1.Primary visceral myopathy: report of a case.
Yu-hua ZHOU ; Ya-bin ZOU ; Miao LI ; Xun SUN
Chinese Journal of Pathology 2012;41(8):568-569
2.Primary cardiac embryonal rhabdomyosarcoma: report of a case.
Liang GUO ; Zhen-yu WANG ; Ya-bin ZOU ; Li-rong BI
Chinese Journal of Pathology 2013;42(9):621-622
Calbindin 2
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metabolism
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Heart Neoplasms
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metabolism
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pathology
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surgery
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Humans
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Male
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Middle Aged
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MyoD Protein
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metabolism
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Myogenin
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metabolism
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Rhabdomyosarcoma, Embryonal
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metabolism
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pathology
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surgery
3.Primary small cell carcinoma of the breast: report of a case.
Li-mei QU ; Gang ZHAO ; Ya-bin ZOU ; Yu-E SONG ; Li-rong BI
Chinese Journal of Pathology 2011;40(2):120-121
Aged
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Breast Neoplasms
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metabolism
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pathology
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surgery
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Cadherins
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metabolism
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Carcinoma, Merkel Cell
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metabolism
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pathology
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Carcinoma, Small Cell
;
metabolism
;
pathology
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surgery
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Diagnosis, Differential
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Female
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Humans
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Lymphoma
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metabolism
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pathology
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Melanoma
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metabolism
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pathology
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Phosphopyruvate Hydratase
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metabolism
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Synaptophysin
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metabolism
4.Primary intracranial mxyoid liposarcoma: report of a case.
Liang GUO ; Dian-bo CAO ; Xu YAN ; Ya-bin ZOU ; Hong-xi MA
Chinese Journal of Pathology 2013;42(12):843-844
5.Comparative effects of Roux-en-Y gastric bypass procedures preserving different gastric volume on blood glucose in Goto-Kakizaki rats.
Zhong-dong ZOU ; Ya-bin JIAO ; Yi-bo WANG ; Chang WANG ; Bin LIU ; Yu WANG ; Sheng HUANG
Chinese Journal of Gastrointestinal Surgery 2012;15(1):39-42
OBJECTIVETo compare the effects of Roux-en-Y gastric bypass (RYGBP)procedures preserving different gastric volume on blood glucose of rats with non-obese type 2 diabetes.
METHODSA total of 36 Goto-Kakizaki rats randomly underwent one of the following procedures: gastric bypass with different types of anastomosis including the Roux-en-Y of total stomach excision(n=12), the Roux-en-Y of partial stomach excision(n=12) and the Roux-en-Y of stomach preservation(n=12). Rats were observed for 24 weeks after surgery. Body weight, food intake and fasting blood glucose level were tested at 0(preoperative), 1, 3, 6, 12, 24 weeks. Hemoglobin A1c(HbA1c) level was measured at 0, 12, 24 weeks and glucose tolerance test (OGTT) was performed in conscious rats before (baseline) and then 30, 60, 120, and 180 minutes. Change of blood glucose over time was depicted. Area under curve(AUC) of glucose tolerance were calculated.
RESULTSCompared with preoperative levels, the weight and food intake of all the rats were significantly decreased at 1 week after surgery(P<0.01). At 3 weeks after operation, the weight and food intake were significantly increased compared with 1 week after operation in the Roux-en-Y of partial stomach excision and the Roux-en-Y of stomach retention(P<0.01). In the Roux-en-Y of total stomach excision, the weight and food intake were significantly lower compared with other two groups(P<0.05). At 24 weeks after operation, the levels of fasting blood glucose were (7.3 ± 1.5), (7.5 ± 2.0) and (8.3 ± 1.3) mmol/L, which were lower than the preoperative levels [(13.2 ± 1.6), (13.6 ± 2.5) and (12.9 ± 2.0) mmol/L, P<0.01] in the three groups. There were no significant differences among the three groups(P>0.05). At 24 weeks after operation, the HbA1c levels were(6.3 ± 1.3)%, (6.4 ± 2.0)% and (7.0 ± 1.3)%, which were lower than the preoperative level[(10.2 ± 2.6)%, (9.6 ± 2.5) and (9.9 ± 2.0)%, P<0.01]. There were no significant differences among the three groups(P>0.05). The trend of the glucose tolerance test and AUC were similar in the three groups after operation.
CONCLUSIONRoux-en-Y gastric bypass in non-obese diabetic rats is effective in terms of glucose control and the efficacy of gastric bypass has no obvious association with the stomach volume.
Animals ; Blood Glucose ; metabolism ; Diabetes Mellitus, Type 2 ; blood ; surgery ; Disease Models, Animal ; Gastric Bypass ; methods ; Male ; Rats
6.Expression of dendritic cell marker CD21 is a positive prognostic factor in diffuse large B-cell lymphoma.
Wei-kai YAO ; Yin-ping WANG ; Fang PENG ; Yan ZHENG ; Ya-bin ZOU ; Jing-na GAO ; Xue-li LIU
Chinese Journal of Pathology 2012;41(12):818-822
OBJECTIVETo analyze CD21 expression in diffuse large B cell lymphoma (DLBCL) and to explore its relationship with the clinicopathological characteristics and prognosis.
METHODSThe clinical data from 80 DLBCL patients who were treated in First Hospital of Jilin University from June 2005 to September 2011 were retrospectively analyzed. The cases were subjected to immunohistochemical staining (SP method) for Ki-67, CD20, CD79a, CD3, CD43, CD5, cyclin D1, bcl-2, CD10, bcl-6, GCET-1, FOXP-1 and MUM-1 protein expression in the tumor tissue. Immunohistochemistry was also used to detect CD21 expression in the tumor tissue. SPSS 18.0 was used to analyze the relationship between CD21 expression and various clinical factors, and the relationship between various clinical factors including CD21 and overall survival.
RESULTSIn the patients aged under 60 years, the incidence of CD21(+) lymphoma (64.0%, 16/25) was significantly higher than that of CD21(-) lymphoma (38.2%, 21/55). There were more CD21(+) lymphoma patients who were at clinical stages I-II (52.0%, 13/25) than patients with CD21(-)lymphomas (23.6%, 13/55). There were also more CD21(+) lymphoma patients (68.0%, 17/25) having less than two extranodal sites involvement than CD21(-)lymphoma patients (41.8%, 23/55). In addition, there were more CD21(+) lymphoma patients with IPI 0-2 (68.0%, 17/25) than CD21(-)lymphoma patients (41.8%, 23/55). There were more CD21(+) lymphoma patients with GCB subtype (60.0%, 15/25) than CD21(-)lymphoma patients (23.6%, 13/55). Death related to DLBCL was less in CD21(+) lymphoma patients (32.0%, 8/25) than CD21(-) lymphoma patients (56.4%, 31/55). Univariate analysis showed that these clinical pathological characteristics affected the overall survival of DLBCL patients, including age, ECOG score, LDH, extranodal involvement, IPI index, CD21 expression, treatment option and efficacy (P < 0.05) . Cox multivariate analysis showed that ECOG score, LDH, extranodal involvement, CD21 expression were closely related to prognosis, and the difference was statistically significant (P < 0.05). Among the 80 patients, the overall survival (OS) of CD21(+) lymphoma patients was significantly higher than that of CD21(-) lymphoma patients.
CONCLUSIONSThe expression of CD21 is associated with young age at onset, early clinical stage, small number of involvement and low IPI index. The OS and median overall survival of CD21(+) lymphoma patients are significantly higher than those of CD21(-) patients. CD21 expression, ECOG score, LDH, extranodal involvement are independent prognostic factors in DLBCL, and in particular, the expression of CD21 is more significant in the prognosis of DLBCL patients.
Adolescent ; Adult ; Age Factors ; Aged ; Aged, 80 and over ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Biomarkers, Tumor ; metabolism ; Child ; Cyclophosphamide ; therapeutic use ; Doxorubicin ; therapeutic use ; Female ; Gastrointestinal Neoplasms ; pathology ; Germinal Center ; pathology ; Humans ; Immunohistochemistry ; L-Lactate Dehydrogenase ; metabolism ; Lymphoma, Large B-Cell, Diffuse ; drug therapy ; metabolism ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Prednisone ; therapeutic use ; Prognosis ; Receptors, Complement 3d ; metabolism ; Retrospective Studies ; Survival Rate ; Vincristine ; therapeutic use ; Young Adult
7.Mutation of MTCYB and MTATP6 is associated with asthenospermia.
Chun-Qiong FENG ; Yan-Bin SONG ; Ya-Guang ZOU ; Xiang-Ming MAO
National Journal of Andrology 2008;14(4):321-323
OBJECTIVETo explore the correlation of the mutation of MTCYB and MTATP6 genes in sperm mitochondria with asthenospermia.
METHODSWe extracted mtDNA from 80 semen samples of asthenospermia and 20 of normal sperm motility, amplified the MTCYB and MTATP6 genes by PCR, and analyzed their mutation by sequencing and BLAST matching.
RESULTSThe deletion of both MTCYB and MTATP6 were detected in 20 of the 80 asthenospermia samples, MTCYB deletion in 16 and MTATP6 deletion in 4, accounting for 20% and 5% respectively. Sequencing and BLAST matching revealed G8887A mutation in the MTATP6 gene in the asthenospermia samples, with a mutation rate of 20%, while no regular mutation was noted in MTCYB. Neither significant deletion nor mutation was observed in any of the 20 samples of normal sperm motility.
CONCLUSIONBoth the deletion and mutation of MTCYB and MTATP6 genes in sperm mitochondria might affect sperm motility in adults.
Adult ; Asthenozoospermia ; genetics ; pathology ; Base Sequence ; Cytochromes b ; genetics ; DNA, Mitochondrial ; genetics ; Humans ; Male ; Mitochondrial Proteins ; genetics ; Mitochondrial Proton-Translocating ATPases ; genetics ; Molecular Sequence Data ; Mutation ; Sequence Homology, Nucleic Acid ; Sperm Count ; Spermatozoa ; metabolism ; pathology
8.Research of the spermatozoal gene expression with gene microarrays.
Xiang-Ming MAO ; Chun-Qiong FENG ; Ya-Guang ZOU ; Rong SHI ; Yan-Bin SONG ; Li JIANG
National Journal of Andrology 2006;12(5):401-407
OBJECTIVETo perform the detection of spermatozoal gene expression in order to accelerate the study of spermatozoal molecular biology.
METHODSTo collect the healthy adults sperm and lymphocytes respectively, and then to extract the total RNAs from them by RNeasy mini kit (QIAGEN) or Trizol reagent. Corresponding cDNAs were produced, digested, ligated, finally labeled with Cy3 (sperm) and CyS (lymphocyte) in the course of RD amplifying reactions. Hybridization with self-made microarrays contained 560 probes was carried out after the labeled cDNAs pured by PCR Product Purification Kit.
RESULTSAmong the 560 probes, 72 genes were up-regulated, 321 genes were down-regulated, the others had no different expression. Furthermore, genes associated with replication, transcription, translation and regulative functions were non-different expression or down-regulated, and those belonged to the spermatogenesis associated, sperm associated antigen were up-regulated, but those involved in the glycolysis were up-regulated, in the oxidative phosphorylation were down-regulated.
CONCLUSIONIt had successfully confirmed that there were a plenty of genes expressed in sperm, furthermore the genes expressed were accorded to spermatozoal functions and characteristics.
Adult ; Down-Regulation ; Gene Expression Profiling ; Gene Expression Regulation ; Humans ; Lymphocytes ; metabolism ; Male ; Middle Aged ; Oligonucleotide Array Sequence Analysis ; Polymerase Chain Reaction ; RNA ; isolation & purification ; Spermatozoa ; metabolism ; Up-Regulation
9.Identification of human testicular embryonal carcinoma proteins by two-dimensional gel electrophoresis and mass spectrometry.
Fei LI ; Ya-guang ZOU ; Qi-zhao ZHOU ; Tie-qiu LI ; Wen-bin GUO ; Xiao-wei JING ; Xiang-ming MAO ; Wan-long TAN ; Shao-bin ZHENG
Journal of Southern Medical University 2009;29(8):1585-1587
OBJECTIVETo separate and identify human testicular embryonal carcinoma proteomics using two-dimensional electrophoresis (2-DE) and mass spectrometry.
METHODSImmobilized pH gradient two-dimensional polyacrylamide gel electrophoresis was used to separate the total proteins of the samples. After silver staining, PDQuest 7.30 image analysis software was applied to analyze the 2-DE images. Three of the proteins highly expressed in human testicular embryonal carcinoma were identified by matrix-assisted laser adsorption/ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS).
RESULTS2-DE effectively screened the differentially expressed proteins in the carcinoma tissues. Three proteins highly expressed in the carcinoma were successfully identified.
CONCLUSIONThe proteins of human testicular embryonal carcinoma can be effectively separated and analyzed using 2-DE and mass spectrometry. Proteomic analysis offers a new means for further study of this carcinoma.
Adult ; Biomarkers, Tumor ; analysis ; metabolism ; Carcinoma, Embryonal ; genetics ; metabolism ; pathology ; Electrophoresis, Gel, Two-Dimensional ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; Mass Spectrometry ; Proteomics ; methods ; Testicular Neoplasms ; genetics ; metabolism ; pathology ; Young Adult
10.Correlation of aging with psychological and organic ED: nocturnal electrobioimpedance volumetric assessment of 83 cases.
Wen-Bin GUO ; Ya-Guang ZOU ; Fei LI ; Qi-Zhao ZHOU ; Tie-Qiun LI ; Xiao-Wei JING ; Shao-Bin ZHENG ; Wan-Long TAN ; Cun-Dong LIU ; Xiang-Ming MAO
National Journal of Andrology 2010;16(10):915-918
OBJECTIVEThe ratio of psychological to organic ED changes with aging. This study aimed to analyze the results of nocturnal electrobioimpedance volumetric assessment (NEVA) for ED patients of different age groups and their significance in the diagnosis of ED.
METHODSA total of 83 ED patients were divided into 4 age groups (< or = 29 yr, 30 -39 yr, 40 -49 yr and > or = 50 yr) and detected for nocturnal penile tumescence (NPT) by NEVA.
RESULTSThirty-four of the cases were diagnosed as organic ED, and the other 49 as psychological ED. With the increase of age, the former was increased from 30.3% in the < or = 29 yr group to 60.0% in the > or = 50 yr group, while the latter decreased from 69.7% to 40.0%.
CONCLUSIONThe percentage of organic ED tends to grow with the increase of age, while that of psychological ED is just the opposite.
Adult ; Aging ; Electric Impedance ; Erectile Dysfunction ; diagnosis ; physiopathology ; Humans ; Male ; Middle Aged ; Penile Erection