1.Research progress in mechanisms of cellular entry of Japanese encephalitis virus.
Ya-Xian ZHOU ; Jian-Qiong ZHANG
Chinese Journal of Virology 2014;30(2):188-192
Japanese encephalitis virus (JEV) is a pathogenic mosquito-borne flavivirus which is responsible for outbreaks of severe viral encephalitis. The cellular entry of JEV is a prerequisite for Japanese encephalitis, so the understanding of its underlying mechanisms will provide more approaches for treating such disease. In recent years, increasing research has been conducted to investigate the mechanisms of cellular entry of JEV, and the results of research on other flavivirus have expanded the research directions for JEV. More methods will be used to suppress JEV infection because of the development of E protein antibodies and the discovery of several inhibitors of the cellular entry process. This review will summarize the recent advances in the mechanisms of JEV cellular entry and membrane fusion.
Animals
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Biomedical Research
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trends
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Encephalitis Virus, Japanese
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genetics
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physiology
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Encephalitis, Japanese
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virology
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Humans
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Virus Internalization
2.Facial basal cell carcinoma: the relationship between clinicopathologic analysis and safety surgical margin
Ya ZHANG ; Minggang WANG ; Hangcheng ZHOU
Chinese Journal of Clinical and Experimental Pathology 2017;33(1):68-72
Purpose To study clinical pathological characteristics,margin status and its influencing factors in different type of facial basal cell carcinoma (BCC).Methods The histopathological features,margin status of the first frozen section and influencing factors was retrospectively analyzed,with review of the relevant literature.The primary outcome variable was the rate of initially positive frozen section margins.Multivariable Logistic regression was used to study histologic subtype,surgical margins,tumor size,location and other factors influence on the rate of initially positive frozen section margins.Results The pathological subtype is the major risk factor,the infiltrative (OR =4.463,95% CI =1.919-10.380,P <0.05) and morpheaform (OR=5.018,95%CI=2.025-16.623,P<0.05) had higher risk on positive surgical margin compared with the nodular.The rate of initially positive frozen section margins of nodular and superficial BCC at different margins were observed but the difference were not significant (P > 0.05).Conclusion The pathological subtype is the major risk factor.Surgical excision with a 3 mm margin can achieve ideal results for nodular and superficial BCC in facial area.
3.The effect of microwave ablation combined with dendritic cell tumor vaccine treatment on mouse mammary carcinoma and its immunological mechanism
Ya ZHOU ; Shehua REN ; Yongju LI ; Ming ZHANG ; Honglian ZHOU
Chinese Journal of Physical Medicine and Rehabilitation 2014;36(2):91-94
Objective To investigate the therapeutic effects of microwave ablation combined with dendritic cell (DC) tumor vaccine on a murine breast cancer model and to explore its immunological mechanism.Methods Forty-eight Balb/c mice were used to establish models of mouse mammary carcinoma,and then randomly divided into a sham-operation group,a microwave ablation treated group,a DC tumor vaccinated group and a microwave ablation plus DC tumor vaccinated group.Microwave ablation was conducted with the microwave ablation group on day 14 after establishing the model.DC tumor vaccination was performed on day 7 in the vaccinated group.In the microwave ablation plus DC tumor vaccinated group,DC tumor vaccination and microwave ablation were consecutively performed on days 7 and 14,respectively.Tumor growth,lung metastasis and survival were observed.The proportion of CD3+ and CD4+ T cells in splenocytes were analyzed by fluorescence Activated Cell Sorter (FACS) on the 14th day after microwave ablation.Serum levels of interferon-γ (IFN-γ) and interleukin-4 (IL-4) were also detected using ELISA.Results The survival time (56.4 ± 4.3 days),tumor size (67.3 ± 6.1 mm2) and lung metastasis index (0.6 ± 0.4) in the microwave ablation plus DC tumor vaccinated group were all significantly lower than the corresponding averages in the microwave ablation and DC tumor vaccinated group.The proportions of splenic CD3 + T cells (27.8 ±10.3 %) and CD4+ T cells (16.7 ± 8.3 %) in the microwave ablation plus DC tumor vaccinated group were significantly larger than in the control group.Average serum levels of IFN-γ (41.8 ± 22.9 pg/ml) in the microwave ablation plus DC tumor vaccinated group were also significantly larger than in the control group,while the average serum level of IL-4 (15.4± 6.4 pg/ml) was significantly lower.Conclusions Microwave ablation and DC tumor vaccination together could have synergistic effects in treating experimental breast cancer,which might be closely related to the enhanced immune response.
4.Transplantation of bone marrow mesenchymal stem cells promotes recovery of renal ischemia-reperfusion injury in rats
Ting ZHANG ; Yun ZHOU ; Ya ZHANG ; Zhihua HONG ; Xiangming YAN
Basic & Clinical Medicine 2006;0(08):-
Objective To investigate the therapy effects of bone marrow mesenchymal stem cells (MSCs) on renal recovery after ischemia-reperfusion injured. Methods Seventy-two Spargue-Dawley rats were randomly divided into 4 groups as normal control, sham operated control, I/R group(n=30) and the MSCs group. Rats were subjected to 45 min bilateral renal ischemial-reperfusion injury with microvascular clips, after 60 min of reperfusion they were injected in BrdU positive MSCs(1?106/mL)intravenously. The animals were sacrificed at 12 h, 3 d, 7 d, 14 d, and 42 d after reperfusion, and the bilateral kidney and blood samples were harvested. The blood urea nitrogen(BUN) and serum creatinine(Scr) were measured on automatic biochemistry analyzer. Renal morphologic changes were scored with Paller’s criterion on hematoxylin and eosin(H&E) stained sections. The apoptosis of tubular cells were examined by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL). PCNA positive tubular cells were detected immunohistochemically as proliferation index. And confocal microscopy were used to identified the distribution of BrdU positive MSCs. Results After 12 h, 3 d of reperfusion, the Scr value of MSCs group were signifcantly lower than I/R group(P
5.Small cell carcinoma of the gastric cardia: a histopathological analysis.
Li-qun GONG ; Ya-zhou AO ; Chuan-gui ZHANG
Chinese Journal of Oncology 2006;28(3):216-217
Adult
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Aged
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Carcinoma, Small Cell
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metabolism
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pathology
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therapy
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Cardia
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Chemotherapy, Adjuvant
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Chromogranin A
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metabolism
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Female
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Follow-Up Studies
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Gastrectomy
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methods
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Humans
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Male
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Middle Aged
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Phosphopyruvate Hydratase
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metabolism
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Stomach Neoplasms
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metabolism
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pathology
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therapy
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Survival Rate
6.Adipose-derived stem cells differentiate into vascular endothelial cells
Lin LIU ; Ya ZHANG ; Yun ZHOU ; Jingmei ZHAI ; Xu CAO
Chinese Journal of Tissue Engineering Research 2013;(23):4224-4231
10.3969/j.issn.2095-4344.2013.23.007
7.Lipid peroxidation injures in hemorrhagic shock at high altitude by oxygenate solution resuscitation in rabbits
Qiquan ZHOU ; Heping WU ; Shi ZHANG ; Ya LAI
Chinese Journal of General Surgery 1997;0(06):-
Objective To investigate the antishock effect of oxygenate solution and its possible mechanism. Methods The protective effects of oxygenates hypertonic hypercolloid solution on lipid peroxidation injures in hemorrhagic shock in rabbits at 4 700 m high altitude spot was observed. Results The oxygenated solution treatment can obviously reduce the malondialdehyde(MDA) and glutathione(GSH) level in plasma and tissue ; and increase the superoxide diamutase(SOD) and glutathione peroxidase(GSH-PX) level in plasma and tissue of the hemorrhagic shock animals. Conclusions Oxygenatea solution treatment can reduce the lipid peroxidation injure, recover the equilibrium of oxidation and antioxidation with shock body in time, and promote the resuscitation of shock.
8.Construction of prokaryotic expression vector of mouse CD25 extracellular domain
Lin XU ; Feng ZHANG ; Ya ZHOU ; Junmin LUO ; Ming QING
Journal of Third Military Medical University 1983;0(03):-
Objective To construct the prokaryotic expression vector of mouse CD25 extracellular domain and to express it in E coli.Methods Total RNA was isolated from splenocytes of Balb/c mice.The CD25 extracellular domain gene was amplified by RT-PCR and cloned into the PET-32a vector.A positive recombinant,PET-32a-CD25e,was identified by enzyme cleaving and sequencing before its expression in E.coli,and transferred into E.coli BL21(DE3) plysS for its expression.After purification with Ni+ resin and renaturation in vitro,a relative molecular mass(Mr) of the interesting protein was detected by SDS-PAGE and Wes-tern blotting.Effect of the purified interesting protein on the proliferation of splenocytes from T cell vaccine-immunized syngeneic mice was detected by MTT assay.Results The cloned CD25 extracellular domain gene was identified to be functional by sequencing and expression.The purified interesting protein could significantly induce the proliferation and IL-4 secretion of splenocytes from T cell vaccine-immunized mice in vitro.Conclusion Mouse CD25 extracellular domain gene can be successfully expressed in prokaryotic cells with biological activity,which lays a foundation for further relative studies.
9.The negative effects of atomization inhalation in mechanical ventilation patients:a retrospective study
Zhongman ZHANG ; Ya WANG ; Chun ZHOU ; Shuang REN ; Yongming TIAN
Chinese Journal of Practical Nursing 2017;33(16):1256-1259
Objective To analyze and discuss the negative effects of atomization inhalation in mechanical ventilation patients in intensive care unit. Methods A retrospective study of all the mechanical ventilation patients in the general ICU of our hospital was carried out from Aprilto December 2015. The risk of multidrug-resistant organisms(MDROs)colonizationin the lower respiratory tract and ventilation associated pneumonia(VAP)in mechanical ventilation patients were analyzed. Results A total of 922 patients were monitored, 160 of whom had atomization inhalation, 92 for MDROs colonizationin the lower respiratory tract and 18 for VAP. The rates ofatomization inhalation in patients with and without MDROs colonizationin the lower respiratory tract were 30.4%(28/92)and 15.9%(132/830)with statistical difference (χ2=12.193, P=0.000). And those in patients with and without VAP were 50.0%(9/18) and 16.7%(151/904), with statistical difference (χ2=11.420, P=0.000). Atomization inhalation was the independent risk factor both of MDROs colonizationin the lower respiratory tract(OR=1.917, 95%CI1.163-3.159, P=0.011) and VAP(OR=4.613, 95%CI 1.773-12.002, P=0.000) in mechanical ventilation patients. Conclusions Atomization inhalation may increase the risk of MDROs colonizationin the lower respiratory tract and VAP in mechanical ventilation patients. Thus unnecessary and too frequent operations of atomization inhalation should be decreased.
10.Research progress of the role of PPARγ in autoimmune diseases
Yan YANG ; Yu ZHOU ; Ya-zi WEI ; Tian-tai ZHANG
Acta Pharmaceutica Sinica 2022;57(10):3124-3132
Autoimmune diseases (AID) are characterized by autoimmune disorder, as autologous tissue is attacked by the autoimmune system. It is reported that the imbalance of autoimmune tolerance and ingrained inflammatory response are the core events of AID undoubtedly. Peroxisome proliferator-activated receptor