,stirrup bone,upper bony semicircular,etc)were chosen to evaluate and grade the reformation images among different mA groups,and to determine the minimum mA value.Ten ears of five patients were used to test the validity of the minimum mA value.Results CT radiation dose was significantly reduced from(47.8?2.7)to(20.1?2.0)raCy (P

Bronchogenic Cyst ; pathology ; surgery ; Diagnosis, Differential ; Gastrectomy ; methods ; Humans ; Male ; Middle Aged ; Stomach Diseases ; pathology ; surgery