Objective To explore the effects of anatomical and nonanatomical hepatectomy on the prognosis of patients with hepatocellular carcinoma(HCC).Methods A total of 62 HCC patients were enrolled,including 33 cases of anatomical hepatectomy(Group AH)and 29 cases of nonanatomical hepa-tectomy(Group IH).The operation situation and hematocrystallin(HB)loss were record.Fasting venous blood of HCC patients were extracted for detection of circulating AFP mRNA expression.The recurrence, mortality and complication rate were recorded at the same time.Results The operative time of Group AH was significantly higher than that of Group IH,while the bleeding volume and HB loss were lower(P<0.05).There were no significant differences in AFP mRNA expression and recurrence rate.The short-term mortality rate of Group AH was lower than Group IH(P<0.05 ),but their long-term mortality rates were similar(P>0.05 ).There was no significant difference in complication rate between groups.Patients of Group AH got more hepatosis due to liver cirrhosis or fibrosis,while patients of Group IH got more bleeding or infection due to the central tumor.Conclusion Anatomical hepatectomy has advantages in protecting artery and improving prognosis for HCC patients,but it has surgical risks for patients with serious patholog-ical damage in hepatic lobes,and nonanatomical hepatectomy may be considered.

Objective To summarize the nursing care and cooperation with surgeons using transoral Orvil EEA stapler during total laparoscopic gastrectomy (TLG).Methods We present our initial experience of nursing care and cooperation with surgeons for 8 patients undergoing TLG using a circular end-toside anastomosis created with the transoral directed EEA circular stapler Orvil.Results The surgeries of all 8 patients were successfully completed under our good cooperation with surgeons using the technique of Orvil system.Conclusions Good preparations and knowing well the surgical procedures were the key points to achieve success using the transoral Orvil EEA stapler for patients undergoing TLG.

Hepatic satellite cell (HSC) is a kind of nonparenchymal cells in the liver,which releases various collagenous fibers and cytoskeletal proteins,playing an important role in the pathophysiological changes of the liver.In normal,HSCs maintain normal tissue architecture via regulating the synthesis and degradation of extracellular matrix (ECM) proteins.During liver injury,HSCs were activated.Activated HSCs are the main cells that lead to the accumulation of extracellular matrix,which is the main reason of hepatic fibrosis,cirrhosis and liver failure.Therefore,lucubrating the role and mechanism of HSC in the progress of hepatic disease and investiating the HSC-related therapeutic strategies have practical significances for the prevention and treatment of liver injury and raise of patients' survival rates in clinical practice.

MicroRNA ( miRNA) is a family of 19 - 24 nucleotides small non - coding RNA in eukaryotic organisms , which can regulate genes at the post - transcriptional level. miRNA has been shown to be involved in a variety of cellular processes such as development, proliferation, differentiation, apoptosis etc, and is closely related to the oncogene-sis. Recent study indicates that several miRNAs play an important role in initiation and progression of hematological malignancies. miRNA expression profiling has identified signatures associated with diagnoses, prognoses and therapy of hematological malignancies. The review discusses miRNA and its biogenesis, functional mechanisms and potential role in the diagnosis , prognoses and therapy of hematological malignancies.

With the development of molecular mechanisms of hepatocelluar carcinoma,targeted therapy has been a research hotspot in recent years.Some target agents of hepatocellular carcinoma have been approved.We summarize the progress of targeted therapy for hepatocellular carcinoma in this paper.

With the improvement of endoscopic retrograde eholangiopancreatography (ERCP) technology, endoscopy is not only applied to diagnosis, but also utilized to therapy for cholangiopancreatic neoplasm. This article is to summarize the application of endoscopic technology on cholangiopancreatic neoplasm.

Objective Observe the pupil constrict response to the pilocarpin,identify the difference of the response between the young and the old.Methods Use the infrared digital camera of sonyF717 to record the dynamic variety of the pupil constrict response to the 1%pilocarpine liposome and the pilocarpine liquid in dark environment.Then deliver the data to the computer,measure the data by imagetool software,analysis the data by the Sigamastat software.Results In darkness,the pupil diameter of the young is equally 6.8?0.643mm,the old is equally 4.4?0.447mm.T test t=16.1,P

Ontogenesis of gastrin cells (G cells) and somatostatin cells (D cells) in the gastrointestinal tract was studied by PAP and indirect immunofluorescence method in sixty human fetuses aged 8-38 weeks of gestation. The appearance of D cells and G cells was observed as early as 8-9 weeks of gestation. These cells were not seen in the submucosa and the tunica muscularis except the epithelium of gut. After 12 weeks of gestation, D cells can be found in the mucosa of the whole gut, whereas G cells only in the mucosa of the antrum and small intestine. The distribution, number and the ratio of G cells to D cells were also examined. In addition, we have observed that G cells in the antral mucosa often possessed processes which were considered to have paracrine function. The G cells and D cells were open-type endocrine cells in the human fetal gut except the D cells in the fundic mucosa. The possible function of G cells and D cells in the development of gut was discussed. The ratio of the two cell types in adult human antrum and upper duodenum has also been compared with that of fetus.

Objective To evaluate the diagnostic value of the combined detection of serum Fer,AFP and AFP-L3 in patients with primary hepatic cancer (PHC).Methods The serum levels of Fer,AFP and AFP-L3 were determined in 92 patients with PHC,84 patients with benign liver disease,and 45 control subjects.Results The PHC group serum Fer was (819.4±616.8) μg/L,AFP was (556.9±451.5) μg/L,AFP-L3 was (109.0±62.5) μg/L.The benign liver disease group Fer was (229.3±155.5) μg/L,AFP was (6.0±4.2) μg/L,AFP-L3 was (26.0±49.4) μg/L.The healthy control group Fer was (125.6±110.5) μg/L,AFP was (2.8±0.8) μg/L,AFP-L was (7.0±1.8) μg/L,there was significant difference between the three groups (all P ＜ 0.01).The sensitivity of PHC group alone in detection of serum Fer was 85.9 ％,the sensitivity of AFP was 71.0 ％,the sensitivity of AFP-L3 was 83.7 ％,the combined detection of Fer and AFP or AFP-L3 improved the sensitivity of the test to 90.2 ％ and 95.7 ％,the three combined detection sensitivity was 98.9 ％.Conclusion Combined detection of serum Fer,AFP and AFP-L3 is essential in the diagnosis of PHC.

Objective To observe the correlation between postmenopausal estrogen levels and diabetic retinopathy (DR) in women.Methods Thirty-nine menopause female patients with type 2 diabetes mellitus and 17 menopause subjects (control group) were enrolled in this study.Control subjects aged from 53 to 82 years,with the mean age of (69.80± 8.32) years.Diabetes mellitus patients aged from 56 to 84 years,with the mean age of (70.50±8.27) years;diabetes duration ranged from 3 to 23 years,with the average course of diabetes (11.40± 7.97) years.DR diagnosis was according to the results of fundus fluorescein angiography,and thus the 39 patients were divided into DR group (19 patients) and non-DR (NDR) group (20 patients).There was no significant difference in age and menopause duration between the three groups (t=0.347,0.485;P＞0.05).There was significant difference in diabetes course (t=2.748,P＜0.05).Compared with NDR group,fasting blood glucose (FBG),glycosylated hemoglobin (HbAlc),total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL-C) were significantly increased (t=6.130,5.322,4.574,2.426,4.033),high density lipoprotein cholesterol (HDL-C) was significantly lower (t=3.917),the difference was statistically significant (P＜0.05).The level of estradiol (E2) was measured by radioimmunoassay.The differences of E2 levels between the three groups were compared.Logistic regression analysis was used to analyze the influencing factors of DR.Results The levels of E2 in control group,DR group and NDR group were (42.38 ±8.64),(21.49 ± 9.81) and (32.72 ± 10.51) pg/ml,respectively.The level of E2 in DR group was significantly lower than that in NDR group and control group (t=3.443,10.110;P＜0.05).Logistic regression analysis showed that the duration of diabetes mellitus [coefficients =0.166,odds ratio (OR)=1.181,P=0.016],FBG (coefficients=1.162,OR=4.014,P=0.001),TC (coefficients=3.212,OR=10.820,P=0.002),TG (coefficients=1.649,OR=5.203,P=0.030) and LDL-C(coefficients=1.605,OR=4.976,P=0.003) were the risk factors for DR;E2 (coefficients=-0.100,OR=0.904,P=0.004) and HDL-C (coefficients=-4.460,OR=0.012,P=0.002) were the protective factors for DR.Conclusion The estrogen level of postmenopausal women have a certain correlation with the development of DR,it may be one of the protective factor of DR.