OBJECTIVE: To investigate the effect of antacids on the efficacy and safety of sucralfate. METHODS: The appearance of sucralfate suspension gel was observed under different pHs in vitro to learn the appropriate pH for sucralfate to work. The adverse effects of sucralfate was evaluated by determining the aluminum ion concentration in the supernatant using emission spectrometry with inductively coupled plasma (ICP-OES). RESULTS: At pH 0.1, sucralfate was partly dissolved; at pH 1.0 and 2.0, it formed white soft gel with strong adhesion and good extension; along with the storage time, the gel gradually precipitated; at pH≥3, sucralfate was unable to form gel; at pH >4.0, it formed a hard white precipitate without obvious adhesion and spreadability. In the pH range of 0.1-7.0, the concentration of free aluminum ion reduced gradually with the increase of pH. CONCLUSION: Combination use of antacids with sucralfate does not increase the aluminum ion absorption, but whether there is a synergistic effec is worth of further exploration.

To obtain high-yield avilamycin-producing strains,low energy N~+ ion implantation technology and screening of streptomycin-re- sistant mutants are used in the study on breeding mutation.The results show that,“saddle”region,which range is from 3?10~(15) to 5?10~(15) ions/cm~2,has got better induced mutation action.It also means that the strain's resistant mutation and yield mutation closely correlate to each other,and the method of streptomycin resistant screening is feasible.We have isolated a high-yield strain SVT-45 which the productivi- ty is 195% higher than the original strain's in the rotation-flask experiments.These results showed that the ion implantation was an effective method for microbe mutagensis.

To study the chemical constituents from the bark of Myrica rubra, fourteen compounds were isolated from the methanolic extract using various chromatographic techniques, including silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified on the basis of chemical properties and spectroscopic data, as 3, 5-dimethoxy-4-hydroxymyricanol (1), myricanol (2), myricanone (3), myricanol 11-sulfate (4), myricitrin (5), quercetin (6), quercetin-3-rhamnoside (7), tamarixol (8), uvaol (9), ursolic acid (10), taraxerol (11), myricadiol (12), β-sitosterol (13) and β-daucosterol (14). Among them, compound 1 is a new compound, named as 3, 5-dimethoxy-4-hydroxymyricanol, compounds 8, 9 were isolated from the genus Myrica for the first time.
Diarylheptanoids ; chemistry ; isolation & purification ; Myrica ; chemistry ; Phytochemicals ; chemistry ; isolation & purification ; Plant Bark ; chemistry

Small interfering RNA (siRNA) is the initiator of RNA interference and inhibits gene expression by targeted degradation of specific messenger RNA. siRNA-mediated gene regulation has high efficiency and specificity and exhibits great significance in the treatment of diseases. However, the naked or unmodified siRNA has poor stability, easy to degrade by nuclease, short half-life, and low intracellular delivery. As an emerging non-viral nucleic acid delivery system, ionizable lipid nanoparticles play an important role in improving the druggability of siRNA. At present, one siRNA drug based on ionizable lipid nanoparticles has been approved for the treatment of rare disease. This review introduces the research progress in ionizable lipid nanoparticles for siRNA delivery, focusing on the effect of each component of lipid nanoparticles on the efficiency of siRNA-mediated gene silencing, which provides new references for the studies on ionizable lipid nanocarriers for siRNA delivery.

Objective: To explore the influence factors of processing Aconiti Lateralis Radix Praeparata by microwave-drying, so as to lay a foundation for its industrial development. Methods: The drying process of Aconiti Lateralis Radix Praeparata was investigated by water ratio, drying rate and energy consumption analysis. The aconite morphology, contents of diester alkaloids, monoester alkaloids and alkanolamine alkaloids were used as indexes to study the effects of slice thickness, water content, microwave power and processing time on aconite processing. Results : The thickness, rehydration rate, microwave power and processing time had influence on the processing process and alkaloid content of aconite. When the thickness was 5 mm, the drying process had shorter drying time, faster drying rate and lower the energy consumption, but it had a higher content of diester alkaloids and lower content of the total alkaloids. When the rehydration rate of raw aconite slices was 60%, the drying time was the shortest. The order of average drying rate was 100% rehydration rate > 60% rehydration rate > 80% rehydration rate > 40% rehydration rate. The order of content of diester alkaloids was 60% rehydration rate > 100% rehydration rate > 80% rehydration rate > 40% rehydration rate. The order of total alkaloids content was 60% rehydration rate > 80% rehydration rate = 100% rehydration rate > 40% rehydration rate. The influence of rehydration rate on energy consumption was not obvious. When the processing time was the same, the drying process under the microwave power of 550 W had the shortest drying time, the fastest drying rate, the lowest content of diester aconitine and the highest content of total alkaloid. However, with the increase of processing time, the aconite was easier to be burnt under a high microwave power. Conclusion: The appropriate thickness, moisture content, microwave power and processing time in aconite processing process are helpful to reduce the toxicity of aconite and improve the quality of aconite. Therefore, the thickness of the slices should choose 3 mm, the moisture content should be 60%-80%, and the processing time should be reduced according to the increase of microwave power.

OBJECTIVETo predict part of medicinal properties of traditional Chinese medicine components and traditional Chinese medicine decoction pieces by using the traditional Chinese medicinal property data prediction platform, in order to establish the relationship between properties of traditional Chinese medicine components and traditional Chinese medicine decoction pieces.

METHODThe properties of traditional Chinese medicine components were predicted by using the medicinal property data prediction platform based on the pharmacological effects of the components.

RESULTThe total sum of identical or similar results of the prediction for the properties of traditional Chinese medicine components and traditional Chinese medicine decoction pieces accounted for over 75%.

CONCLUSIONThe self-similarity exists between properties of traditional Chinese medicine components and traditional Chinese medicine decoction pieces, which reflects the inheritance, additivity and emergence among different properties of traditional Chinese medicines.

Drug Combinations ; Drug Prescriptions ; Drug Therapy ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Medicine, Chinese Traditional

Objective To evaluate the effect of Metapex for root canal therapy of deciduous teeth. Methods Based on the key words"Metapex paste" and"Root canal therapy of deciduous teeth" , the Chinese database, such as China national knowledge infrastructure, Wanfang database and Weipu database were retrieved. Based on the key words"Metapex" and"deciduous teeth" , the English databases such as PubMed and Medline were retrieved. Collecting random controlled trials about Metapex paste with root canal therapy of deciduous teeth from the beginning of their establishment to August 2017. Do Meta analysis using Rev Man 5.3 software for homogeneous studies, calculating risk ratio (RR) and 95％ confidence interval (CI) . Results Twelve studies were included. Meta analysis showed that Metapex was more effective in root canal therapy of deciduous teeth compared with zinc oxide eugenol iodoform paste after 1 year (RR=1.11, 95％ CI: 1.01-1.22); The clinical effectiveness of Metapex paste was better than calcium hydroxide after 6 months and 1 year (RR6 mouths=1.06, 95％ CI: 1.03-1.09; RR1 year=0.17, 95％ CI: 1.03-1.10); There was no obvious difference between Metapex and ZOE after 6 months (RR=1.13, 95％ CI:1.00-1.27) . Conclusion Metapex paste can improve the success rate on root canal therapy of deciduous teeth , is a more ideal root canal filling material of deciduous teeth .

Objective To investigate the expression and variation of MIP 1β,MIP-2,and IL-12p70 in mice with bloodstream infection caused by 4 kinds of bacteria.Methods CD-1 (ICR) mouse models of bloodstream infection with Staphylococcus aureus (S.aureus),Enterococcus f aecalis (E.f aecalis),Escherichia coli (E.coli),and K lebsiella pneumoniae (K.pneumoniae) were established.After mice in each trial group and PBS control group were infected by bacteria for 0.5h,1h,3h,6h,12h,24h,and 48h,concentrations of MIP-1β,MIP-2,and IL-12p70 were detected by Luminex liquid suspension chip system.Results Concentrations of MIP-1β increased significantly 1h after bacteria was in blood,S.aureus,E.faecalis,E.coli,K.pneumoniae,and control groups were (134.5 ± 18.3),(61.5 ± 15.4),(3 354.0 ±809.0),(6 888.4 ± 1 100.2),and (28.9 ± 4.6) pg/mL respectively;the peak values of IL-12p70 were (389.3 ± 118.1),(127.6 ± 10.0),(42.2 ± 3.5),(62.8 ± 8.4),and (4.8 ± 0.3) pg/mL respectively.Concentrations of MIP-1β and MIP-2 in E.coli and K.pneumoniae groups were significantly higher than other trial groups and control group (all P＜0.01),while concentrations of IL-12p70 in S.aureus and E.faecalis groups were both significantly higher than E.coli,K.pneumoniae,and control groups (all P＜0.01).Conclusion Concentrations of MIP-1β and MIP-2 in E.coli and K.pneumoniae groups were both significantly higher than those in S.aureus and E.faecalis groups,while concentrations of IL-12p70 in S.aureus and E.faecalis groups were both significantly higher than those in E.coli and K.pneumoniae groups.The combination detection of multiple cytokines or chemokines are valuable in predicting gram-positive or gram-negative bacterial infection,and can provide basis for treatment of early infection.

Objective:To evaluate the effect of transcutaneous electrical acupoint stimulation (TEAS) on the sleep quality after cesarean section and postpartum depression in parturients with sleep disorder.Methods:A total of 225 parturients, aged 20-45 yr, weighing 50-80 kg, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ, scheduled for elective cesarean section, were enrolled in this study.The Pittsburgh Sleep Quality Index (PSQI) scale was used to investigate the sleep quality before operation.PSQI score<7 was defined as having no sleep disorder, and the patients were included in the non-sleep disorder group (NSD group, n=76). PSQI score≥7 was defined as having sleep disorder, and the patients were randomly divided into sleep disorder group (SD group, n=73) and TEAS group ( n=76). Routine combined spinal-epidural anesthesia was performed in NSD group and SD group.In TEAS group, Neiguan, Baihui, Zusanli and Sanyinjiao acupoints were selected for performing TEAS from the completion of combined spinal-epidural anesthesia to the end of operation, and TEAS was performed for 30 min on the 1st and 2nd days after operation.Patient-controlled intravenous analgesia was applied after operation in the three groups.The blood samples were taken from the cubital vein before operation and at 1 and 2 days after operation for determination of the plasma melatonin and prolactin concentrations (by enzyme-linked immunosorbent assay). The Leeds Sleep Evaluation Questionnaire (LSEQ) was used to evaluate the quality of sleep before operation and at 1, 2 and 7 days after operation.The Edinburgh Postpartum Depression Scale was used to evaluate the postpartum depression at 6 weeks after operation. Results:Compared with NSD group, LSEQ scores were significantly decreased at each time point after operation, the incidence of postpartum depression was increased, and the concentrations of plasma melatonin and prolactin were decreased, and the lactation initiation time was prolonged in SD group ( P<0.05). Compared with SD group, LSEQ scores were significantly increased at each time point after operation, the incidence of postpartum depression was decreased, and the concentrations of plasma melatonin and prolactin were increased, and the lactation initiation time was shortened in TEAS group ( P<0.05). Conclusion:TEAS can raise the the sleep quality after cesarean section and reduce the occurrence of postpartum depression in the parturients with sleep disorders.

OBJECTIVETo investigate the effect of laparoscopic-assisted resection of rectal carcinoma on perioperative levels of C-reactive protein (CRP), immunoglobulins(Ig) and complements.

METHODSFifty-four patients with rectal carcinoma were divided into laparoscopic group (n=26) and open operation group(n=28) according to the patients' will. C-reactive protein (CRP), IgG, IgA, IgM, C(3), C(4) and CH(50) of peripheral blood were assayed preoperatively and on the 1st, 2nd, 3rd and 7th day postoperatively.

RESULTSCompared with the preoperative period, the CRP levels in both groups were significantly increased on the 1st, 2nd, and 3rd day(P<0.01) and peaked on the 2nd day postoperatively. The postoperative CRP levels were significantly lower in the laparoscopic group than those in the open operation group(P<0.01). After operation, the immunoglobulin levels were significantly decreased in both groups(P<0.01), and there were no significant differences in IgG and IgM. The levels of C(3), C(4) and CH(50) were significantly decreased after operation in both groups(P<0.05) and returned to the preoperative levels during postoperative 48-72 hours in laparoscopic group.

CONCLUSIONLaparoscopic-assisted resection of rectal carcinoma results in less wound, lower levels of stress response, and less effect on immune function compared to open surgery, which recovers the immune function of patients more rapidly after operation.

C-Reactive Protein ; metabolism ; Female ; Humans ; Immunity, Humoral ; Laparoscopy ; Male ; Middle Aged ; Rectal Neoplasms ; blood ; immunology ; surgery