Animals ; Blood Glucose ; drug effects ; Body Weight ; drug effects ; Chronic Disease ; Female ; Hypoxia ; physiopathology ; KATP Channels ; drug effects ; Male ; Propylamines ; pharmacology ; Protective Agents ; pharmacology ; Rats ; Rats, Sprague-Dawley

RNA has received more attention in the field of forensic medicine and the development of the new biological markers based on RNA shows great significance in the analysis of complex cases. circular RNA (circRNA) is a kind of non-coding RNA which is widely reported recently. Although the regulatory mechanisms of generation and expression are not fully clear, the existing research indicates that circRNA has important biological functions. CircRNA has a cell-type-specific expression with great stability and a high expression level, which makes it meaningful in forensic applications potentially. In this paper, the research progress, the generation and regulation of circRNA as well as its biological characteristics and functions are summarized, which will provide references for related studies and forensic applications.
Forensic Sciences ; Humans ; RNA ; RNA, Circular

Objective To establish the method and cardiovascular imaging in mice using ultrasound biomicroscopy(UBM) with anatomical confirmation by magnetic resonance (MR) imaging, and to evaluate the feasibility and value of UBM in mice cardiovascular imaging.Methods The mouse thoracic anatomy was elucidated using Signo 3.0T MR imaging on 2 healthy C57BL/6 adult male mice.The structures and flow of mice heart and great vessels in 16 healthy C57BL/6 adult male mice were showed and analyzed by Visualsonics Vevo 770 UBM through several acoustic windows, including left parasternal, right parasternal, apical regions, and suprasternalis.The systolic and diastolic function of mice heart were also evaluated.ResultsAnatomic characteristic of mice could be displayed by MR imaging.The heart was mostly located in left thoracic cavity.The angle between long axis of the heart and the midline of the chest was about 45 degrees.Left atrium,left ventricle, mitral valve and right ventricle were located on the left side of the midline of the chest.Aortic valve orifice, ascending aorta,right atrium and tricuspid valve were located on the right side of the midline of the chest.Left heart long axis view,left ventricular short axis view,ascending aorta long axis view,long axis view of aortic arch, aortic short axis view, right ventricular inflow tract long axis view, pulmonary artery long axis view and so on were obtained clearly using UBM.The structures of heart and great vessels were displayed and the cardiovascular parameters could be measured accurately,including left atrium,left ventricle,aorta and aortic arch,interventricular septum,right atrium, right ventricle, pulmonary artery, innominate artery, right internal carotid artery and the velocity in each valve orifices from Doppler flow spectrum.No significant difference was found in the measurements between different age groups.Conclusions The cardiovascular structures and hemodynamics could be displayed by UBM in mice when appropriate acoustic windows and sections were selected.This novel imaging protocol provides us a non-invasive way to follow atherosclerosis in genes knockout mice.

The study was aimed to investigate the expression of VEGF mRNA and VEGF protein in HL-60 cells treated with diallyl disulfide (DADS), and to explore the antileukemic mechanism of DADS in respect of VEGF production. Semi-quantitative RT-PCR and ELISA were used to detect the expression of VEGF mRNA and secretion of VEGF protein in HL-60 cell lines treated by DADS respectively. The results showed that the expression of VEGF mRNA and secretion of VEGF protein were found in HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein in HL-60 cells could be down regulated by treatment with 0.625, 1.25, and 2.5 microg/mL DADS for 48 and 72 hours and the effects had a dose dependent relationship (r > 0.9, P < 0.01). The differences between DADS treated HL-60 cell groups and the control group were statistically significant (P < 0.01), there were also statistically significant differences among three DADS-treated HL-60 cell groups (P < 0.05). It is concluded that DADS effectively inhibits the proliferation of human leukemia cell line HL-60 cells; DADS exerts its antileukemic effects by reduction of the expression of VEGF mRNA and VEGF protein secretion.
Allyl Compounds ; pharmacology ; Antineoplastic Agents ; pharmacology ; Cell Proliferation ; drug effects ; Disulfides ; pharmacology ; HL-60 Cells ; Humans ; RNA, Messenger ; biosynthesis ; genetics ; Vascular Endothelial Growth Factor A ; biosynthesis ; genetics

Objective To explore the clinical value of 99Tcm-MIBI G-MPI in patients diagnosed with myocardial bridging(MB) by CTA. Methods Forty-five patients with MB and 17 normal controls diagnosed by CTA(64 slices CT) were included. All patients underwent rest 99Tcm-MIBI G-MPI and 17 MB patients and 9 normal controls also underwent stress 99Tcm-MIBI G-MPI. Myocardial ischemia, function and wall motion were assessed. G-MPI results were compared with CTA results by χ2 test, Fisher exact test and t test. Results In patients with MB, the positive rate of abnormal perfusion by gated stress 99Tcm-MIBI G-MPI was 64.7% (11/17) and 41.2% (7/17) using quantitative analysis and visual evaluation respectively; while the data were 42.2% (19/45) and 22.2% (10/45) by rest G-MPI (P=0.035). The positive rate by rest G-MPI in MB patients was significant different among mural coronary arteries of different depths and different locations. By quantitative analysis of the stress G-MPI, the reversible, fixed, and mixed ischemia patients were 4 (35.3%), 6 (23.5%) and 1 (5.9%) respectively; the reversed, reversible and fixed abnormity of wall motion was found in 4 (23.5%), 4 (23.5%) and 2 (11.8%) patients respectively; the reversed, reversible and fixed wall thickening were found in 6 (35.3%), 5 (29.4%) and 1 (5.9%) patients respectively. There was no significant difference in left ventricular ejection fraction and peak filling rate between MB patients and normal controls in both rest and stress studies (t: from -0.564 to 1.292, all P>0.05). Conclusion The G-MPI may be useful for the evaluation of myocardial ischemia and myocardial function simultaneously in patients with MB.

Objective To observe the effect of Kai Hou Jian-spray in treating herpetic stomatitis of children.Methods A total of 180 children who suffered herpetic stomatitis were randomly divided into treatment group and control group, 90 cases in each group.Patients in control group were given routine treatment scheme such as oral care and water-electrolyte balance,and on the basis of control group, those in treatment group were extra given Kai Hou Jian-spray every 3 to 4 hours and 6 to 7 spray each time.The treatment lasted for 5 to 7 days.And the clinical effects (effective, markedly effective, invalid and total effective rate), signs of symptoms ( the recovery time of body temperature, the disappea-rance time of oral herpes and the hospitalization time ) and adverse reac-tions were observed and compared.Results Compared with control group, the disappearing time of clinical symptoms and the hospitalization time were significantly decreased in a shorter time in treatment group (P<0.05).The total effective rate in treatment group was 95.55%, significantly higher than 77.78% in the control group ( P <0.01 ) . Conclusion Kai Hou Jian -spray combined with drug treatment has significant effect on herpetic stomatitis in children , with a shorter hospitalization time and disappearance time of oral herpes.

Objective To study the protection of total Hedysaripolysaccharide in lipopolysaccharide (LPS) induced acute lung injury (ALI) of mice.Methods The mice were randomly divided into 6 groups:normal group,model group (8 mg · kg-1 lipopolysaccharide),control group (dexamethasone 3 mg· kg-1),and experimental groups in low,middle and high dose (50,100,200 mg · kg-1 total Hedysaripolysaccharide).Tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10)levels in serum were measured with enzyme-linked immunosorbent assay.Total antioxidant capacity (T-AOC),the activities of super oxide dismutase (SOD) and malondialdehyde (MDA) contents in serum were detected by spectrophotometer.And DNA damage of peripheral blood lymphocytes was observed by single cell gel electrophoresis.Results The serum TNF-α and IL-10 levels in model group were (156.23 ± 7.39),(44.24 ± 2.89) ng · L-1,which in middle and high dose of experimental groups were (139.45 ± 6.57),(35.30 ± 1.88);(120.13 ± 6.81),(33.11 ±2.71) ng · L-1,the difference was significant(all P ＜0.01).T-AOC,SOD activities in middle and high dose of experimental groups were (5.91 ± 0.10),(70.00 ± 3.04);(6.10 ± 0.09),(76.21 ± 4.03) U · mg-1,which were obviously higher than those of model group which were (5.56 ± 0.13),(67.68 ± 4.86) U · mg-1 (all P ＜0.01).MDA contents of middle and high dose experimental groups were (5.01 ± 0.11),(4.17 ± 0.12) nmol · mg-1,which were obviously lower than that of model group with(5.55 ±0.11) nmol · mg-1 (all P ＜0.01).DNA tail length (TL) were (5.81 ±0.10),(5.24 ±0.10) μm,tail moment (TM)were (2.86 ±0.12),(2.71 ±0.16) μm,and Olive tail moment(OTM) were (2.67 ±0.10),(2.17 ±0.18) μm in middle and high dose of experimental groups,which were significantly lower than those of model group:TL was (6.45 ± 0.08) μm,TM was (3.22 ± 0.14) μm,and OTM was (3.35 ± 0.12) μm,the differences were all significant (all P ＜ 0.01).Conclusion Total Hedysaripolysaccharide had a protective effect against the lipopolysaccharide-induced acute lung injury.The possible mechanisms may be due to the regulation the proportional imbalance of TNF-α and IL-10,as well as the balance oxidative and anti-oxidative.

Objective To explore the effect of the blog of central venous catheter thoracostomy on the health education and academic exchanges.Methods Many methods were used such as established hosted rental blog at the NetEase Web sites,managed by special administrator,and published specific nursing log on thoracostomy.Results Totals of 33 logs were published by the administrator from October 2011to January 2012.The browse rate of these logs was high and the total visit had exceed 10 000 person-times.Callers had published over 60 comments,answered inquiry on line over 40 times and replied over 10 emails.Conclusions It is an economic,convenient and shortcut method to develop health education and academic exchanges by means of the blog on nursing care of thoracostomy with strong sharing and interaction.This method can meet the requirrnents of the people with different levels of the knowledge on thoracostomy including patients families,health care workers and so on.

OBJECTIVETo study the relationships among pathological and immunohistochemical changes in liver tissues, and the HBeAg, HBV DNA, ALT level in the patients with chronic hepatitis B.

METHODSPathological and immunohistochemical examinations of liver tissue liver function tests, serum HBV and HBV DNA detection were performed in 194 patients with chronic hepatitis B.

RESULTSThere was significant difference between the serum HBeAg positive group and the negative group in G2, G3-4 S2, S3-4 in liver tissues; The serum HBV DNA level of the groups S0 and S1-4, and the hepatic activity index between the groups G0-1 and G2-4 were significantly different. And the hepatic HBcAg positive group and HBcAg negative group were significantly different too. There was no significant difference between the HBsAg level in liver tissues as "+" group and the "++ - +++" group. The pathological diagnosis as S1 or (and) G2 is respectively 28.57%, 53.33%, 80.15%, 77.88% among the four groups with normal-mild-moderate-severe elevated serum ALT level.

CONCLUSIONSerum HBV DNA correlated with HBcAg expression in liver tissue; the HBsAg level in liver tissues have no relationship with the serum HBV DNA level. The patients with low serum HBV DNA level may have high index of hepatic activity and hepatic fibrosis. Asymptomatic carriers and patients with low serum ALT level should be encouraged to accept liver biopsy. It can determine the degree of liver inflammation and fibrosis and timing of treatment.

Adolescent ; Adult ; Alanine Transaminase ; blood ; DNA, Viral ; blood ; Female ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; genetics ; immunology ; physiology ; Hepatitis B, Chronic ; blood ; pathology ; virology ; Host-Pathogen Interactions ; Humans ; Liver ; pathology ; virology ; Male ; Middle Aged ; Young Adult

OBJECTIVETo define the differences in the risk factors for clinical benign prostatic hyperplasia (BPH) between the Mongolian and the Han people.

METHODSBetween January 2003 and June 2005, 63 Mongolian and 63 Han patients with BPH were interviewed using a questionnaire consisting of the risk factors for BPH.

RESULTSThe intake of alcohol and dairy products was higher in the Mongolians than in the Hans (P <0.05). The consumption of milk tea, dairy products and meat was significantly greater in the Mongolians ( >250 g per day) than in the Hans (P <0.01). And there were more smokers (the smoking index >300) with moderate to severe symptoms (IPSS > 7) in the Mongolians than in the Hans (85.71% vs 57. 14%, P <0.01). The incidence of intraprostatic chronic inflammation and calcification within the prostate gland was higher in the Mongolians (28% vs 11% , P < 0.05; 36.5% vs 15.87% , P < 0.01, respectively). No significant difference was observed between the two groups in body mass, blood pressure, marriage age, offspring number, physical activity, IPSS score and PSA level.

CONCLUSIONCompared with Han BPH patients, the Mongolian BPH patients had greater alcohol intake, higher protein diet and higher incidence of intraprostatic chronic inflammation, and those with the smoking index greater than 300 were more likely to receive surgical treatment.

Aged ; Alcohol Drinking ; epidemiology ; Asian Continental Ancestry Group ; China ; epidemiology ; Feeding Behavior ; Humans ; Male ; Middle Aged ; Prostatic Hyperplasia ; epidemiology ; Risk Factors ; Smoking ; epidemiology ; Surveys and Questionnaires