3.The effect of cold air and dust weather on the content of IL-6,8- iso-PGF2α and 11-DH-TXB2 in urine.
Ya-xiong WAN ; Bin LUO ; Yan-rong SHI ; Mei-chi CHEN ; Li-na WANG ; Ren-hong WANG ; Jing-ping NIU
Chinese Journal of Applied Physiology 2016;32(1):5-12
Cold Temperature
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Dinoprost
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analogs & derivatives
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urine
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Dust
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Humans
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Interleukin-6
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urine
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Thromboxane B2
;
analogs & derivatives
;
urine
;
Weather
4.Effects of Culture Mechanism of Cinnamomum kanehirae and C. camphora on the Expression of Genes Related to Terpene Biosynthesis in Antrodia cinnamomea
Zhang ZHANG ; Yi WANG ; Xiao-Long YUAN ; Ya-Na LUO ; Ma-Niya LUO ; Yuan ZHENG
Mycobiology 2022;50(2):121-131
The rare edible and medicinal fungus Antrodia cinnamomea has a substantial potential for development. In this study, Illumina HiSeq 2000 was used to sequence its transcriptome.The results were assembled de novo, and 66,589 unigenes with an N50 of 4413 bp were obtained. Compared with public databases, 6,061, 3,257, and 2,807 unigenes were annotated to the Non-Redundant, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases, respectively. The genes related to terpene biosynthesis in the mycelia of A. cinnamomea were analyzed, and acetyl CoA synthase (ACS2 and ACS4), hydroxymethylglutaryl CoA reductase (HMGR), farnesyl transferase (FTase), and squalene synthase (SQS) were found to be upregulated in XZJ (twig of C. camphora) and NZJ (twig of C. kanehirae). Moreover, ACS5 and 2,3-oxidized squalene cyclase ( OCS) were highly expressed in NZJ, while heme IX farnesyl transferase (IX-FIT) and ACS3 were significantly expressed in XZJ. The differential expression of ACS1, ACS2, HMGR, IX-FIT, SQS, and OCS was confirmed by real-time quantitative reverse transcription PCR. This study provides a new concept for the additional exploration of the molecular regulatory mechanism of terpenoid biosynthesis and data for the biotechnology of terpenoid production.
5.Epidemiological status and risk factors of hyperuricemia in rural area of the Three Gorges.
Ya-li QU ; Ling YU ; Zu-ming LI ; Li-hua KONG ; Tong-chu XIAO ; Mei LI ; Ni-na LUO ; Xiong-feng DENG ; Ke-yi QU
Chinese Journal of Cardiology 2013;41(5):427-431
OBJECTIVETo explore the epidemiological status and risk factors of hyperuricemia in rural area of the Three Gorges.
METHODSA cross-sectional survey was carried out in rural area of Yiling District, Yichang City, which was located north-west bank of Xiling Gorge in 2007. A standard structure questionnaire was used to collect demographic data, social-economic status and life-style features. Fasting venous blood was collected and serum uric acid (SUA) was determined. Hyperuricemia was defined as SUA levels ≥ 417 µ mol/L (70 mg/L) in men and ≥ 357 µmol/L (60 mg/L) in women. Multiple logistic regression analysis was used to analysed the risk factors of hyperuricemia.
RESULTSA total of 9354 participants aged 35 and above were included, 19.9% (1866/9354) participants were the Three Gorges migrants. Serum uric acid level in men was significantly higher than that in women [(285.1 ± 80.2) µmol/L vs. (210.3 ± 65.0) µmol/L,P < 0.01].Serum uric acid level increased significantly in both genders in proportion to increase of age, and was higher in men than in women in all age groups (all P < 0.01). The age-adjusted prevalence was significantly higher in men than in women (5.6% vs. 3.3%, P < 0.01), and was also higher in men aged 35-44 and aged 45-54 than in women (both P < 0.01). There was no significance in prevalence of hyperuricemia in both men and women aged 55-64 and aged ≥ 65. After adjusting age, gender, educational level, migration and occupation, the multiple logistic regression analysis showed that the prevalence of hyperuricemia was higher in alcohol drinking participants than that of non-alcohol drinking participants (OR = 2.06, 95%CI:1.59-2.67, P < 0.01), and in participants used to consume less green vegetables and fruits than in participants consuming more green vegetables and fruits (OR = 1.77, 95% CI:1.27-2.47, P < 0.01).
CONCLUSIONSThe prevalence of hyperuricemia is relatively low in rural area of the Three Gorges.Alcohol drinking and low intake of green vegetables and fruits are the risk factors of hyperuricemia in this population.
Adult ; Aged ; China ; epidemiology ; Cross-Sectional Studies ; Female ; Humans ; Hyperuricemia ; epidemiology ; Logistic Models ; Male ; Middle Aged ; Risk Factors
6.Gene cloning, expression and characterization of a novel phytase from Hafnia alvei.
Wei-Na GU ; Huo-Qing HUANG ; Pei-Long YANG ; Hui-Ying LUO ; Kun MENG ; Ya-Ru WANG ; Bin YAO
Chinese Journal of Biotechnology 2007;23(6):1017-1021
A gene appA encoding a novel phytase was firstly cloned from Hafnia alvei by PCR and sequenced. The gene was consisted of 1335 bp, encoding 444 amino acids. The calculated molecular weight of the mature APPA was about 45.2 kD. The gene appA was expressed in E. coli BL21 (DE3). Recombinant APPA was purified and its enzymatic properties were determined. The optimum pH for the enzyme was 4.5 and the optimum temperature was 60 degrees C. The pH stability of r-APPA is good, the relative phytase activity was above 80% after treated in buffers of pH 2.0-10.0. The specific activity of r-APPA is 356.7 U/mg, and the Km value was 0.49 mmol/L and Vmax of 238 U/mg. The enzyme showed resistance to pepsin and trypsin treatment.
6-Phytase
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biosynthesis
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genetics
;
isolation & purification
;
Amino Acid Sequence
;
Cloning, Molecular
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Escherichia coli
;
genetics
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metabolism
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Hafnia
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enzymology
;
genetics
;
Hydrogen-Ion Concentration
;
Molecular Sequence Data
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Recombinant Proteins
;
biosynthesis
;
genetics
;
isolation & purification
;
metabolism
;
Temperature
7.Mutation research on Q23L and Q23LG272E in phytase derivated from Aspergillus fumigatus.
Wei-Na GU ; Pei-Long YANG ; Ya-Ru WANG ; Hui-Ying LUO ; Kun MENG ; Ning-Feng WU ; Bin YAO ; Yun-Liu FAN
Chinese Journal of Biotechnology 2007;23(2):273-277
Aspergillus fumigatus wild-type phytase has many favorable properties, such as a good thermorstability and a broad pH optimum. However, the specific activity of the enzyme is relative low. A. fumigatus Q23L phytase resulted in a remarkable increase in specific activity around pH4.5 - 7.0, but the pH stability of Q23L was lower than A. fumigatus wild-type phytase. To increase the pH stability of Q23L, the mutant Q23LG272E was constructed by site-directed mutagenesis with PCR. The gene of A. fumigatus wild-type phytase and the mutant genes encoding the Q23LG272E and the Q23L were correctly expressed in Pichia pastoris GS115. Enzymes were purified and their enzymatic properties were determined. The results revealed that the specific activity of the Q23L improved remarkably, which increased from 51 u/mg of the wild type to 109 u/mg at pH5.5. Meanwhile, the pH stability of Q23L, decreased evidently, especially from pH3.0 to pH4.0.The pH stability of Q23LG272E in pH3.0 - 4.5 and pH6.5 - 7.0 has been improved compared with Q23L. The specific activity of Q23LG272E basically maintained at the level of Q23L. Analysis of 3-D structure and sequence similarity were used to reveal the presumable factors influencing the enzymatic properties of Q23LG272E, and discussion for the relationship between structure and function of phytase was given.
6-Phytase
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chemistry
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genetics
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metabolism
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Amino Acid Substitution
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Aspergillus fumigatus
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enzymology
;
genetics
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Biocatalysis
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Electrophoresis, Polyacrylamide Gel
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Fungal Proteins
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chemistry
;
genetics
;
metabolism
;
Hydrogen-Ion Concentration
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Models, Molecular
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Mutagenesis, Site-Directed
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Mutant Proteins
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genetics
;
metabolism
;
Mutation
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Pichia
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genetics
;
Polymerase Chain Reaction
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Protein Conformation
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Protein Engineering
;
methods
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Recombinant Proteins
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metabolism
;
Structure-Activity Relationship
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Substrate Specificity
8.Diagnostic value for detection of aspergillus ribosomal RNA in fungal rhinosinusitis with hybridization in situ.
Yuan LI ; Yang LIU ; Na-Ya WANG ; Da-Zhang YANG ; Qiu-Hang ZHANG ; Yue WANG ; Jie LUO
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2007;42(11):852-855
OBJECTIVETo develop a reliable, rapid assay for detecting pathogenic aspergillus species in fungal sinusitis.
METHODSThirty-seven formalin-fixed and paraffin-embedded surgical tissue specimens from patients with fungal sinusitis were used in the present study. The aspergillus specific oligonucleotide probe was designed, commercially synthesized, and digoxigenin-labeled. Twenty-three-base oligonucleotides was selected that was complementary to 18S ribosomal RNA sequences (18S-1 probe) for detecting medically important aspergillus species.
RESULTSIn situ hybridization for aspergillus rRNA was positive in 28 cases with the 18S-1 probe. Compared with HE (21) and methenamine-silver stain (23).
CONCLUSIONIn situ hybridization provides rapid and accurate identification for fungal organism in tissues, and may be useful if cultures are negative or have not performed.
Adult ; Aged ; Aspergillosis ; diagnosis ; Aspergillus ; genetics ; isolation & purification ; Female ; Humans ; In Situ Hybridization ; Male ; Middle Aged ; RNA, Fungal ; isolation & purification ; RNA, Ribosomal, 18S ; isolation & purification ; Sinusitis ; diagnosis ; microbiology
9.Cyclophosphamide-induced ovarian damage and stem cell factor expression in rat ovaries.
Lu LUO ; Dong-Zi YANG ; Zhen WANG ; Qing-Xue ZHANG ; Ya-Qin MO ; Na DI
Journal of Southern Medical University 2007;27(10):1476-1479
OBJECTIVETo investigate the possible pathways for ovarian injury after administration of cyclophosphamide in rats.
METHODSAdult SD rats received a single injection of saline vehicle or chemotherapeutic agent cyclophosphamide, and 8 weeks later, the ovaries were removed, fixed and serially sectioned for pathological examination and ovarian follicle counting. The expression of stem cell factor (SCF) protein was evaluated by immunohistochemistry and immunoreactive score, and SCF mRNA expression determined by RT-PCR in rat ovaries.
RESULTSCyclophosphamide had a detrimental effect on ovarian stromal function and lead to primordial follicle loss. Immunoreactive SCF antigens were expressed on the oocytes in the primordial and primary follicles of rat ovaries, and also in the granulosa cells of the secondary follicles and early antral follicles. There was a higher granulosa SCF, lower oocyte SCF and higher SCF mRNA level in the ovaries of the rats exposed to cyclophosphamide as compared with those in control rat ovaries (P <0.05).
CONCLUSIONAltered SCF expression in the ovaries of rats exposed to cyclophosphamide can be helpful for understanding the mechanisms for chemotherapeutic drug-induced ovarian damage.
Animals ; Cyclophosphamide ; adverse effects ; Female ; Gene Expression ; drug effects ; Granulosa Cells ; drug effects ; metabolism ; Oocytes ; drug effects ; metabolism ; Ovary ; cytology ; drug effects ; injuries ; metabolism ; Rats ; Rats, Sprague-Dawley ; Stem Cell Factor ; genetics ; metabolism
10.SOCS3 expression correlates with severity of inflammation in mouse hepatitis virus strain 3-induced acute liver failure and HBV-ACLF.
Yong, LI ; Mei-Fang, HAN ; Wei-Na, LI ; Ai-Chao, SHI ; Yuan-Ya, ZHANG ; Hong-Yan, WANG ; Fa-Xi, WANG ; Lan, LI ; Ting, WU ; Lin, DING ; Tao, CHEN ; Wei-Ming, YAN ; Xiao-Ping, LUO ; Qin, NING
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(3):348-53
Recently, suppressor of cytokine signaling-3 (SOCS3) has been shown to be an inducible endogenous negative regulator of Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway which is relevant in inflammatory response, while its functions in acute liver failure and HBV-induced acute-on-chronic liver failure (HBV-ACLF) have not been fully elucidated. In this study, we explored the role of SOCS3 in the development of mouse hepatitis virus strain 3 (MHV-3)-induced acute liver failure and its expression in liver and peripheral blood mononuclear cells (PBMCs) of patients with HBV-ACLF. Inflammation-related gene expression was detected by real-time PCR, immunohistochemistry and Western blotting. The correlation between SOCS3 level and liver injury was studied. Our results showed that the SOCS3 expression was significantly elevated in both the liver tissue and PBMCs from patients with HBV-ACLF compared to mild chronic hepatitis B (CHB). Moreover, a time course study showed that SOCS3 level was increased remarkably in the liver of BALB/cJ mice at 72 h post-infection. Pro-inflammatory cytokines, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, were also increased significantly at 72 h post-infection. There was a close correlation between hepatic SOCS3 level and IL-6, and the severity of liver injury defined by alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, respectively. These data suggested that SOCS3 may play a pivotal role in the pathogenesis of MHV-3-induced acute liver failure and HBV-ACLF.