1.Exploration to Innovation on Physical Teaching in Medical Colleges
Ya ZHOU ; Shehua REN ; Xiaohui LI
Chinese Journal of Medical Education Research 2005;0(06):-
Medical physics is the important basic for rapid development of model medical theory and technology.Innovation to physical education prompts the progress of high-level medical education.This study based on relevant educational practice will be beneficial to the progress of high-level medical education through primarily exploring the reforming of educational objective,educational model,educational instrument and educational teaching content.
2.The effect of microwave ablation combined with dendritic cell tumor vaccine treatment on mouse mammary carcinoma and its immunological mechanism
Ya ZHOU ; Shehua REN ; Yongju LI ; Ming ZHANG ; Honglian ZHOU
Chinese Journal of Physical Medicine and Rehabilitation 2014;36(2):91-94
Objective To investigate the therapeutic effects of microwave ablation combined with dendritic cell (DC) tumor vaccine on a murine breast cancer model and to explore its immunological mechanism.Methods Forty-eight Balb/c mice were used to establish models of mouse mammary carcinoma,and then randomly divided into a sham-operation group,a microwave ablation treated group,a DC tumor vaccinated group and a microwave ablation plus DC tumor vaccinated group.Microwave ablation was conducted with the microwave ablation group on day 14 after establishing the model.DC tumor vaccination was performed on day 7 in the vaccinated group.In the microwave ablation plus DC tumor vaccinated group,DC tumor vaccination and microwave ablation were consecutively performed on days 7 and 14,respectively.Tumor growth,lung metastasis and survival were observed.The proportion of CD3+ and CD4+ T cells in splenocytes were analyzed by fluorescence Activated Cell Sorter (FACS) on the 14th day after microwave ablation.Serum levels of interferon-γ (IFN-γ) and interleukin-4 (IL-4) were also detected using ELISA.Results The survival time (56.4 ± 4.3 days),tumor size (67.3 ± 6.1 mm2) and lung metastasis index (0.6 ± 0.4) in the microwave ablation plus DC tumor vaccinated group were all significantly lower than the corresponding averages in the microwave ablation and DC tumor vaccinated group.The proportions of splenic CD3 + T cells (27.8 ±10.3 %) and CD4+ T cells (16.7 ± 8.3 %) in the microwave ablation plus DC tumor vaccinated group were significantly larger than in the control group.Average serum levels of IFN-γ (41.8 ± 22.9 pg/ml) in the microwave ablation plus DC tumor vaccinated group were also significantly larger than in the control group,while the average serum level of IL-4 (15.4± 6.4 pg/ml) was significantly lower.Conclusions Microwave ablation and DC tumor vaccination together could have synergistic effects in treating experimental breast cancer,which might be closely related to the enhanced immune response.
3.The effect of wet hyperthermia combined with microwave ablation on the proportion and function of CD 4 +CD25 +regulatory T cell in animal model of mouse mammary carcinoma
Ya ZHOU ; Yongju LI ; Honglian ZHOU ; Shehua REN
Basic & Clinical Medicine 2015;(9):1188-1193
Objective To investigate the effects of wet hyperthermia combined with microwave ablation on the pro-portion and function of CD 4 +CD25 +regulatory T cell in animal model of mouse mammary carcinoma and explore its significance .Methods An experimental model of mouse mammary carcinoma was established and then treated with microwave ablation on day 14.Local wet hyperthermia was also performed .The proportion of CD4 +CD25 +regula-tory T cells (Tregs) in splenocytes was analyzed by FACS.The relative expression of Foxp3, as well as the expres-sion of CTLA-4 and GITR, on Tregs was determined by real-time PCR assay and FACS respectively .Moreover, the secretion of TGF-βand IL-10 from Tregs also were detected by ELISA .In addition , the suppressive capacity of Tregs on CD4 +CD25 -T cell was also examined by MTT assay .Results The proportion of Tregs in wet hyperther-mia combined with microwave ablation treated group decreased significantly compared with that in wet hyperthermia or microwave ablation treated alone group ( P<0.05 ) .Moreover , the expression level of Foxp 3 on Tregs decreased significantly (P<0.05).Furthermore, the expression of CTLA4 and GITR, as well as the secretion of TGF-βand IL-10, on Tregs also obviously decreased (P<0.05).Finally, the suppressive capacity of Tregs on the proliferation of CD4+CD25-T cells were also significantly impaired ( P<0.05) .Conclusions Wet hyperthermia combined with microwave ablation os more effective than single wet hyperthermia or microwave ablation as a mono -therapy alone on the function of Tregs , which may be closely related to the enhanced suppression of tumor growth .
4.A case report of inborn pyloric duplication.
Li-Qun ZHOU ; Bing-Hui WANG ; Ya-Hua ZUO
Chinese Journal of Contemporary Pediatrics 2007;9(5):421-421
Child
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Female
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Gastroscopy
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Humans
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Pylorus
;
abnormalities
5.Leiomyosarcoma of mediastinum: report of a cases.
Chang-ping LI ; Ya-ru WANG ; Jun-cai ZHOU
Chinese Journal of Pathology 2007;36(1):58-58
Actins
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metabolism
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Adult
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Desmin
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metabolism
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Diagnosis, Differential
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Histiocytoma, Malignant Fibrous
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pathology
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Humans
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Immunohistochemistry
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Leiomyoma
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pathology
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Leiomyosarcoma
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metabolism
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pathology
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surgery
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Male
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Mediastinal Neoplasms
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metabolism
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pathology
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surgery
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Vimentin
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metabolism
6.Effect of vigorous walk on elderly type 2 diabetes.
Hui-min ZHOU ; Bo-ya LI ; Huan ZHU ; Wo NI
Chinese Journal of Applied Physiology 2015;31(3):243-248
7.Small cell carcinoma of the gastric cardia: a histopathological analysis.
Li-qun GONG ; Ya-zhou AO ; Chuan-gui ZHANG
Chinese Journal of Oncology 2006;28(3):216-217
Adult
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Aged
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Carcinoma, Small Cell
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metabolism
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pathology
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therapy
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Cardia
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Chemotherapy, Adjuvant
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Chromogranin A
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metabolism
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Female
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Follow-Up Studies
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Gastrectomy
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methods
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Humans
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Male
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Middle Aged
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Phosphopyruvate Hydratase
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metabolism
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Stomach Neoplasms
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metabolism
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pathology
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therapy
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Survival Rate
8.Primary visceral myopathy: report of a case.
Yu-hua ZHOU ; Ya-bin ZOU ; Miao LI ; Xun SUN
Chinese Journal of Pathology 2012;41(8):568-569
9.Sophoridine inhibits NF-kappaB signaling pathway activation in kidney tissue of endotoxemia mice.
Ling HUANG ; Jianning XIE ; Jinping LIANG ; Yunhong LI ; Ya ZHOU
Acta Pharmaceutica Sinica 2011;46(9):1072-7
This study is to investigate the effects of sophoridine on NF-kappaB signaling pathway in kidney tissue of endotoxemia mice and the mechanism involved. BALB/c mice were challenged with lipopolysaccharide (LPS) caudal vein injection, then sophoridine was administered by intraperitoneal injection. Totally 50 mice were divided into 5 groups: control group, LPS model group, sophoridine treatment 12 mg x kg(-1) group, 6 mg x kg(-1) group and 3 mg x kg(-1) group. All animals were sacrificed at 6 hours after treatment. Kidney and blood samples were harvested. IKKbeta mRNA and TNF-alpha mRNA expression of renal tissue was measured by the reverse transcription polymerase chain reaction (RT-PCR), and phosphorylation IKKbeta protein (pIKKbeta) was detected by immunohistochemistry. NF-kappaB P65 protein expression and distribution of renal tissue were observed by Western blotting and immunofluorescence laser confocal microscopy. Serum TNF-alpha level was detected by radioimmunoassay. The results showed that the sophoridine significantly reduced the expression of IKKbeta mRNA and pIKKbeta protein, and inhibited the expression of NF-kappaB P65 protein and decreased the entry nuclear rate of NF-kappaB P65 in the renal tissue of endotoxemia mice. Thereby the renal TNF-alpha mRNA expression and serum TNF-alpha level were significantly reduced. These results suggest that sophoridine could inhibit inflammatory reaction induced by LPS through inhibiting activation of NF-kappaB signaling pathway.
10.The value of p16 and death-associated protein kinase methylation and autofluroscene spectrum analysis of gastric juice in diagnosing gastric carcinoma
Xiu-E YAN ; Li-Ya ZHOU ; San-Ten LIN ;
Chinese Journal of Digestion 2001;0(11):-
Objective To explore the feasibility of detecting death-associated protein(DAP) kinase promoter hypermethylation in the tumor tissues and plasma of patients with gastric adenocarcino- ma,and to evaluate the effect of DNA hypermethylation and autofluroscene spectrums of gastric juice on diagnosing gastric carcinoma.Methods Primary tumor tissues and plasma and gastric juice of 50 patients with gastric adenocarcinoma were collected.Gastric mucosa tissue,plasma and gastric juice of 20 patients with chronic superficial gastritis and 20 patients with benign gastric ulcer and 30 patients with chronic atrophic gastritis were collected as controls.After sodium-bisulfite treatment,extracted DNA was amplified for DAP kinase promoter hypermethylation by methylation-specific polymerase chain reac- tion.At the same time the gastric juice autofluroscene spectrums(the excitation wavelength was 288 nm,whereas the range of emission wavelength was 300-800 nm)were detected.Results Among the samples from 50 patients,p16 and DAP kinase hypermethylation were detected in 74.4% and 68.1% of tumor tissues,52.0% and 58.0% of plasma,58.6% and 76.0% of gastric juice.No hypermethylation was detected in samples of chronic superficial gastritis and serum of patients with gastric ulcer.Among the patients with gastric ulcer,p16 and DAP kinase hypermethylation were detected in 10.0% and 20.0% of tissues,5.0% and 15.0% of gastric juice.Among the samples from 30 patients with chronic atrophic gastritis,p16 and DAP kinase were detected in 10.0% and 23.3% of tissues,3.3% and 3.3% of sera,3.3% and 20.0% of gastric juice.The intensity of gastric juice autofluroscence spectrums of patients with gastric carcinoma was much higher than those in controls.The sensitivity of p16 and DAP kinase hypermethylation and autofluorescence spectrums together were 95.6% and 97.8% respectively.Con- clusions Promoter hypermethylation of the p16 and DAP kinase gene detected in plasma and gastric juice consists with that in primary tumor tissues of patients with gastric adenocarcinoma.The combination of DNA hypermethylation and autofluorescence spectrum has a good future in diagnosing gastric carcinoma.