Objective To explore the effects of music nursing on relieving vasospasm and pain in premature infant during peripherally inserted central catheter (PICC), so as to provide reference for the popularization and utilization of music nursing. Methods A total of 35 premature infants with vasospasm during the course of PICC catheterization from June 2015 to January 2017 were selected as the experimental group, and given music nursing. Another 35 premature infants with vasospasm during the course of PICC catheterization between January 2014 and June 2015 were selected as the control group, and given routine nursing care. The pain degree was compared by Premature Infant Pain Profile (PIPP), and the relieving degree of vasospasm was monitored at the same time. Results After the intervention, the score of PIPP of the two groups both decreased. The PIPP score of the experimental group was (7.12±2.03), while that of the control group was (8.14±2.12), and the difference was statistically significant (t=2.600,P<0.05). In the experimental group, there were 11 cases of complete remission and 24 cases of mild remission. In the control group, there were 8 cases of complete remission, 20 cases of mild remission and 7 cases of no remission. The difference between the two groups was statistically significant (χ2=7.837,P<0.05). After the intervention, the sleep time of the children increased in both groups (P<0.05). The sleep time of the experimental group was higher than that of the control group, and the difference was statistically significant (P<0.05). Conclusions Music nursing can effectively relieve vasospasm and pain in children during PICC catheterization which is worthy of popularization and application.