The traditional Chinese medicine (TCM) literature database of jaundice is an important means of the realization of the standardization and digitalization of TCM liver disease syndrome differentiation and treatment.
This article analyzed the background of jaundice literature database and its establishment and significance . The building of TCM jaundice database was described in this paper from literature data source, TCM jaundice the-saurus, literature collection and analysis. A brief introduction was made on contents of the database and the function. Deficiencies of the literature database such as the standardization, quality, daily maintenance, talent and team training were also explained .

Dental Bonding ; methods ; Dentin ; Dentin-Bonding Agents ; chemistry ; Electricity ; Humans

Adult ; Aged ; Aged, 80 and over ; Apoptosis Regulatory Proteins ; genetics ; metabolism ; Carcinoma, Non-Small-Cell Lung ; genetics ; metabolism ; Exons ; genetics ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Lung Diseases ; genetics ; metabolism ; Lung Neoplasms ; genetics ; metabolism ; Male ; Middle Aged ; Mutation ; Proto-Oncogene Proteins ; genetics ; metabolism ; RNA, Messenger ; metabolism

Background and Objective: Survivin has gradually become an important target in diagnosis,prognosis prediction and treatment of tumor.There are many studies on urine-based survivin mRNA test using reverse transcription-polymerase chain reaction(RT-PCR)as a noninvasive examination for bladder cancer.However,its clinical value remains controversial.This study was to evaluate the diagnostic value of urine survivin mRNA detection with RT-PCR for bladder cancer by a systematic review of related studies.Methods: With the search terms such as bladder neoplasm,survivin,RT-PCR,sensitivity,specificity and diagnosis,we systematically searched through PubMed,EMBASE,SCI,Cochrane Library.Chinese Biomedical Literature Database(CBM),Chinese Scientific Journal Full-Text Database(CSJD),China Journal Full-Text Database(CJFD),Chinese Medical Association(CMA)digital periodicals and Google Scholar totally from January 1997 to April 2009 for diagnostic trials with RT-PCR detection of urine survivin mRNA for bladder cancer.The Quality Assessment of Diagnostic Accuracy Studies(QUADAS)items were used to evaluate the quality of the included studies.Meta-disc software was used to calculate outcome indicators.Results: Twenty-six studies,totally 2416 patients,were eligible.Meta-analysis showed that compared with pathologic examination,the summary values of sensitivity,specificity,positive likelihood ratio,negative likelihood ratio and summary receiver operating characteristic curve(SROC)of urine-based survivin mRNA test using RT-PCR for bladder cancer were88%,94%,14.56,0.13 and 0.9736,respectively.Nested RT-PCR got the highest sensitivity,specificity and SROC and the values were 91%,95% and0.9805,respectively.The sensitivity and specificity of general RT-PCR were the second highest,which were 87% and 94%,respectively.The sensitivity of quantitative RT-PCR was 80% and the specificity was 93%.Conclusions:Comparing with pathologic examination,the sensitivity and specificity of urine-based survivin mRNA test using RT-PCR are relatively high.It can be used as an important adjunct method for cystoscope in early screening and postoperative monitoring of bladder cancer.

Objective To observe and analyze the effects of fetus gestational weeks on efficiency of Down's screening during the second trimester, and to provide certain reference data clinically. Methods A total of 36515 puerpera who received prenatal screening during the second trimester were selected. They were assigned to 5 groups according to their gestational weeks, which were group of 15 gestational weeks(15 to 16+6), group of 16 gestational weeks (16 to 16+6), group of 17 gestational weeks(17 to 17+6), group of 18 gestational weeks (18 to 18+6) and group of 19 ges-tational weeks (19 to 20 weeks). Detection rate and false screening positive rate of infants with Down's syndrome were compared between different gestation weeks. Results Two cases of missed diagnosis were seen in puerpera in the group of 15 gestation weeks. Detection rate of Down's screening in the group of 16 weeks to 18 weeks was 100%. No diagnosed infants with Down's syndrome were seen in the group of 19 gestational weeks. The false positive rate therefore was 7.85%. The screening results were different during different gestational weeks. Detection rate during 16 to 18 gestation weeks was 100%, and false positive rate of screening was 4.36%. In the negative groups of screened puerpera, AFP level increased with the increase of gestational weeks, and HCG level decreased with the increase of gestational weeks. In the positive groups of screened puerpera,their AFP levels were all lower than those in the nega-tive groups,and there HCG levels were higher than those in the negative groups. Conclusion Efficiency of screening is high when Down's screening during the second trimester of pregnancy is carried out during 16 to 18 gestational weeks.

OBJECTIVETo explore the relationship between latex allergen and clinical presentation as well as allergenic cross-reactivity between latex and other allergens, to know the incidence of latex allergy in Chinese children and elucidate the allergenic cross-reactivity of latex with other allergens.

METHODSTotally 265 children with allergic disorders were assayed with 13 international standard allergen agents by means of SPT.

RESULTSIn 79 children with latex allergenic SPT position, 53 were boys and 26 were girls with an average age of 5.6 years, and 14 cases had episodes occurred in winter, 14 cases in spring, 24 cases in summer, and 27 cases in autumn. Of them, 66 cases presented as asthma, 5 cases atopic skin disorders, 1 case anaphylactoid purpura, 1 case hives and 6 cases only had mild cough. Statistical analysis showed that the positive percentage of the latex SPT had no obvious relation with sex and age, but was higher in summers and autumns than in winters and springs (P < 0.01). Children with allergic symptoms had higher positive rate in latex allergenic SPT than those without them, that is, the positive percentage of the latex SPT significantly increased among children presenting with some allergic symptoms, such as asthma, hives and atopic skin disorders (P < 0.01). All the children with latex allergenic SPT position had cross-reactivity with acarid allergen, 62.0% approximately 43.0% with animal protein allergens including milk, cats, shrimp, dogs, eggs in the order of decreasing cross-reaction rate, and 10.1% - 3.8% with mold and plant farina allergens. But the cross-reactivity between latex and mold or tree farina I were not statistically significant.

CONCLUSIONThirty percent of the children with allergic disorders were latex allergenic SPT positive. Latex allergenic SPT positive results were significantly correlative to allergic clinical presentation and season, while were not relative to sex and age. The cross-reactivity of latex with acarid was most common, followed by animal protein allergens, while the cross-reactivity with mold and plant farina allergen was rare.

Allergens ; immunology ; Animals ; Asthma ; immunology ; Child ; Child, Preschool ; Cross Reactions ; Female ; Humans ; Infant ; Latex Hypersensitivity ; classification ; diagnosis ; immunology ; Male ; Predictive Value of Tests ; Seasons ; Skin Tests

OBJECTIVETo investigate the species, the distribution and the utilization of the medicinal plants from Selaginellaceae in Hubei Province.

METHODThrough field investigations and comparing the collected specimens and literatures, the classification and identification of the species in Hubei Province were studied.

RESULTThe results indicated that 15 species of plants from Selaginellaceae in Hubei, including 14 medicinal and 1 newly recorded species existed. The distribution and use in folk medicine were investigated. And the morphological description of several species was appended.

CONCLUSIONThe results provided a basis for the exploitation and utilization of the medicinal plant resources of Selagingellaceae.

China ; Conservation of Natural Resources ; Drugs, Chinese Herbal ; isolation & purification ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Plants, Medicinal ; anatomy & histology ; chemistry ; classification ; Selaginellaceae ; anatomy & histology ; chemistry ; classification

BACKGROUNDMyocyte apoptosis is considered to be the major causative factor of left ventricular (LV) remodeling following myocardial infarction (MI). We previously reported that 3', 4'-dihydroxyflavonol (DiOHF), was able to suppress oxidative stress and preserve the expression of endothelial nitric oxide synthase during myocardial reperfusion injury, which may benefit the reduction of myocyte apoptosis. We therefore aimed to evaluate the potential actions of DiOHF against myocyte apoptosis and post-infarction LV remodeling in this study.

METHODSFollowing experimental MI, surgical instrumented goats were randomly assigned into vehicle and DiOHF (2 mg/kg; i.v., daily) groups to receive 4 weeks of reperfusion with corresponding treatments. LV pressure recordings and echocardiogram were performed at baseline, 2 and 4 weeks of reperfusion. Myocardial tissues were collected in the end to determine infarct size and apoptosis related assays.

RESULTSLV end-diastolic volume and diameter were significantly increased 4 weeks after MI in the vehicle group, accompanied by reduced posterior wall thickness, septal thickness and LV mass, whereas those changes were markedly prevented by DiOHF treatment. Similarly, significantly reduced infarct size was found in DiOHF group as compared to vehicle group, and DiOHF dramatically inhibited the increase in LV end-diastolic pressure and the reductions in ejection fraction, fraction shortening and dP/dt(max). Moreover, DiOHF treatment significantly reduced the extent of myocyte apoptosis detected by TUNEL assay, enhanced the protein expression of caspase-3, Fas, Bax and cytochrome c in the non-infarcted myocardium in comparison to vehicle.

CONCLUSIONSDaily DiOHF treatment during the reperfusion period after MI in the ovine hearts markedly reduced the magnitude of post-infarction LV remodeling through the inhibition of myocyte apoptosis in the remote non-infarcted myocardium.

Animals ; Apoptosis ; drug effects ; Caspase 3 ; metabolism ; Cytochromes c ; metabolism ; Echocardiography ; Female ; Flavonols ; pharmacology ; Goats ; In Situ Nick-End Labeling ; Male ; Myocardial Infarction ; metabolism ; physiopathology ; Myocytes, Cardiac ; cytology ; drug effects ; Random Allocation ; Ventricular Remodeling ; drug effects ; bcl-2-Associated X Protein ; metabolism ; fas Receptor ; metabolism

OBJECTIVETo explore the possibility of repairing periodontal defects with carbonated calcium phosphate bone cement (CCPBC) modified with synthesized peptides.

METHODSPeriodontal bone defects in 4 dogs were surgically created and then restored directly with hydroxyapatite (HA), Perioglass, CCPBC and CCPBC modified with peptides. The results were compared at different levels.

RESULTSBone replacement materials were lost in HA and Perioglass groups. In the HA group defects were restored with connective tissue. Perioglass group had only a little new bone around materials by alveolar bone. CCPBC could firmly stay in bone defects to maintain the space of bone defects even without membrane use. CCPBC modified with peptides was superior to HA, Perioglass, and CCPBC, surrounded by a great deal of new bone.

CONCLUSIONUnder limitation of this study, CCPBC modified with peptides has some osteoinuctive activity and may have good prospect for the clinical application in periodontal defect repair.

Alveolar Bone Loss ; therapy ; Animals ; Bone Cements ; Bone Regeneration ; Bone Substitutes ; Calcium Phosphates ; Dogs ; Durapatite ; Male

Objective To explore the Electrocardiography (ECG) changes of residents in Keshan disease area and the status of growth and decline of Keshan disease in Shaanxi Province. Methods Using stratified randomized sampling method,2 mild,2 moderate and 2 serious disease counties were selected respectively in 2005 and 2006. A total of 6 counties were sampled,2 villages,one with severe disease and one with mild,were selected from each sampled county. A total of 12 villages were selected. The clinical examination and ECG were conducted in 3-year old children of agricultural population of the selected villages. Results ECG of 5692 cases were performed in the selected 12 village in the 6 counties,in which 4917 cases showed normal electrocardiogram,up to 86.38% (4917/5692). Two hundred and fifty-two cases showed roughly normal electrocardiograms,up to 4.43%(252/5692). Five hundred and twenty-three cases had abnormal electrocardiogram,accounting for 9.19% (523/5692). Among them,the abnormal electrocardiogram rates in mild,moderate and serious disease areas were 7.07% (144/2036), 11.41%(167/1646) and 10.54%(212/2010),respectively. Atrioventricular block was the major abnormal electrocardiogram change,followed by arrhythmia,ST-T changes,and low voltage. One hundred and thirty-nine cases were confirmed as latent and chronic Keshan diseases. One hundred and thirty-one cases were latent Keshan, and detection rate was 2.30%(131/5692). Eight cases were chronic Keshan,and the detective rate was 0.14% (8/5692). Complete right bundle branch block [37.06% (63/170) ],ST-T changes [22.35% (38/170) ],multiple premature ventricular beats [12.94% (22/170)] were the major abnormal electrocardiogram change of Keshan patients. Conclusions Atrioventricular block and arrhythmia are the major abnormal electrocardiogram changes. Keshan disease incidences are controlled under a stable condition.