95%).Strains of PNSP were significantly more common than those PSSP(72.56% vs 22.72%),and strains of Sp resistant to erythromycin were significantly more common than susceptible to erythromycin too(94.42% vs 0.88%).Conclusions The antimicrobial resistance of Sp from children with lower respiratory tract infection in Wenzhou area has became a severe problem.Multi-drug resistance of Sp has been observed to chlorodeoxylincomycin,erythromycin,tetracycline and so on.The results will provide some important information for the therapy and the choice of antibiotics of Sp infection.

OBJECTIVE: To study the genotype distribution of cytochrome CYP3A4, CYP2C9, CYP2C19, CYP2D6 in Han and Mongolian Chinese population and the allele and genotype frequency are compared between the Han and Mongolian. METHODS: CYP3A4, CYP2C9, CYP2C19, CYP2D6 of Han and Mongolian Chinese population were genotyped by PCR-RFLP. RESULTS: The allele frequencies of CYP3A4 * 5 in Han and Mongolian are 0, the allele frequencies of CYP3A4 * 18 in Han and Mongolian are 0.1838, 0.2025. The allele frequencies of CYP2C9 * 2 in Han and Mongolian are 0.0110, 0.0253; the allele frequencies of CYP2C9 * 13 in Han and Mongolian are 0, 0.003 2.The allele frequencies of CYP2C19 * 2 in Han and Mongolian are 0.386 0, 0.4146, the allele frequencies of CYP2C19 * 3 are 0.0515, 0.0443. The allele frequencies of CYP2D6 * 10 in Han and Mongolian are 0.5735, 0.4652. CONCLUSION: The study shows that there are no significant ethnic differences in the distribution of CYP3A4 * 18, CYP2C19 * 2, CYP2C19 * 3, CYP2D6 * 10 genotypes in Han and Mongolian. The CYP3A4 * 5 genotype is not found in this study and only one CYP2C9 * 1/* 13 genotype is found in Mongolian. For CYP2C9 * 2, the Mongolian is significantly lower than the Han, (P=0.023).

Animals ; Materia Medica ; pharmacology ; Medicine, Chinese Traditional ; Oligochaeta

Health Education ; Humans ; Occupational Health Services ; Rural Population

Objective In addition to myocardial ischemia,massive inflammatory mediators and different enzymes are released and free radicals increase during cardiopulmonary bypass(CPB) due to the contact of blood with foregin material Leukocytes play an important role The purpose of this study was to evaluate the myocardial protective effect of leukocyte depleted blood perfusion during open heart surgery Methods Thirty adult ASA II IV patients scheduled for elective orthotopic valve replacement received fentanyl enflurane anesthesia A bolus of 3 mg/kg intravenous heparin was given before CPB Cardiac arrest was induced with 4 ℃ hyperkalemic crystalliod cardioplegic solution Patients were randomly allocated to one of 3 groups based on the types of cardioplegic solution used during CPB: crystalloid solution(group CS, n=10),whole blood (group WB,n=10), and leukocyte depleted blood (group LD,n=10) Blood samples were taken from peripheral artery before heparinization, 5 min after initiation of CPB ,5 min before and 30 min, 1 h, 2 h and 24 h after declamping of aorta respectively for determinations of creatine kinase MB(CK MB), interleukin 8(IL 8) and tumor necrosis factor alpha(TNF?) Myocardial tissuses were obtained from right atrium before cross clamping of aorta, before and 15 min after declamping of aorta for determinations of water content,Ca 2+ content and ultrastructure examination of myocardium Results After declamping plasma concentrations of CK MB and IL 8 significantly increased in all groups as compared to the values before declamping (P

Background Many studies showed that attenuation of retinal nerve fiber layer(RNFL)in early glaucoma is one of the important signs.How to accurately and quantitatively measure RNFL thickness is very important for the early diagnosis and monitoring of glaucoma.0bjective This study was to evaluate the clinical value of Optovue RTVue OCT and Heidelberg Retina Tomograph Ⅲ(HRT-Ⅲ)confocal scanning laser ophthalmoscopy in glaucomatous eyes. Methods This cross-sectional study included 40 eyes of 26 patients with suspected open-angle glaucoma(SOAG),48 eyes of 29 patients with open-angle glaucoma and 48 eyes of 27 healthy subjects.Optical nerve head(ONH) parameters and peripapillary RNFL thickness were measured in all the subjects with Optovue RTVue OCT and HRT-Ⅲ,meanwhile all the eyes received perimetry with Humphrey 750-I.Glaucoma variables obtained from Optovue RTVue OCT and HRT-Ⅲ were analyzed among the groups.Topographic Optovue RTVue OCT and HRT-Ⅲ parameters,including disc area(DA),cup area(CA),rim area(RA),rim volume(RV),cup volume(CV),cup/disc area ratio(C/DAR)as well as superior,temporal,inferior and nasal average RNFL thickness,were analyzed.The relationship of ONH parameters and RNFL thickness was analyzed using a linear correlation.The correlation between the mean defect(MD)of the visual field and the tomography parameters in glaucomatous eyes was described by bivariate Pearson correlation coefficients.Resuits The ONH parameters and RNFL thickness obtained by HRT-Ⅲ and OCT showed significant difference(P<0.05).ONH parameters such as RA,C/DAR,CA and RV were statistically changed in SOAG and the POAG patients compared with the normal subjects(q=6.47,q=7.67,P<0.05).The superior and inferior RNFL thickness parameters in three groups were positively correlated between HRT-Ⅲand OCT(r=0.362,r=0.441,r=0.395,P<0.05),Topographic Optovue RTVue OCT and HRT-Ⅲ parameters including CV,CA,RA and C/DAR fitted Pearson analysis(all P<0.05).In POAG group。The RA,RV,CV,C/DAR from Optovue RTVue OCT were correlated with MD with the significant coefficient 0.284,0.286,0.340,0.371 respectively(P<0.05),and evidently correlations also were found between RA,RV,C/DAR with MD respectively with the coefficient 0.339,0.859,0.422(P<0.05)by HRT-Ⅲ. Conclusion Both Optovue RTVue OCT and HRT-Ⅲ can difierentiate ONH analysis with a similar outcome in glaucomatous eye.The C/D value.RA,superior and inferior RNFL thickness based on Optovue RTVue OCT and HRT-Ⅲare distinguishing indexes in the diagnosis of early glaucomatous damage.

Objective To study the mechanisms of Liujing Toutong Tablets (LTT) in the treatment of migraine by network pharmacology. Methods Eighteen components of LTT absorbed into blood were selected as the objects, and targets of compounds were predicted according to the reverse pharmacophore matching method. The related pathways and correlation analysis were explored by the MAS 3.0 biomolecule function software and the Kyoto Encyclopedia and Genomes (KEGG) database. Finally, the network of compound-target-pathway-disease of LTT was constructed by Cytoscape software. Results Eighteen compounds interacted with 88 pathways through 72 targets, of which 36 protein targets and 22 pathways were associated with migraine. Conclusion LTT may play a role in expelling wind and dredging collaterals, promoting orifice and pain-relieving by intervening with the related pathways of inflammation, homeostasis of blood vessels, nervous centralis, immunity, and hormonal regulation.

Objective: To screen for the genes associated with metastasis of head and neck squamous cell carcinoma (HNSCC) by studying the specimens of HNSCC. Methods: Eighty HNSCC specimens, including 17 involving the tongue, 13 involving mouth, 14 involving oropharynx, and 36 involving hypopharynx, were subjected to single nucleotide polymorphism chip analysis. The thirty-nine patients with distant metastasis during follow-up were taken as experimental group and the 41 without distant metastasis were taken as controls. Cox regression analysis was used to examine the influence of single nucleotide polymorphism (SNP) on distant metastasis of HNSCC. Results: The results of SNP demonstrated a distinct peak of frequent genomic gain at 11q13. Cox analysis of the array data showed the following results: a relative risk of T allele of rs965l738 as the SNP probe of SHANK2 gene was 3.61(P = 0.003), relative risk of T allele of rs4980690 as the SNP probe of FGF4 gene was 3.63(P = 0.006), relative risk of T allele of rs7929885 as the SNP probe of ANO1 gene was 4.35(P = 0.008), relative risk of T allele of rs687660 as the SNP probe of PPFIA1 gene was 4.24(P=0.011), relative risk of T allele of rs660665l as the SNP probe of ORAOV1 gene was 3.04(P = 0.013), and relative risk of T allele of rs592412 as the SNP probe of CCND1 gene was 4.07(P = 0.013). Conclusion: It is indicated that the following genes at 11q13 can increase the risk of metastasis of HNSCC, including T allele of rs965l738 in SHANK2, T allele of rs4980690 in FGF4, T allele of rs7929885 in ANO1, T allele of rs687660 in PPFIA1, T allele of rs6606651 in ORAOV1, and T allele of rs592412 in CCND1.

Objective To identify risk and protective factors of the periventricular-intraventricular hemorrhage (PV-IVH) in preterm infants. Methods By 1:1 case-control study, prenatal and perinatal data were collected and analyzed between preterm infants with PV-IVH and control group from January 2012 to October 2014. The risk and protective factors for the PV-IVH were identiifed by univariate analysis and multivariate conditional logistic regression analysis. Results There were one hundred and thirty-two preterm infants diagnosed of PV-IVH, in which, among whom 6 preterm infants could not be matched to the control infants in the protocol. Finally, 126 pairs of infants were enrolled in the study. There were no differences between two groups in gestational age and birth weight (all P>0.05). Multivariate conditional logistic regression analysis found that BE<-5 mmol/L in the initial blood gas analysis after birth (OR=1.986, 95.0%CI:1.039-3.796), mechanical ventilation (OR=2.913, 95%CI:1.390-6.101), weight gain≤10 g/d in the second week (OR=2.303, 95%CI:1.164-4.558) were risk factors, while number of previous pregnancies≥1 times (OR=0.426, 95%CI:0.229-0.792) was a protective factor for PV-IVH. Conclusions The risk factors of PV-IVH in preterm infants include the lower BE value in the initial blood gas analysis, required mechanical ventilation, and less weight gain in the second week.

Mixed-ownership hospitals constitute an experiment of such ownership in the health care sector,attracting high attention as of its birth.This paper introduced the background of such ownership,and analyzed the development of such hospitals in terms of policy,present situation and external effects.The authors,arguing against major challenges,stated their views on such hospitals along with in-depth analysis of key issues in their development.