Papillary thyroid cancer (PTC) is the most common type in thyroid carcinomas.Recently,the prevalence and diagnostic rate of PTC has got significantly high with the general use of ultrasound.Nowadays,more and more studies have suggested that the coexistence of PTC and diabetes is common.They indicated that hyperglycemia would induce the deterioration of oxidative stress injury,chronic inflammation,insulin resistance,obesity,dyslipidemia,deficiency of vitamin D and dysimmunity.All of these may break the balance of oxidation and antioxidation and result in disordering signal pathway,accumulation of inflammatory cytokines,over activating of insulin and insulin-like growth factor-1,changing the metabolic pathways,which will promote the occurrence and progression of PTC.

Objective:To study the relationship of stressful life events,coping styles and emotional disorder.Methods:345 junior middle school students were collected as sample.They were tested with Stressful Life Event Questionnaire,Coping Style Questionnaire,SAS and SDS.Results:The coping style played an important role on the anxiety and depression;and stressful life events influenced the anxiety and depression indirectly through the coping styles.Conclusion:Anxiety and depression were determined by the emotional-focused coping style.

Objective To explore the impacts of 75％ low-protein diet intake during gestation on fetal growth restriction (FGR) rat model establishment.Methods Thirty-eight pregnant Sprague-Dawley rats were included into the study.At first,five pregnant rats were fed with sufficient normal diet with protein content of 22％.Their daily food consumption was recorded and taken as the basis to determine daily feed consumption of 75％ low-protein group (protein content 9.2％).In order to ensure that each group finally had at least ten pregnant rats to deliver,there were 11 rats assigned to the control group (pregnant rats fed with sufficient normal diet,protein content was 22％),13 to the low-protein group (pregnant rats fed with low protein diet,protein content was 9.2％,but the food consumption was the same as control group) and 14 to the 75％ lowprotein group (pregnant rats fed with low-protein diet,protein content was 9.2％,the food consumption was 75％ of the control group).All female rats were fed with sufficient normal diet after delivery.The body weight,overall weight gain during gestation,the mortality rate and the non-delivery rate of pregnant rats were compared.The third day's newborn weight after birth,FGR incidence and the mortality rate within three days after birth of newborns were also compared.One way analysis of variance,LSD-t test,independent sample t-test and Chisquare test were used as statistical methods.Results (1) The body weight of pregnant rats:There was no significant difference in body weight among the three groups at gestational day 0,3 and 6.On day 9,body weight of 75％ low-protein group [(271.9±8.4) g] and low-protein group [(274.1 ±7.8) g] were significantly lower than that of the control group [(287.2± 18.7) g] (t=2.514 and 2.170,both P＜0.05),but there was no significant difference between the former two groups.On Day 12,body weight of 75％ low-protein group [(275.7 ± 10.7) g] and low protein group [(285.1 ± 12.5) g] were significantly lower than that of the control group [(306.4±29.7) g] (t=3.262 and 2.218,both P＜0.05),and the difference between the former two groups was also statistically significant (t=2.098,P＜0.05).Before delivery,body weight of 75％ low-protein group,low protein group and control group were (300.4±14.1) g,(317.0±16.3) g and (372.9±19.1) g,respectively with statisticall significance (F=64.219,P＜0.05).The overall weight gain during pregnancy for 75％low-protein group,low-protein group and control group was (61.6± 19.8) g,(81.8±21.6) g and (139.3± 12.0) g,respectively.The difference among the three groups was statistically significant (F=55.863,P＜0.05).(2) The mortality rates of pregnant rats for 75％ low-protein group,low-protein group and control group were 3/14,2/13 and 1/11 respectively without significant difference (P＞0.05).Neither was the non-delivery rate within 30 days (embryonic resorption) for the three groups (1/14,1/13,0/11,P＞0.05).(3) The numbers of pups were 101 in 75％ low-protein group,104 in low-protein group and 107 in control group.The newborn mortality rate within three days after birth was 28.7％ (29/101) in 75％ tow-protein group and 23.0％ (24/104)in low-protein group,with were significantly higher than that of the control group (7.5％,8/107) (x2=16.022and 9.976,both P＜0.05),but there was no significant difference between groups.The third day's newborn weight after birth for 75％ low-protein group,low-protein group and control group were (6.3 ±0.8) g,(6.9±0.9) g and (8.1 ±0.9) g,the difference was statistically significant (F=90.602,P＜0.05).FGR incidence for 75％ low-protein group was 55.6％ (40/72),which was significantly higher than that of the low-protein group (28.8％,23/80) and the control group (5.0％,5/99) (x2=11.220,54.834 and 18.833 all P＜0.05).Conclusion 75％ low-protein diet feeding during pregnancy is an ideal method to induce FGR rat model with high FGR incidence,whereas and low mortality rates of pregnant rats,the fetuses and newborns.

Objective To explore the value of neutrophils gelatinases related lipid a carrier protein (NGAL),renal injury molecule-1 (KIM-1) and serum creatinine (SCr) for early diagnosis in adult cardiac surgery after acute renal injury (AKI).Methods Blood and urine samples of 65 cardiac surgery patients before and 24,48,72 h after surgery were collected,SCr,urine NGAL and KIM-1 levels was detected,among whom AKI occurred in 34 cases(AKI group),did not occur in 31 cases (non AKI group),and they were compared with 20 cases of healthy physical examination (control group).Logistic regression model was used to judge the value of early diagnosis of SCr,urine NGAL and KIM-1.Results The incidence of AKI after cardiac surgery was 52.31％ (34/65).The SCr,urine NGAL and KIM-1 levels in AKI group and non AKI group before and 24,48,72 h after surgery were significantly higher than those in control group [SCr:(62.44 ±22.26),(77.38 ±24.38),(98.24 ±25.02),(98.14 ±45.26)μmol/L and (56.67 ±21.68),(59.25 ± 15.58),(55.40 ±22.91),(40.10 ±23.45)μmol/L vs.(16.43 ±5.01)μmol/L;urine NGAL:(4.81 ±0.65),(9.48 ±3.29),(11.87 ±3.93),(7.66 ± 1.52) μg/L and (4.67 ±0.53),(8.89 ± 2.64),(11.46 ± 3.60),(7.47 ± 1.63)μg/L vs.(2.87 ± 0.48) μg/L; KIM-1:(47.93 ± 5.54),(61.89 ± 12.44),(100.21 ± 27.32),(67.69 ± 8.72) μ g/L and (47.12 ± 4.08),(56.38 ± 9.27),(90.14 ± 27.26),(69.40 ±8.90) μ g/L vs.(32.21 ± 12.54)μ g/L] (P ＜ 0.01).Urine NGAL and KIM-1 levels in AKI group and non AKI group 24,48,72 h after surgery were significantly higher than those before surgery (P＜ 0.01).SCr,urine NGAL and KIM-1 levels in AKI group 24 h after surgery were significantly higher than those in non AKI group (P＜ 0.01).Twenty-four hours after surgery,the sensitivity and specificity of urine NGAL,KIM-1 in the diagnosis of AKI,compared with that of SCr had no statistical significance (P ＞ 0.05).Conclusions The adults who accept extracorporeal circulation cardiac surgery appear high incidence of AKI.Twenty-four hours after surgery,early biological markers-urine AKI,KIM-1,has similar sensitivity and specificity with traditional diagnostic gold standard of SCr.

Objective To investigate the clinical significance of the glucose screening tests during pregnancy. Methods 1 516 cases of 24～28 weeks pregnant women were given 50g glucose screening tests, and for those whose blood glucose ≥7. 8 mmol/L further underwent glucose tolerance tests(OGTT) for a clear diagnosis. Results The incidence of gestational diabetes is 3.17% and the incidence of impaired glucose tolerance in pregnancy is 2. 64%. Conclusion 50g glucose screening for pregnant women helps to carry out early diagnosis and treatment of gestational diabetes,to improve the quality of obstetric and reduce perinatal mortality rate,it is of great significance.

This study was designed to validate the correlation between integrated pharmacokinetic and therapeutic effects of alkaloids using bile processed Rhizoma Coptidis (BRC). Rats were divided into three groups: normal, disease model, model+BRC. Rats were induced to have an excessive heat syndrome. Rectal temperatures were collected at 0, 3, 6 and 9 h after single oral administration of the drugs. The plasma concentrations of three alkaloids were quantified at different times by UPLC-MS/MS after the administration of BRC. An approach of self-defined weighting coefficiency was created to the holistic pharmacokinetic profiles of alkaloids in BRC. The classified and integrated synthetic concentrations were obtained, and then the pharmacokinetic parameters of alkaloids were calculated from non-compartmental model analysis. The potential relationship between the integrated mean concentration of alkaloids and the antifebrile efficacy was investigated. The holistic t(max) of alkaloids was 1.11 h, the antifebrile effect of BRC at 3 h was improved over the model group. Double peaking appeared in the integrated blood concentration-time curve, the second t(max) of alkaloids was 4.82 h. The antifebrile effects of BRC at 3-6 h were significant, and the antifebrile effects at 6-9 h was decreased significantly. Dynamic variation of alkaloids of BRC in the body exhibited the similarity to the pattern of its antifebrile effect.
Administration, Oral ; Alkaloids ; pharmacokinetics ; Animals ; Bile ; Coptis ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacokinetics ; Rats ; Rats, Sprague-Dawley ; Rhizome ; chemistry ; Tandem Mass Spectrometry

OBJECTIVEThis study aimed to investigate the duration of gingival healing after the stage II surgery of dental implantation for periodontitis patients and to provide clinical guidelines for implant restoration.

METHODSTwenty-nine periodontitis patients who had implantation surgery and achieved osseointegration were operated with stage II surgery (a total of 60 pieces of implants). The height of buccal gingival of each implant was measured twice after the stage II surgery. All implants were measured at the lowest point ofbuccal gingival after one week. The implants were randomly divided into four groups according to the schedule of the next test time: group one at one week from the initial test point, group two at two weeks, group three at three weeks, and group four at four weeks. Each group includes 15 pieces of implants. The amount of the buccal gingival change in each group between the second and first tests was determined, and the data were analyzed statistically.

RESULTSThe amount of gingival change of groups one, two, three, and four was (-0.25 +/- 0.66), (-0.04 +/- 0.52), (-0.70 +/- 0.77), and (-0.74 +/- 1.09) mm, respectively. No significant difference was observed between groups one and two in terms of the amount of gingival changes (P > 0.05). However, a significant difference was found between groups two and three (P < 0.05), and the amount of gingival recession was 0.66 mm. No significant difference was found between groups three and four (P > 0.05), and the gingival achieved stability.

CONCLUSIONThe gingival recession achieves stability at the fourth week (after 28 d) after stage II surgery. At this time, the implant can be restored, and the abutment can be selected according to the amount of gingival change of the periodontitis patient.

Alveolar Bone Loss ; Dental Implantation ; Dental Implantation, Endosseous ; Dental Implants ; Dental Restoration Failure ; Gingiva ; Gingival Recession ; Humans ; Osseointegration ; Periodontitis

Adult ; Cord Blood Stem Cell Transplantation ; adverse effects ; Cytomegalovirus ; Cytomegalovirus Infections ; complications ; Female ; Hemolysis ; Humans

Background Keratectasia after laser in situ keratomileusis (LASIK) is one of the serious complications which affect the vision.The measurement of cornea posterior elevation is an important way helping to find this change.Objective The aim of this study was to investigate the change of cornea posterior elevation after LASIK in myopic eyes with astigmatism and affecting factors.Methods A series case-observational study was adopted.One hundred and twenty-seven myopic eyes with astigmatism of 66 patients who received LASIK in 2008 May through 2010 January in Beijing Tongren Hospital were retrospectively analyzed to evaluate the change of cornea posterior elevation following the LASIK.The parameters related to cornea posterior elevation were measured and compared before and 3 months,6 months and 1 year after surgery,respectively,with Oculyzer anterial segment analysis system.Results The height values from posterior cornea highest point,the lowest point and central vertex central elevation zone were (12.20±3.39),(-19.02±7.38) and (1.05 ±3.25) μm respectively before LASIK and were (14.38±3.80),(-18.55±7.11),(2.83±4.81)μm in3 months and (13.99±3.38),(-17.57±6.54),(2.45±4.61) μm in 6 months after LASIK.They were (14.40±3.85),(-17.76±6.00),(2.16±5.00) μm in 1 year after surgery.Significant increases were found in the vertex height and central elevation after LASIK compared with before surgery(highest pointq=6.813,5.594,6.875,all at P＜0.001.central vertex:q=4.488,P=0.002;q =3.530,P=0.013 ;q =2.799,P =0.047).However,no significant difference was seen in various time points after LASIK (P＞0.05).A positive correlation was obtained between the height value of the posterior cornea central vertex with the spherical equivalent (SE),maximum cutting depth or cutting proportion 1 year after LASIK (r =0.295,0.297,0.295,all at P=0.001),and there was a negative correlation between it with residual stroma (r=-0.208,P=0.019).The intraocular pressure (IOP) measured with noncontact tonometry (NCT) was (14.24±3.33) mmHg before LASIK,and those of 3 months,6 months and 1 year were (8.42± 1.90),(8.61 ± 1.64) and (8.76± 1.64)mmHg after LASIK,showing a significant lowing in IOP after LASIK in comparison with before LASIK (q =29.851,28.317,26.337,all at P＜0.001).But no significant change was found in the different time points after LASIK (P＞0.05).There was significant difference in the IOP corrected by Ehlers after LASIK (P＞0.05).The IOP before LASIK had positive correlation with the central elevation of posterior cornea surface 1 year after LASIK(r=0.258,P =0.003).Conclusions The cornea posterior elevation increases slightly early stage after LASIK but retains stable level with lapse of time.The refraction diopter before LASIK is the main factor affecting the posterior corneal shape.To reduce the risk of corneal ectasia,more attention should be paid to the reserving of appropriate corneal residual stroma and smallest degree of eccentricity during the ablation procedure.

This study was designed to validate the correlation between integrated pharmacokinetic and therapeutic effects of alkaloids using bile processed Rhizoma Coptidis (BRC). Rats were divided into three groups: normal, disease model, model+BRC. Rats were induced to have an excessive heat syndrome. Rectal temperatures were collected at 0, 3, 6 and 9 h after single oral administration of the drugs. The plasma concentrations of three alkaloids were quantified at different times by UPLC-MS/MS after the administration of BRC. An approach of self-defined weighting coefficiency was created to the holistic pharmacokinetic profiles of alkaloids in BRC. The classified and integrated synthetic concentrations were obtained, and then the pharmacokinetic parameters of alkaloids were calculated from non-compartmental model analysis. The potential relationship between the integrated mean concentration of alkaloids and the antifebrile efficacy was investigated. The holistic t(max) of alkaloids was 1.11 h, the antifebrile effect of BRC at 3 h was improved over the model group. Double peaking appeared in the integrated blood concentration-time curve, the second t(max) of alkaloids was 4.82 h. The antifebrile effects of BRC at 3-6 h were significant, and the antifebrile effects at 6-9 h was decreased significantly. Dynamic variation of alkaloids of BRC in the body exhibited the similarity to the pattern of its antifebrile effect.