Objective To analysis the plague monitoring results on plague of rats in Lasiopodomys brandti plague natural foci of Xilin Gol Plateau in Inner Mongolia from 2001 to 2013,to master the dynamics of the plague epidemic,and to provide a basis for developing countermeasures.Methods Plague monitoring data from 2001 to 2013 in Lasiopodomys brandti plague natural foci were collected,main host density,rate of dye fleas,flea body index,bacteriology,serology and epidemic situation were studied.Results Within 13 years,10 153 Lasiopodomys brandti were captured overlapping a monitored area of 2 919.25 hm2,the average rat density was 3.48/hm2;other small rodents were captured 43 632 times,and 1 631 mice were captured,capture rate was 3.74％.Totally 22 752 host animals were checked by etiology,104 animals were infected with epidemic diseases,79 fleas of 31 groups positive fleas were checked from cultured 27 702 fleas of 6 437 groups.Totally 2 237 serum samples of Lasiopodomys brandti were checked using indirect hemagglutination (IHA),2 copies were found positive,the positive rate was 0.09％.Conclusion In Lasiopodomys brandti plague natural foci of Inner Mongolia,the plague bacteria infected host animals are still existed,future outbreak is possible;the monitoring and health education should be strengthened,in order to prevent the plague spreading to human being.

Objective To analyze the monitoring results on plague in Meriones unguiculatus plague natural foci in Inner Mongolia from 2010 to 2014,to master the epidemic dynamics of the plague and to provide a basis for developing countermeasures.Methods The plague monitoring data in Meriones unguiculatus plague natural foci from 2010 to 2014 were collected;main host density,rate of dye fleas,flea body index and bacteriology were counted;serology detection was done and the epidemic situation was analyzed.Isolation and identification of Yersinia pestis were carried out through a 4-step test (microscopic examination,isolation and culture,phage lysis test and animal experiment).Serum samples were tested by indirect hemagglutination test.Results Within 5 years,21689 Meriones unguiculatus were captured overlap monitored areas of 7116 hm2 totally,the average rat density was 3.05/hm2;other small rodents were clipped 144352 times,3947 mice were captured,capture rate was 2.73％.Totally 26500 Meriones unguiculatus were checked,91 Meriones unguiculatus were infected with epidemic diseases,227 of 59 groups positive fleas were checked from cultured 51262 fleas of 13268 groups.Totally 5426 serum samples of Meriones unguiculatus were checked,5 copies were found positive,the positive rate was 0.09％.Conclusions Inner Mongolia Meriones unguiculatus plague is still active and spreading.We must enhance propaganda of the plague.Surveillance and emergency management should be strengthened to prevent a outbreak of the plague in human being.

This is a complicated and difficult case. The onset symptom of a 62-year-old male was recurrent intestinal obstruction. Ileocecal and ileocolic operation was done twice. Massive gastrointestinal bleeding occurred due to giant fistula of descending duodenum, which connected to ileocolic anastomosis. After consultation by multidisciplinary team, jejunal-feeding tube was placed to provide enteral nutrition. With general condition improving, duodenal fistula repair and involved bowel resection were performed. Postoperative pathology confirmed Crohn's disease. The patient was treated with thalidomide and recovered well during follow-up.

Objective:To investigate the differential expression of cyclooxygenase (COX) gene between familial aggregated atherosclerotic large-artery atherosclerosis(LAA) cerebral infarction patients and sporadic LAA cerebral infarction patients.Methods:Twenty-five patients with familial aggregation LAA cerebral infarction (familial aggregation LAA group) and 25 sporadic LAA cerebral infarction patients (sporadic LAA group) were collected.Another 25 patients with non-cardiovascular and cerebrovascular diseases were selected as control group.Real-time quantitative PCR and ELISA were used to detect COX-1 and COX-2 protein in the supernatant of samples.Results:The expression level of COX-1 gene was 0.5436±0.2737, 0.2400±0.1656 and 0.8340±0.3799 in the familial aggregation, sporadic LAA cerebral infarction and control groups.Compared with control group, COX-1 gene expression in sporadic LAA cerebral infarction group and familial aggregation LAA cerebral infarction group was significantly down-regulated, the differences were significant( P<0.05). The expression level of COX-2 gene was 1.3672±0.8249, 1.3932±0.7158 and 0.7212±0.9623 in the familial aggregation, sporadic LAA cerebral infarction and control groups.Compared with the normal control group, the expression of COX-2 gene in familial aggregation LAA cerebral infarction group and sporadic LAA cerebral infarction group increased significantly( P<0.05). The expression of COX-1 protein was 2.9956±0.5672, 2.5572±1.0289 and 2.6721±0.7944 in the familial aggregation, sporadic cerebral infarction and control groups.The expression of COX-2 protein was 16.63±4.06, 16.86±7.93 and 15.94±5.94 in the familial aggregation, sporadic cerebral infarction and control groups.There was no significant difference in the relative expression of COX-1 and COX-2 proteins among familial aggregation LAA cerebral infarction group, sporadic LAA cerebral infarction group and control group(all P>0.05). Conclusion:There is significant difference in COX-1 gene expression between patients with familial LAA and sporadic LAA cerebral infarction.

OBJECTIVETo explore apoptosis of multiple myeloma (MM) cells and its mechanism by the combined inhibition of mTORC2 signaling pathway and heat shock protein 90.

METHODSThe effects of Rapamycin, 17-AAG and the combination on proliferation of MM cell lines U266 and KM3 were assessed using MTT at different time points (0, 8, 24, 48 hour). Cell apoptosis and cell cycle distribution were measured by flow cytometry. The specific proteins p-AKT (ser473), p-AKT (thr450), p-S6 (S235/236) and AKT were detected by Western blotting.

RESULTSRapamycin, 17- AAG and the combination suppressed the proliferation of MM cell lines U266 and KM3, especially the combination of Rapamycin and 17-AAG synergistically inhibited the proliferation (P<0.05); Rapamycin induced G1 arrest both at 24 and 48 hours, 17-AAG also induced G1 arrest, especially at 48 hours (P<0.01); Rapamycin, 17-AAG alone decreased the expression of AKT and induced MM cell apoptosis to some extent (P<0.01); Chronic rapamycin treatment inhibited mTORC2; Inhibition of both mTORC2 and chaper on pathways degraded AKT and induced MM cell apoptosis, which was significantly higher than that of any single agent (P<0.01).

CONCLUSIONInhibition of both mTORC2 and chaper on pathways decreased the expression of AKT to induce apoptosis of MM cells in vitro.

Apoptosis ; Benzoquinones ; pharmacology ; Cell Cycle ; Cell Division ; Cell Line, Tumor ; drug effects ; HSP90 Heat-Shock Proteins ; metabolism ; Humans ; Lactams, Macrocyclic ; pharmacology ; Mechanistic Target of Rapamycin Complex 2 ; Multiple Myeloma ; pathology ; Multiprotein Complexes ; antagonists & inhibitors ; metabolism ; Proto-Oncogene Proteins c-akt ; metabolism ; Signal Transduction ; Sirolimus ; pharmacology ; TOR Serine-Threonine Kinases ; antagonists & inhibitors ; metabolism