Objective To discuss the influence of two recombinant hemoglobin (rHb1.1 and rHb2.0) and human serum albumin (HSA) on oxygen supply and demand balance in rat with coronary heart disease (CHD). Methods Male Wistar rats were randomly divided into normal control group, CHD model group, HSA treatment group, rHb1.1 treatment group and rHb2.0 treatment group, 20 rats in each group. Rat model of CHD was established by high fat diet combined with pituitrin injection. The mean arterial pressure (MAP) decreased to 40 mmHg (1 mmHg = 0.133 kPa) after femoral arterial blood was drawn from the femoral arteries, and the rats were resuscitated with 13.4% HSA, rHb1.1 and rHb2.0, respectively, at the rate of 60 mL·kg-1·h-1 (20 mL/kg). The changes of electrocardiogram (ECG) ST-segment were calculated before model reproduction and at 12 hours after the last time injection of pituitrin. MAP, heart rate (HR), superior mesenteric artery blood flow (QSMA) and arterial blood gas analysis were recorded at 0, 30, 60, 90 and 120 minutes after the administration. The blood was collected after 12-hour fasting, and serum total cholesterol (TC) and triglyceride (TG) were determined by enzymatic method. The pathological changes in cardiac tissue were observed with light microscope. Results Compared with the normal control group, the changes of ECG ST-segment and TC, TG of model group were significantly increased. Compared with the model group, rHb can significantly reduce the value of ST segment changes, and HSA has no such effect; rHb short-term infusion has no significant effect on blood lipids, but can reduce myocardial pathological changes. Compared with the normal control group, the MAP of the model group decreased significantly, the HR was increased, the QSMA was slowed down, the pH value, the residual alkali (BE), the arterial carbon dioxide partial pressure (PaCO2) and HCO3- were decreased significantly. MAP in rHb1.1 group and rHb2.0 group were significantly higher than those in HSA group. Values of MAP were significantly higher in rHb2.0 group than those in rHb1.1 group at 90 minutes and 120 minutes (mmHg: 80.9±3.3 vs. 69.4±4.9, 79.2±4.0 vs. 69.1±3.7, both P < 0.05). The HR of HSA, rHb1.1 and rHb2.0 decreased to normal in 30 minutes after administration, significantly lower than those in the model group (bpm: 534±46, 518±28, 526±37 vs. 609±52, all P < 0.05). In the rHb2.0 group, the QSMA increased significantly at 60, 90 and 120 minutes compared with the model group (qv·mL-1·min-1: 5.6±0.4 vs. 3.9±0.6, 6.2±0.6 vs. 4.1±0.4, 6.9±0.7 vs. 4.0±0.3, all P < 0.05), but there was no significant difference between the HSA group and the rHb1.1 group. The pH, BE, PaCO2 did not return to the normal level after administration of HSA; pH, PaCO2 and HCO3- in the rHb1.1 group returned to normal level at 60 minutes after administration, and BE returned to normal level at 90 minutes after administration. Each index in rHb2.0 group can restore to normal levels 30 minutes ahead of. Conclusion Recombinant hemoglobin can significantly improve the oxygen supply and demand balance of rats with CHD model, can quickly and effectively correct the hypoxic state of blood metabolic acidosis, and rHb2.0 has better effect than rHb1.1.

Objective To investigate the expression of marrow-derived thyroid stimulating hormone β (TSHβ) splice variant in thyroid of mouse with autoimmune thyroiditis induced by thyroglobulin(Tg) immunization,and to analyze whether TSHβ splice variant participated in the pathological process of autoimmune thyroiditis.Methods Using random number table,forty-eight mice(24 females and 24 males) of 7 to 8 weeks old with body mass 20 to 25 g were randomly divided into 4 groups(12 females and 12 males in each group) based on body weight and gender.The control group:fed with deionized water; the Tg-treated group(TG):fed with deionized water,and immunized subcutaneously with 0.1 mg Tg at 8 weeks old,boost immunized twice at 11 and 15 weeks old,respectively; the high iodine-treated group (HI):fed with deionized water containing 0.05％ sodium iodide (NaI); the Tg combined with HI group (TG + HI):fed with deionized water containing 0.05％ NaI,and immunized the same way as the TG group did.Peripheral blood was collected after 8 weeks treatment,which was used for determination of total tetraiodothyronine (TT4),free tetraiothyronine (FT4),total triiodothyronine (TT3) and free triiodothyronine (FT3) with chemiluminescence immunoassay (CIA); thyroid glands were collected to examine the expression of TSHβ splice variant with SYBR Green fluorescent quantitative real-time PCR,and frozen sections were HE stained for observation of histopathological changes of thyroid cells under light microscopy.Results Under naked eyes,the thyroid gland enlarged significantly,and looked dark red in HI and TG + HI groups.Under an optical microscope,thyroid follicular epithelial cells presented cuboidal,with abundant cytoplasm,presented abundant glial in follicular cavity,without lymphocyte infiltration in the control group; in TG group,the thyroid follicular epithelial cells presented cuboidal,with some single scattered lymphocytes; in HI group,colloid volume expansion appeared in thyroid follicles,thyroid follicular epithelial cells presented low cuboidal or flat,with few single scattered lymphocytes; in TG + HI group,most colloid accumulative large follicles presented in thyroid,thyroid follicular epithelial cells presented flat,some destructive thyroid follicular structure and infiltrating lymphocytes appeared.The differences of FT3,TT4,FT4 and TSHβ splice variant between groups were statistically significant(F =4.00,12.54,31.92,214.29,all P ＜ 0.05).Compared to the control group,the serum TT3(nmol/L:0.92 ± 0.07 vs.1.30 ± 0.33,t =-2.24),TT4(nmol/L:1.30 ± 0.33 vs.95.60 ± 14.10,t =-3.02),FT4(pmoL/L:54.07 ± 3.67 vs.154.80 ± 0.01,t =-54.87) and the thyroids' TSHβ(× 10-3:4.11±0.32 vs.8.38 ± 0.22,t =-19.11) were higher in TG group(all P ＜ 0.05) ; the serum TT4(nmol/L:67.75 ± 11.91 vs.45.50± 3.85,t =3.55,P ＜ 0.05) was lower in HI group; the serum FT4(pmol/L:54.07 ± 3.67 vs.139.46 ± 30.00,t =-5.65) and the thyroids' TSHβ splice variant (× 10-3:4.11 ± 0.32 vs.5.33 ± 1.47,t =-5.95) were higher in TG + HI group (all P ＜ 0.05).Conclusions High iodine has aggravated thyroiditis of BALB/c mice induced by Tg immunization; the level of thyroid TSHβ in mice with autoimmune thyroiditis is higher; all of these results indicated that TSHβ is involved in the pathogenesis of autoimmune thyroiditis.

Infectious bursal disease virus (IBDV) VP4 plays an important role in immunosuppression of host. In order to develop monoclonal antibodies (McAbs) against VP4, we vaccinated BALB/c mice with His-VP4, screened and subcloned positive clones. We established 4 hybridoma cell lines that stably secreted McAbs against VP4 and named these cell lines 3B3, 3H11, 4C8 and 4G6, respectively. We tested the dissociation constant (Kd) of these McAbs, and found that their K(d)s were 4.61 x 10(-11), 1.71 x 10(-10), 4.26 x 10(-11), 5.02 x 10(-11), respectively. The isotypes of these McAbs were determined to be IgG1, IgG1, IgG2b and IgG1. These McAbs specifically bound to VP4 in IBDV infected DF-1 cells as demonstrated by Western blotting analysis and fluorescence antibody assay. These McAbs would help to detect IBDV infection and to analyze the biological activities of IBDV VP4.
Animals ; Antibodies, Monoclonal ; biosynthesis ; Blotting, Western ; Cell Line ; Fluorescent Antibody Technique ; Hybridomas ; Infectious bursal disease virus ; Mice ; Mice, Inbred BALB C ; Viral Structural Proteins ; immunology

Objective To estimate the safety and efficacy of submucosal tunneling endoscopic resection (STER) on treatment of large esophageal submucosal tumors (SMTs) originating from muscularis propria layer.Methods The data of patients with large esophageal SMTs (diameter ≥ 3.5 cm) undergone STER (n=17) or endoscopic submucosal dissection (ESD,n =15) at the Endoscopy Center of Tianjin Medical University General Hospital from December 2009 to March 2016 were retrospective analyzed.The therapeutic effects,hospitalization times,post-operation expenses,and occurrence of complications were evaluated and compared between the two groups.Results All the endoscopic treatments of the 32 patients were successfully completed.The operating time of the STER group was significantly longer than that of the ESD group (t =2.595,P =0.015).There was no statistical difference on the en bloc resection rate,complete resection rate and complication rate between STER group and ESD group (P＞0.05).The mean post-operative hospital stay of the STER group was significantly less than that of the ESD group (3.8± 1.0days VS 6.7±1.8 days,t=5.644,P=0.000).The mean hospital cost of the STER group was significantly less than that of the ESD group (22 456.1±5 232.0 yuan VS 27 392.5±5 747.9 yuan,t =2.543,P =0.016).The wound healing rates at 1 month after operation in the STER group was significantly higher than that of the ESD group [94.1％ (16/17) VS 20.0％ (3/15),P=0.000].No recurrence and metastasis occurred in the STER group and ESD group during the 41.2±20.6 months follow-up.Conclusion STER is a safe and effective technique for treating large esophageal SMTs originating from the muscularis propria layer,with earlier wound healing,shorter hospital stay and lower cost compared with those of the traditional method of ESD.

Lung cancer is one of the most common malignancies. Anti-tumor drugs with intravenous administration have systemic adverse effects as well as limited efficacy. Drugs can concentrate in lungs after pulmonary administration,which limits the distribution in the other organs and reduces the side effects of anti-tumor drugs. The paper focused on the recent progress in the studies on new dos-age forms of anti-tumor drugs for pulmonary administration for the therapy of lung cancer,so as to provide reference for the development of anti-tumor drugs for pulmonary administration.

Objective:To explore the moderating effect of associative stigma on the relationship between personality and professional identity among students majoring in psychiatry.Methods:The Chinese version of the 10-item Big Five Personality Inventory(TIPI-C10),the Chinese version of the Clinician Associative Stigma Scale(CASS-C),and the College Students'Professional Identity Questionnaire were used to investigate the personality traits,levels of associative stigma,and levels of professional identity among 342 psychiatric students.The main statistical analyses used were correlation analysis and moderation effect testing.Results:When the level of associative stigma was high,extraversion positively predicted professional identity(simple slope=1.047,t=3.018,P<0.01).When the level of associative stigma was low,extraversion positively predicted professional identity(simple slope=2.094,t=6.091,P<0.001).When the level of associative stigma was high,conscientiousness positively predicted professional identity(simple slope=1.781,t=3.986,P<0.001);When the level of associative stigma was low,conscientiousness positively predicted professional identity(simple slope=3.684,t=8.681,P<0.001).When the level of associative stigma was high,emotional stability positively predicted professional identity(simple slope=0.965,t=2.388,P<0.05).When the level of associative stigma was low,emotional stability positively predicted professional identity(simple slope=2.998,t=7.175,P<0.001).Conclusion:Associative stigma played the moderating effect between extraversion,conscientiousness,emotional stability,and the professional identity of psychiatric students.Colleges and universities should carry out targeted education and publicity work based on the personality traits of different students.

Objective To establish a quality control method for bomeol and artificial musk in Xinfufang-Zhenzhusan and Xinfufang-Zhenzhugao.Methods We used petroleum ether-toluene-ethyl acetate (9:3:2)as developer for TLC to identify isoborneol and borneol and petroleum ether-dichloromethane (2:3) as developer for TLC to identificate musk ketone.Agilent 7890 B gas chromatograph,FDI detector;Column:Thermo-TG-WaxMS GC (0.25 mm × 30 m,0.25 mm) was employed;the carrier gas was high purity nitrogen and flow rate for 1 mg/ml,the injection port temperature is 200 C and detector temperature is 250 ℃;the split ratio is 10:1 and injection volume was 1 μl,using temperature programmed.Results The isoborneol,borneol and musk ketone in the range of 0.001-10 mg/ml showed good linearity.The recovery of the method is in the range of 95 ％ to 105 ％.The TLC for isobomeol,bomeol,musk ketone can be identified easily.Conclusions The method was simple and reasonable,which can be used for the quality control of borneol and artificial musk in the Xinfufang-Zhenzhusan and Xinfufang-Zhenzhugao.

Objective To investigate the relationships of serum levels of human fractalkine(CX3CL1)and chitinase-3-like protein 1(YKL-40)with early cognitive impairment in elderly patients with Alzheimer's disease(AD).Methods A total of 110 AD patients in the Second Affiliated Hospital of Xinxiang Medical University from February 2021 to December 2023 were selected as AD group,and 50 healthy individuals with physical examination during the same period were selected as control group.Clinical materials and serum levels of CX3CL1 and YKL-40 were compared between the two groups,and multivariate Logistic regression models were used to analyze the influencing factors of cognitive im-pairment in AD patients.Based on the Mini-Mental State Examination(MMSE)score,the 110 AD patients were divided into mild cognitive impairment group(n=47),moderate cognitive impairment group(n=36),and severe cognitive impairment group(n=27).Spearman correlation analysis was performed to explore the relationships of serum CX3CL1 and YKL-40 with MMSE score,Addenbro-oke's Cognitive Examination-Ⅲ(ACE-Ⅲ)score,and Montreal Cognitive Assessment(MoCA)score.Results The AD group had higher proportions of patients aged over 80 years,with an edu-cation level of primary school or below,smoking history,alcohol consumption history,diabetes mel-litus,hypertension,AD family history,and living alone as well as higher serum levels of CX3CL1 and YKL-40 compared to the control group;conversely,the AD group had a lower proportion of pa-tients engaging in no physical exercise/labor,and lower MMSE,ACE-Ⅲ,and MoCA scores,with significant between-group differences(P＜0.05).Advanced age,diabetes mellitus,and high ser-um levels of CX3CL1 and YKL-40 were independent risk factors for cognitive impairment in AD pa-tients(P＜0.05),while an education level of college or above was a protective factor(P＜0.05).Compared with the mild cognitive impairment group,the moderate and severe cognitive impairment groups had higher serum levels of CX3 CL1 and YKL-40,and the severe cognitive impairment group had higher serum levels of CX3 CL1 and YKL-40 than the moderate group,with significant between-group differences(P＜0.05).Spearman correlation analysis revealed that serum CX3CL1 and YKL-40 were negatively correlated with MMSE,ACE-Ⅲ,and MoCA scores(P＜0.05).Conclu-sion Serum CX3CL1 and YKL-40 are highly expressed in elderly AD patients,and are closely re-lated to early cognitive impairment in elderly AD patients.

Objective To investigate the relationships of serum levels of human fractalkine(CX3CL1)and chitinase-3-like protein 1(YKL-40)with early cognitive impairment in elderly patients with Alzheimer's disease(AD).Methods A total of 110 AD patients in the Second Affiliated Hospital of Xinxiang Medical University from February 2021 to December 2023 were selected as AD group,and 50 healthy individuals with physical examination during the same period were selected as control group.Clinical materials and serum levels of CX3CL1 and YKL-40 were compared between the two groups,and multivariate Logistic regression models were used to analyze the influencing factors of cognitive im-pairment in AD patients.Based on the Mini-Mental State Examination(MMSE)score,the 110 AD patients were divided into mild cognitive impairment group(n=47),moderate cognitive impairment group(n=36),and severe cognitive impairment group(n=27).Spearman correlation analysis was performed to explore the relationships of serum CX3CL1 and YKL-40 with MMSE score,Addenbro-oke's Cognitive Examination-Ⅲ(ACE-Ⅲ)score,and Montreal Cognitive Assessment(MoCA)score.Results The AD group had higher proportions of patients aged over 80 years,with an edu-cation level of primary school or below,smoking history,alcohol consumption history,diabetes mel-litus,hypertension,AD family history,and living alone as well as higher serum levels of CX3CL1 and YKL-40 compared to the control group;conversely,the AD group had a lower proportion of pa-tients engaging in no physical exercise/labor,and lower MMSE,ACE-Ⅲ,and MoCA scores,with significant between-group differences(P＜0.05).Advanced age,diabetes mellitus,and high ser-um levels of CX3CL1 and YKL-40 were independent risk factors for cognitive impairment in AD pa-tients(P＜0.05),while an education level of college or above was a protective factor(P＜0.05).Compared with the mild cognitive impairment group,the moderate and severe cognitive impairment groups had higher serum levels of CX3 CL1 and YKL-40,and the severe cognitive impairment group had higher serum levels of CX3 CL1 and YKL-40 than the moderate group,with significant between-group differences(P＜0.05).Spearman correlation analysis revealed that serum CX3CL1 and YKL-40 were negatively correlated with MMSE,ACE-Ⅲ,and MoCA scores(P＜0.05).Conclu-sion Serum CX3CL1 and YKL-40 are highly expressed in elderly AD patients,and are closely re-lated to early cognitive impairment in elderly AD patients.

Objectives:Primary cardiac involvement (SSc-PHI) in systemic sclerosis is an important prognostic factor. We aimed to characterize and identify subclinical SSc-PHI using cardiovascular MRI to determine whether disease severity and serum biomarkers are associated with subclinical SSc-PHI.Methods:A total of 26 patients with SSc who had no history of cardiovascular disease or pulmonary hypertension underwent 3 T-enhanced cardiovascular MRI. Measurements included native T 1, extracellular volume, advanced gadolinium enhancement, T 2 mapping, and left ventricular volume function. Troponin T and N telencephalic natriuretic peptide precursors were also determined. Results:LGE was observed in 13 of 26 patients (50.0%), suggesting focal fibrosis, and T 2 mapping was significantly higher in the dcSSc group than in the lcSSc group ( P=0.009). Left ventricular volume and function were within the normal range in all patients, but final systolic left ventricular volume was significantly higher in dcSSc than in lcSSc ( P=0.021). The modified Rodnan skin score (mRSS) was significantly higher in patients with LGE focal fibrosis ( P=0.019). Logistic regression analysis confirmed the association between mRSS and LGE ( OR=1.224, P=0.037). In multivariate analysis, T 2 mapping was negatively correlated with disease course, and was correlated with dcSSc and fingertip ulcer ( R2=0.711, P=0.018, P=0.013, P=0.030). Troponin T was correlated with T 2 mapping ( r=0.555, P=0.049). Conclusions:Subclinical SSc-PHI is characterized by diffuse and focal myocardial fibrosis, but preserves myocardial systolic function. Subclinical SSC-Phi is associated with TNT, SSc disease severity, and complex peripheral vascular disease. These data provide information for identifying individuals at risk of SSc-PHI.