1.Immunohistochemical study on the expression of caspase, bax, bcl-2 and c-kit after SCI in Bufo bufogargarizan.
Ping LI ; Yu ZHANG ; Ya-Fei CAI ; Yan WANG
Chinese Journal of Applied Physiology 2011;27(4):399-401
Animals
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Bufo bufo
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Caspase 3
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genetics
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metabolism
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Female
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Immunohistochemistry
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Male
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Proto-Oncogene Proteins c-bcl-2
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genetics
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metabolism
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Proto-Oncogene Proteins c-kit
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genetics
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metabolism
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Spinal Cord
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metabolism
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Spinal Cord Injuries
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metabolism
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bcl-2-Associated X Protein
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genetics
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metabolism
2.Leiomyosarcoma of mediastinum: report of a cases.
Chang-ping LI ; Ya-ru WANG ; Jun-cai ZHOU
Chinese Journal of Pathology 2007;36(1):58-58
Actins
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metabolism
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Adult
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Desmin
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metabolism
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Diagnosis, Differential
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Histiocytoma, Malignant Fibrous
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pathology
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Humans
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Immunohistochemistry
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Leiomyoma
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pathology
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Leiomyosarcoma
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metabolism
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pathology
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surgery
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Male
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Mediastinal Neoplasms
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metabolism
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pathology
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surgery
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Vimentin
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metabolism
4.Changes of body weight, blood glucose in chronic intermittent hypoxic rats and protection of iptakalim.
Hong SHEN ; Wei-ping XIE ; Hong WANG ; Ya-qin ZHAI ; Jian-kang CAI
Chinese Journal of Applied Physiology 2010;26(2):215-248
Animals
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Blood Glucose
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drug effects
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Body Weight
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drug effects
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Chronic Disease
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Female
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Hypoxia
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physiopathology
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KATP Channels
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drug effects
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Male
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Propylamines
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pharmacology
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Protective Agents
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pharmacology
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Rats
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Rats, Sprague-Dawley
5.Analysis of malaria cases re-examination results of malaria diagnostic reference laboratory in Nantong, Jiangsu
CAO Cai-qun ; DING Gui-sheng ; LU Jin ; GU Ya-ping
China Tropical Medicine 2022;22(09):870-
Abstract: Objective To analyze the laboratory microscopic re-examination results of malaria cases in Nantong of the National Notifiable Disease Report System from 2014 to 2021 by Nantong Malaria Diagnostic Reference Laboratory, so as to evaluate the malaria diagnosis ability of Nantong Malaria Diagnostic Reference Laboratory. Methods The blood smear and blood samples of malaria cases in Nantong from 2014 to 2021 of the National Notifiable Disease Report System were collected. Nantong Malaria Diagnostic Reference Laboratory and Jiangsu Institute of Parasitic Diseases carried out the re-examination of municipal and provincial laboratories, taking the results of provincial laboratory as the standard to compare and analyze the re-examination results of Nantong Malaria Diagnostic Reference Laboratory. Results From 2014 to 2021, the two-level laboratories in Nantong city and Jiangsu Province re-examined the blood samples of 297 malaria cases. The microscopic examination and PCR re-examination results at the provincial level were the same:292 positive cases and 5 negative cases. The qualitative coincidence rate between Nantong microscopic re-examination results and the provincial re-examination results was 100% (297/297), without misjudgment and omission. The coincidence rate of Plasmodium typing was 96.23% (281/292). The coincidence rate of P. falciparum, P. vivax, P. ovale and P. malaria were 99.57% (234/235), 62.50% (5/8), 89.47% (34/38) and 72.73% (8/11) respectively. The consistency test results showed that the Kappa value of Plasmodium typing results between municipal and provincial laboratories was 0.89. The Kappa values of P. falciparum, P. vivax, P. ovale and P. malaria were 0.98, 0.58, 0.87 and 0.79 respectively. Conclusion The malaria diagnosis ability of Nantong Malaria Diagnostic Reference Laboratory is generally good, and it is necessary to improve the ability of Plasmodium typing.
6.Preparation and quality control of soybean isoflavone dropping pills.
Cai-Bin JIANG ; Ya-Ping LIN ; Li-Mei YU
China Journal of Chinese Materia Medica 2007;32(10):906-909
OBJECTIVETo establish the optimized preparation procedure and study the method to determine the content for soybean isoflavone(SIF) Dropping Pills.
METHODThe preparation conditions, such as the proportion between SIF and PEGs, the temperature of mixture of SIF and PEGs, dropping distance, etc., were studied with Uniform Design and One-way ANOVA. SIF was identified by TLC and the content of SIF was determined by UV spectrometry at 262 nm detection wavelength.
RESULTThree batches of the prepared products meet the standards of the Chinese pharmacopoeia on dropping pills. SIF can be identified by TLC. Using UV spectrometry, the linear range of SIF was 0. 407 2 to 4. 072 g x mL(-1) and the correlation coefficient was 0. 999 8. In high, middle and low concentration, average recovery were 96. 54%, 97.27% and 97.21%, respectively (RSD were 1.3%, 0.78% and 0.71%).
CONCLUSIONThe preparation procedure is feasible, simple and suitable, the method established in this paper can be adopted for the quality control of SIF dropping pills, and the determination method is simple, relatively fast and accurate.
Chromatography, Thin Layer ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Isoflavones ; analysis ; isolation & purification ; standards ; Particle Size ; Plants, Medicinal ; chemistry ; Polyethylene Glycols ; chemistry ; Quality Control ; Soybeans ; chemistry ; Spectrophotometry, Ultraviolet
7.Interleukin-8 inhibits clonal expansion of 3T3-L1 preadipocyte during differentiation.
Hua ZHOU ; Xi YANG ; Ya-Ou ZHANG ; Guo-Ping CAI
Chinese Journal of Applied Physiology 2008;24(2):243-247
AIMTo investigate the effect of interleukin-8 (IL-8) on the differentiation and clonal expansion of 3T3-L1 preadipocyte during the differentiation period.
METHODSThe morphological changes of 3T3-L1 cells during differentiation after the treatment of IL-8 was observed by Oil-Red O staining. Glycerol-3-phosphate dehydrogenase (GPDH) activity was measured by a spectrophotometric method. MTT method and 3H-TdR incorporation were applied to examine the changes of cell proliferation and DNA synthesis in clonal expansion of 3T3-L1 cells. Cell cycle analysis was taken by flow cytometry.
RESULTSIL-8 could inhibit the differentiation and GDPH activity in a dose dependent manner. IL-8 decreased the cell proliferation and DNA synthesis in clonal expansion after induction. Also, the proportion of cells in G1 phase was increased and that of cells in S and G2 phase was declined after the treatment of IL-8.
CONCLUSIONIL-8 inhibits the differentiation of 3T3-L1 preadipocytes by decreasing the clonal expansion of the cells.
3T3-L1 Cells ; Adipocytes ; cytology ; metabolism ; Animals ; Cell Cycle ; Cell Differentiation ; Cell Proliferation ; drug effects ; Interleukin-8 ; pharmacology ; Mice
8.Effect of Telbivudine Tablet Combined Jianpi Bushen Recipe on HBV Specific Cytotoxic T Lymphocyte and HBeAg Seroconversion in Patients with HBeAg Positive Chronic Hepatitis B.
Zhong HUA ; Wei XU ; De-cai FU ; Yi-guang LI ; Xiao-ye GUO ; Kang-wan TU ; Ya-ping DAI
Chinese Journal of Integrated Traditional and Western Medicine 2016;36(5):530-534
OBJECTIVETo explore the effect of Telbivudine (LDT) Tablet combined with Jianpi Bushen Recipe (JBR) on serum hepatitis B virus (HBV) specific cytotoxic T lymphocyte (CTL) and HBeAg seroconversion in chronic hepatitis B (CHB) patients.
METHODSTotally 90 HBeAg-positive and human leukocyte antigen (HLA)-A2 positive CHB patients were randomly assigned to the treatment group and the control group, 45 cases in each group. Patients in the treatment group took LDT Tablet (600 mg, once per day) combined with JBR granule (twice per day), while those in the control group took LDT Tablet alone. The therapeutic course for all was one year. HBV DNA negative conversion rate, HBeAg seroconversion rate, and level of HBV specific CTL were compared after 1 year treatment; liver function, drug resistance mutations, and adverse reactions were also compared between the two groups.
RESULTSAfter 1 year treatment, HBV DNA negative conversion rate and HBeAg seroconversion rate were 88.89% (40/45) and 40.00% (18/45) in the treatment group, higher than those of the control group [68.89% (31/45) and 20.00% (9/45)], with statistical difference (P < 0.05). Level of HBV specific CTL in the treatment group was 0.78% +/- 0.09% after treatment, higher than that of the control group after 1 year treatment (0.54% +/- 0.11%) and that before treatment (0.36% +/- 0.07%), with statistical difference (P < 0.01). Level of HBV specific CTL in 27 patients with HBeAg seroconversion was 0.81% 0.10%, higher than that of 63 patients without HBeAg seroconversion (0.60% +/- 0.09%), with statistical difference (P < 0.01). ALT returned to normal in 44 cases of the treatment group (97.78%), while it was 42 cases (93.33%) of the control group, with no statistical difference between the two groups (P > 0.05). Total bilirubin (TBil) in the two groups all turned to normal. rtM204I variation occurred in 1 case (2.22%) of the treatment group and 2 cases (4.44%) in the control group. No obvious adverse reaction occurred in the two groups.
CONCLUSIONLDT Tablet combined with JBR could elevate levels of HBV specific CTL and HBeAg seroconversion in CHB patients.
Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; Hepatitis B, Chronic ; drug therapy ; Humans ; Seroconversion ; T-Lymphocytes, Cytotoxic ; immunology ; Tablets ; Thymidine ; analogs & derivatives ; therapeutic use
9.Comparison of three paeonol gels in their percutaneous penetration performance
Ya-Qin CAI ; Jun FENG ; Yan-Hui HOU ; Wen-Ping WANG
Chinese Traditional Patent Medicine 2018;40(1):66-70
AIM To compare the percutaneous penetration performance of three paeonol gels.METHODS Franz diffusion cell method was applied to investigating the penetration and retention behaviors of eutectic mixturebased nanoemulsion,ordinary nanoemulsion and saturated solution gels onto mouse skins in vitro.The retention and permeation amounts in stratum corneum and hair follicles of volunteers smeared with gels were compared by tape stripping method.RESULTS The accumulative permeation and retention amounts of various gels onto mouse skins in vitro were in sequence of eutectic mixture-based nanoemulsion gel > ordinary nanoemulsion gel > saturated solution gel.The main retention of all the three gels was observed on the volunteers' skin surface,and the permeation amounts of eutectic mixture-based nanoemulsion,ordinary nanoemulsion gels,and their accumulative permeation amounts in stratum corneum and hair follicles were significantly higher than those of saturated solution gel (P <0.05).CONCLUSION Nanoemulsion technology can significantly promote the percutaneous penetration performance of paeonol.
10.Application effect of Fetion in healthy intervention of hemodialysis patients with hypertension
Cai-Ling CHEN ; Ya-Ping WANG ; Hui-Jie WANG ; Ping ZHU ; Jin-Huan WANG ; Yu HE
Chinese Journal of Modern Nursing 2012;18(30):3639-3642
Objective To study the application effect of Fetion in healthy intervention of hemodialysis patients with hypertension.Methods The patients were randomly divided into the intervention group and the control group.Fetion group was established in the intervention group,and nurses send knowledge regarding high blood pressure,noticeable problems of diet and life habits as well as healthy behaviors every week,while the control group received conventional guiding.The intervention time lasted from 6 months to 2 years,with the average of 1.21 year.Two groups' knowledge of high blood pressure,change of habits,reduction of pressure and change of body mass were compared after intervention.Results The rate of awareness of high blood pressure knowledge in the intervention group was significantly higher after the intervention than before (P <0.05).Their life habits were also obviously improved than before (P < 0.05).In the interception group,the systolic pressure (135.67 ± 13.13)mm Hg and diastolic blood pressure (89.12 ± 9.06)mm Hg were significantly lower after the intervention than before (t = 4.632,3.978,respectively; P < 0.05).After intervention,the rate of awareness in intervention group was higher than that that in the control group (P <0.05) ; the life habit in intervention group was better than that in the control group (P < 0.05) ; the systolic and diastolic blood pressure were more obviously lower in the intervention group than those in the control group (P <0.05).Conclusions It is effective to apply Fetion in the healthy intervention hemodialysis patients with hypertension,which is worth popularizing.