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Background Econazole nitrate is not effective as an antifungal eyedrop because of its poor intraocular permeability,therefore changing the formulation of econazole nitrate to improve its intraocular permeability become a critical point in the treatment of intraocular fungal infection. Objective The present study was to observe the penetration of 0.5％ econazole nitrate nanoparticles in the corneas and aqueous humors following its topicaladministration. Methods Econazole nitrate nanoparticles were prepared by quasi-emulsion solvent diffusion.Characteristics and size of nanoparticles were examined with transmission electron microscope and laser scatteringmethod,respectively.Econazole nitrate nanoparticles drops (0.5％ )was topically administered in 27 New Zealandwhite rabbits bilaterally,and aqueous humor and corneas were obtained after the application of the eye drops for 5,15,30,45,60,90,120,180,240 minutes respectively to detect the concentration of econazole nitrate with highperformance liquid chromatography (HPLC). The pharmacokinetic parameters were calculated with 3 p97pharmacokinetic computer software.The use of the animals followed the Regulation for the Administration of AffairsConcerning Experimental Animals by State Science and Technology Commission. Results The diameter of thenanoparticles was 50 nm with the round shape and encapsulation efficiency was 96.0％.Econazole nitrate nanoparticlesat the concentration of 0.5％ could be rapidly separated with other elements by HPLC with a lowest quantitativeconcentration of 0.1 mg/L.The mean recovery rates of econazole nitrate nanoparticles were 98.09％ in cornea and 99.66％ in aqueous humor,respectively after topical administration.The peak levels of econazole nitrate nanoparticles in cornea and aqueous humor were achieved at 5 minutes after application ( cornea:40.620 μg/g± 7.756 μg/g;aqueous humor:0.504 mg/L±0.153 mg/L),and its half-life( t1/2 )in cornea and aqueous humor was 23.5 minutes and 18.6 minutes,respectively. Conclusions Econazole nitrate nanoparticles at 0.5％ concentration can remain a feasible bioavailability in ocular tissue and therapeutic level in cornea and aqueous humor.

4.Thrombotic thrombocytopenic purpura: an autopsy report with literature review.

Wei-Ya WANG ; Jie JI ; Wei-Ping LIU

Chinese Journal of Pathology 2008;37(3):208-210

5.Research and determination of occupational exposure limits of nitroguanidine in air of working places.

Wen-xia DU ; Ya-jie LIU ; Yan-qi WANG

Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(5):380-382

6.Allelopathic effects of extracts from tuberous roots of Aconitum carmichaeli on three pasture grasses.

Yu-jie JIAO ; Ya-qi WANG ; Ling YUAN

China Journal of Chinese Materia Medica 2015;40(21):4155-4159

The tuberous roots of Aconitum carmichaeli are largely used in traditional Chinese medicine and widely grown in Jiangyou, Sichuan, China. During the growth process, this medicinal plant releases a large amount of allelochemicals into soil, which retard the growth and development of near and late crops. Therefore, a pure culture experiment was thus carried out by seed soaking to study the allelopathic effects of extracts from tuberous roots of A. carmichaeli (ETR) on the seed germination and young seedling growth of Lolium perenne, Trifolium repens, and Medicago sativa, the late pasture grasses after cultivation of A. carmichaeli. The results showed that three pasture grasses varied significantly in seed germination and young seedling growth in response to ETR concentrations. Seed germination of M. sativa was stimulated by low ERT concentration (0.01 x g(-1)), while all of pasture grass seeds germinated poorly in solution with 1.00 g x L(-1). Seed soaking with 1.00 g x L(-1) also inhibited significantly the growth of pasture young seedlings, with M. sativa showing the highest seedling height reduction of 42.05% in seeding height, followed by T. repens (40.21%) and L. perenne with about 11%. Cultivation of L. perenne could thus be beneficial to increase whole land productivity in A. carmichaeli-pasture grass cropping systems. In addition, hydrolysis of protein, starch, and inositol phosphates was blocked and free amino acids, soluble sugars and phosphorus were decreased in seeds by seed soaking with ETR, which could be one of the reason for the inhibition of seed germination. There was a significant reduction in root vigor, nitrate reductase, and chlorophyll after the seed treatment with ETR, indicating the suppression of nutrient uptake, nitrate assimilation, and photosynthesis by allelopathic chemicals in ETR, which could lead to the slow growth rate of pasture grass seedlings.
Aconitum ; chemistry ; metabolism ; Allelopathy ; China ; Pheromones ; metabolism ; pharmacology ; Plant Extracts ; metabolism ; pharmacology ; Plant Roots ; chemistry ; metabolism ; Poaceae ; drug effects ; growth & development

10.Effect of Local Intramuscular Repeated Injection with Botulinum Toxin-A for Treatment of Spastic Cerebral Palsy

ya-jie, WANG ; bao-qin, GAO ; wei-li, YANG

Journal of Applied Clinical Pediatrics 2004;0(08):-

1.How to optimize the clinical therapeutic protocol for triple-positive breast cancer.

2.Research advancement in pathogenicity island and the pathogenic bacteria of periodontitis.

3.Pharmacokinetics of topically applied econazole nitrate nanoparticles in rabbit eye

4.Thrombotic thrombocytopenic purpura: an autopsy report with literature review.

5.Research and determination of occupational exposure limits of nitroguanidine in air of working places.

6.Allelopathic effects of extracts from tuberous roots of Aconitum carmichaeli on three pasture grasses.

7.Progress of molecular pathology in pediatric brain tumor.

8.Two patients with subacute 1,2-Dichloroethene poisoning.

9.Mutagenicity and teratogenicity of hexanitrohexaazaisowurtzitane.

10.Effect of Local Intramuscular Repeated Injection with Botulinum Toxin-A for Treatment of Spastic Cerebral Palsy

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