Herpes simplex keratitis (HSK),which is caused by infection of herpes simplex virus,is a kind of disease with high incidence and blinding rate.The mechanism of HSK is complex and always attracts the attention of researchers.It is considered as an immune response to viral infection and is related to several factors,such as pathway of infection and immunoregulation.This review reveals the latest advance of the mechanism of HSK in the aspects of viral infection and latency,apoptosis,immunity.

AIM: To establish the determination of baicalin and hesperidin in Muxiangliqi Pills (Radix Saussureae seu Vladimiriae, Pericarpium Citri Reticulatae Viride, Pericarpium Citri Reticulatae, Rhizoma Cyperi, etc.). METHODS: The determination was carried out by HPLC with octodelyl silicohydrides silanes bonded silica gel as a packing material, methanol water glacial acetic acid (35∶61∶4) as a mobile phase, detection wavelength at 283nm, flow rate at 1mL?min -1 . RESULTS: Hesperidin showed a good linear relationship at the range of 0.072?g ～0.360?g. Baicalin showed a good linear relationship at the range of 0.104?g～0.520?g. The average recoveries were 97.0% and 97.8%, respectively. RSD were 1.93% and 1.69%, respectively. CONCLUSION: The method is sensitive, accurate, reproducible, specific and can be used for quality control of Muxiangliqi Pills.

Objective To explore the impact of intrapartum mild asphyxia on motor development in infants. Methods 30 infants with any of the following were as asphyxia group: heart rate more than 160 beats/min in the process of childbirth, and then down to 70-80 beats/ min, no cyanose; Apgar score 1 min more than 8. Other 30 normal infants were as control group. They were assessed with Neuropsychological Development Scale for 0-6 Years Old 3, 6 and 9 months after birth. Results Opisthotonos was more frequent in the asphyxia group than in the control group (P<0.01) 3 months after birth, but there was no significant difference in total score (P>0.05). Sitting forward was more frequent in the asphyxia group 6 months after birth, and the score of motor was less in the asphyxia group than in the control group (P< 0.05). More of the asphyxia group couldn't stand with help, and with poor motor score 9 months after birth (P<0.05). Conclusion Intrapartum mild asphyxia may infulence the motor development of infant in long-term.

Objective To explore the effect of laparoscopy and laparotomy surgery on immune function related indicators in ovarian benign tumor patients. Methods From January 2015 to January 2016, 86 cases of ovarian benign tumor patients were set as study objects. All the patients were randomly divided into control group and observation group, 43 cases in each. Patients in control group were given traditional laparotomy surgery, while patients in observation group were given laparoscopic surgery. The changes of immune function related indexes before and after operation were recorded, before operation, day 1 and day 3 postoperative, number of peripheral white blood cells (WBC), neutrophil ratio (NGR), immunoglobulin (IgA, IgG, IgM) levels, C reactive protein (CRP) content and the distribution of T lymphocyte subsets (CD3+, CD4+ and CD3+/CD4+ ratio) distribution were respectively determined, and the incidence of postoperative complications was recorded, and were further statistical analyzed. Results There was no significant difference in immunoglobulin IgA, IgG and IgM between the twogroups before operation (P > 0.05). Postoperative, only the IgG content of the two groups was significantly lower than that before the operation (P < 0.05 ); The levels of WBC, NGR and CRP in the control group patients in the昀rst day and the third day after surgery were significantly increased compared with preoperative period, while CRP in the observation group was higher than that before the operation, there was significant difference between the two groups (P < 0.05). There was no significant difference in CD3+, CD4+ and CD3+/CD4+ ratios between the two groups before operation (P > 0.05), the difference between the two groups was significant (P < 0.05). The complication rate of the observation group after operation was 6.98%, the control group was 16.28%. Conclusion Clinical use of laparoscopic treatment of benign ovarian tumors relative to the traditional laparotomy can effectively reduce the immune system of patients with immune function, with high safety, contribute to the recovery of patients, it is worthy of clinical promoting.

Objective To determine the role of CTGF in PVR, we successfully down - regulated the expression of CTGF by RNA interference( RNAi) technology in ARPE-19 cell line. Methods Design and synthesis three CTGF siRNA, screen effective targeted points in Western blot, combine Oligo DNA of effective targeted point, anneal to form double chain DNA, connect with linearing pGC-LV-GFP carrier, sequence DNA in PCR screening positive clones. Results The shRNA-expression vector of CTGF was constructed successfully. And RT-PCR and Western Blot results showed that CTGF expression was significantly inhibited by siRNA transfectants in ARPE-19 cells at mRNA and protein levels. Conclusion We successfully construct shRNA-expression vector of CTGF which paves a way for CTGF-targeted gene therapy of proliferative vitreoretinopathy.

Objective:Discussing the audit idea of equipment and material procurement in health system under the latest requirements of the administrative institutions internal control specification and Chinese internal auditing standards.Methods: According to the specific requirements of the new specification and the new standard, combined with the practical work experience and the review of the literature, to discuss the audit idea of equipment and material procurement in health system, based on the perspective of internal control audit of procurement and payment cycle.Results: Systematic reviewing and standardized summarizing the procedure, content and method of the equipment and material procurement audit in health system were used.Conclusion: It has been found that audit of fine design under the new specification and the new standard could well improve the work quality and work effectiveness of the internal audit in health system.

Objective To investigate the effect of Pill of Cyperus and Amomum with Six Noble Ingredients in the treatment of gastrointestinal symptoms after chemotherapy in gynecologic cancer patients in serum IgM, IgA and IgG.Methods 72 cases with gastrointestinal symptoms after chemotherapy in gynecologic cancer were selected and all patients were under the IP scheme,and patients were randomly divided into 2 groups,36 cases each.Control group received conventional therapy,study group received more with Pill of Cyperus and Amomum with Six Noble Ingredients,immune functions and peripheral blood, symptom score and KPS score and clinical effect were compared after the treatment.Results Compared with before treatment,2 groups of WBC,NEU,HGB level decreased(P<0.01),CD3 +,NK level,KPS score increased(P<0.05).Compared with the control group, levels of WBC,NEU,HGB in the study group were higher(P<0.01),CD3 +,CD4 +,NK,serum IgG,IgM,IgA levels were higher(P<0.05).Nausea, vomiting,acid regurgitation,appetite symptoms score were lower(P<0.05),KPS scores was higher(P<0.05),the effective rate of the the study group was higher ( P <0.01 ) .Conclusion Pill of Cyperus and Amomum with Six Noble Ingredients in the treatment of gastrointestinal symptoms after chemotherapy in gynecologic cancer patients was curative effect,and it can improve immune function and quality of life and elevated levels of serum IgG, IgM and IgA.

Objective]To investigate the application value of four-dimensional ultrasound with high definition flow(HD flow) in the observation of fetal pulmonary vein.[Methods]From June 2015 to June 2016,130 fetuses in our hospital underwent prenatal fetal echocardiography were included in this study. Color Doppler and high definition flow imaging were used to observe and count the number of pulmonary veins in the fetal four chamber view. In the condition of opening HD flow ,the 3D volume data was collected from the heart of the fetal four chamber view,and the 3D images of the fetal pulmonary vein were obtained by using the post processing software. Comparisons were made between the three methods for visualization numbers of the normal fetal pulmonary veins. For 5 cases of abnormal pulmonary venous drainage,STIC combined with HD flow technique was used to carry out three-dimensional reconstruction of pulmonary veins,to observe the abnormal drainage.[Results]The display rate of HD flow on fetal pulmonary veins was higher than that of color Doppler. For the 17~32 weeks fetal,four-dimensional ultrasound combined with HD flow could better observe the spatial location of pulmonary veins.[Conclusions]STIC combined with HD flow technology can display fetal pulmonary veins better in the second and early-third trimester of pregnancy ,and three-dimensional reconstruction of the image is more intuitive ,helpful to understand the spatial relationship.

Objective: To summarize the major progress in medical image registration in recent years. Furthermore, based on the recent advances in this field, this paper can provide a reference in following domains: three-dimensional medical image reconstruction, medical image visualization, quantitative analysis. Methods: Firstly, referring to a myriad of latest papers on medical image registration. Secondly, analyzing traits and exiting problems of techniques which presented in those papers. Finally, putting forward some efficient methods for solving these problems. Results: This paper compares the characteristics of some typical algorithms and its application and looks forward to the future research work. Conclusion: Using optimization strategy to improve the quality of image registration and studying on non-rigid image registration are the directions for future research in medical image registration field.

Objective To investigate effect of hs-CRP,IL-6, TNF-αand ICAM-1 in patients with coronary heart disease by Guanxinshengmaiyin. Methods 120 patients with coronary heart disease were selected and randomly divided into two groups.60 cases in control group were treated by routine treatment, 60 cases in experiment group were on the base of control group with Guanxinshengmaiyin,1 month for a course.hs-CRP, IL-6, TNF-αand ICAM-1 levels, clinical efficacy, electrocardiogram ( ECG ) and adverse reactions were compared after treatment.Results Compared with before treatment, hs-CRP, IL-6, TNF-αand ICAM-1 levels were lower(P<0.05).Compared with control group, the serum hs-CRP, IL-6, TNF-αand ICAM-1 levels were lower ( P<0.05 ) , the total efficiency ( 80.00%) was higher ( P<0.05 ) , the depth of T wave of lead II and lead III were lower (P<0.05),the adverse reactions occur rate was lower(P<0.05).Conclusion Guanxinshengmaiyin can reduce serum hs-CRP, IL-6, TNF-αand ICAM-1 levels in patients with coronary heart disease, and has good clinical curative effect, less adverse reaction.