Objective To determine if there is an early developmental resistance to seizure-induced hi ppocampal damage. Methods Five daily pentylenetetrazol-indu ced convulsions in immature rats beginning at postnatal day P10,P60 groups.In b oth groups, the latency of seizure, the latency of Ⅳ/Ⅴ grade, the lasting time of seizure and mortality of rats after seizure were used to measure sensitivity of seizure or the resistance to brain damage. Conventional histopathological me thod was utilized to observe morpbological changes and cell counting of dentate granule cells, CA 3,CA 1 and hilar neurnns. Timm histochemical technique was a dopted to study mossy fiber sprou- ting.Results 1.In the both groups(P10,P60),there were significant differences in the latency of seizure (1.07?0.55 vs 8.27?1.48 P

Objective To investigate molecular biological basis associated with inflammatory and immune response in Qi deficency and blood stasis syndrome. Methods Twelve rats were randomly divided into model group and control group. Rat Genome 230 2.0 Array Gene Chip was used for examination of gene expression profiles in rats with Qi deficiency and blood stasis syndrome and normal rats. Differental expression genes associated with inflammatory and immune mechanism were analyzed. Results Compared with the control group, there were different expression genes in model group, the up-regulated were 831 genes, known functional genes 563, and down-regulated 782, known functional genes 544. The expression level increased in 50 genes and decreased in 17 genes associated with inflammatory and immune response. Conclusion Differental expression genes associated with inflammatory and immune response revealed that there was close correlation between Qi deficency and blood stasis syndrome and abnormal inflammatory and immune response.

Objective To observe the therapeutic effects of combination of TCM with western medicine for depressive neurosis.Methods The therapeutic group included 30 cases,patients were treated by combination of TCM with western medicine.The control group included 30 eases,patients were treated only with western medicine.Four weeks was a course of treatment.Results The efficacy of the therapeutic group was 96.7% ,the efficacy of the control group was 66.7% ,there is a significant difference between the two groups(P <0.01).Conclusion The combination of TCM with western medicine for depressive neurosis is effective with short course of treatment.

Objective To assess global and regional cardiac twist function in hypertrophic cardiomyopathy(HCM) patients by velocity vector imaging(VVl).Methods Thirty HCM patients and 33 normal subjects were enrolled into this study.Two dimensional echocardiographic images of parasternal left ventricular basal level,middle and apical level short axis plane,apical four-and two-chamber plane were obtained.Maximal rotation degree,peak rotation rate,circumferential strain (CS) and strain rate (CSR),radial strain(RS) and strain rate(RSR) in systole were measured using VVI offline software.In addition,diastolic peak un-rotation rate,untwisting rate(unTwR) were measured.Each parameters was analyzed both in subendocardial and subepicardial layer.Results There was no significant difference in left ventricular ejection fraction between two groups.EDV,ESV,SV were significantly decreased (P＜0.01) in HCM group.Left ventricular twist(LVtw),CS of subendocardial layer were higher in HCM group than control group(P＜0.05),while the peak rotation rate,un-rotation rate,CS,CSR were lower of subepicardial layer (P＜0.05).The RSR of each short axis level were significantly decreased in HCM group (P＜0.01).The diastolic unTwR was decreased in HCM group (P＜0.05).For further analysis of the two subgroups in HCM,more regional torsion deterioration occurred in patients with left ventricular outflow tract obstruction than those without obstruction.Conclusions The global cardiac twist angle of HCM was higher than normal person,but the circumferential deformation ability of regional myocardium was impaired,left ventricular outflow tract obstruction may aggravate the regional myocardial dysfunction.

Objective To study the exposure dose-effectiveness of ? irradiation on killing the plasmodium in the mice RBC,for the further exploration on the method that could kill the plasmodium in RBC without affecting the activity and function of normal RBC. Methods After infection with Plamodium yoelii (P.y),blood was collected from mice and exposed to ? irradiation (radiated group). An unirradiated group served as control. In the irradiated group,P.y infected blood was divided into three aliquots,each aliquot was irradiated one time by ? radiation using Gammacell 1000 Elite blood radiation apparatus. The dosage of each aliquot was 25,35 and 45Gy. After irradiation,the blood samples were stored at 4℃. Then mice were inoculated with these irradiated blood stored for 1,3 or 5 days after irradiation,or with unirradiated blood. Two days later,the blood samples were taken from inoculated mice and were examined under microscope and plasmodium infection rates were calculated. Results The mice in the control group had parasitemia much earlier than those in irradiated group (1—2 days),and the plasmodium infection rate in the control group was significantly higher than that in the irradiated group(3.7% vs 0.07%). With increasing dosage of irradiation,the survived plasmodium in blood decreased,and survival of mice increased(8—12 days). After 45 Gy irradiation and 5 day storage at 4℃,there were no plasmodium found in the red blood cell of inoculated mice. In the control group,blood testing result was positive,and all the mice died.Conclusion Plasmodium in mice RBC can be killed effectively when blood is exposed to 45Gy irradiation and stored at 4℃ for 5 days.

Objective To explore the immuniated function of infants with bronchiolitis. Methods In vitro production of IL-18,12, 10, 4, IFN-? by peripheral blood mononuclear cells (PBMC) and plasma IgE of bronchiolitis infants measured by enzyme-linked im-munosorbent assay, expression of lymphocyte activation gene - 3 (LAG - 3) mRNA from PBMC measured by semi - quantitative RT- PCR.Forteen healthy children were as normal control. Results Compared with normal control, IL - 12 (390. 94 ? 95. 88 vs 452.44?195.07) ng/L and IL-18(146.23? 55. 81 vs 246.69?121.29) ng/L activity were significantly decreased (P0.05) ;The levels of IL-4(30. 52 ? 17. 65 vs 16.20? 8.94) ng/L and IL-10 (302. 40 ? 100.04 vs 195.30? 84.01) ng/L were elevated significantly(P

Objective To establish a satisfactory method of isolating,culturing and determining gut neural crest stem cells,which may provide theoretical basis for clinical application.Methods The guts of embryonic mice removed and dissociated were plated into serum-free DMEM/F12 medium.The mitogen-free DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs.Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.Results Neurospheres were generated in the simplified serum-free medium.The staining results showed that enteric neurospheres were GNCSCs and could differentiate into neurons,glial cells and smooth muscle cells by serum-induction.Conclusion GNCSCs have the capacity of self-renewal and proliferation,and give rise to neurons,glial cells and myofibroblasts.

0.05).Except that the wet weight of the epididymis in high-dose group of apigenin was significantly different compared with middle-dose group of apigenin(P 0.05).Cell cycle analysis showed that compared with the negative control group,the percent of diploid cells in low and high-dose group was decreased,the proportion of G0/G1 phase cells in low-dose group of apigenin was reduced(P

OBJECTIVE: To simulate the distribution state of lutein molecules in modified starch by dissipative particle dynamics method. METHODS: Materials Studio 4.0 Software was used with Visualizer Module to build 3D models of lutein and modified starch. The Discover and Amorphous Cell modules were used to calculate the solubility parameter. Dissipative particle dynamics module was used to simulate lutein distribution in the microcapsules. SEM was used to investigate the structure of the microcapsules. RESULTS: Dissipative particle dynamics simulations showed the aggregation morphology of modified starch inclusion of lutein molecules and the bead movement ability. Dissipative particle dynamics numerical simulation was verified by in vitro experiments, and the results were satisfactory. The feeding ratio of modified starch and lutein was 100;40. The microcapsules production rate was 47.63%, and the microcapsulation efficiency was 85.79%. The particle size of the microcapsules was 70 μm. CONCLUSION: Dissipative particle Dynamics simulation can show the distribution of drugs in microcapsules very well and can be used to predict the feeding ratio.