1.Analysis on evaluation tool for literature quality in clinical study.
Qing LIU ; Wei ZHAI ; Ya-qin TAN ; Juan HUANG
Chinese Acupuncture & Moxibustion 2014;34(9):919-922
The tools used for the literature quality evaluation are introduced. The common evaluation tools that are publicly and extensively used for the evaluation of clinical trial literature quality in the world are analyzed, including Jadad scale, Consolidated Standards of Reporting Trials (CONSORT) statement and Grades of Recommendations Assessment, Development and Evaluation (GRADE) system and the others. Additionally, the present development, updates and applications of these tools are involved in analysis.
Biomedical Research
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standards
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Evaluation Studies as Topic
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Humans
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Publications
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standards
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Quality Control
2.Expression of cell cycle regulator ATM, Chk2 and p53 and their clinicopathological correlation in breast infiltrative ductal carcinoma.
Xiao-juan PEI ; Qing-xu YANG ; Shao-jie LIU ; Min SU ; Zhuo-ya HUANG ; An-jia HAN
Chinese Journal of Pathology 2012;41(7):479-480
Ataxia Telangiectasia Mutated Proteins
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Breast
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metabolism
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pathology
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Breast Neoplasms
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metabolism
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pathology
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Carcinoma, Ductal, Breast
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metabolism
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pathology
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Carcinoma, Intraductal, Noninfiltrating
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metabolism
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pathology
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Cell Cycle Proteins
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metabolism
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Checkpoint Kinase 2
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DNA-Binding Proteins
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metabolism
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Female
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Humans
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Lymphatic Metastasis
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Neoplasm Grading
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Protein-Serine-Threonine Kinases
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metabolism
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Tumor Burden
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Tumor Suppressor Protein p53
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metabolism
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Tumor Suppressor Proteins
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metabolism
3.Expression of osteopontin and matrix metalloproteinase-9 in human malignant melanoma
Chang-Zheng HUANG ; Jing YANG ; Juan TAO ; Si-Yuan CHEN ; Ya-Ting TU ;
Chinese Journal of Dermatology 1994;0(06):-
Objective To investigate the expression of osteopontin and matrix metalloproteinase-9 (MMP9),and the relationship of osteopontin and MMP9 in malignant melanoma.Methods Expression of osteopontin and MMP9 was measured by immunohistochemical SP method in 23 patients with primary cuta- neous malignant melanoma,17 patients with metastatic melanoma and 20 patients with pigmented nevus. Results Osteopontin and MMP9 were expressed respectively in 87.5% and 75.0% of 40 malignant melanoma specimens,15.0% and 10.0% of 20 pigmented nevus specimens.The expression of both osteo- pontin and MMP9 was significantly higher (both P<0.05) in malignant melanoma than in pigmented ne- vus.There was no correlation between the expression of osteopontin and MMP9,with age,sex,lymph node metastasis or location of lesions (P>0.05).Twenty-nine cases were positive for both osteopontin and MMP9,4 negative for either osteopontin or MMP9.Conclusion Both osteopontin and MMP9 were over- expressed in malignant melanoma,but neither was related to lymph node metastasis.
4.Effects of nuclear factor-?B decoy oligodeoxynucleotide on the function of human umbilical artery smooth muscle cells induced by umbilical sera in preeclampsia
Rong-Zhen JIANG ; Ya-Juan HUANG ; Jing-Hong GU ; Wei-Hong MU ; Han-Ping CHEN ;
Chinese Journal of Obstetrics and Gynecology 2001;0(02):-
Objective To study the effects of nuclear factor-?B(NF-?B)decoy oligodeoxynucleotide(ODN)on the preeclamptic umbilical serum induced expression of precollagen Ⅰ,Ⅲ mRNA and tumor necrosis factor-?(TNF-?)in cultured human umbilical artery smooth muscle cells (HUASMC).Methods Primary cultured HUASMC of normal pregnancy were divided into four groups: group A(HUASMC were incubated with umbilical serum of normal pregnancy);group B(HUASMC were incubated with umbilical serum of preeclampsia);group C(HUASMC were transfected with NF-?B cis decoy ODN 48 h before incubation with umbilical serum of preeclampsia);group D(HUASMC were transfected with NF-?B scramble ODN 24 h before incubation with umbilical serum of preeclampsia).NF-?B cis decoy ODN and NF-?B scramble ODN were transfected with cationic lipofectamine to the latter two groups,respectively.The proliferation of human umbilical artery smooth muscle cells was evaluated by methyl thiazolyl tetrazolium and the apoptosis was analyzed by flow cytometry.The expression levels of precollagen Ⅰ,Ⅲ mRNA were detected by RT-PCR,the expression levels of TNF-? were detected by western blot.Results(1)The proliferation of group B(0.19?0.02)and group D(0.18?0.03)was significantly increased as compared with those of group A(0.11?0.02)and group C(0.14?20.02)(P0.05).(5)The expression of TNF-? of group B(0.74?0.11),group C(0.36?0.09)and group D(0.79?0.12)were significantly higher than that of group A(0.15?0.03)(P0.05).Conclusions NF-?B cis decoy ODN could down-regulate the proliferation,as well as the expression levels of precollagen and TNF-? of HUASMC induced by umbilical serum of preeclampsia.NF-?B may play an important role in the pathogenesis of placental artery abnormalities in preeclampsia.
5.Influence of Longmuzhuanggu Powder on Gastrointestinal Movement and Plasm Motilin in Rats
hui, XU ; yan-jun, LUO ; juan, HUANG ; ya-bin, WU ; lei, YANG
Journal of Applied Clinical Pediatrics 2006;0(19):-
Objective To observe the influence of longmuzhuanggu powder(LMZGP)on gastroenteric movement and plasm motilin(MTL)in rats.Methods Rats were randomly divided into control group,model group,LMZGP large dose group,LMZGP middle dose group,and LMZGP low dose group.To establish the model of disorder of gastric depletion and small intestinal advancement by using maxolon,atropine,neostigmine and adrenaline.Gastric emptying and intestinal progradating rates in mice were tested with black nutritious semi solid cataplasm.Level of MTL in hemtatoplasma was tested by radio immunoassay.Results Maxolon accelerated gastric depletion and neostigmine accelerated intestine propagation and heightened the level of MTL in hematoplasma(P_a
6.Effect of comprehensive intervention on antimicrobial prophylaxis in clean incision surgery
Yinghua ZHANG ; Jinai QIN ; Lanlan LI ; Juan HUANG ; Xiaohong HUANG ; Jingjing YA ; Qunxiu GUO ; Yue QIU ; Xibing WANG ; Daohai CHENG
Chinese Journal of Infection Control 2016;15(2):111-113
Objective To evaluate the effect of comprehensive intervention on perioperative antimicrobial prophy-laxis in clean incision surgery in a hospital.Methods From 2011 ,clean incision surgery cases were performed com-prehensive intervention,antimicrobial use in 2011 -2013 were compared.Results A total of 5 945 cases of clean in-cision surgeries were investigated between 2011 and 2013,3 827 cases (64.37%)received prophylactic use of anti-microbial agents.Prophylactic antimicrobial usage rates in 2011 -2013 were 84.95%,69.99%,and 52.97% re-spectively(χ2 =380.94,P <0.001);the correct rates of medication time were 50.97%,79.99%,and 98.95% re-spectively(χ2 =827.02,P <0.001 );the percentages of prophylactic antimicrobial use ≤24 hours were 24.91 %, 39.96%,and 64.95% respectively(χ2 =422.55,P <0.001 );additional antimicrobial usage rates during surgery were 50.00%,60.00%,and 80.00% respectively(χ2 =59.47,P <0.001 ).Conclusion The implementation of comprehensive intervention measures can standardize antimicrobial use,reduce prophylactic antimicrobial usage rate,improve the correct rate of medication time,shorten the duration of antimicrobial use,and implement addition-al use of antimicrobial agents during surgery.
7.Mixed infection and risk factors in children with severe adenovirus pneumonia.
Fang DU ; Ying HUANG ; Chang SHU ; Li-Juan YIN ; Ya-Na HUANG
Chinese Journal of Contemporary Pediatrics 2013;15(5):375-378
OBJECTIVETo investigate the mixed infection and analyze risk factors in children with severe adenovirus pneumonia.
METHODSA retrospective analysis was performed on the clinical data of 756 children with adenovirus pneumonia between June 2009 and June 2011. Pathogens and risk factors were studied in 216 severe cases.
RESULTSOf the 216 severe cases, 138 (63.9%) were aged from 6 months to 2 years, and 161 (74.5%) developed the disease in the winter and spring; 177 (81.9%) were affected by 1-4 pathogens besides adenovirus, including 74 cases (34.3%) infected with one pathogen as an addition. A total of 334 pathogen strains were identified from the respiratory secretions and sera of the 216 cases. Of them, 163 (48.8%) were bacterial strains, dominated by Gram-negative bacteria (124 strains), 108 (32.3%) were viral strains, and 40 (12.0%) were fungal strains. Multivariate logistic regression analysis indicated that congenital heart disease, congenital airway abnormalities, nutritional anemia, recurrent pulmonary infection, and surgical history were the independent risk factors for severe adenovirus pneumonia in children, with odds ratios of 3.3, 11.1, 7.2, 14.3 and 12.9 respectively (P<0.05).
CONCLUSIONSSevere adenovirus pneumonia is mostly seen in children aged from 6 months to 2 years and occurs frequently in the winter and spring. Many cases are also infected with other pathogens, most commonly Gram-negative bacteria. Congenital heart disease, congenital airway abnormalities, nutritional anemia, recurrent pulmonary infection and surgical history are the independent risk factors for severe adenovirus pneumonia in children.
Adenoviridae Infections ; epidemiology ; etiology ; microbiology ; Child ; Child, Preschool ; Coinfection ; epidemiology ; microbiology ; Female ; Humans ; Infant ; Infant, Newborn ; Logistic Models ; Male ; Pneumonia, Viral ; microbiology ; Retrospective Studies ; Seasons
9.Risk factors for coronary artery lesions secondary to Kawasaki disease in children.
Yong-Chao DENG ; Xun WANG ; Xi-Chun TANG ; Cai-Zhi HUANG ; Juan YANG ; Li-Ya MO
Chinese Journal of Contemporary Pediatrics 2015;17(9):927-931
OBJECTIVETo explore the risk factors for coronary artery lesions (CAL) secondary to Kawasaki disease (KD) in children.
METHODSThe medical data of 895 children with KD were retrospectively reviewed. The patients were classified into two groups according to the presence of CAL: CAL (n=284) and control (n=611). The clinical and laboratory indices were compared between the two groups. The risk factors for the development of CAL in children with KD were identified by multiple logistic regression analysis.
RESULTSMale gender (OR=1.712), occurrence of non-CAL complications (OR=2.028), atypical KD (OR=3.655), intravenous immunoglobulin (IVIG) resistance (OR=2.912), more than 5 days of fever duration before IVIG treatment (OR=1.350), and increased serum procalcitonin (PCT) level (OR=1.068) were the independent risk factors for the development of CAL in children with KD (P<0.05), whereas increased serum albumin (Alb) level was a protective factor (OR=0.931, P<0.05). The areas under the receiver operating characteristic curve of serum PCT and ALB for prediction of the development of CAL in children with KD were 0.631 and 0.558, respectively.
CONCLUSIONSMale gender, atypical KD, occurrence of other non-CAL complications, long duration of fever and IVIG resistance are associated with an increased risk for CAL in children with KD. Serum PCT and ALB have little value in the prediction of CAL in children with KD.
Adolescent ; Calcitonin ; blood ; Calcitonin Gene-Related Peptide ; Child ; Child, Preschool ; Coronary Artery Disease ; etiology ; Female ; Humans ; Immunoglobulins, Intravenous ; therapeutic use ; Infant ; Male ; Mucocutaneous Lymph Node Syndrome ; complications ; Protein Precursors ; blood ; Risk Factors
10.Pharmacokinetics of a fusion protein for human acidic fibroblast growth factor and transcriptional activator protein in rat and its penetration across blood-brain barrier.
Peng-hui YANG ; Hua XU ; Qi-hao ZHANG ; Juan LI ; Yao-ling XIONG ; Ya-dong HUANG ; Zhi-jian SU ; Qing ZHENG
Acta Pharmaceutica Sinica 2011;46(10):1204-1208
This paper is to report the study of the pharmacokinetics of a fusion protein TAT-haFGF(14-154) for human acidic fibroblast growth factor and transcriptional activator protein in rat plasma, and the investigation of their penetration across blood-brain barrier in mice and rats, in order to provide a basis for clinical development and treatment of Alzheimer's disease. Enzyme-linked immunosorbent assay (ELISA) was used to determine concentration of TAT-haFGF(14-154) in rat plasma and in mouse brain homogenate; and immunohistochemistry was used to analyze the distribution in brain. The concentration-time curve fitted two-compartment open model which was linear kinetics elimination after a single intravenous injection of TAT-haFGF(14-154) in rat at the dose of 300 microg x kg(-1). The half life time was 0.049 +/- 0.03 h for distribution phase and 0.55 +/- 0.05 h for elimination phase, and the weight was 1/C2. The result showed that TAT-haFGF(14-154) could be detected in the brain by ELISA and immunohistochemistry, the elimination of TAT-haFGF(14-154) in rat was swift, and TAT-haFGF(14-154) could penetrate across the blood-brain barrier, distribute in pallium and hippocampus and locate in the nucleus.
Animals
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Blood-Brain Barrier
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metabolism
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Brain
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metabolism
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Cell Nucleus
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metabolism
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Cerebral Cortex
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metabolism
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Female
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Fibroblast Growth Factor 1
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administration & dosage
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pharmacokinetics
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Gene Products, tat
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administration & dosage
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pharmacokinetics
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Hippocampus
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metabolism
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Injections, Intravenous
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Male
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Mice
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Rats
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Rats, Sprague-Dawley
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Recombinant Fusion Proteins
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administration & dosage
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pharmacokinetics