Objective To explore the expression of vascular endothelial growth factor (VEGF) and apoptosis of the tumor cells in the different regions of rabbit liver VX2 tumor after radiofrequency ablation (RFA).Methods Forty-eight experimental rabbits were implanted with VX2 tumor.After successfully established the model,the rabbits were randomly divided into control group (n=6) and RFA group (n=42).In the RFA group,7 rabbits at each time point were killed at immediately,1 day,2 days,1 week,2 weeks,3 weeks after RFA,and the tumor specimens were retained and performed with HE staining,VEGF,Annexin V-FITC/PI labeling,flow cytometry analysis.The changes of VEGF and apoptosis of the cells in different periods and different zones after RFA were observed.Results After the operation,the difference of the VEGF value of the needle zone,coagulation necrotic zone and junction zone had statistically significant (all P＜0.05).The difference of the VEGF value in each zone between immediately and the other time points after operation by pairwise comparisons were significantly different (all P＜0.05).The VEGF value of the needle zone and coagulation necrotic zone reached the peak after operation immediately,which declined from 1 day to 3 weeks after operative.And VEGF of the junction zone increased from immediately to 1 week after operation,and declined 2 weeks after operation.There were significant differences in the apoptosis rate of the three zones after RFA compared with control group (all P＜0.05).The apoptosis rate in all zones were at the peak on the 1 day after operation,and then showed a downward trend.Conclusion The reduction of VEGF and apoptosis of tumor cells in the needle zone and coagulation necrotic zone are significant,but tumor remnant remains visible in the junction zone.In the third week after RFA,the proliferation of the remaining tumor cells can be recurrent to preoperative levels,which suggests that the further treatment should be performed at this period.

Objective To investigate the effect of activity intervention on intestinal cleansing of patients with cervical cancer undergoing oral administration of compound polyethylene glycol electrolyte powder, so as to improve the intestinal cleanliness of patients and reduce the adverse reactions of drugs. Methods A total of 75 cases of cervical cancer patients were divided into intervention group containing 37 cases and control group containing 38 cases by lottery, the control group before taking medicine was given the diet guidance, explaining the method of taking medicine and time of taking medicine, as well as points for attention after taking medication;while the intervention group increased activity intervention on the basis of the control group. Results The intervention group of patients with intestinal cleanlinessⅠ degree accounted for 78.37％ (29/37), Ⅱ degree (18.91％, 7/37) and Ⅲ degree (2.7％, 1/37); while the control group of patients with intestinal cleanliness Ⅰ degree accounted for 55.3％ (1/38), Ⅱ degree (23.7％, 9/38) andⅢdegree (21.1％, 8/38), and the difference was statistically significant (χ2=6.962, P=0.031).The intervention group with nausea and vomiting level 0 accounted for 59.5％ (22/37), level Ⅰ(24.3％, 9/37) and level Ⅱ(16.2％, 6/37); while the control group with nausea and vomiting level 0 accounted for 28.9％ (11/38), level Ⅰ(44.7％, 17/38), level Ⅱ (26.3％, 10/38), the difference was statistically significant(χ2 = 7.116, P = 0.028). The intervention group with abdominal distension level 0 accounted for 62.2％(23/37), level 1(24.3％, 9/37), level 2 (13.5％, 5/37) and level 3 (0);while the control group with abdominal distension level 0 accounted for 28.9％ (11/38), level 1(47.4％, 18/38), level 2 (21.1％, 8/38) and level 3 (2.6％,1/38), the difference was statistically significant (χ2=8.916, P=0.030). The first defecation time of the intervention group was (2.18 ± 0.59) h and the total defecation time was (2.93 + 0.86) h, while the first defecation time of the control group was (3.13 + 0.64) h and the total defecation time was (3.80+1.01) h, and the difference was statistically significant (t=-3.035,-2.520, P<0.01 or 0.05). Conclusion Activity intervention can improve the intestinal cleansing effect of cervical cancer patients and reduce the occurrence of adverse reactions.

Acute traumatic spinal cord injury (SCI) represents one of the most devastating injuries that afflict the human body. Ascorbic acid ( AA) plays an important role in mammalian central nervous system, especially in SCI. In this study, the change of AA concentration after SCI was investigated by using an on-line electrochemical method integrated with in vivo microdialysis. A microdialysis probe (2 mm in length) was implanted into the spinal cord of an anesthetized rat (Thoracic-10). Microdialysis perfusate (2 μL/ min) was collected in the sample loop of an on-line injector for direct injection onto a glassy carbon electrode which was modified with the heat-treated single-walled carbon nanotubes (SWNTs). Normal ascorbic acid concentration in the extracellular fluids of spinal cords was (26. 17 ± 1. 25) μmol/ L (n =8). The experimental spinal cord injury, induced by a lesion at T-10, significantly increased the extracellular ascorbic acid levels to (53. 24± 1. 95) μmol/ L (n =8). This study provides the experimental evidence on the essential roles of ascorbic acid in spinal cord injuries.

As a multifunctional signal molecule, fibroblast growth factor 21 (FGF21) has been proved to have a variety of biological effects, including anti-inflammatory, antioxidant stress, and neuroprotection. This article reviews the latest research progress on the protective effect of FGF21 in ischemic stroke and its relationship with cognitive impairment.

Vascular cognitive impairment (VCI) is a syndrome from mild cognitive impairment to dementia caused by various vascular risk factors and cerebrovascular diseases. Early detection and definite diagnosis can significantly improve the outcomes of patients with VCI. This article reviews the evaluation and diagnosis of VCI.

Objective To explore the value of serum microRNAs-145(miR-145)and P53 antibody detection in the evaluation of therapeutic effect and prognosis in the treatment of breast cancer with Long Bei Xiaoyao Powder.Methods Ninety cases of breast cancer patients admitted to Beijing Aerospace General Hospital from January 2020 to January 2021 were selected as the study subjects.The patients were randomly divided into an observation group and a control group by random number table method,with 45 cases in each group.All of the patients in the two groups received individualized comprehensive treatment,and additionally the observation group was treated with Long Bei Xiaoyao Powder.The course of treatment covered three months.Real-time fluorescence quantitative polymerase chain reaction(PCR)method was used to detect serum miR-145 expression level,and enzyme-linked immunosorbent assay(ELISA)was used to detect serum P53 antibody expression level.The differences in the pre-and post-treatment expression levels of serum miR-145 and P53 antibodies in the two groups were compared,the relationship between the serum miR-145 and P53 antibody expression level and the poor prognosis of the patients in the observation group was explored by logistic regression analysis,and receiver operating characteristic curve(ROC)was drawn to analyze the value of the serum miR-145 and P53 antibody detection in the prediction of poor prognosis in the observation group.Results(1)After treatment,the detection value of serum miR-145 in the two groups of patients showed an increasing trend(P＜0.05),while that of the P53 antibody showed a decreasing trend(P＜0.05).The intergroup comparison showed that the detection value of serum miR-145 in the observation group was significantly higher than that of the control group,while the detection value of P53 antibody was significantly lower than that of the control group,the differences being all statistically significant(P＜0.05).(2)In the observation group,the detection value of serum miR-145 in the patients with good prognosis was significantly higher than that in the patients with poor prognosis,and the detection value of serum P53 antibody in the patients with good prognosis was significantly lower than that in the patients with poor prognosis,the differences being all statistically significant(P＜0.05).(3)Logistic regression analysis showed that high expression of miR-145 was a protective factor for the patients with poor prognosis in the observation group,and high expression of P53 antibody was a risk factor for the patients with poor prognosis in the observation group(P＜0.05).(4)The area under the ROC curve(AUC)for the prediction of prognosis of the patients in the observation group by the combination of serum miR-145 and P53 antibody was the largest(0.877,95%CI:0.769-0.985),and its sensitivity and specificity were 93.75%and 93.10%,respectively,with statistically significant differences(P＜0.05).Conclusion Low expression of serum miR-145 and high expression of serum P53 antibody are presented in the breast cancer patients with poor prognosis,and Long Bei Xiaoyao Powder exerts satisfactory therapeutic effect in treating breast cancer,which is conducive to enhancing the expression level of serum miR-145 and reducing the expression level of serum P53 antibody.

Objective To investigate the effects of individualized preoperative bowel preparation regimen on intestinal cleansing in patients with cervical cancer. Methods Totally 140 patients who were admitted and diagnosed with cervical cancer in the Department of Oncology of Wuxi Maternal and Child Care Hospital from January 2016 to May 2017 were selected by convenient sampling and randomly divided into a control group and an experimental group by drawing method. Finally, totally 67 patients in each group completed the experiment. Routine preoperative bowel preparation was adopted for patients in the control group, while individualized bowel preparation regimen was adopted for patients in the experimental group, including evaluation before medication, activity guidance for patients with different ages, control of medication speed and response to untoward effects. The intestinal cleansing, untoward effects, time of first defecation and total time of defecation were compared between the patients in the two groups. The rank-sum test, χ2 test and t test were used to analyze the data for the patients in the two groups. Results There was statistically significant difference in the degree of intestinal cleansing between the patients in the two groups (Z=-2.732, P< 0.05); there was statistically significant difference in the degree of nausea and vomiting between the patients in the two groups (Z=-2.065, P< 0.05); there was statistically significant difference in the degree of abdominal distension between the patients in the two groups (Z=-2.526, P<0.05); and there was statistically significant difference in the incidence of stomachache between the patients in the two groups (χ2=5.835, P< 0.05). The time of first defecation and the total time of defecation of the patients in the experimental group was shorter than that of the patients in the control group (t=-3.901,-6.689; P<0.01). Conclusions The individualized preoperative bowel preparation for patients with cervical cancer can improve the intestinal cleansing, shorten the time of defecation and reduce untoward effects such as abdominal distension and stomachache in these patients.

Objective To explore the effects of continuous urethral drainage in patients with long-term urethral catheter after radical operation for cervical cancer. Methods Totally 100 patients who received radical operation for cervical cancer in Wuxi Maternal and Child Health Hospital between January 2015 and December 2016, were selected and equally divided into the control group and the observation group, according to the random number table. The patients received indwelling urethral catheters post operation. Patients in the control group received convention nursing and the catheters were clipped during ambulation. The urethral catheters were removed on postoperative day 14 when the patients had to urinate after the functional recovery bladder was confirmed by a specially-assigned nurse. Patients in the observation group received continuous urethral drainage on the basis of conventional nursing. The indwelling urethral catheters were not clipped and were removed on day 14 postoperatively. First urine output, residual urine in bladders and replacement of urethral catheters were compared between the two groups. Results There was no significant difference between the two groups in first urine output, residual urine and replacement of urethral catheters (P＞ 0.05). Conclusions The continuous urethral drainage by indwelling catheters do not increase the incidence rate of uroschesis before they are removed in patients receiving radical operation for cervical cancer and is conducive to the functional recovery of bladders, which is worth promoting in clinical practice.

Objective To explore the effects of continuing nursing on shortening the time of wound healing and reducing the risk of wound infection following cervical loop eelectrosurgical excision procedure (LEEP). Methods A total of 100 patients receiving cervical LEEP in the Cervical Department of Wuxi Maternal and Child Health Hospital were selected by convenient sampling and divided into the control group (n=50) and the observation group (n=50) according to the time of admission. Patients in the control group received conventional nursing care, while patients in the observation group received continuing nursing on the basis of conventional nursing care as well as regular wound cleaning and dressing change for 6 weeks postoperatively. And multiple methods were used to enhance health education for them step by step. The rate of wound infection and the time of wound healing were compared between the two groups. Results After the intervention, there were statistical differences in the rate of wound infection and the time of wound healing between the two groups (P＜ 0.05). Conclusions Continuing nursing can reduce the rate of wound infection and shorten the time of wound healing following cervical LEEP, and enable the patients to return to society as soon as possible.

Objective:To explore effects of telephone transitional nursing after discharge from hospital on treatment effectiveness and quality of life of patients with lymphedema after breast cancer surgery.Methods:According to the convenient sampling method, a total of 85 patients with lymphedema after breast cancer surgery who were admitted to Department of Gynecology in Wuxi Maternal and Child Health Care Hospital from October 2017 to October 2019 were selected as research objects. According to the double-blind principle, the selected objects were divided into the study group ( n=43) and the control group ( n=42) . After discharge from hospital, patients in the study group were given telephone follow-up while patients in the control group were given routine follow-up. The effects of different methods on the quality of life and treatment effectiveness of patients with lymphedema after breast cancer surgery were compared. Results:After the follow-up, the difference in circumference of upper limbs of the study group was lower than that of the control group, and the difference was statistically significant ( P<0.01) . The anterior flexion and abduction of the shoulder joint of the study group were higher than those of the control group, and the differences were statistically significant ( P<0.01) . The total effective rate of treatment in the study group was higher than that in the control group, and the difference was statistically significant ( P<0.05) . After follow-up, except for role-physical, the quality of life scores in the study group were higher than those in the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The application of telephone transitional nursing after discharge from hospital in patients with lymphedema after breast cancer surgery can extend the nursing service, promote the recovery of shoulder joint function, ensure the effectiveness of treatment and improve the quality of life of patients, which is suitable for clinical promotion and application.