Objective: To evaluate the protective effect of δ-opioid peptide, [D-Ala2-D Leu5]-enkephaline (DADLE), on pulmonary ischemia-reperfusion (I-R) injury after cardiopulmonary bypass (CPB), and to discuss the possible mechanism. Methods: Twenty-eight male pigs were evenly randomized divided into 4 groups, namely, the sham operation group, the CPB group (only subjected to CPB), the DADLE pretreatment group (received DADLE 1 mg · kg-1 before CPB), and the Glibenclamide group (received DADLE 1 mg · kg-1 and glibenclamide 1 mg · kg-1 before CPB). Peak inspiratory pressure (PIP), PaO2, and the plasma malondialdehyde (MDA) concentration were measured in each group before CPB and 10 min, 30 min, and 60 min after reperfusion. The changes of lung tissue wet weight/dry weight ratio (W/ D) were calculated before CPB and 60 min after reperfusion. The animals were sacrificed 60 min after reperfusion to observe the ultrastructural changes of the lungs and to quantitatively assess lung tissue damage (LTD). Results: There were no significant differences among the 4 groups before CPB and the parameters in sham-operation group had no changes at all specified time points. One hour after reperfusion, the PIP, MDA, W/D, and LTD values in Glibenclamide and CPB groups were significantly higher than those in sham-operation and DADLE groups while the PaO2 was significantly lower(P<0.05 or 0.01). There were no significant differences between parameters of sham-operation and DADLE groups except for LTD(P<0.05). There were no significant differences between parameters of Glibenclamide and CPB groups. Histological examination demonstrated that the degree of lung injuries in sham-operation and DADLE groups were slighter than those in Glibenclamide and CPB groups. Conclusion: It suggests that DADLE may exert its protective effect on the lung through opening ATP-sensitive K+ channels, so as to alleviate the lung I-R injury after CPB.

Humans ; Osteoporosis ; drug therapy ; Technetium Tc 99m Medronate ; therapeutic use

AIM: To study central corneal thickness ( CCT ) and correlation in different degrees of diabetic retinopathy ( DR) .
METHODS:A total of 65 cases (130 eyes) with different degrees of DR and 35 normal cases (70 eyes) as the age-and gender-matched control group were examined by corneal endothelial microscope, to measure CCT and statistics
RESULTS: Compared to control group, there were no significant difference of CCT both mild and medium nonproliferative diabetic retinopathy ( NPDR) groups ( P>0. 05 ). While the CCT of severe NPDR group and proliferative diabetic retinopathy ( PDR ) group were thicker than control group, and the differences were statistically significant (P<0. 05); Pairwise comparison in different degrees of DR groups: the CCT of severe NPDR and mild NPDR groups were thicker than PDR group, and the differences were statistically significant ( P<0. 05 );compared to medium NPDR, the CCT of PDR was thicker (P<0. 05);The thickness of CCT increases with severity of DR, there was positive linear correlation ( r=0. 173, P<0-05).
CONCLUSION: The CCT increases with severity of DR. Taking care of protecting corneal endothelium is very important in the time of therapeutic measure, especially intraocular operation, to decrease complication.

Humans ; Vascular Diseases ; surgery

Objective: To study the distributions of velocity, wall pressure and wall shear stress of blood flow in the human thoracic aorta under normal physiological pulsatile flow conditions, so as to provide a theoretical foundation for elucidating the pathogenesis of aortic dissection. Methods: By virtue of the Computational Fluid Dynamics and the fundamentals of hemodynamics, the authors obtained the numerical simulations and flow visualizations of pulsating blood flow in the human thoraric aorta. Results: The distributions of velocity, wall pressure, wall shear stress and path line of the blood flow in the thoracic aorta were calculated with a function of time in the cardiac circle. The systoltic wall pressure and its dp/dt were higher than diastolic wall pressure and its dp/dt. The pressure of lateral wall was higher than the pressure of medial wall during systole. A distinct depressed pressure was found located at the intersection between aortic arch and descending aorta. Shear stress of wall of lateral aortic arch was less than that of medial wall of aortic arch and the shear stress change in medial wall of aortic arch was greater than that in the lateral aortic wall. The shear stress was in a cyclic and alternative manner. Conclusion: The findings suggest that the wall pressure may be related to the development of aortic dissection.

Aged ; Chondrosarcoma ; Humans ; Laryngeal Neoplasms ; Male ; Middle Aged

Diagnosis, Differential ; Genital Neoplasms, Male ; metabolism ; pathology ; surgery ; Humans ; Male ; Middle Aged ; Neoplasms, Glandular and Epithelial ; metabolism ; pathology ; surgery ; Neprilysin ; metabolism ; Receptors, Estrogen ; metabolism ; Receptors, Progesterone ; metabolism ; Seminal Vesicles ; Stromal Cells ; pathology ; Vimentin ; metabolism

Objective: To investigate the chemical constituents of Isodon flavidus. Methods: All compounds were isolated and purified by silica gel, Sephadex LH-20, and MCI column chromatography. Their structures were elucidated by analysis on the physicochemical properties and spectral data. Results: Nine compounds were obtained from the Miao medicine I. flavidus and respectively elucidated as lophanic acid (1), isopimara-7,15-dien-19-oic acid (2), isopimara-7,15-dien-3β-ol (3), rubesanolide D (4), ursolic acid (5), β-sitosterol (6), oleanolic acid (7), 2α,3α-dihydroxy-12-ene-28-oic acid (8), and sesamin (9). Conclusion: Compounds 1-5 and 8-9 are isolated from this plant for the first time, compound 3 is isolated from the plants of Rabdosia (BL.) Hassk for the first time.

Objective Matrix metalloproteinase-10 (MMP-10) has been shown to be highly associated with atherosclerosis.Recent studies showed that levels of MMP-10 were elevated in infarcted tissues in acute ischemic stoke.However,serum levels of MMP-10 in patients with acute ischemic stroke have never been studied previously.This study aims to investigate the serum levels of MMP-10 in patients with acute ischemic stroke,and evaluate the association of serum levels of MMP-10 with stroke subtypes based on Trial of Org 10 172 in acute stroke treatment classifications,the severity of stroke,risk factors and carotid artery plaque.Methods The circulating levels of MMP-10 were measured by enzyme linked immunosorbent assay in 194 subjects,including 109 patients who were diagnosed as acute ischemic stroke in the Department of Neurology,Shengjing Hospital,China Medical University from April to December 2012,and the 85 healthy controls.Results Patients with acute ischemic stroke had higher serum levels of MMP-10 compared with healthy controls (6.59 (6.07,7.31) μg/L vs 5.16 (3.87,5.94) μg/L,Z =8.33,P ＜ 0.01).NIHSS score had positive correlation with serum levels of MMP-10 (r =0.204,P =0.037).Classified by risk factors,we compared the MMP-10 levels of subsets,and results displayed that statistically significant difference existed between dyslipidemia subset and non-dyslipidemia subset (Z =2.07,P =0.042).MMP-10 levels had positive correlation with serum levels of LDL-cholesterol (r =0.248,P =0.040),but negative correlation with thrombin-activatable fibrinolysis inhibitor (TAFI;r =-0.208,P =0.030).The subset with unstable plaques had higher MMP-10 levels than that with stable plaque (6.62 (6.13,7.36) μg/L) vs 6.10 (6.00,6.46) μg/L,Z =2.12,P =0.034),implying the relationship of MMP-10 and atherosclerosis.Conclusions Patients with acute ischemic stroke have higher serum levels of MMP-10 compared with the healthy controls,and MMP-10 levels have positive correlation with the severity of stroke.MMP-10 is associated with the subtypes of stroke classified by risk factors,and dyslipidemia subset has higher levels of MMP-10 than that of non-dyslipidemia subset.MMP-10 has positive correlation with LDL-cholesterol,but negative correlation with TAFI.MMP-10 may be involved in the process of formation and disruption of unstable plaques,which contribute to the stenosis of arteries and onset of acute ischemic stroke.

Adolescent ; Adult ; Carcinoma, Small Cell ; pathology ; therapy ; Female ; Humans ; Ovarian Neoplasms ; pathology ; therapy