ObjectiveTo explore the characteristics of violence behavior of the psychotics in communities. Methods37 psychotics who used to have violence behavior were investigated with Questionnaire of Characteristics and Associated Factor for Violence Behavior of Psychotics in Communities created ourselves. ResultsThe psychotics suffered violence behavior appeared some peculiarities for the time, place, and dealing with tools, etc. Their mental state, course of disease and treatment were associated with their violence behavior. ConclusionThere is some characteristics in most of the violence behavior of the psychotics, which may be helpful for preventing.

OBJECTIVETo compare the differences of therapeutic effect in lower limb sensation disorder after lumbar disc herniation operation treated with plum-blossom needle along meridians and Methylcobalamin with oral administration, and explore the best time for plum-blossom needle intervention.

METHODSEighty cases of lower limb sensation disorder caused by nucleus pulposus discectomy of lumbar disc herniation were randomly divided into a plum-blossom needle group (40 cases) and a western medication group (40 cases). In plum-blossom needle group, tapping with plum-blossom needle along meridians was received, the meridians located at sensation areas which were dominated by relevant segmental nerve root in operation were selected: the lower limb section of the Spleen Meridian of Foot-Taiyin was selected for operation at L3/L4; the lower limb section of the Gall Bladder Meridian of Foot-Shaoyang was selected for operation at L4 /L5; the lower limb section of the Bladder Meridian of Foot-Taiyang was selected for operation at L5/S1; once treatment was applied every 3 days, and 20 times treatments were applied totally. In western medication group, Methylcobalamin was orally taken for 500 microg, 3 times a day. The recovery of lower limb sensation disorder was evaluated and compared by the comprehensive evaluation method of sensory function of body nerve after 2 months in both groups.

RESULTSThe total effective rate was 90.0% (36/40) in plum-blossom needle group, superior to that of 60.0% (24/40) in western medication group (P < 0.05). In plum-blossom needle group, the effect for the cases which received treatment within one month after operation was superior to that received treatment during 1-3 months after operation (P < 0.05); and there was no significant differences between the cases which received the treatment during 1-3 months after operation and more than 3 months after operation (P > 0.05).

CONCLUSIONThe therapeutic effect of lower limb sensation disorder after lumbar disc herniation operation treated with plum-blossom needle along meridians is superior to that of Methylcobalamin with oral administration; the plum-blossom needle intervention within one month after operation can receive the best effect.

Acupuncture Therapy ; instrumentation ; Adult ; Aged ; Female ; Humans ; Intervertebral Disc Displacement ; complications ; surgery ; Lower Extremity ; physiopathology ; Male ; Meridians ; Middle Aged ; Sensation ; Sensation Disorders ; therapy

The aim of the present study was to gain an insight into the thiopurine methytransferase (TPMT) genotyping assay, which was based on polymerase chain reaction (PCR), allele-specific PCR, restriction digestion of PCR products, denaturing high-performance liquid chromatography (DHPLC) and SNaPshot sequencing and in combination with direct DNA sequencing. Among the f our methods to test TPMT genetic SNPs based on PCR, allele specific PCR was not able to differentiate wild type from varied type. BsiYI, MwoI and AccI to digest PCR products were used so that SNP in TPMT exon 5, 7 and 10 tested. It showed that there were no differences between the results of digestion of PCR products and those of DNA sequence analysis. Therefore, this method was reliable. But some other methods were still needed to look for a compensation, because no restriction map changing resulted from the 2 SNPs in TPMT promotor was found. As to the results of DHPLC, those for the screening of TPMT exon-5 and -10 for SNPs were the same as restriction analysis of PCR products and direct DNA sequencing. But the variation of the heterozygotes in exon-7 was high, which was different from the results of direct DNA sequencing. After changing the Tm of DNA step by step, It was found that all the samples showed single peak when the temperature was 54 degrees C. But this result was unbelievable because a heterozygote in exon 7 as positive control could not be found. Therefore, it was necessary to test the sensitivity and accuracy of DHPLC, though DHPLC could be used as an effective method of SNPs screening. The results of the SNaPshot sequencing were also same as those of restriction analysis of PCR products and direct DNA sequencing. And the results showed that the bases of TPMT promoter -91 and -168 were G, instead of A and T. The results of the four methods to detect TPMT genetic SNPs based on PCR showed that SNPs analysis technique should be a combination of the techniques above-mentioned. One technique alone could not satisfy the need in clinics and research. The compensation of each other was very important.
Chromatography, High Pressure Liquid ; Exons ; Humans ; Methyltransferases ; genetics ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide

OBJECTIVETo observe therapeutic effect of orientation taping magnetic granules at auricular points and Neiguan (PC 6) on motion sickness.

METHODSAll 200 cases of motion sickness were divided into a treatment group (n = 102) and a control group (n = 98). The treatment group were treated with orientation taping magnetic granules at auricular points and Neiguan (PC 6), and the control group with oral administration of theohydramine.

RESULTSThere was a significant difference between the two groups in the total effective rate (P < 0.05), and clinical symptoms disappeared more rapidly in the treatment group as compared with the control group (P < 0.05). The therapeutic effect in the mild and moderate patients of the treatment group was better than that of the control group, and there was no significant difference in the therapeutic effect for the severe patients between the two groups. In the treatment group, the therapeutic effect of the mild patient was the best, the moderate patient was next and the severe patient was the worst, with a very significant difference (P < 0.005).

CONCLUSIONOrientation Taping magnetic granules at auricular points and Neiguan (PC 6) has an obvious therapeutic effect on motion sickness, with less adverse reactions.

Acupuncture Points ; Adolescent ; Adult ; Aged ; Child ; Female ; Humans ; Magnetics ; therapeutic use ; Male ; Middle Aged ; Motion Sickness ; prevention & control ; therapy

Objective To explore the relationship between esophagus cancer patients and both environmental and genetic factors,through analyzing the data on birth orders from esophagus cancer patients of Shanxi province.Methods Both Greenwood and Haldane methods on birth order were used to study the 1101 cases with esophagus cancer from Shanxi province.All the patients had received surgery and were diagnosed,by pathological evidence.First certificates of the patients were confirmed through the standard genetic epidemiologic investigation.Birth order was investigated on probands of the 1101 cases with esophagus cancer and their 44 siblings.Results Results form the Greenwood method showed that there was a tendency for cases with esophagus cancer in birth orders First to Third.However,the Haldane method showed that the results were quite different between actual value and the average theory value of 6A (6A(actual value)=17 118,(X)6A(average theory value) =19 290,X=∣6A-(X)6A∣/√V6A =7.63,X ＞ 2) which suggested that the birth order had some effects on the occurrence of esophagus cancer.In addition,the actual value of 6A was lower than the theoretic average value,and the parents at younger productive age or baby at the first birth was easy to develop esophagus cancer.Conclusion Esophagus cancer was related with the birth order,especially at early order,which was not consistent with the national reports on esophagus cancer.Results from this study suggested that there were certain effects of environmental risk factors on esophagus cancer patients.

Objective] To evaluate effect of health literacy intervention in occupational groups , explore suitable ways and provide suggestions for workplace health promotion . [ Methods ] In application of convenience sampling , Minhang District , Hongkou District and Qingpu District each selected 2 enterprises respectively according to their type , scale and location , setting one as intervention enterprise and the other as control .Then a six-month comprehensive intervention was conducted among intervention groups .Two hundred employees in each enterprise were randomly investigated before and after intervention to evaluate the effect . [ Results ] The overall health literacy level of participants in the intervention group rose from 16.7% to 33.4%, with improvement on the literacy levels of scientific view , infectious diseases prevention and treatment , chronic disease control and prevention and the three aspects of health literacy content ( P<0 .05 ) , while such improvement was not reflected in the control group , and health skill literacy, safety and first aid literacy declined (P <0.05). [Conclusion] Comprehensive intervention based on health needs could improve the level of health literacy of occupational groups . Intervention in future should focus on improving the literacy of chronic disease control and prevention and basic medical care in occupational population .

Objective To explore the effect of the classified management intervention model on the smoking status of high risk population of chronic non-communicable diseases in institutions from Chongqing, so as to provide scientific basis for the management of high risk population of chronic non-communicable diseases. Methods Workers from governments and institutions of four districts in Chongqing were sampled by cluster sampling. High risk population of non-communicable disease were screened and enrolled into the intervention group and control group. The intervention group were intervened by classified management. And the control group weren’t intervened. To evaluate the change of smoking and smoking hazard cognition in intervention group and control group after one year intervention. Results The result of analysis of the net effect of intervention measures using difference in difference regression model showed that the intervention measures could reduce the smoking rate, the current smoking rate and the daily smoking rate in subjects (OR=0.32, 95% CI: 0.21-0.49; OR=0.31, 95% CI: 0.20-0.48; OR=0.34, 95% CI: 0.21-0.54). The intervention measures could increase the awareness rates of smoking causing serious diseases, stroke and lung cancer in subjects (OR=2.19, 95% CI: 1.08-4.42; OR=1.73, 95% CI: 1.17-2.57; OR=2.17, 95% CI: 1.25-3.77), the difference was statistically significant (all P<0.05). Conclusion The intervention model of classified management for high risk population of chronic non-communicable diseases in institutions can effectively improve the awareness rate of smoking hazards among the subjects, and then reduce the smoking rate. Therefore, this intervention model is worth popularizing and applying.

OBJECTIVEThe aim of the study was to determine the prevalence and the distribution pattern of lesion site of intracranial vascular stenosis and to identify risk factors for the stenosis in patients with essential hypertension.

METHODSA total of 231 consecutive inpatients with essential hypertension were included in this study. Patients with the history of cerebrovascular diseases and relevant neurological symptoms were excluded. Intracranial vascular stenosis (>50% diameter reduction) was detected using CT angiography (CTA).

RESULTSOf 231 patients, 69 (29.87%) had intracranial artery stenosis. The most common stenosis site is middle cerebral artery (43.69%), followed by carotid siphon (20.39%). The stenosis in internal carotid arterial system (78.64%) was more common than in vertebrobasilar arterial system (21.56%, P < 0.05). The patients with intracranial vascular stenosis were older, had longer history of hypertension, higher levels of systolic blood pressure, higher plasma cholesterol, higher LDL-C. Lp (a), higher urinary microalbumin excretion, thicker ventricular septum, and lower levels of HDL-C than the patients without stenosis. Logistic analysis showed that systolic blood pressure (OR 1.650, 95% CI 1.134 - 2.400, P = 0.023), course of hypertension (OR 1.238, 95% CI 1.072 - 1.429, P = 0.006), LDL-C (OR 2.103, 95% CI 1.157 - 3.823, P = 0.014) and type 2 diabetes (OR 2.325, 95% CI 1.161 - 4.341, P = 0.011) were the independent risk factors of asymptomatic intracranial arterial stenosis.

CONCLUSIONSNearly 30% inpatients with essential hypertension had asymptomatic intracranial artery stenosis. The most common site of stenosis was middle cerebral artery. Hypertension, dyslipidemia and diabetes were risk factors for the development of intracranial arterial stenosis.

Aged ; Cerebral Angiography ; Female ; Humans ; Hypertension ; epidemiology ; pathology ; Intracranial Arterial Diseases ; epidemiology ; Male ; Middle Aged ; Prevalence ; Risk Factors

OBJECTIVETo examine the role of Polo-like kinase 1(PLK1) in the migration and invasiveness of human colorectal cancer cells.

METHODSNine colorectal cancer cell lines were cultured. Cell lines with the highest level of PLK1 expression was selected by PCR and Western blot. Three siRNA oligo segments targeting PLK1 were designed and selected cell lines transfected. Successful transfection was confirmed using real-time PCR and Western blot. Changes in migration and invasiveness of the selected cell line were evaluated by Transwell test.

RESULTSColorectal cancer cell line SW1116 was selected with the highest expression of PLK1 at both mRNA level and protein level. The expression of PLK1 in SW1116 was reduced by the three siRNA oligo segments to varying degrees, and the No.1 siRNA oligo segment was the most efficient. In migration test, the number of cells crossing through chambers in PLK1-siRNA group was 44 ± 14, which was lower than that in the negative control group (242 ± 40) and in blank control group(240 ± 38). In invasion test, the number of cells crossing through chambers in PLK1-siRNA group was 62 ± 3, which was lower than that in negative control group (207 ± 12) and in blank control group (211 ± 15). These differences were statistically significant(P<0.01).

CONCLUSIONPLK1 silencing by siRNA may inhibit the migration and invasiveness of colorectal cancer cells, suggesting that PLK1 might play an important role in the infiltration and metastasis of colorectal cancer.

Cell Cycle Proteins ; genetics ; metabolism ; Cell Line, Tumor ; Cell Movement ; Colorectal Neoplasms ; metabolism ; pathology ; Humans ; Neoplasm Invasiveness ; Protein-Serine-Threonine Kinases ; genetics ; metabolism ; Proto-Oncogene Proteins ; genetics ; metabolism ; RNA, Small Interfering ; genetics ; Transfection

OBJECTIVETo study the expression changes of neuroglobin in rats with the model of diffuse traumatic brain injury and explore the relationship between the neuroglobin and neuron apoptosis in traumatic brain injury.

METHODSThe diffuse traumatic brain injury of rats was induced by the Marmarou's 'weight-drop' device. And the immunohistochemical technique was used to detect the expression changes of neuroglobin and neuron apoptosis in rat brain at different time points post-injury.

RESULTSThe expression of neuroglobin increased twice and reached peaks at 2 hours and 72 hours post-injury respectively. And the increased expression of neuroglobin from 30 minutes to 1 hour post-injury and from 48 hours to 72 hours post-injury accompanied with the decreased expression ratio of Bax to Bcl-2.

CONCLUSIONThe increased expression of neuroglobin in traumatic brain injury informed us that neuroglobin had anti-apoptosis action in post-injury neuron. It could protect the neuron from traumatic stress and secondary ischemia and hypoxia insults during ultra-early and acute stages.

Animals ; Apoptosis ; physiology ; Brain ; metabolism ; pathology ; Brain Injuries ; metabolism ; pathology ; Globins ; metabolism ; Male ; Nerve Tissue Proteins ; metabolism ; Neurons ; pathology ; Random Allocation ; Rats ; Rats, Sprague-Dawley