1. The value of adenosine deaminase, interferon-gamma, and interferon-gamma induced protein of 10KD in the diagnosis of tuberculous pleuritis
Medical Journal of Chinese People's Liberation Army 2015;40(6):458-462
Objective To explore the value of adenosine deaminase (ADA) activity, interferon-gamma (IFN-γ) and IFN-γ induced protein of 10kD (IP-10) levels in pleural effusion for the diagnosis of tuberculous pleuritis. Methods ADA activity, IFN-γ and IP-10 levels in pleural effusion were determined in sixty-three patients with tuberculous pleuritis and 50 patients with malignant pleural effusion. Results The mean levels of ADA, IFN-γ and IP-10 in the tuberculous pleural effusion were significantly higher than those in malignant pleural effusion (P<0.01). When 45U/L was regarded as cut off value for ADA, the sensitivity, specificity and diagnostic odds ratio in the diagnosis of tuberculous pleurisy were 71.4%, 94.0% and 39.17 respectively. When 138.5pg/ml was regarded as cut off value for IFN-γ in tuberculous pleural effusion, the sensitivity, specificity and diagnostic odds ratio were 93.7%, 82.0% and 67.19 respectively. When 9.21μg/ml was regarded as cut off value for IP-10 in tuberculous pleural effusion, the sensitivity, specificity and diagnostic odds ratio were 85.7%, 90.0% and 54.00 respectively. The combined determination of the three markers for the diagnosis of tuberculous pleurisy had a sensitivity of 95.2%, specificity of 96.0% and diagnostic odds ratio of 72.16. Conclusion The accuracy of diagnosis for tuberculous pleurisy can be improved by combined determination of ADA, IFN-γ and IP-10.
2.Identication of pearl powder and conch powder from different origins by differential scanning calorimetry.
Jia CHEN ; Ming-hua LI ; Kun-zi YU ; Ya-juan DONG ; Nan-ping ZHANG ; Xiao-ru HU ; Feng WEI ; Shuang-cheng MA
China Journal of Chinese Materia Medica 2015;40(8):1459-1462
The paper is aimed to establish a methods for identication of pearl powder and conch powder from different origins. Hermetic aluminum pan was used to encapsulate samples. The optimal testing conditions were: heating rate 10 degrees C x min(-1), sample weight 3 mg and nitrogen gas flow rate 40 mL x min(-1). The enthalpy values of pearl powder and conch powder was obvious different. Identication of pearl powder and conch powder by DSC is a practical method for its accuracy, convenience and practificality.
Animal Shells
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chemistry
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Animals
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Calorimetry, Differential Scanning
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methods
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China
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Discriminant Analysis
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Pinctada
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chemistry
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classification
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Powders
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chemistry
3.Identification of Max binding protein as a novel binding protein of Nck1 and characterization of its role in inhibiting human liver cancer SK-HEP-1 cells.
Qi ZHOU ; Tao HUANG ; Ya-feng WANG ; Kun-sun ZHANG ; Dong CHEN ; Bao-gang PENG
Chinese Medical Journal 2012;125(18):3336-3339
BACKGROUNDThe tendency of tumor cells to disperse throughout the liver is a distinct feature of hepatocellular carcinoma (HCC). Nck family adaptor proteins function to regulate actin cytoskeletal reorganization that leads to cell motility. We previously found that Max binding protein (MNT) was differentially expressed in HCC, and interacted with Nck1 by 2-DE. MNT is a protein member of the Myc/Max/Mad network which plays roles in cell proliferation, differentiation, and death. We investigated the effects of MNT on migration of human liver cancer SK-HEP-1 cells to study the migration regulatory role of MNT in HCC cells.
METHODSInteraction between MNT and Nck1 was further validated in hepatoma cells by GST-pull down assay and immunoprecipitation. siRNAs specific to MNT (MNT siRNA) were used to knockdown MNT expression. Western blotting, transwell assay were used to determine the migration potential of cells.
RESULTSInteraction between MNT and Nck1 was validated in hepatoma cells. MNT knockdown promoted the migration of human liver cancer SK-HEP-1 cells (P < 0.01).
CONCLUSIONThe results suggest that MNT, via interaction with Nck1, inhibits hepatoma cell migration.
Adaptor Proteins, Signal Transducing ; genetics ; metabolism ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ; genetics ; metabolism ; Blotting, Western ; Cell Differentiation ; genetics ; physiology ; Cell Line, Tumor ; Cell Movement ; genetics ; physiology ; Humans ; Immunoprecipitation ; Liver Neoplasms ; Oncogene Proteins ; genetics ; metabolism ; Protein Binding ; genetics ; physiology ; Repressor Proteins ; genetics ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction
4.Determination of the fluorine release of fluoride varnishes by neutron activation method.
Ya-kun PING ; Hong-ying LIU ; Dong-mei LIU ; Lan-ying GUO
Chinese Journal of Stomatology 2006;41(11):664-665
OBJECTIVETo determine the fluorine content in enamel before and after besmearing fluoride varnishes by neutron activation method.
METHODSA pair of mandibular deciduous central incisor teeth were chosen. One was removed, and the other was besmeared with fluoride varnishes and removed 24 hours later. The contents of fluorine were determined and analyzed statistically.
RESULTSThe fluorine content in the enamel of the tooth besmeared with fluoride varnishes was higher than that in the control tooth (P < 0.05).
CONCLUSIONSThe application of the fluoride varnishes can effectively increase the content of fluorine in the sclerous tissues.
Cariostatic Agents ; pharmacokinetics ; Child ; Dental Caries ; prevention & control ; Dental Enamel ; chemistry ; Female ; Fluorides ; analysis ; Fluorides, Topical ; pharmacokinetics ; Humans ; Male
5.Pharmacokinetic study of recombinant human acidic fibroblast growth factor in rabbits by skin external use.
Xiao-kun LI ; Hua XU ; Wen ZHAO ; Qing ZHENG ; Ya-dong HUANG ; Xiao-ping WU ; Chang-zheng LIU
Acta Pharmaceutica Sinica 2002;37(6):424-427
AIMTo investigate the pharmacokinetic characteristics of recombinant human acidic fibroblast growth factor (rhaFGF) after external use in rabbits.
METHODS125I-rhaFGF 180 U.cm-2 was daubed to normal skin and scathed skin in rabbits. The radioactivity and paper chromatography were used to determine the 125I-concentrations and distribution in plasma and organs at different times.
RESULTSThe plasma concentration of 125I-rhaFGF increased rapidily, and reach peak plasma level (73.03 pg.mL-1) thirty minutes after administration. Then the concentration of 125I-rhaFGF decreased quickly after thirty minutes, and approached to zero after three hours. Highest radioactivity accumulated in the skin, followed by kidney, lowest in the brain 96 h after administration.
CONCLUSIONrhaFGF can not be absorbed from the normal skin, whereas a small amount of rhaFGF can be absorbed through scathed skin. The t1/2 of rhaFGF in plasma was very short. Cumulative effect of rhaFGF was not observed. Absorbed rhaFGF showed high affinity to skin, and can be distributed to skin far from the site of administration.
Administration, Cutaneous ; Animals ; Female ; Fibroblast Growth Factor 1 ; administration & dosage ; pharmacokinetics ; Male ; Rabbits ; Recombinant Proteins ; administration & dosage ; pharmacokinetics ; Skin ; injuries ; metabolism ; Skin Absorption ; Tissue Distribution
6.Poroid hidradenoma of the scalp
Byung Duk MIN ; Chong Kun LEE ; Chang Eun CHUNG ; Dong Chul KIM ; So Ya PAIK
Archives of Craniofacial Surgery 2021;22(3):154-156
Poroid hidradenoma has both features of hidradenoma and poroma. The histological hidradenoma framework consisting of solid and cystic components, and the presence of poroid and cuticular cells resembling a poroid neoplasm. Despite transforming into malignant neoplasm only in < 1% of cases, its histological characteristics may resemble those of malignant neoplasms. Although the risk of malignant transformation is very low, surgical excision is recommended to prevent growth and/or recurrence. To date, very few cases of poroid hidradenoma have been reported in the literature. Herein, we present a case of poroid hidradenoma on the scalp of a 74-year-old woman.
7.Poroid hidradenoma of the scalp
Byung Duk MIN ; Chong Kun LEE ; Chang Eun CHUNG ; Dong Chul KIM ; So Ya PAIK
Archives of Craniofacial Surgery 2021;22(3):154-156
Poroid hidradenoma has both features of hidradenoma and poroma. The histological hidradenoma framework consisting of solid and cystic components, and the presence of poroid and cuticular cells resembling a poroid neoplasm. Despite transforming into malignant neoplasm only in < 1% of cases, its histological characteristics may resemble those of malignant neoplasms. Although the risk of malignant transformation is very low, surgical excision is recommended to prevent growth and/or recurrence. To date, very few cases of poroid hidradenoma have been reported in the literature. Herein, we present a case of poroid hidradenoma on the scalp of a 74-year-old woman.
8.Solitary sclerotic fibroma on the thigh
Ji Hoon KIM ; Chi Ho SHIN ; Dong Chul KIM ; So Ya PAIK ; Chong Kun LEE
Archives of Craniofacial Surgery 2020;21(5):323-325
Sclerotic fibroma is a rare fibrous tumor of the skin associated with Cowden’s disease. In 1989, Rapini described sclerotic fibroma without Cowden’s disease as solitary sclerotic fibroma of the skin. It is a solid, well-circumscribed, slow-growing nodular tumor and it looks similar to a keloid scar. Consequently, it is extremely difficult to make a differential diagnosis of solitary sclerotic fibroma with keloid scar based on clinical findings only. The authors report a case of solitary sclerotic fibroma arising at the left lateral thigh of a 25-year-old man.
9.Clinical trial of Qishen Tongluo granules in the treatment of diabetic nephropathy in stage Ⅲ
Ying-Chun CHEN ; Zhong-Xiang LIU ; Ya-Mei WANG ; Yan-Hong LI ; Yan-Fei DONG ; Cong-Kun XIANG
The Chinese Journal of Clinical Pharmacology 2018;34(7):777-779
Objective To observe the clinical efficacy and safety of Qishen Tongluo granules in the treatment of diabetic nephropathy (DN) in stage Ⅲ.Methods One hundred and four patients with DN in stage Ⅲ were randomly divided into control and treatment groups with 52 cases per group.Control group was given potassium choride tablets 100 mg per time,qn,orally.Treatment group was given Qishen Tongluo granule 20 g per time,bid,orally.Two groups were treated for 12 weeks.The clinical efficacy,fasting plasma glucose (FPG),hemoglobin A1 c (HbA1c),homostasis model assessment of insulin resistance index (HOMA-IR),urinary albumin excretion rate (UAER),serum creatinine (SCr) and adverse drug reactions (ADR) were compared between two groups.Results After treatment,the total effective rates of treatment and control groups were 86.54% (45 cases/52 cases) and 69.23% (36 cases/52 cases) with significant difference (P < 0.05).After treatment,the main indexes in treatment and control groups were compared:FPG were (6.54±0.68) and (7.16 ±0.74) mmol · L-1,HbA1c were (7.18 ±0.72)% and (7.62-± 0.79)%,HOMA-IR were (4.71 ±1.06) and (5.65±1.10),UAERwere (58.34 ±16.52) and (82.15±19.05) mg· 24 h-1,SCr were (85.60 ± 15.69) and (94.25 ± 16.77) μmol · L-1,the differences were statistically significant (all P <0.01).The adverse drug reactions in two groups were nausea,abdominal discomfort,lack of appetite and dizziness.The total incidences of ADR were 9.62% and 13.46% without significant difference (P > 0.05).Conclusion Qishen Tongluo granules have a definitive clinical efficacy and safety in the treatment of DN in stage Ⅲ,which can effectively improve the renal function and insulin resistanc,and reduce blood glucose.
10.Feasibility and clinical application of design about the hollowed titanium stick supporting the femoral head and preventing it from collapsing.
Xin-ming YANG ; Wei SHI ; Ya-kun DU ; Lei ZHANG ; Xian-yong MENG ; Yao-yi WANG ; Wei-Dong ; Zhen-shun HU
China Journal of Orthopaedics and Traumatology 2011;24(7):564-569
OBJECTIVETo evaluate the feasibility and the clinical practicality of the design about the hollowed titanium stick supporting the femoral head and preventing it from collapsing.
METHODSFrom Jan.2003 to Jun.2007, 36 patients (46 hips) diagnosed as cystis degeneration of the femoral head were treated by surgical operation, including 20 males and 16 females with an average age of 40 years ranging from 18 to 56 years old, and the course of the disease was from 10 to 24 months (16 months on average). According to ARCO staging,there were 24 patients (34 hips) in NFH II, of which 11 hips were II a stage, 13 hips were II b stage and 10 hips were II c stage; there were 6 patients diagnosed as osteofibrous,4 patients as simple bone cyst and 2 patients as chondromyxoid fibroma. Under X-ray the percutaneous narrow core decompression and focus infection elimination were performed and supported the sclerotin under the cartilage with titanium stick. The patients were followed-up at the first, third, sixth, twelfth, twenty-fourth and thirty-sixth month after the operation. The clinical evaluation was done by X-ray and the indexes included stable, unstable and abortive. The data was analyzed by Fisher exact probility and the suviaval rate was analyzed by Kaplan-Meier suviaval curve using statistical soft ware SPSS13.5.
RESULTSThere were no unstable or failure cases on each period from the 1st month to the 12th month after the operation, indicating that the supporting effect of the titanium stick was exact during 12 months after the operation. There were unstable and failure cases from the 12th month to the 24th month after the operation, which were mainly in stage NFH II c but the comparision of the stable rate in this period and 12 months after the operation had no obvious statistical differences (P>0.05) indicating that the supporting effect of the titanium stick was feasible during the stage. One of the unstable cases deteriorated and failed but there were no new unstable cases, both the stable rate and the unstable rate had no change and the failure rate rose on the 36th month after the operation. The compar- ision of the stable rate on each period after the operation had no obvious difference (P=0.197>0.05), which indicated that the supporting effect of the titanium stick was persistent. By the difference of the etiology the three-year survival rate of the relevant NFH II c pathological changes was the lowest-70% and the survival rate of the pathological changes induced by other etiological factors was 90.2%.
CONCLUSIONThe design about the hollowed titanium stick supporting the collapsed femoral head is feasible. Using the hollowed titanium stick to support the femoral head and prevent it from collapsing is pragmatic in the clinical and the effect is positive, however, when it comes to the NFH II c pathological changes, the choice should be made discreetly.
Adolescent ; Adult ; Feasibility Studies ; Female ; Femur Head ; diagnostic imaging ; surgery ; Follow-Up Studies ; Humans ; Internal Fixators ; adverse effects ; Male ; Middle Aged ; Prosthesis Design ; methods ; Titanium ; Tomography, X-Ray Computed ; Young Adult