1.Efficacy of jaw thrust device in the upper airway obstruction
Chinese Journal of Anesthesiology 2009;29(9):814-815
Objective To evaluate the efficacy of jaw thrust device in the upper airway obstruction. Methods Thirty-five ASA Ⅰ or Ⅱ patients aged 18-64 yr with body mass index < 30 kg/m~2 undergoing elective surgery under general anesthesia were included in this study. The anesthesia face mask was placed and held tightly by 4 straps of JTD and connected to the anesthesia machine. Anesthesia was induced with midazolam, propefol, fentanyl and atracurium. When muscle relaxation was achieved, tracheal intubation was not performed. The airway obstruction was assessed by airway peak pressure and stridor score (breathing sounds detected by auscultation over the trachea). And then tracheal intubation was performed, and the patients were mechanically ventilated. Results There was no significant difference in airway pressure and stridor scores between JTD and jaw thrust maneuver. Conclusion JTD can effectively lift the jaw and improve the upper airway obstruction.
2.Discussion on Laboratory Teaching of Medical Microbiology to Foreign Students
Chinese Journal of Medical Education Research 2002;0(01):-
In this paper,the teaching in medical microbiology experiment to the first group of foreign students in our college is analyzed and summarized,which will give us many experiences for later teachering.
3.Investigation of the API distribution homogeneity in lyophilized product-pemetrexed disodium for injection by confocal micro-Raman spectroscopy mapping
Jing HAN ; Jing YAO ; Mei-yang DONG ; Ya-qin SHI ; Jia-bei SUN
Acta Pharmaceutica Sinica 2022;57(7):2158-2165
The method of homogeneity evaluation for active pharmaceutical ingredient (API) spatial distribution in lyophilized product was investigated for the first time with confocal micro-Raman spectroscopy mapping, using pemetrexed disodium for injection as a model drug. Certain areas of the lyophilized product were scanned to obtain Raman spectra. The classical method ("peak clipping" method) was employed for mapping with characteristic Raman peaks of the API and the excipient. Due to the API being finely dispersed in the excipient in lyophilized products, the classical method cannot discriminate between the two ingredients making the distribution homogeneity difficult to evaluate. The "ratio of characteristic peak intensities" method was then utilized. Using this method, the relative intensity of the characteristic Raman peaks of the API to the excipient was applied for mapping and the relative content of API to excipient was calculated for a homogeneity evaluation of the drug distribution. The validation of this method showed a good linear relationship between the relative intensity and the relative content of API to excipient (
5.Chloroplast genetic engineering: a new approach in plant biotechnology.
Tao SU ; Ya-Guang ZHAN ; Mei HAN ; Ai-Ping HAO
Chinese Journal of Biotechnology 2005;21(4):674-680
Chloroplast genetic engineering, offers several advantages over nuclear transformation, including high level of gene expression, increased biosafety, remedying some limitations associated with nuclear genetic transformation, such as gene silencing and the stability of transformed genes. It is now regarded as an attractive new transgenic technique and further development of biotechnology in agriculture. In this article we reviewed the characteristics, applications of chloroplast genetic engineering and its promising prospects were discussed.
Biotechnology
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methods
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Chloroplasts
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genetics
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Genetic Engineering
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methods
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Plants, Genetically Modified
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genetics
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Transformation, Genetic
6.Experimental observation of articular impairment of New Zealand rabbits by deoxynivalenol
Qun-wei, LI ; Hai-feng, HOU ; Xiao-mei, LI ; Ya-lu, LI ; Ji-ju, HAN ; Xin-nong, WANG
Chinese Journal of Endemiology 2009;28(1):41-43
Objective To observe toxic effect of deoxynivalenol(DON)on articular cartilage and synovium of New Zealand rabbits's knee ioints.Methods Fifteen male rabbits were divided randomly into 3 groups:control, high-dosage,and low-dosage group.In high-dosage and low-dosage group,saline solution of DON was injected with a dose of 0.10 and 0.05 ms/kg every 48 h into ear vein of rabbits.Specimen of articular cartilage and synovium were through pathologY methods,and IL-1β,TNF-α,NO levels were assayed in joint liquid,after 20 days. Results Morphological changes were observed, such as synovium inflammative infiltration, chondrocytes deformation and necrosis under light microscope.The levels of IL-1β,TNF-α and NO had statistical significance in comDarison between 3 grouPs(F=19.396,18.195,22.136,P<0.05).The levels of IL-1β,TNF-α and NO were significantly higher(all P<0.05),high-dosage[(0.451±0.091),(0.575±0.122)μg/L;(70.27±11.53)μmol/L] and low-dosage group[(0.295±0.107),(0.387±0.131)μg/L;(45.32±12.24)μmol/L]compared with control ((0.1 13±0.049),(0.138±0.087)μg/L;(23.56±9.35)μmoL/L],and high-dosage compared with low-dosage group Conclusions DON results in articular and synovial impairment,which has the symptom similar to osteoarthritis. DON probably causes osteoarthritis.
7.Vinorelbine induces apotosis and decreases telomerase activity in human epithelial ovarian cancer cells line SKOV 3
yuan Li SHEN ; mei Dong XU ; han Xiao LIU ; Mei YANG ; ling Ya WEN
Basic & Clinical Medicine 2018;38(1):87-90
Objective To investigate the effect of different concentrations of vinorelbine on apoptosis ,telomerase ac-tivity and expression of human telomerase-reverse transcriptase gene ( hTERT ) in human epithelial ovarian cancer cells SKOV3.Methods Ovarian cancer cells SKOV 3 were treated with vinorelbine under different concentrations . The cell proliferation was measured by cell counting kit-8 ( CCK-8) assay , and the cell apoptosis was detected by flow cytometry.The telomerase activity of SKOV3 cells was determined by TRAP-PAGE-silver staining;The mRNA expression of hTERT was performed by RT-PCR assay .Results Vinorelbine could significantly inhibited the prolifer-ation of SKOV3 cells,induce cell apoptosis (P<0.01),reduce the telomerase activity and expression of hTERT mRNA (P<0.01), in dependent of a concentration-time manner.Conclusions The detection of telomerase activity and the mRNA expression of hTERT might be vital for predicting the prognosis of patients with epithelial ovarian cancer .
8.Genotype and sequence analysis on G2 segments of hantavirus from HFRS patients in Hebei Province.
Qi LI ; Ya-mei WEI ; Zhan-ying HAN ; Yan-bo ZHANG ; Shun-xiang QI ; Yong-gang XU
Chinese Journal of Experimental and Clinical Virology 2008;22(1):15-17
OBJECTIVETo know the genotype and subtype of hantavirus (HV) which infected persons in Hebei province.
METHODSAccording to G2 coding region of 76-118 and R22 strains, specific type primers were designed to detect and identity the types of HV in HFRS patients' sera with RT-nested PCR. Nucleotides were assayed from partial products after purification and reclaim. Then, gene analysis was done with DNAStar package.
RESULTS17 out of 69 positive serum specimens were successfully detected by RT-PCR and the detection rate was 24.64%, among which,
CONCLUSIONSEO was the major type of HV from HFRS patients in Hebei province, S3 was the major subtype and S1 was also existed. In a certain area, the HV which belonged to the same type was correspondingly conservative, and had the characteristic of regional stability.
China ; Genotype ; Hantavirus ; classification ; genetics ; Hemorrhagic Fever with Renal Syndrome ; diagnosis ; prevention & control ; therapy ; virology ; Humans ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Viral Envelope Proteins ; genetics
9.Genotyping and sequence analysis on G2 segment of hantavirus carried by rat in Hebei province.
Xiao-Xia HUANG ; Qi LI ; Zhan-Ying HAN ; Yan-Bo ZHANG ; Ya-Mei WEI ; Yong-Gang XU
Chinese Journal of Experimental and Clinical Virology 2009;23(3):165-167
OBJECTIVETo know the genotype and subtype of hantavirus (HV) carried by host animals in HFRS natural epidemic area of Hebei province.
METHODSAccording to the conservative sequence of 76-118 and R22 strains, specific primers were designed. RT-nested PCR was used to amplify partial M segments from the positive rat lungs screened by IFA. Agarose gel electrophoresis was used to identify the types. Nucleotides were assayed from partial products after purification and reclaim. Gene analysis was carried on with DNAStar package.
RESULTS32 specimens, which were positive screened by IFA, were amplified the specific segment (418bp) and all belonged to type SEO. Sequencing results of 10 partial segments indicated that G2 segment had little variability and nucleotide homology reached to 98.0%-100.0%. Comparing with the R22 and 76-118 strains, homology was 93.3%-94.3% and 67.7%-69.0% respectively.
CONCLUSIONAccording to G2 segment, SEO was the major type in Hebei HFRS natural epidemic area and S3 was the major subtype. HV which belonged to the same subtype had high homology and genetic materials were correspondingly stable. Different rats could carry the same subtype of HV.
Animals ; Animals, Wild ; virology ; Disease Reservoirs ; virology ; Genotype ; Hantavirus ; classification ; genetics ; isolation & purification ; Lung ; virology ; Molecular Sequence Data ; Phylogeny ; RNA, Viral ; genetics ; Rats ; virology ; Sequence Analysis, DNA
10.Quality of life and its influencing factors in patients with post-traumatic epilepsy.
Song-Yan LIU ; Xue-Mei HAN ; Ya-Yun YAN ; Bo-Jian SUN ; Ying CHANG
Chinese Journal of Traumatology 2011;14(2):100-103
OBJECTIVETo observe the quality of life in patients with post-traumatic epilepsy and discuss the influencing factors.
METHODSWe assessed 105 patients with post-traumatic epilepsy and 100 healthy people as control using Quality of Life Scale-31 (QOL-31), Self-rating Depressing Scale (SDS) and Self-rating Anxiety Scale (SAS), and conducted retrospective analysis on the depression, anxiety, site of trauma, control of seizure, EEG and therapeutic compliance.
RESULTSPatients with post-traumatic epilepsy scored much lower than the control group on QOL-31 (P less than 0.01), but higher than the control group on SDS and SAS (P less than 0.01). Multiple regression analysis indicated that major influencing factors on the quality of life were anxiety, therapeutic compliance, depression, poor control of epileptic seizure and site of trauma.
CONCLUSIONSThe quality of life in patients with post-traumatic epilepsy has significantly declined. Doctors should pay attention to psychological and mental problems of patients with epilepsy, such as depression and anxiety, enhancing therapeutic compliance and controlling epileptic seizure, which are the keys to improving prognosis.
Epilepsy, Post-Traumatic ; psychology ; Humans ; Quality of Life ; Regression Analysis ; Retrospective Studies