1.Clinical Significance of Induced Sputum Clara Cell Secretory Protein and Eosinophil Cationic Protein in Children with Bronchial Asthma
zhi-hong, WEN ; wei-ya, ZHOU ; qiong-yan, HU ; hua, DU ; yuan-feng, LU
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective To explore the roles of clara cell secretory protein(CCSP)and eosinophil cationic protein(ECP)in the pathoge-nesis of bronchial asthma and to evaluate their diagnostic value in asthmatic children.Methods Induced sputum samples were obtained from 31 asthmatic children during chronic persistent period and clinical remission period.According to global initiative for asthma(GINA),the total of 31 cases accepted systemic treatment by inhaling glucocorticoid.The patients included 18 boys and 13 girls aged from 3.7 to 12.0 years,and their average age was 7.6 years.Sputum CCSP concentrations were measured by enzyme-linked immunosorbent assay(ELISA).And the concentrations of sputum ECP were determined with Pharmacia UniCAP system.Results Asthmatic children had significantly lower CCSP levels in sputum during chronic persistent period compared with clinical remission period(P
2.Significance of Detection of Immunoglobulin E and Interleukin-13 in Asthmatic Children
zhi-hong, WEN ; wei-ya, ZHOU ; qiong-yan, HU ; hua, DU ; yuan-feng, LU
Journal of Applied Clinical Pediatrics 2006;0(21):-
Objective To explore roles of total immunoglobulin E(IgE),interleukin-13(IL-13) in asthmatic children,and relation-ship between IgE,IL-13 levels in serum and those in induced sputum.Methods Twenty-six children with asthma who were in chronic persistent period and 20 healthy children were enrolled.Serum and hypertonic saline-induced sputum were obtained in asthmatic children,and serum alone were obtained in control subjects.The levels of IgE were deteced in serum and induced sputum by Pharmacia UniCAP system,and levels of IL-13 were measured by enzyme linked immunosorbent assay(ELISA).Results Asthmatic children had significantly higher serum of IgE and IL-13 levels than those of healthy control group(P0.05).There was positive correlation of IL-13 in serum and induced sputum(r=0.432 P
3.Clinical Signincance of Clara Cell Secretary Protein in Asthmatic Children
zhi-hong, WEN ; sheng-zhou, NONG ; qiong-yan, HU ; wei-ya, ZHOU ; hua, DU ; fang, CHEN ; li-ping, NONG
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To approach the clinical significance of Clara cell secretary protein(CCSP) in bronchial asthmatic children. Methods Serum were collected from 50 cases during asthmatic attacks, 22 asthmatic children who were in stable conditions, and 20 healthy children. Serum CCSP concentrations were measured by a human CCSP enzyme- linked immunosorbent assay (ELISA). Results Asthmatic children had significantly lower levels of CCSP in serum during asthmatic attacks(P
4.Correlative Study on Sera and Induced Sputum Clara Cell Secretory Protein Levels in Children with Asthma
zhi-hong, WEN ; sheng-zhou, NONG ; qing-ling, XIE ; hua, DU ; fang, CHEN ; qiong-yan, HU ; wei-ya, ZHOU
Journal of Applied Clinical Pediatrics 2006;0(15):-
Objective To explore the role of Clara cell secretory protein(CCSP) in asthmatic children and compare the levels of CCSP in sera and induced sputum.Methods Thirty-four children with asthma who were in remission and 25 healthy controls were enrolled.Sera and hypertonic saline-induced sputum were obtained in asthmatic children,and sera alone were obtained in control subjects.The le-(vels) of CCSP were measured in sera and induced sputum by enzyme linked immunosorbent assay.Results Asthmatic children,compared with controls,had significantly lower concentration of CCSP in sera(P
5.Clinical observation on moving cupping therapy combined with moxibustion for treatment of senile habitual constipation.
Zhen-ya JIANG ; Chang-du LI ; Jin-cun LI ; Li GAO ; Qiong-fen WANG
Chinese Acupuncture & Moxibustion 2005;25(12):853-854
OBJECTIVETo explore an effective method for treatment of senile habitual constipation.
METHODSSixty-four cases were randomly divided into a treatment group treated with moving cupping at Hechelu plus Shenque (CV 8) Bazhenxue moxibustion, and a control group with oral administration of Tab phenolphthaleinum, 32 cases in each group. Improvement of constipation in the patients were observed and compared.
RESULTSThe short-term (14 days) clinical cured-markedly effective rate was 84.4% in the treatment group, which was significantly better than 59.4% of the control group (P < 0.05), and there was a significant difference between the two groups in the long-term (2 months) therapeutic effect (P < 0. 01).
CONCLUSIONMoving cupping at the Hechelu combined with Shenque (CV 8) Bazhenxue moxibustion is an effective method for senile habitual constipation.
Acupuncture Points ; Administration, Oral ; Constipation ; Humans ; Moxibustion
6.Clinical study on combination of acupuncture, cupping and medicine for treatment of fibromyalgia syndrome.
Chang-du LI ; Xiao-ying FU ; Zhen-ya JIANG ; Xu-guang YANG ; Si-qin HUANG ; Qiong-fen WANG ; Jia LIU ; Yuan CHEN
Chinese Acupuncture & Moxibustion 2006;26(1):8-10
OBJECTIVETo observe the therapeutic effect of acupuncture at five mental points and moving cupping on the Hechelu of the back on fibromyalgia syndrome (FS).
METHODSSixty-six cases who conformed to the criteria were randomly divided into the treatment group treated with acupuncture at five mental points, moving cupping on the Hechelu of the back and amitriptyline, and the control group treated with amitriptyline. Clinical therapeutic effects were assessed with McGill Pain Questionnaire (MPQ) and HAMD depression scale.
RESULTSThe therapeutic effect of the treatment group was better than that of the control group with a significant difference between the two groups (P < 0.01).
CONCLUSIONCombination of acupuncture with cupping therapy is an effective therapy for fibromyalgia syndrome.
Acupuncture Points ; Acupuncture Therapy ; Biomedical Research ; Fibromyalgia ; Humans ; Pain Measurement
7.The risk surveillance of schistosomiasis in Yunnan, 2021
SHEN Mei-fen ; DU Chun-hong ; SONG Jing ; WANG Li-fang ; SUN Jia-yu ; CHEN Chun-qiong ; FENG Xi-guang ; ZHANG Zhong-ya ; JIANG Hua ; ZHOU Ji-hua ; DONG Yi
China Tropical Medicine 2023;23(2):157-
Abstract: Objective To evaluate the potential transmission risk of schistosomiasis in Yunnan Province, and to provide strategic basis for the prevention and control. Methods Based on the prevalence of schistosomiasis, the social and environmental factors that may lead to the epidemic, 1-3 villages from 3 provincial-level and 15 county-level counties (cities and districts) were selected as the evaluated villages in 2021. The risk of schistosomiasis spread was analyzed comprehensively by consulting, reviewing and collecting routine surveillance data of schistosomiasis in the villages, combined with snail and wild feces survey. The risk level was evaluated for the positive snails, positive wild feces, resident infection, average density of live snails and snail frame occurrence rate. Results Totally 7 snail counties schistosomiasis transmission was blocked of 18 epidemic counties and the rest were eliminated counties. A total of 152 447 snail frames were investigated and 3 043 frames with snails, 15 895 snails were captured and included 15 727 live snails in the 32 evaluated villages. The total area of snail was 58.87 hm2 and the area of reoccurrence was 34.19 hm2 with snail frame occurrence rate of 2.00% and average density of live snails 0.103 2/0.11 m2, and no positive snails were found by loop-mediated isothermal amplification (LAMP) assay. A total of 1 374 wild feces were collected in 27 evaluated villages of 14 epidemic counties, mainly from cattle, dogs, sheep, equine animals, pigs and so on, all of which were negative. According to the risk assessment of epidemic spread, Yongle Village and Yongsheng Village in Eryuan County, Zhiming Village in Chuxiong City were Ⅱ risk, and the rest were Ⅲ risk. Conclusions Although the risk of transmission is low in Yunnan Province, the risk of transmission and spread still exists. It is necessary to strengthen the risk monitoring, control of snail and effective management of livestock to prevent the rebound of the epidemic.
8.Establishment and verification of preeclampsia risk assessment model
Wen-qiong DU ; Feng ZHAO ; Ling-ling GUO ; Jia-xin SHEN ; Ke-ke WANG ; Ying WANG ; Ping ZHANG ; Yong-liang FENG ; Hai-lan YANG ; Su-ping WANG ; Wei-wei WU ; Ya-wei ZHANG
Chinese Journal of Disease Control & Prevention 2019;23(8):981-986
Objective To analyze the risk factors affecting pre-eclampsia, to establish a pre-eclampsia risk assessment model, and to assess the risk of pre-eclampsia early. Methods A face-to-face questionnaire survey was conducted for all women who gave birth in the Department of Obstetrics, the First Hospital of Shanxi Medical University from March 2012 to September 2016. A total of 10 319 qualified questionnaires were collected to exclude 9 623 cases of other hypertensive diseases related to pregnancy. A total of 70% of the subjects were randomly selected as training samples to analyze the influencing factors of pre-eclampsia, and a Logistic regression model was established. The remaining 30% of the objects are used as test samples to verify the effect of the model. Results Logistic regression model was established with training samples. Logit P=-2.517-0.696×Pre-pregnancy lean +0.200 ×Pre-pregnancy overweight +0.944×Pre-pregnancy obesity -1.995×Residential in city -0.409×Folic acid supplemented before pregnancy +1.323×Twin and multiple pregnancy +1.708× History of previous pregnancy hypertension. Homer-Lemeshow test P=0.377. Model AUC=0.767 (95%CI:0.747-0.786, P<0.001). Using the test sample to verify the model, the model sensitivity was 81.68%, the specificity was 75.05%, the positive likelihood ratio was 3.27, and the negative likelihood ratio was 0.24. The test sample model AUC = 0.771 (95%CI=0.763-0.790,P<0.001). Conclusion This study establishes a simple and effective pre-eclampsia risk assessment model with controllable factors. The model has good fit and sensitivity and specificity.
9.Principle and Application of Ultraviolet Crosslinking Immunoprecipitation Technology
Ya-Qiong DU ; Wan-Yao WANG ; Fan GAO ; Yang XU ; Wen-Tao SHI
Progress in Biochemistry and Biophysics 2024;51(1):136-144
The UV cross-linking immunoprecipitation (CLIP) technique was first established in 2003. Sequences of target RNAs and binding sites of specific RNA-binding proteins (RBPs) were identified within the entire transcriptome by UV cross-linking, immunoprecipitation, reverse transcription, and subsequent high-throughput sequencing. Over the last 20 years, CLIP has been continuously modified and improved. Advanced operability and accuracy have extended its application category. Currently, the widely used CLIP technologies include high-throughput sequencing with crosslinking-immunoprecipitation (HITS-CLIP), photoactivatable-ribonucleoside-enhanced CLIP (PAR-CLIP), individual nucleotide resolution CLIP (iCLIP), enhanced CLIP (eCLIP), infrared-CLIP (irCLIP), etc. HITS-CLIP combines high-throughput sequencing with UV cross-linking immunoprecipitation. The 254 nm UV cross-linking and RNAase digestion steps allow the technology to capture transient intracellular RBP-RNA interactions. However, there are limitations in the efficiency of UV cross-linking, with low resolution and high intrinsic background noise. For PAR-CLIP, photoactivatable ribonucleoside was incorporated into RNA molecules, and RBP cross-linked with RNA by 365 nm UV light to improve cross-linking efficiency and resolution. Cross-linking mediated single-base mutations provide more accurate binding site information and reduce interference from background sequences. Long-term alternative nucleotide incorporation, on the other hand, can be cytotoxic and may skew experimental results. iCLIP can identify RBP-RNA cross-linking sites at the single nucleotide level through cDNA circularization and subsequent re-linearization steps, but it has more experimental procedures, and partial cDNAs lost in the circularization step are inevitable. eCLIP discards the radioisotope labeling procedure and reduces RNA loss by ligating adaptors in two separate steps, greatly improving the library-building efficiency, and reducing bias associated with PCR amplification; however, the efficiency of immunoprecipitation cannot be visually assessed at the early stage of the experiment. The irCLIP technique replaces radioisotopes with infrared dyes and greatly reduces the initial number of cells required for the experiment; however, an infrared imaging scanner is essential for the irCLIP application. To address more particular scientific issues, derivative CLIP-related techniques such as PAPERCLIP, cTag-PAPERCLIP, hiCLIP, and tiCLIP have also been developed in recent years. In practice, the aforementioned CLIP approaches have their advantages and disadvantages. When deciding on a technical strategy, we should take into account our experimental objectives and conditions, such as whether we need to precisely define the RNA site for binding to RBP; whether we have the necessary experimental conditions for working with radioisotopes or performing infrared imaging; the amount of initial sample size, and so on. In addition, the CLIP technique has a relatively large number of procedures and can be divided into several successive experimental modules. We can try to combine modules from different mainstream CLIP technologies to meet our experimental requirements, which also gives us more opportunities to improve and refine them and to build more targeted derivative CLIP technologies according to our research objectives.
10.Controlled study on treatment of cervical spondylopathy of the nerve root type with acupuncture, moxibustion and massage as main.
Jian-wei ZHOU ; Zhen-ya JIANG ; Rui-bin YE ; Xian-liang LI ; Xiu-li YUAN ; Fan ZHANG ; Chang-du LI ; Gang LI ; Qi-hua TANG ; Yun-guang HU ; Shuang-chun AI ; Jie CHEN ; Chun-yu LI ; Wei LIAO ; Qiong-fen WANG ; Xiao-bing LUO ; Jing-jing ZHAO ; An-hong LI ; Jie KONG ; Xue-fei QIN ; Song OUYANG ; Jian-ping LUO ; Min WANG ; Guang YANG ; Jin-cun LI ; Fang WANG ; Ying GU ; Li GAO
Chinese Acupuncture & Moxibustion 2006;26(8):537-543
OBJECTIVETo probe into clinical value of comprehensive program of acupuncture, moxibustion and massage as main for treatment of cervical spondylopathy of the nerve root type.
METHODSFive centers, single blind, randomized controlled method were used, 660 cases were divided into a treatment group of 317 cases and a control group of 311 cases. They were treated respectively with comprehensive program of acupuncture, moxibustion and massage as main, and comprehensive program of physical therapy as main. Establish syndrome detection scale and multiply dimensional effect assessment indexes, and evaluate the therapeutic effects and safety.
RESULTSThe cured rate, the cured-markedly effective rate were 42.9%, 64.4% in the treatment group, respectively, better than 16.7%, 36.3% in the control group (P<0.01); after treatment of 2 weeks, clinical symptoms improved in the both groups, but the treatment group was better than the control group in the improvement degrees of neck-shoulder-limb pain, neck rigidity, abnormality of cervical anteflexion, etc. (P<0.01 or P<0.05); the treatment group was shorter than the control group in the time of producing the effect and therapeutic course (P<0.01).
CONCLUSIONComprehensive program of acupuncture, moxibustion and massage as main is safe and effective for treatment of cervical spondylopathy, with a better therapeutic effect compared with the comprehensive program of physical therapy.
Acupuncture Therapy ; Humans ; Massage ; Moxibustion ; Single-Blind Method ; Spinal Diseases